Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS06840 and LRK53_RS06845 are separated by 159 nucleotides LRK53_RS06845 and LRK53_RS06850 are separated by 158 nucleotides LRK53_RS06850 and LRK53_RS06855 are separated by 13 nucleotides
LRK53_RS06840: LRK53_RS06840 - tRNA guanosine(34) transglycosylase Tgt, at 1,544,527 to 1,545,654
_RS06840
LRK53_RS06845: LRK53_RS06845 - preprotein translocase subunit YajC, at 1,545,814 to 1,546,146
_RS06845
LRK53_RS06850: LRK53_RS06850 - protein translocase subunit SecD, at 1,546,305 to 1,548,188
_RS06850
LRK53_RS06855: LRK53_RS06855 - protein translocase subunit SecF, at 1,548,202 to 1,549,164
_RS06855
Position (kb)
1546
1547
1548
1549 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1545.406 kb on + strand, within LRK53_RS06840 at 1545.406 kb on + strand, within LRK53_RS06840 at 1545.406 kb on + strand, within LRK53_RS06840 at 1545.406 kb on + strand, within LRK53_RS06840 at 1545.407 kb on - strand, within LRK53_RS06840 at 1545.407 kb on - strand, within LRK53_RS06840 at 1545.448 kb on + strand, within LRK53_RS06840 at 1545.448 kb on + strand, within LRK53_RS06840 at 1545.448 kb on + strand, within LRK53_RS06840 at 1545.448 kb on + strand, within LRK53_RS06840 at 1545.448 kb on + strand, within LRK53_RS06840 at 1545.449 kb on - strand, within LRK53_RS06840 at 1545.449 kb on - strand, within LRK53_RS06840 at 1545.449 kb on - strand, within LRK53_RS06840 at 1545.449 kb on - strand, within LRK53_RS06840 at 1545.479 kb on - strand, within LRK53_RS06840 at 1545.547 kb on + strand at 1545.551 kb on - strand at 1545.652 kb on + strand at 1545.745 kb on + strand at 1545.795 kb on + strand at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1546.181 kb on + strand at 1546.181 kb on + strand at 1546.232 kb on + strand at 1547.460 kb on + strand, within LRK53_RS06850 at 1548.186 kb on + strand at 1548.186 kb on + strand at 1548.198 kb on + strand at 1548.198 kb on + strand at 1548.198 kb on + strand at 1549.174 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 1,545,406 + LRK53_RS06840 0.78 +0.2 1,545,406 + LRK53_RS06840 0.78 +0.1 1,545,406 + LRK53_RS06840 0.78 -0.8 1,545,406 + LRK53_RS06840 0.78 +0.2 1,545,407 - LRK53_RS06840 0.78 -0.8 1,545,407 - LRK53_RS06840 0.78 -0.8 1,545,448 + LRK53_RS06840 0.82 -0.3 1,545,448 + LRK53_RS06840 0.82 -0.3 1,545,448 + LRK53_RS06840 0.82 -0.1 1,545,448 + LRK53_RS06840 0.82 +0.0 1,545,448 + LRK53_RS06840 0.82 +2.2 1,545,449 - LRK53_RS06840 0.82 +1.2 1,545,449 - LRK53_RS06840 0.82 -0.8 1,545,449 - LRK53_RS06840 0.82 +2.2 1,545,449 - LRK53_RS06840 0.82 +0.2 1,545,479 - LRK53_RS06840 0.84 +0.7 1,545,547 + +1.2 1,545,551 - -1.3 1,545,652 + +0.0 1,545,745 + -1.3 1,545,795 + -1.3 1,545,878 + LRK53_RS06845 0.19 -0.2 1,545,878 + LRK53_RS06845 0.19 +0.6 1,545,878 + LRK53_RS06845 0.19 -1.5 1,545,878 + LRK53_RS06845 0.19 +0.2 1,545,878 + LRK53_RS06845 0.19 -1.6 1,545,878 + LRK53_RS06845 0.19 +0.5 1,545,878 + LRK53_RS06845 0.19 -0.3 1,545,919 + LRK53_RS06845 0.32 -0.3 1,545,919 + LRK53_RS06845 0.32 -1.1 1,545,919 + LRK53_RS06845 0.32 +0.6 1,545,919 + LRK53_RS06845 0.32 -1.8 1,545,919 + LRK53_RS06845 0.32 +1.2 1,545,919 + LRK53_RS06845 0.32 -0.3 1,545,919 + LRK53_RS06845 0.32 +0.2 1,545,919 + LRK53_RS06845 0.32 -2.1 1,545,919 + LRK53_RS06845 0.32 +0.8 1,545,919 + LRK53_RS06845 0.32 -0.3 1,545,919 + LRK53_RS06845 0.32 -1.3 1,545,919 + LRK53_RS06845 0.32 -0.8 1,545,919 + LRK53_RS06845 0.32 +1.2 1,545,919 + LRK53_RS06845 0.32 +0.2 1,545,919 + LRK53_RS06845 0.32 +0.7 1,546,181 + -1.3 1,546,181 + -2.2 1,546,232 + -0.1 1,547,460 + LRK53_RS06850 0.61 -1.4 1,548,186 + +0.8 1,548,186 + -2.3 1,548,198 + +0.2 1,548,198 + -0.3 1,548,198 + +0.0 1,549,174 - -0.4
Or see this region's nucleotide sequence