Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02535 and LRK53_RS02540 are separated by 60 nucleotides LRK53_RS02540 and LRK53_RS02545 are separated by 149 nucleotides LRK53_RS02545 and LRK53_RS02550 are separated by 168 nucleotides LRK53_RS02550 and LRK53_RS02555 are separated by 39 nucleotides
LRK53_RS02535: LRK53_RS02535 - DUF481 domain-containing protein, at 578,763 to 579,572
_RS02535
LRK53_RS02540: LRK53_RS02540 - hypothetical protein, at 579,633 to 580,304
_RS02540
LRK53_RS02545: LRK53_RS02545 - 2,3-diphosphoglycerate-dependent phosphoglycerate mutase, at 580,454 to 581,197
_RS02545
LRK53_RS02550: LRK53_RS02550 - hypothetical protein, at 581,366 to 581,914
_RS02550
LRK53_RS02555: LRK53_RS02555 - amidohydrolase, at 581,954 to 583,258
_RS02555
Position (kb) 580
581
582 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 579.493 kb on + strand at 579.494 kb on - strand at 579.494 kb on - strand at 579.494 kb on - strand at 579.494 kb on - strand at 579.561 kb on + strand at 579.561 kb on + strand at 579.561 kb on + strand at 579.561 kb on + strand at 579.562 kb on - strand at 579.562 kb on - strand at 579.562 kb on - strand at 579.562 kb on - strand at 579.562 kb on - strand at 579.562 kb on - strand at 579.570 kb on + strand at 579.570 kb on + strand at 579.570 kb on + strand at 579.570 kb on + strand at 579.570 kb on + strand at 579.570 kb on + strand at 579.571 kb on - strand at 579.571 kb on - strand at 579.571 kb on - strand at 579.571 kb on - strand at 579.571 kb on - strand at 579.617 kb on + strand at 579.617 kb on + strand at 579.634 kb on + strand at 579.634 kb on + strand at 579.635 kb on - strand at 579.635 kb on - strand at 580.136 kb on + strand, within LRK53_RS02540 at 580.310 kb on + strand at 580.335 kb on + strand at 580.336 kb on - strand at 580.336 kb on - strand at 580.336 kb on - strand at 580.336 kb on - strand at 580.596 kb on - strand, within LRK53_RS02545 at 580.766 kb on + strand, within LRK53_RS02545 at 580.767 kb on - strand, within LRK53_RS02545 at 580.802 kb on + strand, within LRK53_RS02545 at 580.802 kb on + strand, within LRK53_RS02545 at 580.803 kb on - strand, within LRK53_RS02545 at 580.934 kb on + strand, within LRK53_RS02545 at 581.024 kb on + strand, within LRK53_RS02545 at 581.024 kb on + strand, within LRK53_RS02545 at 581.025 kb on - strand, within LRK53_RS02545 at 581.025 kb on - strand, within LRK53_RS02545 at 581.090 kb on + strand, within LRK53_RS02545 at 581.091 kb on - strand, within LRK53_RS02545 at 581.126 kb on + strand at 581.126 kb on + strand at 581.127 kb on - strand at 581.127 kb on - strand at 581.127 kb on - strand at 581.127 kb on - strand at 581.129 kb on + strand at 581.129 kb on + strand at 581.129 kb on + strand at 581.129 kb on + strand at 581.130 kb on - strand at 581.130 kb on - strand at 581.130 kb on - strand at 581.130 kb on - strand at 581.130 kb on - strand at 581.130 kb on - strand at 581.195 kb on + strand at 581.195 kb on + strand at 581.195 kb on + strand at 581.196 kb on - strand at 581.196 kb on - strand at 581.196 kb on - strand at 581.259 kb on + strand at 581.259 kb on + strand at 581.259 kb on + strand at 581.259 kb on + strand at 581.259 kb on + strand at 581.260 kb on - strand at 581.260 kb on - strand at 