Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS06765 and LRK53_RS06770 are separated by 1 nucleotides LRK53_RS06770 and LRK53_RS06775 overlap by 20 nucleotides LRK53_RS06775 and LRK53_RS06780 are separated by 55 nucleotides LRK53_RS06780 and LRK53_RS06785 are separated by 216 nucleotides
LRK53_RS06765: LRK53_RS06765 - phenylalanine--tRNA ligase subunit beta, at 1,531,127 to 1,533,505
_RS06765
LRK53_RS06770: LRK53_RS06770 - integration host factor subunit alpha, at 1,533,507 to 1,533,806
_RS06770
LRK53_RS06775: LRK53_RS06775 - MerR family transcriptional regulator, at 1,533,787 to 1,534,143
_RS06775
LRK53_RS06780: LRK53_RS06780 - tRNA-Pro, at 1,534,199 to 1,534,275
_RS06780
LRK53_RS06785: LRK53_RS06785 - zeta toxin family protein, at 1,534,492 to 1,535,205
_RS06785
Position (kb)
1533
1534 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1533.594 kb on + strand, within LRK53_RS06770 at 1533.595 kb on - strand, within LRK53_RS06770 at 1533.595 kb on - strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.772 kb on + strand, within LRK53_RS06770 at 1533.773 kb on - strand, within LRK53_RS06770 at 1533.773 kb on - strand, within LRK53_RS06770 at 1533.773 kb on - strand, within LRK53_RS06770 at 1533.773 kb on - strand, within LRK53_RS06770 at 1533.773 kb on - strand, within LRK53_RS06770 at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.786 kb on + strand at 1533.804 kb on + strand at 1534.182 kb on + strand at 1534.284 kb on - strand at 1534.288 kb on + strand at 1534.288 kb on + strand at 1534.288 kb on + strand at 1534.288 kb on + strand at 1534.289 kb on - strand at 1534.289 kb on - strand at 1534.289 kb on - strand at 1534.289 kb on - strand at 1534.289 kb on - strand at 1534.289 kb on - strand at 1534.289 kb on - strand at 1534.289 kb on - strand at 1534.341 kb on + strand at 1534.341 kb on + strand at 1534.341 kb on + strand at 1534.341 kb on + strand at 1534.341 kb on + strand at 1534.341 kb on + strand at 1534.341 kb on + strand at 1534.342 kb on - strand at 1534.342 kb on - strand at 1534.342 kb on - strand at 1534.342 kb on - strand at 1534.342 kb on - strand at 1534.342 kb on - strand at 1534.377 kb on - strand at 1534.377 kb on - strand at 1534.377 kb on - strand at 1534.377 kb on - strand at 1534.444 kb on + strand at 1534.444 kb on + strand at 1534.444 kb on + strand at 1534.444 kb on + strand at 1534.444 kb on + strand at 1534.444 kb on + strand at 1534.444 kb on + strand at 1534.444 kb on + strand at 1534.445 kb on - strand at 1534.445 kb on - strand at 1534.445 kb on - strand at 1534.445 kb on - strand at 1534.445 kb on - strand at 1534.445 kb on - strand at 1534.447 kb on + strand at 1534.447 kb on + strand at 1534.447 kb on + strand at 1534.447 kb on + strand