Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS01225 and LRK53_RS01230 are separated by 20 nucleotides LRK53_RS01230 and LRK53_RS01235 are separated by 288 nucleotides LRK53_RS01235 and LRK53_RS01240 are separated by 219 nucleotides
LRK53_RS01225: LRK53_RS01225 - ATP-binding protein, at 300,970 to 301,800
_RS01225
LRK53_RS01230: LRK53_RS01230 - hypothetical protein, at 301,821 to 302,126
_RS01230
LRK53_RS01235: LRK53_RS01235 - monovalent cation:proton antiporter-2 (CPA2) family protein, at 302,415 to 304,250
_RS01235
LRK53_RS01240: LRK53_RS01240 - ankyrin repeat domain-containing protein, at 304,470 to 307,910
_RS01240
Position (kb)
302
303
304
305 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 301.444 kb on + strand, within LRK53_RS01225 at 301.444 kb on + strand, within LRK53_RS01225 at 301.445 kb on - strand, within LRK53_RS01225 at 301.445 kb on - strand, within LRK53_RS01225 at 301.445 kb on - strand, within LRK53_RS01225 at 301.445 kb on - strand, within LRK53_RS01225 at 301.445 kb on - strand, within LRK53_RS01225 at 301.445 kb on - strand, within LRK53_RS01225 at 301.447 kb on + strand, within LRK53_RS01225 at 301.447 kb on + strand, within LRK53_RS01225 at 301.447 kb on + strand, within LRK53_RS01225 at 301.447 kb on + strand, within LRK53_RS01225 at 301.447 kb on + strand, within LRK53_RS01225 at 301.447 kb on + strand, within LRK53_RS01225 at 301.447 kb on + strand, within LRK53_RS01225 at 301.447 kb on + strand, within LRK53_RS01225 at 301.447 kb on + strand, within LRK53_RS01225 at 301.447 kb on + strand, within LRK53_RS01225 at 301.448 kb on - strand, within LRK53_RS01225 at 301.448 kb on - strand, within LRK53_RS01225 at 301.448 kb on - strand, within LRK53_RS01225 at 301.448 kb on - strand, within LRK53_RS01225 at 301.448 kb on - strand, within LRK53_RS01225 at 301.448 kb on - strand, within LRK53_RS01225 at 301.448 kb on - strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.579 kb on + strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.580 kb on - strand, within LRK53_RS01225 at 301.616 kb on + strand, within LRK53_RS01225 at 301.616 kb on + strand, within LRK53_RS01225 at 301.616 kb on + strand, within LRK53_RS01225 at 301.616 kb on + strand, within LRK53_RS01225 at 301.616 kb on + strand, within LRK53_RS01225 at 301.617 kb on - strand, within LRK53_RS01225 at 301.712 kb on + strand, within LRK53_RS01225 at 301.712 kb on + strand, within LRK53_RS01225 at 301.712 kb on + strand, within LRK53_RS01225 at 301.712 kb on + strand, within LRK53_RS01225 at 301.712 kb on + strand, within LRK53_RS01225 at 301.712 kb on + strand, within LRK53_RS01225 at 301.712 kb on + strand, within LRK53_RS01225 at 301.713 kb on - strand, within LRK53_RS01225 at 301.713 kb on - strand, within LRK53_RS01225 at 301.713 kb on - strand, within LRK53_RS01225 at 301.713 kb on - strand, within LRK53_RS01225 at 301.713 kb on - strand, within LRK53_RS01225 at 301.713 kb on - strand, within LRK53_RS01225 at 301.738 kb on - strand at 301.759 kb on + strand at 301.759 kb on + strand at 301.759 kb on + strand