Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02275 and LRK53_RS02280 are separated by 68 nucleotides LRK53_RS02280 and LRK53_RS02285 overlap by 4 nucleotides LRK53_RS02285 and LRK53_RS02290 are separated by 160 nucleotides
LRK53_RS02275: LRK53_RS02275 - YdcF family protein, at 526,850 to 527,587
_RS02275
LRK53_RS02280: LRK53_RS02280 - hypothetical protein, at 527,656 to 528,168
_RS02280
LRK53_RS02285: LRK53_RS02285 - oxygen-dependent coproporphyrinogen oxidase, at 528,165 to 529,067
_RS02285
LRK53_RS02290: LRK53_RS02290 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE, at 529,228 to 530,571
_RS02290
Position (kb) 528
529
530 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 527.481 kb on - strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.511 kb on - strand, within LRK53_RS02275 at 527.511 kb on - strand, within LRK53_RS02275 at 527.511 kb on - strand, within LRK53_RS02275 at 527.511 kb on - strand, within LRK53_RS02275 at 527.763 kb on + strand, within LRK53_RS02280 at 527.763 kb on + strand, within LRK53_RS02280 at 527.763 kb on + strand, within LRK53_RS02280 at 527.764 kb on - strand, within LRK53_RS02280 at 527.764 kb on - strand, within LRK53_RS02280 at 527.824 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.875 kb on + strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.876 kb on - strand, within LRK53_RS02280 at 527.899 kb on + strand, within LRK53_RS02280 at 527.899 kb on + strand, within LRK53_RS02280 at 527.899 kb on + strand, within LRK53_RS02280 at 527.899 kb on + strand, within LRK53_RS02280 at 527.899 kb on + strand, within LRK53_RS02280 at 527.899 kb on + strand, within LRK53_RS02280 at 527.899 kb on + strand, within LRK53_RS02280 at 527.899 kb on + strand, within LRK53_RS02280 at 527.899 kb on + strand, within LRK53_RS02280 at 527.899 kb on + strand, within LRK53_RS02280 at 527.900 kb on - strand, within LRK53_RS02280 at 527.900 kb on - strand, within LRK53_RS02280 at 527.900 kb on - strand, within LRK53_RS02280 at 527.900 kb on - strand, within LRK53_RS02280 at 527.900 kb on - strand, within LRK53_RS02280 at 527.900 kb on - strand, within LRK53_RS02280 at 527.900 kb on - strand, within LRK53_RS02280 at 527.900 kb on - strand, within LRK53_RS02280 at 527.900 kb on - strand, within LRK53_RS02280 at 527.900 kb on - strand, within LRK53_RS02280 at 527.941 kb on + strand, within LRK53_RS02280 at 527.942 kb on - strand, within LRK53_RS02280 at 527.942 kb on - strand, within LRK53_RS02280 at 527.942 kb on - strand, within LRK53_RS02280 at 527.942 kb on - strand, within LRK53_RS02280 at 528.131 kb on + strand at 528.131 kb on + strand at 528.131 kb on + strand at 528.131 kb on + strand at 528.131 kb on + strand at 528.132 kb on - strand at 528.132 kb on - strand at 528.132 kb on - strand at 528.132 kb on - strand at 528.132 kb on - strand at 528.589 kb on + strand, within LRK53_RS02285 at 