Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04630 and LRK53_RS04635 are separated by 183 nucleotides LRK53_RS04635 and LRK53_RS04640 are separated by 77 nucleotides LRK53_RS04640 and LRK53_RS04645 overlap by 4 nucleotides LRK53_RS04645 and LRK53_RS04650 are separated by 151 nucleotides
LRK53_RS04630: LRK53_RS04630 - hypothetical protein, at 1,078,980 to 1,079,849
_RS04630
LRK53_RS04635: LRK53_RS04635 - M20/M25/M40 family metallo-hydrolase, at 1,080,033 to 1,080,758
_RS04635
LRK53_RS04640: LRK53_RS04640 - helix-turn-helix domain-containing protein, at 1,080,836 to 1,081,186
_RS04640
LRK53_RS04645: LRK53_RS04645 - DUF6516 family protein, at 1,081,183 to 1,081,548
_RS04645
LRK53_RS04650: LRK53_RS04650 - M20/M25/M40 family metallo-hydrolase, at 1,081,700 to 1,083,097
_RS04650
Position (kb)
1080
1081
1082 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1079.874 kb on - strand at 1079.910 kb on + strand at 1079.910 kb on + strand at 1079.931 kb on + strand at 1079.931 kb on + strand at 1079.988 kb on + strand at 1079.988 kb on + strand at 1080.011 kb on + strand at 1080.011 kb on + strand at 1080.012 kb on - strand at 1080.012 kb on - strand at 1080.012 kb on - strand at 1080.012 kb on - strand at 1080.012 kb on - strand at 1080.012 kb on - strand at 1080.012 kb on - strand at 1080.012 kb on - strand at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.191 kb on - strand, within LRK53_RS04635 at 1080.279 kb on + strand, within LRK53_RS04635 at 1080.279 kb on + strand, within LRK53_RS04635 at 1080.279 kb on + strand, within LRK53_RS04635 at 1080.279 kb on + strand, within LRK53_RS04635 at 1080.280 kb on - strand, within LRK53_RS04635 at 1080.280 kb on - strand, within LRK53_RS04635 at 1080.327 kb on + strand, within LRK53_RS04635 at 1080.327 kb on + strand, within LRK53_RS04635 at 1080.327 kb on + strand, within LRK53_RS04635 at 1080.327 kb on + strand, within LRK53_RS04635 at 1080.327 kb on + strand, within LRK53_RS04635 at 1080.328 kb on - strand, within LRK53_RS04635 at 1080.328 kb on - strand, within LRK53_RS04635 at 1080.328 kb on - strand, within LRK53_RS04635 at 1080.328 kb on - strand, within LRK53_RS04635 at 1080.328 kb on - strand, within LRK53_RS04635 at 1080.330 kb on + strand, within LRK53_RS04635 at 1080.330 kb on + strand, within LRK53_RS04635 at 1080.330 kb on + strand, within LRK53_RS04635 at 1080.330 kb on + strand, within LRK53_RS04635 at 1080.330 kb on + strand, within LRK53_RS04635 at 1080.330 kb on + strand, within LRK53_RS04635 at 1080.330 kb on + strand, within LRK53_RS04635 at 1080.330 kb on + strand, within LRK53_RS04635 at 1080.331 kb on - strand, within LRK53_RS04635 at 1080.331 kb on - strand, within LRK53_RS04635 at 1080.331 kb on - strand, within LRK53_RS04635 at 1080.331 kb on - strand, within LRK53_RS04635 at 1080.331 kb on - strand, within LRK53_RS04635 at 1080.480 kb on - strand, within LRK53_RS04635 at 1080.480 kb on - strand, within LRK53_RS04635 at 1080.480 