Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03720 and LRK53_RS03725 are separated by 139 nucleotides LRK53_RS03725 and LRK53_RS03730 overlap by 4 nucleotides LRK53_RS03730 and LRK53_RS03735 are separated by 160 nucleotides
LRK53_RS03720: LRK53_RS03720 - permease-like cell division protein FtsX, at 877,842 to 878,822
_RS03720
LRK53_RS03725: LRK53_RS03725 - response regulator, at 878,962 to 879,804
_RS03725
LRK53_RS03730: LRK53_RS03730 - uracil-DNA glycosylase, at 879,801 to 880,523
_RS03730
LRK53_RS03735: LRK53_RS03735 - RNA polymerase sigma factor RpoH, at 880,684 to 881,550
_RS03735
Position (kb) 879
880
881 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 878.878 kb on + strand at 878.879 kb on - strand at 878.879 kb on - strand at 878.879 kb on - strand at 878.879 kb on - strand at 878.888 kb on + strand at 878.888 kb on + strand at 878.888 kb on + strand at 878.888 kb on + strand at 878.889 kb on - strand at 878.956 kb on + strand at 878.956 kb on + strand at 878.956 kb on + strand at 878.957 kb on - strand at 878.957 kb on - strand at 878.957 kb on - strand at 878.967 kb on + strand at 878.967 kb on + strand at 878.967 kb on + strand at 878.967 kb on + strand at 878.967 kb on + strand at 878.968 kb on - strand at 878.968 kb on - strand at 878.968 kb on - strand at 878.973 kb on + strand at 878.973 kb on + strand at 878.973 kb on + strand at 878.973 kb on + strand at 878.973 kb on + strand at 878.973 kb on + strand at 878.973 kb on + strand at 878.973 kb on + strand at 878.974 kb on - strand at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.226 kb on + strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.227 kb on - strand, within LRK53_RS03725 at 879.304 kb on + strand, within LRK53_RS03725 at 879.304 kb on + strand, within LRK53_RS03725 at 879.304 kb on + strand, within LRK53_RS03725 at 879.305 kb on - strand, within LRK53_RS03725 at 879.305 kb on - strand, within LRK53_RS03725 at 879.305 kb on - strand, within LRK53_RS03725 at 879.355 kb on + strand, within LRK53_RS03725 at 879.355 kb on + strand, within LRK53_RS03725 at 879.356 kb on - strand, within LRK53_RS03725 at 879.394 kb on + strand, within LRK53_RS03725 at 879.394 kb on + strand, within LRK53_RS03725 at 879.394 kb on + strand, within LRK53_RS03725 at 879.394 kb on + strand, within LRK53_RS03725 at 879.394 kb on + strand, within LRK53_RS03725 at 879.394 kb on + strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.395 kb on - strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.470 kb on + strand, within LRK53_RS03725 at 879.471 kb on - strand, within LRK53_RS03725 at 879.471 kb on - strand, within LRK53_RS03725 at 879.471 kb on - strand, within LRK53_RS03725 at 879.471 kb on - strand, within LRK53_RS03725 at 879.471 kb on - strand, within LRK53_RS03725 at 879.471 kb on - strand, within LRK53_RS03725 at 879.471 kb on - strand, within LRK53_RS03725 at 