at 301.759 kb on + strand at 301.760 kb on - strand at 301.760 kb on - strand at 301.760 kb on - strand at 301.760 kb on - strand at 301.760 kb on - strand at 301.760 kb on - strand at 301.851 kb on + strand at 301.852 kb on - strand, within LRK53_RS01230 at 301.852 kb on - strand, within LRK53_RS01230 at 301.966 kb on + strand, within LRK53_RS01230 at 301.966 kb on + strand, within LRK53_RS01230 at 301.967 kb on - strand, within LRK53_RS01230 at 301.967 kb on - strand, within LRK53_RS01230 at 301.967 kb on - strand, within LRK53_RS01230 at 302.083 kb on - strand, within LRK53_RS01230 at 302.169 kb on - strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.170 kb on + strand at 302.171 kb on - strand at 302.171 kb on - strand at 302.171 kb on - strand at 302.171 kb on - strand at 302.171 kb on - strand at 302.171 kb on - strand at 302.171 kb on - strand at 302.171 kb on - strand at 302.171 kb on - strand at 302.171 kb on - strand at 302.192 kb on + strand at 302.192 kb on + strand at 302.192 kb on + strand at 302.192 kb on + strand at 302.192 kb on + strand at 302.193 kb on - strand at 302.193 kb on - strand at 302.193 kb on - strand at 302.193 kb on - strand at 302.193 kb on - strand at 302.193 kb on - strand at 302.241 kb on + strand at 302.241 kb on + strand at 302.241 kb on + strand at 302.242 kb on - strand at 302.242 kb on - strand at 302.283 kb on + strand at 302.283 kb on + strand at 302.284 kb on - strand at 302.284 kb on - strand at 302.311 kb on - strand at 302.733 kb on + strand, within LRK53_RS01235 at 302.733 kb on + strand, within LRK53_RS01235 at 302.733 kb on + strand, within LRK53_RS01235 at 302.734 kb on - strand, within LRK53_RS01235 at 302.734 kb on - strand, within LRK53_RS01235 at 302.995 kb on + strand, within LRK53_RS01235 at 304.069 kb on - strand at 304.104 kb on + strand at 304.105 kb on - strand at 304.248 kb on + strand at 304.312 kb on + strand at 304.312 kb on + strand at 304.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 301,444 + LRK53_RS01225 0.57 +1.4 301,444 + LRK53_RS01225 0.57 +1.4 301,445 - LRK53_RS01225 0.57 +1.4 301,445 - LRK53_RS01225 0.57 -0.6 301,445 - LRK53_RS01225 0.57 -1.4 301,445 - LRK53_RS01225 0.57 +0.2 301,445 - LRK53_RS01225 0.57 -2.2 301,445 - LRK53_RS01225 0.57 -0.2 301,447 + LRK53_RS01225 0.57 -0.9 301,447 + LRK53_RS01225 0.57 +0.2 301,447 + LRK53_RS01225 0.57 -0.8 301,447 + LRK53_RS01225 0.57 +1.4 301,447 + LRK53_RS01225 0.57 -1.4 301,447 + LRK53_RS01225 0.57 +0.4 301,447 + LRK53_RS01225 0.57 +0.4 301,447 + LRK53_RS01225 0.57 -1.1 301,447 + LRK53_RS01225 0.57 +2.4 301,447 + LRK53_RS01225 0.57 +1.0 301,448 - LRK53_RS01225 0.58 +2.0 301,448 - LRK53_RS01225 0.58 -0.4 301,448 - LRK53_RS01225 0.58 -0.2 301,448 - LRK53_RS01225 0.58 +1.4 301,448 - LRK53_RS01225 0.58 +0.4 301,448 - LRK53_RS01225 0.58 +1.0 301,448 - LRK53_RS01225 0.58 +0.6 301,579 + LRK53_RS01225 0.73 -0.6 301,579 + LRK53_RS01225 0.73 +0.4 301,579 + LRK53_RS01225 0.73 -0.9 301,579 + LRK53_RS01225 0.73 -0.6 301,579 + LRK53_RS01225 0.73 -1.7 301,579 + LRK53_RS01225 0.73 -0.6 301,579 + LRK53_RS01225 