Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00900 and LRK53_RS00905 overlap by 4 nucleotides LRK53_RS00905 and LRK53_RS00910 are separated by 297 nucleotides LRK53_RS00910 and LRK53_RS00915 overlap by 1 nucleotides LRK53_RS00915 and LRK53_RS00920 overlap by 4 nucleotides
LRK53_RS00900: LRK53_RS00900 - ATP-binding protein, at 227,891 to 229,312
_RS00900
LRK53_RS00905: LRK53_RS00905 - response regulator, at 229,309 to 229,974
_RS00905
LRK53_RS00910: LRK53_RS00910 - monovalent cation/H+ antiporter subunit A, at 230,272 to 233,166
_RS00910
LRK53_RS00915: LRK53_RS00915 - Na+/H+ antiporter subunit C, at 233,166 to 233,507
_RS00915
LRK53_RS00920: LRK53_RS00920 - monovalent cation/H+ antiporter subunit D, at 233,504 to 235,099
_RS00920
Position (kb) 230
231
232
233
234 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 229.334 kb on + strand at 229.334 kb on + strand at 229.334 kb on + strand at 229.335 kb on - strand at 229.335 kb on - strand at 229.439 kb on + strand, within LRK53_RS00905 at 229.440 kb on - strand, within LRK53_RS00905 at 229.440 kb on - strand, within LRK53_RS00905 at 229.682 kb on + strand, within LRK53_RS00905 at 229.682 kb on + strand, within LRK53_RS00905 at 229.682 kb on + strand, within LRK53_RS00905 at 229.682 kb on + strand, within LRK53_RS00905 at 229.682 kb on + strand, within LRK53_RS00905 at 229.683 kb on - strand, within LRK53_RS00905 at 229.683 kb on - strand, within LRK53_RS00905 at 229.770 kb on + strand, within LRK53_RS00905 at 229.899 kb on + strand, within LRK53_RS00905 at 229.899 kb on + strand, within LRK53_RS00905 at 229.899 kb on + strand, within LRK53_RS00905 at 229.899 kb on + strand, within LRK53_RS00905 at 229.899 kb on + strand, within LRK53_RS00905 at 229.899 kb on + strand, within LRK53_RS00905 at 229.900 kb on - strand, within LRK53_RS00905 at 229.901 kb on + strand, within LRK53_RS00905 at 229.902 kb on - strand, within LRK53_RS00905 at 229.902 kb on - strand, within LRK53_RS00905 at 230.090 kb on - strand at 230.090 kb on - strand at 230.091 kb on + strand at 230.091 kb on + strand at 230.091 kb on + strand at 230.091 kb on + strand at 230.091 kb on + strand at 230.092 kb on - strand at 230.402 kb on + strand at 230.560 kb on + strand at 230.560 kb on + strand at 230.560 kb on + strand at 230.560 kb on + strand at 230.560 kb on + strand at 230.560 kb on + strand at 230.560 kb on + strand at 230.560 kb on + strand at 230.561 kb on - strand at 230.561 kb on - strand at 230.561 kb on - strand at 230.561 kb on - strand at 230.570 kb on - strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.572 kb on + strand, within LRK53_RS00910 at 230.573 kb on - strand, within LRK53_RS00910 at 230.573 kb on - strand, within LRK53_RS00910 at 230.573 kb on - strand, within LRK53_RS00910 at 230.573 kb on - strand, within LRK53_RS00910 at 230.573 kb on - strand, within LRK53_RS00910 at 230.573 kb on - strand, within LRK53_RS00910 at 230.573 kb on - strand, within LRK53_RS00910 at 230.605 kb on + strand, within LRK53_RS00910 at 230.605 kb on + strand, within LRK53_RS00910 at 230.605 kb on + strand, within LRK53_RS00910 at 230.605 kb on + strand, within LRK53_RS00910 at 230.606 kb on - strand, within LRK53_RS00910 at 230.606 kb on - strand, within LRK53_RS00910 at 230.723 kb on - strand, within LRK53_RS00910 at 230.826 kb on + strand, within LRK53_RS00910 at 230.827 kb on - strand, within LRK53_RS00910 at 230.840 kb on - strand, within LRK53_RS00910 at 230.840 kb on - strand, within LRK53_RS00910 at 230.893 kb on + strand, within LRK53_RS00910 at 