879.848 kb on + strand at 879.858 kb on + strand at 879.858 kb on + strand at 879.858 kb on + strand at 879.858 kb on + strand at 879.859 kb on - strand at 879.859 kb on - strand at 879.859 kb on - strand at 879.859 kb on - strand at 879.859 kb on - strand at 879.930 kb on + strand, within LRK53_RS03730 at 879.930 kb on + strand, within LRK53_RS03730 at 879.930 kb on + strand, within LRK53_RS03730 at 879.930 kb on + strand, within LRK53_RS03730 at 879.931 kb on - strand, within LRK53_RS03730 at 879.931 kb on - strand, within LRK53_RS03730 at 879.931 kb on - strand, within LRK53_RS03730 at 879.931 kb on - strand, within LRK53_RS03730 at 879.931 kb on - strand, within LRK53_RS03730 at 880.015 kb on - strand, within LRK53_RS03730 at 880.314 kb on + strand, within LRK53_RS03730 at 880.314 kb on + strand, within LRK53_RS03730 at 880.314 kb on + strand, within LRK53_RS03730 at 880.314 kb on + strand, within LRK53_RS03730 at 880.314 kb on + strand, within LRK53_RS03730 at 880.315 kb on - strand, within LRK53_RS03730 at 880.315 kb on - strand, within LRK53_RS03730 at 880.315 kb on - strand, within LRK53_RS03730 at 880.315 kb on - strand, within LRK53_RS03730 at 880.315 kb on - strand, within LRK53_RS03730 at 880.315 kb on - strand, within LRK53_RS03730 at 880.440 kb on + strand, within LRK53_RS03730 at 880.440 kb on + strand, within LRK53_RS03730 at 880.587 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 878,878 + +1.3 878,879 - -0.7 878,879 - -0.0 878,879 - -0.3 878,879 - +1.7 878,888 + +0.1 878,888 + -1.4 878,888 + +0.3 878,888 + -0.5 878,889 - -0.0 878,956 + -1.5 878,956 + -0.3 878,956 + -1.3 878,957 - -1.5 878,957 - +0.3 878,957 - +0.9 878,967 + +0.7 878,967 + +0.3 878,967 + -1.0 878,967 + -0.3 878,967 + -1.0 878,968 - -0.3 878,968 - +2.3 878,968 - +0.5 878,973 + +0.7 878,973 + +0.3 878,973 + +0.7 878,973 + -0.0 878,973 + +0.7 878,973 + +1.3 878,973 + -1.2 878,973 + -0.9 878,974 - -3.0 879,226 + LRK53_RS03725 0.31 -0.9 879,226 + LRK53_RS03725 0.31 -0.3 879,226 + LRK53_RS03725 0.31 -1.1 879,226 + LRK53_RS03725 0.31 +1.7 879,226 + LRK53_RS03725 0.31 -0.0 879,226 + LRK53_RS03725 0.31 -0.4 879,226 + LRK53_RS03725 0.31 +2.3 879,226 + LRK53_RS03725 0.31 +1.1 879,226 + LRK53_RS03725 0.31 +1.0 879,226 + LRK53_RS03725 0.31 -0.7 879,226 + LRK53_RS03725 0.31 +0.3 879,226 + LRK53_RS03725 0.31 +1.3 879,226 + LRK53_RS03725 0.31 +0.9 879,226 + LRK53_RS03725 0.31 +0.3 879,226 + LRK53_RS03725 0.31 -0.1 879,227 - LRK53_RS03725 0.31 -0.0 879,227 - LRK53_RS03725 0.31 +0.3 879,227 - LRK53_RS03725 0.31 -1.2 879,227 - LRK53_RS03725 0.31 -0.5 879,227 - LRK53_RS03725 0.31 +0.4 879,227 - LRK53_RS03725 0.31 -0.2 879,227 - LRK53_RS03725 0.31 +1.3 879,227 - LRK53_RS03725 0.31 +1.9 879,227 - LRK53_RS03725 0.31 +1.3 879,227 - LRK53_RS03725 0.31 -0.7 879,227 - LRK53_RS03725 0.31 +0.3 879,227 - LRK53_RS03725 0.31 +0.3 879,227 - LRK53_RS03725 0.31 +1.3 879,227 - LRK53_RS03725 