Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03695 and LRK53_RS03700 are separated by 202 nucleotides LRK53_RS03700 and LRK53_RS03705 are separated by 188 nucleotides LRK53_RS03705 and LRK53_RS03710 are separated by 382 nucleotides
LRK53_RS03695: LRK53_RS03695 - glutamate--cysteine ligase, at 871,052 to 872,419
_RS03695
LRK53_RS03700: LRK53_RS03700 - transcription termination factor Rho, at 872,622 to 874,406
_RS03700
LRK53_RS03705: LRK53_RS03705 - thioredoxin TrxA, at 874,595 to 874,921
_RS03705
LRK53_RS03710: LRK53_RS03710 - DEAD/DEAH box helicase, at 875,304 to 877,055
_RS03710
Position (kb) 872
873
874
875 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 871.626 kb on + strand, within LRK53_RS03695 at 871.626 kb on + strand, within LRK53_RS03695 at 871.626 kb on + strand, within LRK53_RS03695 at 871.626 kb on + strand, within LRK53_RS03695 at 871.626 kb on + strand, within LRK53_RS03695 at 871.626 kb on + strand, within LRK53_RS03695 at 871.626 kb on + strand, within LRK53_RS03695 at 871.626 kb on + strand, within LRK53_RS03695 at 871.626 kb on + strand, within LRK53_RS03695 at 871.627 kb on - strand, within LRK53_RS03695 at 871.627 kb on - strand, within LRK53_RS03695 at 871.627 kb on - strand, within LRK53_RS03695 at 871.627 kb on - strand, within LRK53_RS03695 at 871.627 kb on - strand, within LRK53_RS03695 at 871.627 kb on - strand, within LRK53_RS03695 at 871.627 kb on - strand, within LRK53_RS03695 at 871.627 kb on - strand, within LRK53_RS03695 at 871.627 kb on - strand, within LRK53_RS03695 at 871.627 kb on - strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.647 kb on + strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.648 kb on - strand, within LRK53_RS03695 at 871.656 kb on + strand, within LRK53_RS03695 at 871.657 kb on - strand, within LRK53_RS03695 at 871.657 kb on - strand, within LRK53_RS03695 at 871.752 kb on + strand, within LRK53_RS03695 at 871.752 kb on + strand, within LRK53_RS03695 at 871.752 kb on + strand, within LRK53_RS03695 at 871.752 kb on + strand, within LRK53_RS03695 at 871.752 kb on + strand, within LRK53_RS03695 at 871.753 kb on - strand, within LRK53_RS03695 at 871.753 kb on - strand, within LRK53_RS03695 at 871.753 kb on - strand, within LRK53_RS03695 at 871.753 kb on - strand, within LRK53_RS03695 at 871.753 kb on - strand, within LRK53_RS03695 at 871.753 kb on - strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.935 kb on + strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.936 kb on - strand, within LRK53_RS03695 at 871.953 kb on + strand, within LRK53_RS03695 at 871.953 kb on + strand, within LRK53_RS03695 at 872.051 kb on + strand, within LRK53_RS03695 at 872.051 kb on + strand, within LRK53_RS03695 at 872.051 kb on + strand, within LRK53_RS03695 at 872.051 kb on + strand, within LRK53_RS03695 at 872.051 kb on + strand, within LRK53_RS03695 at 872.051 kb on + strand, within LRK53_RS03695 at 872.051 