Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03405 and LRK53_RS03410 overlap by 4 nucleotides LRK53_RS03410 and LRK53_RS03415 are separated by 121 nucleotides LRK53_RS03415 and LRK53_RS03420 are separated by 40 nucleotides
LRK53_RS03405: LRK53_RS03405 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 805,737 to 807,386
_RS03405
LRK53_RS03410: LRK53_RS03410 - SCP2 sterol-binding domain-containing protein, at 807,383 to 808,033
_RS03410
LRK53_RS03415: LRK53_RS03415 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 808,155 to 808,640
_RS03415
LRK53_RS03420: LRK53_RS03420 - hypothetical protein, at 808,681 to 808,884
_RS03420
Position (kb) 807
808
809 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 807.455 kb on + strand, within LRK53_RS03410 at 807.455 kb on + strand, within LRK53_RS03410 at 807.455 kb on + strand, within LRK53_RS03410 at 807.455 kb on + strand, within LRK53_RS03410 at 807.456 kb on - strand, within LRK53_RS03410 at 807.456 kb on - strand, within LRK53_RS03410 at 807.456 kb on - strand, within LRK53_RS03410 at 807.456 kb on - strand, within LRK53_RS03410 at 808.044 kb on + strand at 808.045 kb on - strand at 808.045 kb on - strand at 808.045 kb on - strand at 808.087 kb on - strand at 808.087 kb on - strand at 808.462 kb on + strand, within LRK53_RS03415 at 808.462 kb on + strand, within LRK53_RS03415 at 808.462 kb on + strand, within LRK53_RS03415 at 808.463 kb on - strand, within LRK53_RS03415 at 808.463 kb on - strand, within LRK53_RS03415 at 808.480 kb on + strand, within LRK53_RS03415 at 808.481 kb on - strand, within LRK53_RS03415 at 808.481 kb on - strand, within LRK53_RS03415 at 808.489 kb on + strand, within LRK53_RS03415 at 808.489 kb on + strand, within LRK53_RS03415 at 808.489 kb on + strand, within LRK53_RS03415 at 808.489 kb on + strand, within LRK53_RS03415 at 808.568 kb on + strand, within LRK53_RS03415 at 808.599 kb on - strand at 808.650 kb on - strand at 808.731 kb on + strand, within LRK53_RS03420 at 808.955 kb on + strand at 808.955 kb on + strand at 808.956 kb on - strand at 808.961 kb on + strand at 808.961 kb on + strand at 808.961 kb on + strand at 808.962 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 807,455 + LRK53_RS03410 0.11 -0.3 807,455 + LRK53_RS03410 0.11 -1.4 807,455 + LRK53_RS03410 0.11 -0.7 807,455 + LRK53_RS03410 0.11 -2.1 807,456 - LRK53_RS03410 0.11 -2.5 807,456 - LRK53_RS03410 0.11 +0.3 807,456 - LRK53_RS03410 0.11 -2.5 807,456 - LRK53_RS03410 0.11 -1.9 808,044 + +1.3 808,045 - -0.7 808,045 - +1.3 808,045 - +2.3 808,087 - +0.3 808,087 - -1.7 808,462 + LRK53_RS03415 0.63 +0.3 808,462 + LRK53_RS03415 0.63 +1.3 808,462 + LRK53_RS03415 0.63 -1.0 808,463 - LRK53_RS03415 0.63 +3.3 808,463 - LRK53_RS03415 0.63 -0.0 808,480 + LRK53_RS03415 0.67 -0.8 808,481 - LRK53_RS03415 0.67 -0.1 808,481 - LRK53_RS03415 0.67 -0.0 808,489 + LRK53_RS03415 0.69 +1.3 808,489 + LRK53_RS03415 0.69 -1.1 808,489 + LRK53_RS03415 0.69 -0.3 808,489 + LRK53_RS03415 0.69 -2.4 808,568 + LRK53_RS03415 0.85 +1.3 808,599 - +1.0 808,650 - -2.3 808,731 + LRK53_RS03420 0.25 -1.0 808,955 + -0.8 808,955 + -0.1 808,956 - -0.7 808,961 + +0.0 808,961 + +0.3 808,961 + -0.8 808,962 - +0.7
Or see this region's nucleotide sequence