Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18210 and LRK53_RS18215 are separated by 252 nucleotides LRK53_RS18215 and LRK53_RS18220 are separated by 73 nucleotides LRK53_RS18220 and LRK53_RS18225 are separated by 6 nucleotides
LRK53_RS18210: LRK53_RS18210 - PRTRC system ThiF family protein, at 18,195 to 19,004
_RS18210
LRK53_RS18215: LRK53_RS18215 - single-stranded DNA-binding protein, at 19,257 to 19,847
_RS18215
LRK53_RS18220: LRK53_RS18220 - hypothetical protein, at 19,921 to 20,070
_RS18220
LRK53_RS18225: LRK53_RS18225 - DEAD/DEAH box helicase, at 20,077 to 22,062
_RS18225
Position (kb) 19
20 Strain fitness (log2 ratio) -2
-1
0
1
2 at 18.405 kb on + strand, within LRK53_RS18210 at 18.405 kb on + strand, within LRK53_RS18210 at 18.406 kb on - strand, within LRK53_RS18210 at 18.406 kb on - strand, within LRK53_RS18210 at 18.496 kb on - strand, within LRK53_RS18210 at 18.551 kb on + strand, within LRK53_RS18210 at 18.717 kb on + strand, within LRK53_RS18210 at 18.882 kb on + strand, within LRK53_RS18210 at 18.882 kb on + strand, within LRK53_RS18210 at 18.883 kb on - strand, within LRK53_RS18210 at 18.883 kb on - strand, within LRK53_RS18210 at 18.883 kb on - strand, within LRK53_RS18210 at 18.883 kb on - strand, within LRK53_RS18210 at 18.883 kb on - strand, within LRK53_RS18210 at 18.903 kb on + strand, within LRK53_RS18210 at 18.903 kb on + strand, within LRK53_RS18210 at 18.903 kb on + strand, within LRK53_RS18210 at 18.903 kb on + strand, within LRK53_RS18210 at 18.903 kb on + strand, within LRK53_RS18210 at 18.903 kb on + strand, within LRK53_RS18210 at 18.904 kb on - strand, within LRK53_RS18210 at 18.904 kb on - strand, within LRK53_RS18210 at 18.904 kb on - strand, within LRK53_RS18210 at 18.904 kb on - strand, within LRK53_RS18210 at 18.904 kb on - strand, within LRK53_RS18210 at 18.904 kb on - strand, within LRK53_RS18210 at 18.904 kb on - strand, within LRK53_RS18210 at 18.904 kb on - strand, within LRK53_RS18210 at 18.904 kb on - strand, within LRK53_RS18210 at 18.957 kb on + strand at 18.957 kb on + strand at 18.957 kb on + strand at 18.958 kb on - strand at 19.049 kb on - strand at 19.049 kb on - strand at 19.074 kb on + strand at 19.231 kb on + strand at 19.231 kb on + strand at 19.231 kb on + strand at 19.232 kb on - strand at 19.232 kb on - strand at 19.232 kb on - strand at 19.238 kb on + strand at 19.239 kb on - strand at 19.239 kb on - strand at 19.256 kb on + strand at 19.256 kb on + strand at 19.256 kb on + strand at 19.257 kb on - strand at 19.286 kb on + strand at 19.286 kb on + strand at 19.286 kb on + strand at 19.287 kb on - strand at 19.287 kb on - strand at 19.467 kb on + strand, within LRK53_RS18215 at 19.467 kb on + strand, within LRK53_RS18215 at 19.467 kb on + strand, within LRK53_RS18215 at 19.467 kb on + strand, within LRK53_RS18215 at 19.468 kb on - strand, within LRK53_RS18215 at 19.468 kb on - strand, within LRK53_RS18215 at 19.468 kb on - strand, within LRK53_RS18215 at 19.468 kb on - strand, within LRK53_RS18215 at 19.491 kb on + strand, within LRK53_RS18215 at 19.492 kb on - strand, within LRK53_RS18215 at 19.492 kb on - strand, within LRK53_RS18215 at 19.521 kb on + strand, within LRK53_RS18215 at 19.521 kb on + strand, within LRK53_RS18215 at 19.521 kb on + strand, within LRK53_RS18215 at 19.521 kb on + strand, within LRK53_RS18215 at 19.521 kb on + strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.723 kb on - strand, within LRK53_RS18215 at 19.835 kb on + strand at 19.836 kb on - strand at 19.836 kb on - strand at 19.838 kb on - strand at 19.845 kb on + strand at 19.869 kb on - strand at 19.869 kb on - strand at 19.870 kb on + strand at 19.893 kb on - strand at 20.068 kb on + strand at 20.069 kb on - strand at 20.179 kb on + strand at 20.179 kb on + strand