581.260 kb on - strand at 581.332 kb on - strand at 581.332 kb on - strand at 581.387 kb on + strand at 581.387 kb on + strand at 581.387 kb on + strand at 581.387 kb on + strand at 581.387 kb on + strand at 581.387 kb on + strand at 581.387 kb on + strand at 581.387 kb on + strand at 581.387 kb on + strand at 581.387 kb on + strand at 581.387 kb on + strand at 581.388 kb on - strand at 581.388 kb on - strand at 581.388 kb on - strand at 581.388 kb on - strand at 581.388 kb on - strand at 581.388 kb on - strand at 581.388 kb on - strand at 581.388 kb on - strand at 581.388 kb on - strand at 581.388 kb on - strand at 581.388 kb on - strand at 581.421 kb on + strand, within LRK53_RS02550 at 581.421 kb on + strand, within LRK53_RS02550 at 581.421 kb on + strand, within LRK53_RS02550 at 581.421 kb on + strand, within LRK53_RS02550 at 581.421 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.648 kb on + strand, within LRK53_RS02550 at 581.649 kb on - strand, within LRK53_RS02550 at 581.649 kb on - strand, within LRK53_RS02550 at 581.649 kb on - strand, within LRK53_RS02550 at 581.649 kb on - strand, within LRK53_RS02550 at 581.649 kb on - strand, within LRK53_RS02550 at 581.649 kb on - strand, within LRK53_RS02550 at 581.649 kb on - strand, within LRK53_RS02550 at 581.783 kb on + strand, within LRK53_RS02550 at 581.783 kb on + strand, within LRK53_RS02550 at 581.783 kb on + strand, within LRK53_RS02550 at 581.831 kb on + strand, within LRK53_RS02550 at 581.831 kb on + strand, within LRK53_RS02550 at 581.831 kb on + strand, within LRK53_RS02550 at 581.831 kb on + strand, within LRK53_RS02550 at 581.832 kb on - strand, within LRK53_RS02550 at 581.832 kb on - strand, within LRK53_RS02550 at 581.832 kb on - strand, within LRK53_RS02550 at 581.832 kb on - strand, within LRK53_RS02550 at 581.832 kb on - strand, within LRK53_RS02550 at 581.832 kb on - strand, within LRK53_RS02550 at 581.832 kb on - strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.834 kb on + strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.835 kb on - strand, within LRK53_RS02550 at 581.843 kb on + strand, within LRK53_RS02550 at 581.843 kb on + strand, within LRK53_RS02550 at 581.843 kb on + strand, within LRK53_RS02550 at 581.843 kb on + strand, within LRK53_RS02550 at 581.843 kb on + strand, within LRK53_RS02550 at 581.844 kb on - strand, within LRK53_RS02550 at 581.844 kb on - strand, within LRK53_RS02550 at 581.844 kb on - strand, within LRK53_RS02550 at 581.912 kb on + strand at 581.912 kb on + strand at 581.913 kb on - strand at 581.913 kb on - strand at 581.913 kb on - strand at 581.913 kb on - strand at 581.976 kb on + strand at 581.976 kb on + strand at 581.976 kb on + strand at 581.976 kb on + strand at 581.976 kb on + strand at 581.977 kb on - strand at 581.977 kb on - strand at 582.108 kb on + strand, within LRK53_RS02555 at 582.108 kb on + strand, within LRK53_RS02555 at 582.108 kb on + strand, within LRK53_RS02555 at 582.108 kb on + strand, within LRK53_RS02555 at 582.109 kb on - strand, within LRK53_RS02555 at 582.109 kb on - strand, within LRK53_RS02555 at 582.109 kb on - strand, within LRK53_RS02555 at 582.109 kb on - strand, within LRK53_RS02555 at 582.109 kb on - strand, within LRK53_RS02555 at 582.109 kb on - strand, within LRK53_RS02555 at 582.135 kb on + strand, within LRK53_RS02555 at 582.135 kb on + strand, within LRK53_RS02555 at 582.135 kb on + strand, within LRK53_RS02555 at 582.135 kb on + strand, within LRK53_RS02555 at 582.136 kb on - strand, within LRK53_RS02555 at 582.136 kb on - strand, within LRK53_RS02555 at 582.136 kb on - strand, within LRK53_RS02555