at 1534.447 kb on + strand at 1534.447 kb on + strand at 1534.447 kb on + strand at 1534.447 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.448 kb on - strand at 1534.466 kb on + strand at 1534.467 kb on - strand at 1534.467 kb on - strand at 1534.467 kb on - strand at 1534.467 kb on - strand at 1534.582 kb on + strand, within LRK53_RS06785 at 1534.582 kb on + strand, within LRK53_RS06785 at 1534.582 kb on + strand, within LRK53_RS06785 at 1534.582 kb on + strand, within LRK53_RS06785 at 1534.583 kb on - strand, within LRK53_RS06785 at 1534.583 kb on - strand, within LRK53_RS06785 at 1534.583 kb on - strand, within LRK53_RS06785 at 1534.638 kb on + strand, within LRK53_RS06785 at 1534.726 kb on + strand, within LRK53_RS06785 at 1534.726 kb on + strand, within LRK53_RS06785 at 1534.726 kb on + strand, within LRK53_RS06785 at 1534.726 kb on + strand, within LRK53_RS06785 at 1534.726 kb on + strand, within LRK53_RS06785 at 1534.726 kb on + strand, within LRK53_RS06785 at 1534.726 kb on + strand, within LRK53_RS06785 at 1534.726 kb on + strand, within LRK53_RS06785 at 1534.726 kb on + strand, within LRK53_RS06785 at 1534.726 kb on + strand, within LRK53_RS06785 at 1534.726 kb on + strand, within LRK53_RS06785 at 1534.727 kb on - strand, within LRK53_RS06785 at 1534.727 kb on - strand, within LRK53_RS06785 at 1534.727 kb on - strand, within LRK53_RS06785 at 1534.727 kb on - strand, within LRK53_RS06785 at 1534.727 kb on - strand, within LRK53_RS06785 at 1534.727 kb on - strand, within LRK53_RS06785 at 1534.727 kb on - strand, within LRK53_RS06785 at 1534.727 kb on - strand, within LRK53_RS06785 at 1534.727 kb on - strand, within LRK53_RS06785
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 1,533,594 + LRK53_RS06770 0.29 +0.2 1,533,595 - LRK53_RS06770 0.29 -0.3 1,533,595 - LRK53_RS06770 0.29 +1.2 1,533,772 + LRK53_RS06770 0.88 -2.7 1,533,772 + LRK53_RS06770 0.88 -0.6 1,533,772 + LRK53_RS06770 0.88 -1.3 1,533,772 + LRK53_RS06770 0.88 -1.2 1,533,772 + LRK53_RS06770 0.88 -0.8 1,533,772 + LRK53_RS06770 0.88 -0.3 1,533,772 + LRK53_RS06770 0.88 -0.9 1,533,772 + LRK53_RS06770 0.88 +0.2 1,533,772 + LRK53_RS06770 0.88 -0.3 1,533,772 + LRK53_RS06770 0.88 -0.8 1,533,772 + LRK53_RS06770 0.88 +0.2 1,533,772 + LRK53_RS06770 0.88 -0.9 1,533,772 + LRK53_RS06770 0.88 +0.2 1,533,772 + LRK53_RS06770 0.88 -1.1 1,533,772 + LRK53_RS06770 0.88 -0.8 1,533,772 + LRK53_RS06770 0.88 -0.8 1,533,772 + LRK53_RS06770 0.88 +0.2 1,533,772 + LRK53_RS06770 0.88 -1.9 1,533,772 + LRK53_RS06770 0.88 -0.8 1,533,772 + LRK53_RS06770 0.88 -0.8 1,533,772 + LRK53_RS06770 0.88 -1.6 1,533,772 + LRK53_RS06770 0.88 -0.3 1,533,772 + LRK53_RS06770 0.88 -1.3 1,533,772 + LRK53_RS06770 0.88 -1.1 1,533,773 - LRK53_RS06770 0.89 +0.2 