528.589 kb on + strand, within LRK53_RS02285 at 528.675 kb on + strand, within LRK53_RS02285 at 528.675 kb on + strand, within LRK53_RS02285 at 528.675 kb on + strand, within LRK53_RS02285 at 528.681 kb on + strand, within LRK53_RS02285 at 528.726 kb on + strand, within LRK53_RS02285 at 528.726 kb on + strand, within LRK53_RS02285 at 528.726 kb on + strand, within LRK53_RS02285 at 528.804 kb on + strand, within LRK53_RS02285 at 528.805 kb on - strand, within LRK53_RS02285 at 528.840 kb on + strand, within LRK53_RS02285 at 528.886 kb on - strand, within LRK53_RS02285 at 528.907 kb on - strand, within LRK53_RS02285 at 529.035 kb on - strand at 529.035 kb on - strand at 529.041 kb on + strand at 529.041 kb on + strand at 529.042 kb on - strand at 529.042 kb on - strand at 529.042 kb on - strand at 529.066 kb on - strand at 529.066 kb on - strand at 529.066 kb on - strand at 529.066 kb on - strand at 529.066 kb on - strand at 529.066 kb on - strand at 529.185 kb on + strand at 529.185 kb on + strand at 529.185 kb on + strand at 529.447 kb on + strand, within LRK53_RS02290 at 529.447 kb on + strand, within LRK53_RS02290 at 529.447 kb on + strand, within LRK53_RS02290 at 529.447 kb on + strand, within LRK53_RS02290 at 529.448 kb on - strand, within LRK53_RS02290 at 529.448 kb on - strand, within LRK53_RS02290 at 529.734 kb on + strand, within LRK53_RS02290 at 529.735 kb on - strand, within LRK53_RS02290 at 529.859 kb on + strand, within LRK53_RS02290 at 529.977 kb on + strand, within LRK53_RS02290
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 527,481 - LRK53_RS02275 0.86 +0.3 527,510 + LRK53_RS02275 0.89 -1.5 527,510 + LRK53_RS02275 0.89 +0.1 527,510 + LRK53_RS02275 0.89 +0.8 527,510 + LRK53_RS02275 0.89 -0.7 527,510 + LRK53_RS02275 0.89 +0.6 527,510 + LRK53_RS02275 0.89 +1.3 527,511 - LRK53_RS02275 0.90 +0.1 527,511 - LRK53_RS02275 0.90 +0.2 527,511 - LRK53_RS02275 0.90 -1.2 527,511 - LRK53_RS02275 0.90 -0.1 527,763 + LRK53_RS02280 0.21 -0.8 527,763 + LRK53_RS02280 0.21 -0.8 527,763 + LRK53_RS02280 0.21 +0.8 527,764 - LRK53_RS02280 0.21 +0.3 527,764 - LRK53_RS02280 0.21 +0.3 527,824 + LRK53_RS02280 0.33 +1.3 527,875 + LRK53_RS02280 0.43 -0.2 527,875 + LRK53_RS02280 0.43 +1.8 527,875 + LRK53_RS02280 0.43 +1.3 527,875 + LRK53_RS02280 0.43 +1.8 527,875 + LRK53_RS02280 0.43 -0.1 527,875 + LRK53_RS02280 0.43 -0.8 527,875 + LRK53_RS02280 0.43 -0.2 527,875 + LRK53_RS02280 0.43 -0.5 527,875 + LRK53_RS02280 0.43 +1.3 527,875 + LRK53_RS02280 0.43 -0.2 527,875 + LRK53_RS02280 0.43 +1.3 527,875 + LRK53_RS02280 0.43 -2.6 527,875 + LRK53_RS02280 0.43 +1.3 527,875 + LRK53_RS02280 0.43 -0.7 527,875 + LRK53_RS02280 0.43 +0.3 527,875 + LRK53_RS02280 0.43 +1.3 527,875 + LRK53_RS02280 0.43 +2.3 527,875 + LRK53_RS02280 0.43 -0.5 527,875 + LRK53_RS02280 0.43 -0.2 527,875 + LRK53_RS02280 0.43 +0.3 527,875 + LRK53_RS02280 0.43 -0.2 527,875 + LRK53_RS02280 