kb on + strand, within LRK53_RS03695 at 872.052 kb on - strand, within LRK53_RS03695 at 872.052 kb on - strand, within LRK53_RS03695 at 872.052 kb on - strand, within LRK53_RS03695 at 872.052 kb on - strand, within LRK53_RS03695 at 872.225 kb on - strand, within LRK53_RS03695 at 872.225 kb on - strand, within LRK53_RS03695 at 872.276 kb on + strand, within LRK53_RS03695 at 872.276 kb on + strand, within LRK53_RS03695 at 872.276 kb on + strand, within LRK53_RS03695 at 872.276 kb on + strand, within LRK53_RS03695 at 872.276 kb on + strand, within LRK53_RS03695 at 872.277 kb on - strand, within LRK53_RS03695 at 872.277 kb on - strand, within LRK53_RS03695 at 872.277 kb on - strand, within LRK53_RS03695 at 872.277 kb on - strand, within LRK53_RS03695 at 872.277 kb on - strand, within LRK53_RS03695 at 872.277 kb on - strand, within LRK53_RS03695 at 872.277 kb on - strand, within LRK53_RS03695 at 872.277 kb on - strand, within LRK53_RS03695 at 872.277 kb on - strand, within LRK53_RS03695 at 872.358 kb on + strand at 872.358 kb on + strand at 872.358 kb on + strand at 872.358 kb on + strand at 872.359 kb on - strand at 872.359 kb on - strand at 872.359 kb on - strand at 872.359 kb on - strand at 872.359 kb on - strand at 872.449 kb on + strand at 872.449 kb on + strand at 872.449 kb on + strand at 874.194 kb on + strand, within LRK53_RS03700 at 874.508 kb on - strand at 874.508 kb on - strand at 874.508 kb on - strand at 874.508 kb on - strand at 874.508 kb on - strand at 874.508 kb on - strand at 874.508 kb on - strand at 874.508 kb on - strand at 874.529 kb on + strand at 874.530 kb on - strand at 874.530 kb on - strand at 874.530 kb on - strand at 874.530 kb on - strand at 875.024 kb on + strand at 875.144 kb on + strand at 875.144 kb on + strand at 875.144 kb on + strand at 875.144 kb on + strand at 875.144 kb on + strand at 875.145 kb on - strand at 875.145 kb on - strand at 875.145 kb on - strand at 875.145 kb on - strand at 875.145 kb on - strand at 875.145 kb on - strand at 875.290 kb on + strand at 875.290 kb on + strand at 875.291 kb on - strand at 875.291 kb on - strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.303 kb on + strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.304 kb on - strand at 875.317 kb on + strand at 875.317 kb on + strand at 875.317 kb on + strand at 875.317 kb on + strand at 875.317 kb on + strand at 875.317 kb on + strand at 875.317 kb on + strand at 875.317 kb on + strand at 875.317 kb on + strand at 875.317 kb on + strand at 875.317 kb on + strand at 875.317 kb on + strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.318 kb on - strand at 875.328 kb on - strand at 875.328 kb on - strand at 875.332 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 871,626 + LRK53_RS03695 0.42 -2.8 871,626 + LRK53_RS03695 0.42 +0.3 871,626 + LRK53_RS03695 0.42 +0.3 871,626 + LRK53_RS03695 0.42 -2.5 871,626 + LRK53_RS03695 0.42 -1.3 871,626 + LRK53_RS03695 0.42 -1.7 871,626 + LRK53_RS03695 0.42 -1.3 871,626 + LRK53_RS03695 0.42 -0.3 871,626 + LRK53_RS03695 0.42 +0.3 871,627 - LRK53_RS03695 0.42 -1.7 871,627 - LRK53_RS03695 0.42 -1.3 871,627 - LRK53_RS03695 0.42 -0.7 871,627 - LRK53_RS03695 0.42 -1.7 871,627 - LRK53_RS03695 0.42 -1.0 871,627 - LRK53_RS03695 0.42 -1.5 871,627 - LRK53_RS03695 0.42 -0.7 871,627 - LRK53_RS03695 0.42 -1.7 871,627 - LRK53_RS03695 0.42 -1.7 871,627 - LRK53_RS03695 0.42 -2.6 871,647 + LRK53_RS03695 0.43 -2.6 871,647 + LRK53_RS03695 0.43 -0.7 871,647 + LRK53_RS03695 0.43 -1.5 871,647 + LRK53_RS03695 0.43 -0.7 871,647 + LRK53_RS03695 0.43 -1.5 871,647 + LRK53_RS03695 0.43 -1.5 871,647 + LRK53_RS03695 0.43 -1.3 871,647 + LRK53_RS03695 0.43 -1.3 871,647 + LRK53_RS03695 0.43 +1.3 871,647 + LRK53_RS03695 0.43 -0.3 871,647 + LRK53_RS03695 0.43 +0.3 871,647 + LRK53_RS03695 0.43 -0.3 871,647 + LRK53_RS03695 0.43 -1.9 871,647 + LRK53_RS03695 0.43 -1.5 871,648 - LRK53_RS03695 0.44 -0.3 871,648 - LRK53_RS03695 0.44 -1.0 871,648 - LRK53_RS03695 0.44 -1.5 871,648 - LRK53_RS03695 0.44 -2.8 871,648 - LRK53_RS03695 0.44 -0.7 871,648 - LRK53_RS03695 0.44 -1.3 871,648 - LRK53_RS03695 0.44 -2.0 871,648 - LRK53_RS03695 0.44 -2.0 871,648 - LRK53_RS03695 0.44 -1.7 871,648 - LRK53_RS03695 0.44 +0.3 871,648 - LRK53_RS03695 0.44 -2.9 871,648 - LRK53_RS03695 0.44 -1.5 871,648 - LRK53_RS03695 0.44 -1.0 871,648 - LRK53_RS03695 0.44 +0.3 871,648 - LRK53_RS03695 0.44 +0.3 871,656 + LRK53_RS03695 0.44 -0.3 871,657 - LRK53_RS03695 0.44 +0.3 871,657 - LRK53_RS03695 0.44 +1.3 871,752 + LRK53_RS03695 0.51 -0.7 871,752 + LRK53_RS03695 0.51 -0.3 871,752 + LRK53_RS03695 0.51 +1.3 871,752 + LRK53_RS03695 0.51 +0.3 871,752 + LRK53_RS03695 0.51 -3.9 871,753 - LRK53_RS03695 0.51 +0.3 871,753 - LRK53_RS03695 0.51 -0.3 871,753 - LRK53_RS03695 0.51 -0.7 871,753 - LRK53_RS03695 0.51 -0.7 871,753 - LRK53_RS03695 0.51 -1.5 871,753 - LRK53_RS03695 0.51 -2.0 871,935 + LRK53_RS03695 0.65 -1.2 871,935 + LRK53_RS03695 0.65 -2.5 871,935 + LRK53_RS03695 0.65 -1.0 871,935 + LRK53_RS03695 0.65 -3.5 871,935 + LRK53_RS03695 0.65 -2.7 871,935 + LRK53_RS03695 0.65 -2.7 871,935 + LRK53_RS03695 0.65 -3.3 871,935 + LRK53_RS03695 0.65 -2.0 871,935 + LRK53_RS03695 0.65 -2.0 871,935 + LRK53_RS03695 0.65 -1.3 871,935 + LRK53_RS03695 0.65 -1.9 871,935 + LRK53_RS03695 0.65 +0.7 871,935 + LRK53_RS03695 0.65 -2.4 871,935 + LRK53_RS03695 0.65 -0.3 871,935 + LRK53_RS03695 0.65 -1.5 871,935 + LRK53_RS03695 0.65 -0.3 871,935 + LRK53_RS03695 0.65 -1.0 871,935 + LRK53_RS03695 0.65 -0.7 871,936 - LRK53_RS03695 0.65 -5.1 871,936 - LRK53_RS03695 0.65 -1.5 871,936 - LRK53_RS03695 0.65 -3.1 871,936 - LRK53_RS03695 0.65 -1.0 871,936 - LRK53_RS03695 0.65 -2.3 871,936 - LRK53_RS03695 0.65 -1.9 871,936 - LRK53_RS03695 0.65 -1.5 871,936 - LRK53_RS03695 0.65 -1.7 871,936 - LRK53_RS03695 0.65 -1.3 871,936 - LRK53_RS03695 0.65 -0.0 871,936 - LRK53_RS03695 0.65 -2.5 871,936 - LRK53_RS03695 