kb on - strand, within LRK53_RS04635 at 1080.511 kb on + strand, within LRK53_RS04635 at 1080.700 kb on + strand at 1080.700 kb on + strand at 1080.701 kb on - strand at 1080.772 kb on + strand at 1080.772 kb on + strand at 1080.773 kb on - strand at 1080.773 kb on - strand at 1080.773 kb on - strand at 1080.773 kb on - strand at 1080.773 kb on - strand at 1080.773 kb on - strand at 1080.773 kb on - strand at 1080.797 kb on + strand at 1080.798 kb on - strand at 1080.824 kb on + strand at 1080.825 kb on - strand at 1080.837 kb on + strand at 1080.837 kb on + strand at 1080.837 kb on + strand at 1080.838 kb on - strand at 1080.838 kb on - strand at 1080.838 kb on - strand at 1080.918 kb on + strand, within LRK53_RS04640 at 1080.918 kb on + strand, within LRK53_RS04640 at 1080.918 kb on + strand, within LRK53_RS04640 at 1080.919 kb on - strand, within LRK53_RS04640 at 1081.204 kb on + strand at 1081.204 kb on + strand at 1081.204 kb on + strand at 1081.204 kb on + strand at 1081.262 kb on + strand, within LRK53_RS04645 at 1081.263 kb on - strand, within LRK53_RS04645 at 1081.267 kb on + strand, within LRK53_RS04645 at 1081.267 kb on + strand, within LRK53_RS04645 at 1081.268 kb on - strand, within LRK53_RS04645 at 1081.375 kb on + strand, within LRK53_RS04645 at 1081.412 kb on + strand, within LRK53_RS04645 at 1081.413 kb on - strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.417 kb on + strand, within LRK53_RS04645 at 1081.418 kb on - strand, within LRK53_RS04645 at 1081.418 kb on - strand, within LRK53_RS04645 at 1081.418 kb on - strand, within LRK53_RS04645 at 1081.418 kb on - strand, within LRK53_RS04645 at 1081.418 kb on - strand, within LRK53_RS04645 at 1081.418 kb on - strand, within LRK53_RS04645 at 1081.418 kb on - strand, within LRK53_RS04645 at 1081.418 kb on - strand, within LRK53_RS04645 at 1081.418 kb on - strand, within LRK53_RS04645 at 1081.418 kb on - strand, within LRK53_RS04645 at 1081.418 kb on - strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.493 kb on + strand, within LRK53_RS04645 at 1081.494 kb on - strand, within LRK53_RS04645 at 1081.494 kb on - strand, within LRK53_RS04645 at 1081.494 kb on - strand, within LRK53_RS04645 at 1081.494 kb on - strand, within LRK53_RS04645 at 1081.494 kb on - strand, within LRK53_RS04645 at 1081.494 kb on - strand, within LRK53_RS04645 at 1081.494 kb on - strand, within LRK53_RS04645 at 1081.494 kb on - strand, within LRK53_RS04645 at 1081.494 kb on - strand, within LRK53_RS04645 at 1081.553 kb on - strand at 1081.553 kb on - strand at 1081.553 kb on - strand at 1081.553 kb on - strand at 1081.553 kb on - strand at 1081.554 kb on + strand at 1081.554 kb on + strand at 1081.554 kb on + strand at 1081.554 kb on + strand at 1081.555 kb on - strand at 1081.555 kb on - strand at 1081.555 kb on - strand at 1081.555 kb on - strand at 1081.555 kb on - strand at 1081.557 kb on - strand at 1081.557 kb on - strand at 1081.557 kb on - strand at 1081.557 