at 20.180 kb on - strand at 20.180 kb on - strand at 20.494 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.546 kb on - strand, within LRK53_RS18225 at 20.546 kb on - strand, within LRK53_RS18225 at 20.546 kb on - strand, within LRK53_RS18225 at 20.546 kb on - strand, within LRK53_RS18225 at 20.546 kb on - strand, within LRK53_RS18225 at 20.579 kb on - strand, within LRK53_RS18225 at 20.698 kb on + strand, within LRK53_RS18225 at 20.699 kb on - strand, within LRK53_RS18225 at 20.699 kb on - strand, within LRK53_RS18225 at 20.699 kb on - strand, within LRK53_RS18225 at 20.722 kb on + strand, within LRK53_RS18225
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 18,405 + LRK53_RS18210 0.26 -1.7 18,405 + LRK53_RS18210 0.26 -1.0 18,406 - LRK53_RS18210 0.26 +0.3 18,406 - LRK53_RS18210 0.26 -0.2 18,496 - LRK53_RS18210 0.37 -0.6 18,551 + LRK53_RS18210 0.44 +1.0 18,717 + LRK53_RS18210 0.64 +0.1 18,882 + LRK53_RS18210 0.85 -0.6 18,882 + LRK53_RS18210 0.85 +1.6 18,883 - LRK53_RS18210 0.85 +0.4 18,883 - LRK53_RS18210 0.85 -1.0 18,883 - LRK53_RS18210 0.85 -1.0 18,883 - LRK53_RS18210 0.85 -0.3 18,883 - LRK53_RS18210 0.85 -1.0 18,903 + LRK53_RS18210 0.87 -0.2 18,903 + LRK53_RS18210 0.87 +0.3 18,903 + LRK53_RS18210 0.87 -1.7 18,903 + LRK53_RS18210 0.87 +1.8 18,903 + LRK53_RS18210 0.87 -1.1 18,903 + LRK53_RS18210 0.87 -0.7 18,904 - LRK53_RS18210 0.88 -1.0 18,904 - LRK53_RS18210 0.88 -1.0 18,904 - LRK53_RS18210 0.88 +1.0 18,904 - LRK53_RS18210 0.88 +0.1 18,904 - LRK53_RS18210 0.88 +0.1 18,904 - LRK53_RS18210 0.88 +0.2 18,904 - LRK53_RS18210 0.88 +0.0 18,904 - LRK53_RS18210 0.88 -1.8 18,904 - LRK53_RS18210 0.88 +2.0 18,957 + -0.8 18,957 + +1.0 18,957 + -1.3 18,958 - -0.7 19,049 - -0.6 19,049 - +0.7 19,074 + +2.0 19,231 + +1.4 19,231 + -1.5 19,231 + -0.6 19,232 - -0.6 19,232 - -0.6 19,232 - +0.0 19,238 + +0.6 19,239 - -1.2 19,239 - -0.4 19,256 + -0.3 19,256 + -0.7 19,256 + +1.0 19,257 - -1.2 19,286 + -0.4 19,286 + -0.8 19,286 + -0.2 19,287 - +0.5 19,287 - -1.1 19,467 + LRK53_RS18215 0.36 -0.1 19,467 + LRK53_RS18215 0.36 +1.0 19,467 + LRK53_RS18215 0.36 +0.4 19,467 + LRK53_RS18215 0.36 -0.4 19,468 - LRK53_RS18215 0.36 -0.6 19,468 - LRK53_RS18215 0.36 -0.4 19,468 - LRK53_RS18215 0.36 -1.0 19,468 - LRK53_RS18215 0.36 +0.6 19,491 + LRK53_RS18215 0.40 +0.1 19,492 - LRK53_RS18215 0.40 +0.6 19,492 - LRK53_RS18215 0.40 -1.2 19,521 + LRK53_RS18215 0.45 +0.3 19,521 + LRK53_RS18215 0.45 +0.0 19,521 + LRK53_RS18215 0.45 +0.8 19,521 + LRK53_RS18215 0.45 -1.2 19,521 + LRK53_RS18215 0.45 -0.3 19,522 - LRK53_RS18215 0.45 -1.0 19,522 - LRK53_RS18215 0.45 +0.4 19,522 - LRK53_RS18215 0.45 -0.4 19,522 - LRK53_RS18215 0.45 +1.0 19,522 - LRK53_RS18215 0.45 -0.1 19,522 - LRK53_RS18215 0.45 -0.6 19,522 - LRK53_RS18215 0.45 -0.6 19,522 - LRK53_RS18215 0.45 +0.5 19,522 - LRK53_RS18215 0.45 -0.5 19,723 - LRK53_RS18215 0.79 -0.8 19,835 + +0.7 19,836 - +0.0 19,836 - -1.0 19,838 - -1.6 19,845 + -0.5 19,869 - -1.0 19,869 - +0.6 19,870 + -1.4 19,893 - +0.3 20,068 + -1.6 20,069 - -0.4 20,179 + -1.0 20,179 + -0.4 20,180 - -0.6 20,180 - +1.0 20,494 + LRK53_RS18225 0.21 +1.0 20,545 + LRK53_RS18225 0.24 -1.0 20,545 + LRK53_RS18225 0.24 -0.7 20,545 + LRK53_RS18225 0.24 -0.3 20,545 + LRK53_RS18225 0.24 +1.0 20,545 + LRK53_RS18225 0.24 -0.6 20,545 + LRK53_RS18225 0.24 +0.2 20,545 + LRK53_RS18225 0.24 -1.2 20,545 + LRK53_RS18225 0.24 -1.3 20,545 + LRK53_RS18225 0.24 -0.6 20,546 - LRK53_RS18225 0.24 -0.6 20,546 - LRK53_RS18225 0.24 +0.3 20,546 - LRK53_RS18225 0.24 -1.3 20,546 - LRK53_RS18225 0.24 -0.7 20,546 - LRK53_RS18225 0.24 -2.0 20,579 - LRK53_RS18225 0.25 +0.2 20,698 + LRK53_RS18225 0.31 +0.4 20,699 - LRK53_RS18225 0.31 -0.9 20,699 - LRK53_RS18225 0.31 +1.0 20,699 - LRK53_RS18225 0.31 +1.0 20,722 + LRK53_RS18225 0.32 +0.3
Or see this region's nucleotide sequence