0.65 -0.7 871,936 - LRK53_RS03695 0.65 +0.3 871,936 - LRK53_RS03695 0.65 -0.7 871,936 - LRK53_RS03695 0.65 +1.3 871,936 - LRK53_RS03695 0.65 -0.7 871,953 + LRK53_RS03695 0.66 -1.7 871,953 + LRK53_RS03695 0.66 -1.3 872,051 + LRK53_RS03695 0.73 -1.0 872,051 + LRK53_RS03695 0.73 -2.7 872,051 + LRK53_RS03695 0.73 -0.7 872,051 + LRK53_RS03695 0.73 -2.2 872,051 + LRK53_RS03695 0.73 -1.9 872,051 + LRK53_RS03695 0.73 -1.9 872,051 + LRK53_RS03695 0.73 -1.9 872,052 - LRK53_RS03695 0.73 -1.0 872,052 - LRK53_RS03695 0.73 -4.9 872,052 - LRK53_RS03695 0.73 -2.9 872,052 - LRK53_RS03695 0.73 -1.5 872,225 - LRK53_RS03695 0.86 -0.7 872,225 - LRK53_RS03695 0.86 +0.3 872,276 + LRK53_RS03695 0.89 -0.7 872,276 + LRK53_RS03695 0.89 -1.3 872,276 + LRK53_RS03695 0.89 +0.3 872,276 + LRK53_RS03695 0.89 -0.9 872,276 + LRK53_RS03695 0.89 -1.3 872,277 - LRK53_RS03695 0.90 -2.2 872,277 - LRK53_RS03695 0.90 +0.3 872,277 - LRK53_RS03695 0.90 +1.3 872,277 - LRK53_RS03695 0.90 -1.7 872,277 - LRK53_RS03695 0.90 -1.5 872,277 - LRK53_RS03695 0.90 -0.3 872,277 - LRK53_RS03695 0.90 +0.3 872,277 - LRK53_RS03695 0.90 -0.7 872,277 - LRK53_RS03695 0.90 +1.3 872,358 + -0.3 872,358 + -2.0 872,358 + -3.2 872,358 + -2.5 872,359 - -1.0 872,359 - -0.3 872,359 - -1.0 872,359 - -0.3 872,359 - +0.3 872,449 + -0.9 872,449 + -0.9 872,449 + -0.3 874,194 + LRK53_RS03700 0.88 -2.5 874,508 - -0.7 874,508 - -0.7 874,508 - +0.3 874,508 - -0.6 874,508 - -0.7 874,508 - +2.3 874,508 - -1.3 874,508 - -1.7 874,529 + +1.3 874,530 - +2.3 874,530 - -0.3 874,530 - -1.0 874,530 - -1.0 875,024 + -1.3 875,144 + +0.7 875,144 + +1.9 875,144 + +1.3 875,144 + -1.0 875,144 + +1.3 875,145 - -0.7 875,145 - -1.6 875,145 - -0.0 875,145 - +1.3 875,145 - -1.2 875,145 - -0.5 875,290 + +1.3 875,290 + +0.3 875,291 - +0.9 875,291 - +1.9 875,303 + +0.9 875,303 + -1.0 875,303 + -1.1 875,303 + -0.0 875,303 + -0.5 875,303 + -0.6 875,303 + -0.1 875,303 + -0.8 875,303 + -1.0 875,303 + -0.7 875,303 + -0.9 875,303 + -1.0 875,303 + -1.5 875,303 + -1.6 875,303 + -0.6 875,304 - -2.1 875,304 - -3.7 875,304 - -0.5 875,304 - -0.7 875,304 - +0.1 875,304 - -0.6 875,304 - -1.1 875,304 - -0.1 875,304 - -0.1 875,304 - -0.8 875,304 - -1.2 875,304 - -0.9 875,304 - -1.5 875,304 - -0.1 875,304 - -0.1 875,304 - -0.2 875,304 - -0.9 875,304 - -1.5 875,304 - -0.5 875,304 - +1.6 875,304 - +1.3 875,304 - +0.3 875,304 - -0.3 875,304 - -0.7 875,304 - -1.2 875,304 - -0.6 875,304 - -0.5 875,304 - -1.9 875,304 - -0.3 875,304 - -0.3 875,317 + -0.5 875,317 + -1.3 875,317 + -0.9 875,317 + -0.2 875,317 + -1.0 875,317 + +1.7 875,317 + +0.3 875,317 + -0.4 875,317 + -0.1 875,317 + +0.2 875,317 + -1.1 875,317 + -1.3 875,318 - +0.3 875,318 - +0.3 875,318 - +0.3 875,318 - -0.7 875,318 - -0.8 875,318 - -1.7 875,318 - +0.0 875,318 - -0.2 875,318 - +0.3 875,318 - -0.7 875,318 - -2.7 875,318 - -0.6 875,318 - -0.7 875,318 - +0.0 875,328 - -0.5 875,328 - -0.7 875,332 - -0.5
Or see this region's nucleotide sequence