0.31 +0.4 879,227 - LRK53_RS03725 0.31 -1.1 879,227 - LRK53_RS03725 0.31 -0.3 879,227 - LRK53_RS03725 0.31 -0.3 879,227 - LRK53_RS03725 0.31 +0.1 879,304 + LRK53_RS03725 0.41 +0.7 879,304 + LRK53_RS03725 0.41 -0.4 879,304 + LRK53_RS03725 0.41 +0.3 879,305 - LRK53_RS03725 0.41 -1.0 879,305 - LRK53_RS03725 0.41 +0.1 879,305 - LRK53_RS03725 0.41 -0.0 879,355 + LRK53_RS03725 0.47 -0.5 879,355 + LRK53_RS03725 0.47 +1.3 879,356 - LRK53_RS03725 0.47 -1.9 879,394 + LRK53_RS03725 0.51 -1.0 879,394 + LRK53_RS03725 0.51 +0.3 879,394 + LRK53_RS03725 0.51 -1.4 879,394 + LRK53_RS03725 0.51 +1.0 879,394 + LRK53_RS03725 0.51 -1.3 879,394 + LRK53_RS03725 0.51 -0.3 879,395 - LRK53_RS03725 0.51 +0.3 879,395 - LRK53_RS03725 0.51 +0.3 879,395 - LRK53_RS03725 0.51 -0.3 879,395 - LRK53_RS03725 0.51 -0.7 879,395 - LRK53_RS03725 0.51 -0.3 879,395 - LRK53_RS03725 0.51 -0.3 879,395 - LRK53_RS03725 0.51 -1.0 879,395 - LRK53_RS03725 0.51 -0.7 879,395 - LRK53_RS03725 0.51 -0.8 879,395 - LRK53_RS03725 0.51 -0.9 879,395 - LRK53_RS03725 0.51 +0.3 879,395 - LRK53_RS03725 0.51 +2.9 879,395 - LRK53_RS03725 0.51 -1.0 879,470 + LRK53_RS03725 0.60 +1.3 879,470 + LRK53_RS03725 0.60 -0.3 879,470 + LRK53_RS03725 0.60 -0.3 879,470 + LRK53_RS03725 0.60 -1.3 879,470 + LRK53_RS03725 0.60 +0.1 879,470 + LRK53_RS03725 0.60 -0.2 879,470 + LRK53_RS03725 0.60 -2.1 879,470 + LRK53_RS03725 0.60 +1.3 879,470 + LRK53_RS03725 0.60 -1.0 879,470 + LRK53_RS03725 0.60 +0.0 879,470 + LRK53_RS03725 0.60 -0.1 879,470 + LRK53_RS03725 0.60 -0.3 879,470 + LRK53_RS03725 0.60 -0.9 879,470 + LRK53_RS03725 0.60 +0.7 879,470 + LRK53_RS03725 0.60 -0.1 879,470 + LRK53_RS03725 0.60 -0.7 879,470 + LRK53_RS03725 0.60 +0.3 879,471 - LRK53_RS03725 0.60 -0.9 879,471 - LRK53_RS03725 0.60 -0.3 879,471 - LRK53_RS03725 0.60 +1.3 879,471 - LRK53_RS03725 0.60 -1.0 879,471 - LRK53_RS03725 0.60 -0.5 879,471 - LRK53_RS03725 0.60 +1.3 879,471 - LRK53_RS03725 0.60 +1.3 879,848 + -1.3 879,858 + +0.7 879,858 + -1.2 879,858 + +2.3 879,858 + -0.8 879,859 - -0.3 879,859 - +0.7 879,859 - -0.9 879,859 - -0.3 879,859 - -0.5 879,930 + LRK53_RS03730 0.18 +0.7 879,930 + LRK53_RS03730 0.18 -1.7 879,930 + LRK53_RS03730 0.18 -0.3 879,930 + LRK53_RS03730 0.18 -1.3 879,931 - LRK53_RS03730 0.18 +0.4 879,931 - LRK53_RS03730 0.18 +1.3 879,931 - LRK53_RS03730 0.18 -0.7 879,931 - LRK53_RS03730 0.18 -1.8 879,931 - LRK53_RS03730 0.18 +0.3 880,015 - LRK53_RS03730 0.30 -0.3 880,314 + LRK53_RS03730 0.71 -2.7 880,314 + LRK53_RS03730 0.71 -0.0 880,314 + LRK53_RS03730 0.71 +0.6 880,314 + LRK53_RS03730 0.71 -1.3 880,314 + LRK53_RS03730 0.71 -0.3 880,315 - LRK53_RS03730 0.71 -1.0 880,315 - LRK53_RS03730 0.71 -1.2 880,315 - LRK53_RS03730 0.71 -0.6 880,315 - LRK53_RS03730 0.71 -0.5 880,315 - LRK53_RS03730 0.71 -1.7 880,315 - LRK53_RS03730 0.71 +0.3 880,440 + LRK53_RS03730 0.88 -0.9 880,440 + LRK53_RS03730 0.88 -0.5 880,587 + -0.2
Or see this region's nucleotide sequence