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 579,493 + -1.5 579,494 - +0.1 579,494 - +0.3 579,494 - -0.7 579,494 - +0.7 579,561 + +1.3 579,561 + -0.5 579,561 + +1.3 579,561 + +0.3 579,562 - +0.3 579,562 - +1.3 579,562 - -2.0 579,562 - -0.6 579,562 - +2.3 579,562 - +0.3 579,570 + -0.7 579,570 + +0.2 579,570 + -0.7 579,570 + +1.9 579,570 + -0.1 579,570 + +0.3 579,571 - +0.3 579,571 - -1.0 579,571 - -0.8 579,571 - -1.7 579,571 - +1.3 579,617 + -1.8 579,617 + +0.3 579,634 + -1.0 579,634 + -1.1 579,635 - -1.0 579,635 - +1.9 580,136 + LRK53_RS02540 0.75 -1.0 580,310 + -0.8 580,335 + -0.3 580,336 - -0.3 580,336 - -0.3 580,336 - -1.0 580,336 - +0.7 580,596 - LRK53_RS02545 0.19 -0.7 580,766 + LRK53_RS02545 0.42 +1.3 580,767 - LRK53_RS02545 0.42 -0.3 580,802 + LRK53_RS02545 0.47 +1.3 580,802 + LRK53_RS02545 0.47 -1.0 580,803 - LRK53_RS02545 0.47 -1.6 580,934 + LRK53_RS02545 0.65 -1.0 581,024 + LRK53_RS02545 0.77 -1.0 581,024 + LRK53_RS02545 0.77 +0.3 581,025 - LRK53_RS02545 0.77 -0.3 581,025 - LRK53_RS02545 0.77 -1.0 581,090 + LRK53_RS02545 0.85 -0.3 581,091 - LRK53_RS02545 0.86 +1.3 581,126 + +0.0 581,126 + -0.0 581,127 - +0.5 581,127 - +0.3 581,127 - +0.0 581,127 - -0.3 581,129 + +0.3 581,129 + +0.0 581,129 + -2.1 581,129 + -0.3 581,130 - -0.3 581,130 - +1.3 581,130 - +0.3 581,130 - -0.7 581,130 - -1.8 581,130 - +0.3 581,195 + -1.5 581,195 + -0.3 581,195 + +0.3 581,196 - -1.6 581,196 - +1.3 581,196 - +0.9 581,259 + -0.3 581,259 + +0.2 581,259 + +1.3 581,259 + -1.0 581,259 + -0.7 581,260 - +0.5 581,260 - -1.4 581,260 - +1.3 581,332 - +0.2 581,332 - +0.0 581,387 + +0.3 581,387 + +0.7 581,387 + +0.7 581,387 + +1.1 581,387 + +2.9 581,387 + -0.3 581,387 + +0.3 581,387 + -1.0 581,387 + +1.9 581,387 + +0.3 581,387 + -0.7 581,388 - -2.0 581,388 - +0.3 581,388 - +0.0 581,388 - -2.1 581,388 - +0.7 581,388 - -0.1 581,388 - -0.3 581,388 - -0.1 581,388 - +2.6 581,388 - +0.3 581,388 - +0.3 581,421 + LRK53_RS02550 0.10 -1.2 581,421 + LRK53_RS02550 0.10 +0.7 581,421 + LRK53_RS02550 0.10 -1.7 581,421 + LRK53_RS02550 0.10 -0.7 581,421 + LRK53_RS02550 0.10 +0.1 581,648 + LRK53_RS02550 0.51 -1.5 581,648 + LRK53_RS02550 0.51 -0.5 581,648 + LRK53_RS02550 0.51 -0.5 581,648 + LRK53_RS02550 0.51 -0.3 581,648 + LRK53_RS02550 0.51 +0.1 581,648 + LRK53_RS02550 0.51 -0.3 581,648 + LRK53_RS02550 0.51 +2.9 581,648 + LRK53_RS02550 0.51 -1.5 581,648 + LRK53_RS02550 0.51 -1.8 581,648 + LRK53_RS02550 0.51 -0.3 581,648 + LRK53_RS02550 0.51 +0.3 581,648 + LRK53_RS02550 0.51 +0.3 581,648 + LRK53_RS02550 0.51 -0.3 581,648 + LRK53_RS02550 0.51 +0.3 581,649 - LRK53_RS02550 0.52 -2.8 581,649 - LRK53_RS02550 0.52 +0.7 581,649 - LRK53_RS02550 0.52 +1.3 581,649 - LRK53_RS02550 0.52 +0.7 581,649 - LRK53_RS02550 0.52 +0.3 581,649 - LRK53_RS02550 0.52 +0.5 581,649 - LRK53_RS02550 0.52 +0.3 581,783 + LRK53_RS02550 0.76 +0.8 581,783 + LRK53_RS02550 0.76 -1.4 581,783 + LRK53_RS02550 0.76 +1.3 