kb on - strand at 1081.640 kb on + strand at 1081.640 kb on + strand at 1081.640 kb on + strand at 1081.640 kb on + strand at 1081.640 kb on + strand at 1081.640 kb on + strand at 1081.640 kb on + strand at 1081.640 kb on + strand at 1081.640 kb on + strand at 1081.641 kb on - strand at 1081.641 kb on - strand at 1081.641 kb on - strand at 1081.830 kb on + strand at 1081.831 kb on - strand at 1082.178 kb on + strand, within LRK53_RS04650
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 1,079,874 - -0.0 1,079,910 + +1.3 1,079,910 + -0.3 1,079,931 + -0.0 1,079,931 + +0.3 1,079,988 + -0.3 1,079,988 + -0.7 1,080,011 + -0.1 1,080,011 + -1.1 1,080,012 - -0.7 1,080,012 - +1.3 1,080,012 - +0.3 1,080,012 - -0.4 1,080,012 - +2.3 1,080,012 - -0.3 1,080,012 - -0.7 1,080,012 - +0.3 1,080,191 - LRK53_RS04635 0.22 +0.3 1,080,191 - LRK53_RS04635 0.22 +1.3 1,080,191 - LRK53_RS04635 0.22 -0.5 1,080,191 - LRK53_RS04635 0.22 -0.7 1,080,191 - LRK53_RS04635 0.22 -0.3 1,080,191 - LRK53_RS04635 0.22 -1.7 1,080,191 - LRK53_RS04635 0.22 +0.1 1,080,191 - LRK53_RS04635 0.22 +1.3 1,080,191 - LRK53_RS04635 0.22 -1.0 1,080,191 - LRK53_RS04635 0.22 -2.0 1,080,191 - LRK53_RS04635 0.22 +0.3 1,080,191 - LRK53_RS04635 0.22 +1.3 1,080,191 - LRK53_RS04635 0.22 -0.3 1,080,191 - LRK53_RS04635 0.22 -1.2 1,080,191 - LRK53_RS04635 0.22 -0.2 1,080,191 - LRK53_RS04635 0.22 +1.3 1,080,191 - LRK53_RS04635 0.22 -0.7 1,080,191 - LRK53_RS04635 0.22 +0.5 1,080,191 - LRK53_RS04635 0.22 -0.3 1,080,191 - LRK53_RS04635 0.22 -1.5 1,080,191 - LRK53_RS04635 0.22 +0.5 1,080,279 + LRK53_RS04635 0.34 +0.3 1,080,279 + LRK53_RS04635 0.34 -0.3 1,080,279 + LRK53_RS04635 0.34 -0.9 1,080,279 + LRK53_RS04635 0.34 +1.3 1,080,280 - LRK53_RS04635 0.34 -0.3 1,080,280 - LRK53_RS04635 0.34 -0.7 1,080,327 + LRK53_RS04635 0.40 -0.0 1,080,327 + LRK53_RS04635 0.40 -0.3 1,080,327 + LRK53_RS04635 0.40 -1.2 1,080,327 + LRK53_RS04635 0.40 -2.4 1,080,327 + LRK53_RS04635 0.40 -1.0 1,080,328 - LRK53_RS04635 0.41 +0.7 1,080,328 - LRK53_RS04635 0.41 +1.3 1,080,328 - LRK53_RS04635 0.41 -1.5 1,080,328 - LRK53_RS04635 0.41 -0.1 1,080,328 - LRK53_RS04635 0.41 +0.1 1,080,330 + LRK53_RS04635 0.41 +1.9 1,080,330 + LRK53_RS04635 0.41 -0.2 1,080,330 + LRK53_RS04635 0.41 -1.4 1,080,330 + LRK53_RS04635 0.41 -0.7 1,080,330 + LRK53_RS04635 0.41 -0.5 1,080,330 + LRK53_RS04635 0.41 +0.5 1,080,330 + LRK53_RS04635 0.41 -0.7 1,080,330 + LRK53_RS04635 0.41 +0.7 1,080,331 - LRK53_RS04635 0.41 -0.1 1,080,331 - LRK53_RS04635 0.41 +1.3 1,080,331 - LRK53_RS04635 0.41 +0.1 1,080,331 - LRK53_RS04635 0.41 -0.3 1,080,331 - LRK53_RS04635 0.41 -0.5 1,080,480 - LRK53_RS04635 0.62 -0.3 1,080,480 - LRK53_RS04635 0.62 -0.5 1,080,480 - LRK53_RS04635 0.62 +0.2 1,080,511 + LRK53_RS04635 0.66 +0.7 1,080,700 + -0.0 1,080,700 + +0.3 1,080,701 - -2.3 1,080,772 + -1.0 1,080,772 + -0.7 1,080,773 - -0.3 1,080,773 - -0.7 1,080,773 - -0.9 1,080,773 - -1.5 1,080,773 - -1.6 1,080,773 - -0.0 1,080,773 - +0.2 1,080,797 + +1.3 1,080,798 - -1.7 1,080,824 + +0.3 1,080,825 - +1.3 1,080,837 + -0.6 1,080,837 + +1.0 1,080,837 + -1.9 1,080,838 - +1.3 1,080,838 - +1.3 1,080,838 - +0.3 1,080,918 + LRK53_RS04640 0.23 -2.0 1,080,918 + LRK53_RS04640 0.23 -1.8 1,080,918 + LRK53_RS04640 0.23 -1.5 1,080,919 - LRK53_RS04640 0.24 -0.1 1,081,204 + -0.7 1,081,204 + -0.7 1,081,204 + -0.0 1,081,204 + +0.3 1,081,262 + LRK53_RS04645 0.22 -0.8 1,081,263 - LRK53_RS04645 0.22 +0.5 1,081,267 + LRK53_RS04645 0.23 +0.7 1,081,267 + LRK53_RS04645 0.23 -0.3 1,081,268 - LRK53_RS04645 0.23 +0.3 1,081,375 + LRK53_RS04645 0.52 -2.1 1,081,412 + LRK53_RS04645 0.63 -0.7 1,081,413 - LRK53_RS04645 0.63 -0.3 1,081,417 + LRK53_RS04645 0.64 -1.7 1,081,417 + LRK53_RS04645 0.64 +0.5 1,081,417 + LRK53_RS04645 0.64 -0.0 1,081,417 + LRK53_RS04645 0.64 -1.3 1,081,417 + LRK53_RS04645 0.64 +2.3 1,081,417 + LRK53_RS04645 0.64 -2.2 1,081,417 + LRK53_RS04645 0.64 +0.3 1,081,417 + LRK53_RS04645 0.64 -0.3 1,081,417 + LRK53_RS04645 0.64 +2.3 1,081,417 + LRK53_RS04645 0.64 +0.8 1,081,417 + LRK53_RS04645 0.64 -4.1 1,081,417 + LRK53_RS04645 0.64 -0.6 1,081,417 + LRK53_RS04645 0.64 +1.3 1,081,417 + LRK53_RS04645 0.64 -0.8 1,081,418 - LRK53_RS04645 0.64 +0.0 1,081,418 - LRK53_RS04645 0.64 -0.5 1,081,418 - LRK53_RS04645 0.64 -1.0 1,081,418 - LRK53_RS04645 0.64 +0.3 1,081,418 - LRK53_RS04645 0.64 +0.5 1,081,418 - LRK53_RS04645 0.64 -1.0 1,081,418 - LRK53_RS04645 0.64 +1.3 1,081,418 - LRK53_RS04645 0.64 +1.3 1,081,418 - LRK53_RS04645 0.64 -1.6 1,081,418 - LRK53_RS04645 0.64 -0.8 1,081,418 - LRK53_RS04645 0.64 -0.3 1,081,493 + LRK53_RS04645 0.85 +0.3 1,081,493 + LRK53_RS04645 0.85 +1.3 1,081,493 + LRK53_RS04645 0.85 -1.1 1,081,493 + LRK53_RS04645 0.85 -0.2 1,081,493 + LRK53_RS04645 0.85 -0.3 1,081,493 + LRK53_RS04645 0.85 -0.7 1,081,493 + LRK53_RS04645 0.85 -1.0 1,081,493 + LRK53_RS04645 0.85 +0.3 1,081,493 + LRK53_RS04645 0.85 -1.7 1,081,493 + LRK53_RS04645 0.85 -0.0 1,081,493 + LRK53_RS04645 0.85 -1.9 1,081,493 + LRK53_RS04645 0.85 +0.1 1,081,493 + LRK53_RS04645 0.85 +0.1 1,081,494 - LRK53_RS04645 0.85 +0.5 1,081,494 - LRK53_RS04645 0.85 +0.1 1,081,494 - LRK53_RS04645 0.85 -1.8 1,081,494 - LRK53_RS04645 0.85 -0.5 1,081,494 - LRK53_RS04645 0.85 -1.0 1,081,494 - LRK53_RS04645 0.85 +1.3 1,081,494 - LRK53_RS04645 0.85 -0.3 1,081,494 - LRK53_RS04645 0.85 -1.1 1,081,494 - LRK53_RS04645 0.85 -1.2 1,081,553 - -0.5 1,081,553 - +0.1 1,081,553 - +0.3 1,081,553 - -0.1 1,081,553 - +0.2 1,081,554 + -2.1 1,081,554 + -1.6 1,081,554 + +1.3 1,081,554 + +0.3 1,081,555 - -0.4 1,081,555 - +0.1 1,081,555 - -1.9 1,081,555 - -0.3 1,081,555 - -0.0 1,081,557 - +0.3 1,081,557 - +0.3 1,081,557 - +1.7 1,081,557 - -0.5 1,081,640 + +1.3 1,081,640 + -1.3 1,081,640 + -0.0 1,081,640 + +0.2 1,081,640 + +1.3 1,081,640 + -0.1 1,081,640 + +0.7 1,081,640 + +0.9 1,081,640 + -1.0 1,081,641 - -0.5 1,081,641 - -1.0 1,081,641 - -1.3 1,081,830 + -1.3 1,081,831 - +0.1 1,082,178 + LRK53_RS04650 0.34 +0.7
Or see this region's nucleotide sequence