230.893 kb on + strand, within LRK53_RS00910 at 230.893 kb on + strand, within LRK53_RS00910 at 230.894 kb on - strand, within LRK53_RS00910 at 230.894 kb on - strand, within LRK53_RS00910 at 230.894 kb on - strand, within LRK53_RS00910 at 231.004 kb on + strand, within LRK53_RS00910 at 231.004 kb on + strand, within LRK53_RS00910 at 231.004 kb on + strand, within LRK53_RS00910 at 231.179 kb on - strand, within LRK53_RS00910 at 231.550 kb on + strand, within LRK53_RS00910 at 231.550 kb on + strand, within LRK53_RS00910 at 231.550 kb on + strand, within LRK53_RS00910 at 231.550 kb on + strand, within LRK53_RS00910 at 231.551 kb on - strand, within LRK53_RS00910 at 231.760 kb on + strand, within LRK53_RS00910 at 231.760 kb on + strand, within LRK53_RS00910 at 231.760 kb on + strand, within LRK53_RS00910 at 232.417 kb on + strand, within LRK53_RS00910 at 232.417 kb on + strand, within LRK53_RS00910 at 232.417 kb on + strand, within LRK53_RS00910 at 232.417 kb on + strand, within LRK53_RS00910 at 232.417 kb on + strand, within LRK53_RS00910 at 232.751 kb on + strand, within LRK53_RS00910 at 232.752 kb on - strand, within LRK53_RS00910 at 232.752 kb on - strand, within LRK53_RS00910 at 232.852 kb on + strand, within LRK53_RS00910 at 232.852 kb on + strand, within LRK53_RS00910 at 232.852 kb on + strand, within LRK53_RS00910 at 232.852 kb on + strand, within LRK53_RS00910 at 232.852 kb on + strand, within LRK53_RS00910 at 232.852 kb on + strand, within LRK53_RS00910 at 232.852 kb on + strand, within LRK53_RS00910 at 232.852 kb on + strand, within LRK53_RS00910 at 232.853 kb on - strand, within LRK53_RS00910 at 232.853 kb on - strand, within LRK53_RS00910 at 232.853 kb on - strand, within LRK53_RS00910 at 232.853 kb on - strand, within LRK53_RS00910 at 232.853 kb on - strand, within LRK53_RS00910 at 232.853 kb on - strand, within LRK53_RS00910 at 233.197 kb on + strand at 233.198 kb on - strand at 233.274 kb on + strand, within LRK53_RS00915 at 233.275 kb on - strand, within LRK53_RS00915 at 233.275 kb on - strand, within LRK53_RS00915 at 233.364 kb on + strand, within LRK53_RS00915 at 233.687 kb on + strand, within LRK53_RS00920 at 233.937 kb on - strand, within LRK53_RS00920 at 233.990 kb on + strand, within LRK53_RS00920 at 233.990 kb on + strand, within LRK53_RS00920 at 233.990 kb on + strand, within LRK53_RS00920 at 233.990 kb on + strand, within LRK53_RS00920 at 233.990 kb on + strand, within LRK53_RS00920 at 233.990 kb on + strand, within LRK53_RS00920 at 233.990 kb on + strand, within LRK53_RS00920 at 233.990 kb on + strand, within LRK53_RS00920 at 233.990 kb on + strand, within LRK53_RS00920 at 233.991 kb on - strand, within LRK53_RS00920 at 233.991 kb on - strand, within LRK53_RS00920 at 233.991 kb on - strand, within LRK53_RS00920
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 229,334 + +0.7 229,334 + +0.7 229,334 + -1.8 229,335 - +0.2 229,335 - +0.1 229,439 + LRK53_RS00905 0.20 -0.9 229,440 - LRK53_RS00905 0.20 +0.4 229,440 - LRK53_RS00905 0.20 +3.0 229,682 + LRK53_RS00905 0.56 +0.4 229,682 + LRK53_RS00905 0.56 -2.3 229,682 + LRK53_RS00905 0.56 -0.4 229,682 + LRK53_RS00905 0.56 -0.2 229,682 + LRK53_RS00905 0.56 +0.7 229,683 - LRK53_RS00905 0.56 +0.0 229,683 - LRK53_RS00905 0.56 -0.2 229,770 + LRK53_RS00905 0.69 -0.2 229,899 + LRK53_RS00905 0.89 +1.4 229,899 + LRK53_RS00905 0.89 +0.4 229,899 + LRK53_RS00905 0.89 -0.2 229,899 + LRK53_RS00905 0.89 -1.8 229,899 + LRK53_RS00905 0.89 -0.2 229,899 + LRK53_RS00905 0.89 +0.4 229,900 - LRK53_RS00905 0.89 +0.4 229,901 + LRK53_RS00905 0.89 -0.2 229,902 - LRK53_RS00905 0.89 -0.6 229,902 - LRK53_RS00905 0.89 -0.2 230,090 - +1.4 230,090 - +1.4 230,091 + -0.2 230,091 + -1.2 230,091 + -1.0 230,091 + +2.4 230,091 + -1.1 230,092 - -0.4 230,402 + +1.4 230,560 + -0.2 230,560 + -1.2 230,560 + +0.8 230,560 + -0.6 230,560 + -0.8 230,560 + +1.4 230,560 + +0.8 230,560 + -0.6 230,561 - +0.4 230,561 - +0.8 230,561 - +0.4 230,561 - -1.3 230,570 - LRK53_RS00910 0.10 +1.4 230,572 + LRK53_RS00910 0.10 +0.4 230,572 + LRK53_RS00910 0.10 +0.4 230,572 + LRK53_RS00910 0.10 -0.6 230,572 + LRK53_RS00910 0.10 +0.4 230,572 + LRK53_RS00910 0.10 +2.0 230,572 + LRK53_RS00910 0.10 +0.4 230,572 + LRK53_RS00910 0.10 -0.2 230,572 + LRK53_RS00910 0.10 -0.4 230,572 + LRK53_RS00910 0.10 -0.2 230,572 + LRK53_RS00910 0.10 +0.4 230,572 + LRK53_RS00910 0.10 +1.4 230,572 + LRK53_RS00910 0.10 +2.0 230,572 + LRK53_RS00910 0.10 +1.4 230,572 + LRK53_RS00910 0.10 -0.6 230,573 - LRK53_RS00910 0.10 -0.4 230,573 - LRK53_RS00910 0.10 +2.0 230,573 - LRK53_RS00910 0.10 -1.2 230,573 - LRK53_RS00910 0.10 -3.2 230,573 - LRK53_RS00910 0.10 -0.9 230,573 - LRK53_RS00910 0.10 +0.4 230,573 - LRK53_RS00910 0.10 -0.7 230,605 + LRK53_RS00910 0.12 +1.4 230,605 + LRK53_RS00910 0.12 -1.8 230,605 + LRK53_RS00910 0.12 +0.4 230,605 + LRK53_RS00910 0.12 +0.8 230,606 - LRK53_RS00910 0.12 +0.8 230,606 - LRK53_RS00910 0.12 +1.4 230,723 - LRK53_RS00910 0.16 +0.4 230,826 + LRK53_RS00910 0.19 -1.6 230,827 - LRK53_RS00910 0.19 +0.8 230,840 - LRK53_RS00910 0.20 -0.9 230,840 - LRK53_RS00910 0.20 +1.4 230,893 + LRK53_RS00910 0.21 +0.8 230,893 + LRK53_RS00910 0.21 +0.4 230,893 + LRK53_RS00910 0.21 -0.9 230,894 - LRK53_RS00910 0.21 -0.9 230,894 - LRK53_RS00910 0.21 -0.2 230,894 - LRK53_RS00910 0.21 +1.4 231,004 + LRK53_RS00910 0.25 -0.2 231,004 + LRK53_RS00910 0.25 -2.1 231,004 + LRK53_RS00910 0.25 -1.2 231,179 - LRK53_RS00910 0.31 -0.2 231,550 + LRK53_RS00910 0.44 -1.6 231,550 + LRK53_RS00910 0.44 -1.4 231,550 + LRK53_RS00910 0.44 -0.2 231,550 + LRK53_RS00910 0.44 -0.2 231,551 - LRK53_RS00910 0.44 -0.2 231,760 + LRK53_RS00910 0.51 +1.4 231,760 + LRK53_RS00910 0.51 -1.2 231,760 + LRK53_RS00910 0.51 +2.0 232,417 + LRK53_RS00910 0.74 -1.7 232,417 + LRK53_RS00910 0.74 +0.4 232,417 + LRK53_RS00910 0.74 +0.4 232,417 + LRK53_RS00910 0.74 -0.6 232,417 + LRK53_RS00910 0.74 +1.4 232,751 + LRK53_RS00910 0.86 -0.6 232,752 - LRK53_RS00910 0.86 +0.4 232,752 - LRK53_RS00910 0.86 -1.2 232,852 + LRK53_RS00910 0.89 -0.6 232,852 + LRK53_RS00910 0.89 +0.2 232,852 + LRK53_RS00910 0.89 -0.4 232,852 + LRK53_RS00910 0.89 -1.0 232,852 + LRK53_RS00910 0.89 -0.6 232,852 + LRK53_RS00910 0.89 -0.1 232,852 + LRK53_RS00910 0.89 -1.2 232,852 + LRK53_RS00910 0.89 -1.4 232,853 - LRK53_RS00910 0.89 -1.8 232,853 - LRK53_RS00910 0.89 +2.4 232,853 - LRK53_RS00910 0.89 -0.6 232,853 - LRK53_RS00910 0.89 +0.1 232,853 - LRK53_RS00910 0.89 -1.8 232,853 - LRK53_RS00910 0.89 +0.4 233,197 + +0.4 233,198 - +2.4 233,274 + LRK53_RS00915 0.32 -0.2 233,275 - LRK53_RS00915 0.32 +0.1 233,275 - LRK53_RS00915 0.32 -0.2 233,364 + LRK53_RS00915 0.58 -2.1 233,687 + LRK53_RS00920 0.11 +1.4 233,937 - LRK53_RS00920 0.27 +0.7 233,990 + LRK53_RS00920 0.30 +1.4 233,990 + LRK53_RS00920 0.30 -1.1 233,990 + LRK53_RS00920 0.30 +0.4 233,990 + LRK53_RS00920 0.30 -1.8 233,990 + LRK53_RS00920 0.30 -1.2 233,990 + LRK53_RS00920 0.30 -0.2 233,990 + LRK53_RS00920 0.30 +0.4 233,990 + LRK53_RS00920 0.30 -1.2 233,990 + LRK53_RS00920 0.30 +1.4 233,991 - LRK53_RS00920 0.31 -0.0 233,991 - LRK53_RS00920 0.31 +1.4 233,991 - LRK53_RS00920 0.31 -1.6
Or see this region's nucleotide sequence