0.73 -1.4 301,579 + LRK53_RS01225 0.73 -1.9 301,579 + LRK53_RS01225 0.73 +1.4 301,579 + LRK53_RS01225 0.73 -0.1 301,579 + LRK53_RS01225 0.73 +0.5 301,579 + LRK53_RS01225 0.73 +0.2 301,579 + LRK53_RS01225 0.73 -0.9 301,579 + LRK53_RS01225 0.73 -1.2 301,579 + LRK53_RS01225 0.73 +1.7 301,579 + LRK53_RS01225 0.73 -0.4 301,579 + LRK53_RS01225 0.73 +0.4 301,579 + LRK53_RS01225 0.73 +0.4 301,579 + LRK53_RS01225 0.73 +0.7 301,579 + LRK53_RS01225 0.73 -0.6 301,579 + LRK53_RS01225 0.73 +0.8 301,580 - LRK53_RS01225 0.73 -0.4 301,580 - LRK53_RS01225 0.73 +1.4 301,580 - LRK53_RS01225 0.73 +0.8 301,580 - LRK53_RS01225 0.73 +1.4 301,580 - LRK53_RS01225 0.73 +0.4 301,580 - LRK53_RS01225 0.73 -0.2 301,580 - LRK53_RS01225 0.73 -1.9 301,580 - LRK53_RS01225 0.73 -0.1 301,580 - LRK53_RS01225 0.73 +0.8 301,580 - LRK53_RS01225 0.73 +0.8 301,580 - LRK53_RS01225 0.73 -0.5 301,580 - LRK53_RS01225 0.73 +0.1 301,616 + LRK53_RS01225 0.78 -0.9 301,616 + LRK53_RS01225 0.78 +0.6 301,616 + LRK53_RS01225 0.78 -0.6 301,616 + LRK53_RS01225 0.78 +0.4 301,616 + LRK53_RS01225 0.78 -0.8 301,617 - LRK53_RS01225 0.78 -0.2 301,712 + LRK53_RS01225 0.89 -1.2 301,712 + LRK53_RS01225 0.89 -0.5 301,712 + LRK53_RS01225 0.89 +0.6 301,712 + LRK53_RS01225 0.89 -0.1 301,712 + LRK53_RS01225 0.89 -0.4 301,712 + LRK53_RS01225 0.89 -3.5 301,712 + LRK53_RS01225 0.89 +0.4 301,713 - LRK53_RS01225 0.89 -1.2 301,713 - LRK53_RS01225 0.89 +1.4 301,713 - LRK53_RS01225 0.89 -0.2 301,713 - LRK53_RS01225 0.89 +1.4 301,713 - LRK53_RS01225 0.89 +0.4 301,713 - LRK53_RS01225 0.89 -1.1 301,738 - +0.4 301,759 + +0.2 301,759 + -0.9 301,759 + +2.4 301,759 + -0.2 301,760 - +0.8 301,760 - +0.4 301,760 - +0.3 301,760 - -0.0 301,760 - +0.2 301,760 - +1.4 301,851 + -0.2 301,852 - LRK53_RS01230 0.10 -0.0 301,852 - LRK53_RS01230 0.10 +0.4 301,966 + LRK53_RS01230 0.47 -1.4 301,966 + LRK53_RS01230 0.47 -0.7 301,967 - LRK53_RS01230 0.48 +0.2 301,967 - LRK53_RS01230 0.48 -1.1 301,967 - LRK53_RS01230 0.48 +1.4 302,083 - LRK53_RS01230 0.86 -0.8 302,169 - +1.4 302,170 + -0.4 302,170 + -0.2 302,170 + +0.5 302,170 + +0.8 302,170 + +0.8 302,170 + +0.0 302,170 + -0.2 302,170 + +1.4 302,170 + -0.9 302,170 + -0.7 302,170 + +1.4 302,170 + +3.0 302,170 + +0.1 302,170 + -0.9 302,170 + -0.1 302,170 + -0.8 302,171 - +0.8 302,171 - +1.0 302,171 - +0.8 302,171 - -0.4 302,171 - -1.3 302,171 - +1.0 302,171 - +0.4 302,171 - +0.1 302,171 - -0.0 302,171 - -1.2 302,192 + +0.4 302,192 + -0.2 302,192 + -0.6 302,192 + -0.1 302,192 + +0.8 302,193 - -0.6 302,193 - -1.4 302,193 - -0.2 302,193 - +1.4 302,193 - +0.4 302,193 - +0.4 302,241 + +0.2 302,241 + -0.2 302,241 + -0.5 302,242 - -1.6 302,242 - -0.4 302,283 + +0.4 302,283 + -0.7 302,284 - -0.2 302,284 - -0.4 302,311 - -0.4 302,733 + LRK53_RS01235 0.17 -0.4 302,733 + LRK53_RS01235 0.17 -1.4 302,733 + LRK53_RS01235 0.17 +1.4 302,734 - LRK53_RS01235 0.17 +0.4 302,734 - LRK53_RS01235 0.17 -0.9 302,995 + LRK53_RS01235 0.32 -1.2 304,069 - +1.4 304,104 + +3.0 304,105 - +0.6 304,248 + +2.4 304,312 + -0.2 304,312 + -0.9 304,313 - -1.4
Or see this region's nucleotide sequence