0.43 -0.4 527,875 + LRK53_RS02280 0.43 -0.9 527,876 - LRK53_RS02280 0.43 +0.3 527,876 - LRK53_RS02280 0.43 +0.8 527,876 - LRK53_RS02280 0.43 +1.3 527,876 - LRK53_RS02280 0.43 +0.3 527,876 - LRK53_RS02280 0.43 -0.8 527,876 - LRK53_RS02280 0.43 +0.0 527,876 - LRK53_RS02280 0.43 -0.7 527,876 - LRK53_RS02280 0.43 -0.7 527,876 - LRK53_RS02280 0.43 +0.4 527,876 - LRK53_RS02280 0.43 -0.7 527,876 - LRK53_RS02280 0.43 -0.2 527,876 - LRK53_RS02280 0.43 -1.2 527,876 - LRK53_RS02280 0.43 -0.2 527,876 - LRK53_RS02280 0.43 +0.0 527,876 - LRK53_RS02280 0.43 +0.8 527,876 - LRK53_RS02280 0.43 +0.7 527,876 - LRK53_RS02280 0.43 +0.0 527,876 - LRK53_RS02280 0.43 +1.6 527,876 - LRK53_RS02280 0.43 +2.9 527,876 - LRK53_RS02280 0.43 -0.2 527,876 - LRK53_RS02280 0.43 -1.2 527,876 - LRK53_RS02280 0.43 +0.8 527,899 + LRK53_RS02280 0.47 +0.3 527,899 + LRK53_RS02280 0.47 +0.3 527,899 + LRK53_RS02280 0.47 -1.2 527,899 + LRK53_RS02280 0.47 -0.7 527,899 + LRK53_RS02280 0.47 +0.5 527,899 + LRK53_RS02280 0.47 -0.1 527,899 + LRK53_RS02280 0.47 -0.3 527,899 + LRK53_RS02280 0.47 -0.2 527,899 + LRK53_RS02280 0.47 +0.3 527,899 + LRK53_RS02280 0.47 -1.0 527,900 - LRK53_RS02280 0.48 +1.3 527,900 - LRK53_RS02280 0.48 -0.2 527,900 - LRK53_RS02280 0.48 +0.8 527,900 - LRK53_RS02280 0.48 +0.0 527,900 - LRK53_RS02280 0.48 +0.3 527,900 - LRK53_RS02280 0.48 -0.2 527,900 - LRK53_RS02280 0.48 -0.7 527,900 - LRK53_RS02280 0.48 -0.2 527,900 - LRK53_RS02280 0.48 +2.3 527,900 - LRK53_RS02280 0.48 -1.0 527,941 + LRK53_RS02280 0.56 -0.8 527,942 - LRK53_RS02280 0.56 +0.3 527,942 - LRK53_RS02280 0.56 +0.3 527,942 - LRK53_RS02280 0.56 -0.8 527,942 - LRK53_RS02280 0.56 +0.6 528,131 + -0.7 528,131 + -1.0 528,131 + +0.8 528,131 + +0.3 528,131 + -0.2 528,132 - -1.5 528,132 - +0.3 528,132 - -0.7 528,132 - +2.1 528,132 - -1.5 528,589 + LRK53_RS02285 0.47 +0.3 528,589 + LRK53_RS02285 0.47 +0.3 528,675 + LRK53_RS02285 0.56 +1.3 528,675 + LRK53_RS02285 0.56 +1.3 528,675 + LRK53_RS02285 0.56 +1.3 528,681 + LRK53_RS02285 0.57 +0.3 528,726 + LRK53_RS02285 0.62 +1.3 528,726 + LRK53_RS02285 0.62 -1.0 528,726 + LRK53_RS02285 0.62 +0.3 528,804 + LRK53_RS02285 0.71 -1.0 528,805 - LRK53_RS02285 0.71 -0.2 528,840 + LRK53_RS02285 0.75 -1.0 528,886 - LRK53_RS02285 0.80 +0.3 528,907 - LRK53_RS02285 0.82 -0.7 529,035 - +0.3 529,035 - -2.5 529,041 + -1.0 529,041 + -1.0 529,042 - -1.0 529,042 - -0.5 529,042 - -0.9 529,066 - +0.9 529,066 - -0.7 529,066 - -1.2 529,066 - +0.3 529,066 - -0.7 529,066 - +0.1 529,185 + +0.8 529,185 + -0.2 529,185 + -1.5 529,447 + LRK53_RS02290 0.16 -1.4 529,447 + LRK53_RS02290 0.16 +0.3 529,447 + LRK53_RS02290 0.16 -2.1 529,447 + LRK53_RS02290 0.16 +0.3 529,448 - LRK53_RS02290 0.16 -1.0 529,448 - LRK53_RS02290 0.16 +1.3 529,734 + LRK53_RS02290 0.38 +0.9 529,735 - LRK53_RS02290 0.38 +1.3 529,859 + LRK53_RS02290 0.47 +0.3 529,977 + LRK53_RS02290 0.56 +0.8
Or see this region's nucleotide sequence