581,831 + LRK53_RS02550 0.85 +0.3 581,831 + LRK53_RS02550 0.85 -1.0 581,831 + LRK53_RS02550 0.85 -0.0 581,831 + LRK53_RS02550 0.85 -0.3 581,832 - LRK53_RS02550 0.85 +0.3 581,832 - LRK53_RS02550 0.85 -0.4 581,832 - LRK53_RS02550 0.85 +1.1 581,832 - LRK53_RS02550 0.85 +1.3 581,832 - LRK53_RS02550 0.85 -0.8 581,832 - LRK53_RS02550 0.85 -0.7 581,832 - LRK53_RS02550 0.85 -1.0 581,834 + LRK53_RS02550 0.85 -1.7 581,834 + LRK53_RS02550 0.85 +0.3 581,834 + LRK53_RS02550 0.85 +1.0 581,834 + LRK53_RS02550 0.85 -0.2 581,834 + LRK53_RS02550 0.85 +0.1 581,834 + LRK53_RS02550 0.85 -1.3 581,834 + LRK53_RS02550 0.85 -1.0 581,834 + LRK53_RS02550 0.85 +0.1 581,834 + LRK53_RS02550 0.85 -0.1 581,834 + LRK53_RS02550 0.85 +0.0 581,834 + LRK53_RS02550 0.85 -0.4 581,834 + LRK53_RS02550 0.85 -1.2 581,834 + LRK53_RS02550 0.85 -0.3 581,834 + LRK53_RS02550 0.85 -0.5 581,834 + LRK53_RS02550 0.85 +1.7 581,834 + LRK53_RS02550 0.85 -0.4 581,834 + LRK53_RS02550 0.85 -0.7 581,834 + LRK53_RS02550 0.85 +0.7 581,834 + LRK53_RS02550 0.85 -1.5 581,834 + LRK53_RS02550 0.85 +1.3 581,834 + LRK53_RS02550 0.85 -2.1 581,834 + LRK53_RS02550 0.85 -1.5 581,834 + LRK53_RS02550 0.85 -1.3 581,834 + LRK53_RS02550 0.85 -0.2 581,835 - LRK53_RS02550 0.85 +0.3 581,835 - LRK53_RS02550 0.85 -1.0 581,835 - LRK53_RS02550 0.85 +0.7 581,835 - LRK53_RS02550 0.85 -0.8 581,835 - LRK53_RS02550 0.85 +0.0 581,835 - LRK53_RS02550 0.85 -0.7 581,835 - LRK53_RS02550 0.85 +0.3 581,835 - LRK53_RS02550 0.85 -1.0 581,835 - LRK53_RS02550 0.85 -0.8 581,835 - LRK53_RS02550 0.85 -0.5 581,835 - LRK53_RS02550 0.85 -1.5 581,835 - LRK53_RS02550 0.85 +1.3 581,835 - LRK53_RS02550 0.85 -0.6 581,835 - LRK53_RS02550 0.85 +1.9 581,835 - LRK53_RS02550 0.85 +0.7 581,835 - LRK53_RS02550 0.85 -1.3 581,835 - LRK53_RS02550 0.85 +0.9 581,835 - LRK53_RS02550 0.85 -0.3 581,835 - LRK53_RS02550 0.85 +1.7 581,835 - LRK53_RS02550 0.85 -1.8 581,835 - LRK53_RS02550 0.85 +0.3 581,835 - LRK53_RS02550 0.85 +0.6 581,835 - LRK53_RS02550 0.85 -0.3 581,835 - LRK53_RS02550 0.85 -0.7 581,835 - LRK53_RS02550 0.85 +0.5 581,835 - LRK53_RS02550 0.85 -0.3 581,835 - LRK53_RS02550 0.85 -1.0 581,835 - LRK53_RS02550 0.85 -0.7 581,835 - LRK53_RS02550 0.85 +1.7 581,843 + LRK53_RS02550 0.87 -1.8 581,843 + LRK53_RS02550 0.87 -0.5 581,843 + LRK53_RS02550 0.87 -1.0 581,843 + LRK53_RS02550 0.87 -0.3 581,843 + LRK53_RS02550 0.87 -0.5 581,844 - LRK53_RS02550 0.87 -0.3 581,844 - LRK53_RS02550 0.87 +0.0 581,844 - LRK53_RS02550 0.87 -0.3 581,912 + +0.3 581,912 + +0.0 581,913 - +1.3 581,913 - +0.2 581,913 - -2.0 581,913 - -0.7 581,976 + +2.3 581,976 + -0.4 581,976 + +0.3 581,976 + -1.2 581,976 + +0.7 581,977 - +0.0 581,977 - +0.3 582,108 + LRK53_RS02555 0.12 -0.6 582,108 + LRK53_RS02555 0.12 +1.9 582,108 + LRK53_RS02555 0.12 -0.3 582,108 + LRK53_RS02555 0.12 -0.7 582,109 - LRK53_RS02555 0.12 +0.0 582,109 - LRK53_RS02555 0.12 -0.8 582,109 - LRK53_RS02555 0.12 -1.2 582,109 - LRK53_RS02555 0.12 -0.3 582,109 - LRK53_RS02555 0.12 -0.1 582,109 - LRK53_RS02555 0.12 +1.3 582,135 + LRK53_RS02555 0.14 -0.6 582,135 + LRK53_RS02555 0.14 -0.5 582,135 + LRK53_RS02555 0.14 +0.3 582,135 + LRK53_RS02555 0.14 -0.3 582,136 - LRK53_RS02555 0.14 +1.9 582,136 - LRK53_RS02555 0.14 +0.0 582,136 - LRK53_RS02555 0.14 +0.3
Or see this region's nucleotide sequence