1,533,773 - LRK53_RS06770 0.89 -0.3 1,533,773 - LRK53_RS06770 0.89 -0.6 1,533,773 - LRK53_RS06770 0.89 -0.3 1,533,773 - LRK53_RS06770 0.89 -2.5 1,533,786 + -0.2 1,533,786 + -0.3 1,533,786 + -1.6 1,533,786 + +1.2 1,533,786 + +0.5 1,533,786 + -0.5 1,533,786 + -1.1 1,533,786 + -1.5 1,533,786 + -0.6 1,533,786 + -0.8 1,533,786 + -0.6 1,533,786 + -1.1 1,533,786 + +1.2 1,533,804 + +0.2 1,534,182 + +0.2 1,534,284 - -0.6 1,534,288 + +0.2 1,534,288 + +1.2 1,534,288 + +2.2 1,534,288 + -0.3 1,534,289 - -2.1 1,534,289 - -1.3 1,534,289 - +0.0 1,534,289 - +0.2 1,534,289 - +0.7 1,534,289 - +0.8 1,534,289 - +0.2 1,534,289 - -2.1 1,534,341 + -1.2 1,534,341 + +0.2 1,534,341 + +1.8 1,534,341 + -1.1 1,534,341 + -0.8 1,534,341 + -1.9 1,534,341 + -0.9 1,534,342 - -0.6 1,534,342 - +1.2 1,534,342 - -3.1 1,534,342 - -0.2 1,534,342 - -0.1 1,534,342 - -1.6 1,534,377 - +3.2 1,534,377 - -1.1 1,534,377 - -0.3 1,534,377 - -1.3 1,534,444 + +1.2 1,534,444 + +2.2 1,534,444 + -1.5 1,534,444 + -1.3 1,534,444 + +0.9 1,534,444 + +0.1 1,534,444 + -0.0 1,534,444 + -1.2 1,534,445 - -0.3 1,534,445 - -1.0 1,534,445 - -0.6 1,534,445 - +0.1 1,534,445 - -0.8 1,534,445 - -0.3 1,534,447 + -1.1 1,534,447 + -0.8 1,534,447 + +2.2 1,534,447 + +0.1 1,534,447 + -1.3 1,534,447 + -1.1 1,534,447 + -1.1 1,534,447 - -1.1 1,534,448 - -0.3 1,534,448 - -0.1 1,534,448 - +0.7 1,534,448 - -1.0 1,534,448 - -0.8 1,534,448 - -0.4 1,534,448 - -1.1 1,534,448 - -0.9 1,534,448 - -1.5 1,534,448 - -2.1 1,534,448 - -0.9 1,534,448 - -0.3 1,534,448 - +0.5 1,534,448 - -1.3 1,534,448 - -0.5 1,534,448 - +1.2 1,534,448 - -0.2 1,534,448 - +0.2 1,534,448 - -2.2 1,534,466 + -1.3 1,534,467 - +0.9 1,534,467 - +0.8 1,534,467 - -0.6 1,534,467 - -1.0 1,534,582 + LRK53_RS06785 0.13 -0.3 1,534,582 + LRK53_RS06785 0.13 -1.3 1,534,582 + LRK53_RS06785 0.13 +1.2 1,534,582 + LRK53_RS06785 0.13 -0.1 1,534,583 - LRK53_RS06785 0.13 -1.3 1,534,583 - LRK53_RS06785 0.13 -0.3 1,534,583 - LRK53_RS06785 0.13 -1.2 1,534,638 + LRK53_RS06785 0.20 -0.0 1,534,726 + LRK53_RS06785 0.33 -0.9 1,534,726 + LRK53_RS06785 0.33 -0.7 1,534,726 + LRK53_RS06785 0.33 -0.1 1,534,726 + LRK53_RS06785 0.33 -0.3 1,534,726 + LRK53_RS06785 0.33 -1.3 1,534,726 + LRK53_RS06785 0.33 +0.7 1,534,726 + LRK53_RS06785 0.33 -0.8 1,534,726 + LRK53_RS06785 0.33 +0.6 1,534,726 + LRK53_RS06785 0.33 -0.2 1,534,726 + LRK53_RS06785 0.33 -0.8 1,534,726 + LRK53_RS06785 0.33 -0.3 1,534,727 - LRK53_RS06785 0.33 -0.2 1,534,727 - LRK53_RS06785 0.33 +0.0 1,534,727 - LRK53_RS06785 0.33 -0.7 1,534,727 - LRK53_RS06785 0.33 +1.2 1,534,727 - LRK53_RS06785 0.33 -1.0 1,534,727 - LRK53_RS06785 0.33 -0.3 1,534,727 - LRK53_RS06785 0.33 -0.8 1,534,727 - LRK53_RS06785 0.33 -0.3 1,534,727 - LRK53_RS06785 0.33 +1.2
Or see this region's nucleotide sequence