Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS11385 and LRK53_RS11390 are separated by 154 nucleotides LRK53_RS11390 and LRK53_RS11395 are separated by 83 nucleotides LRK53_RS11395 and LRK53_RS11400 are separated by 123 nucleotides
LRK53_RS11385: LRK53_RS11385 - molecular chaperone DnaK, at 2,537,998 to 2,539,914
_RS11385
LRK53_RS11390: LRK53_RS11390 - nucleotide exchange factor GrpE, at 2,540,069 to 2,540,602
_RS11390
LRK53_RS11395: LRK53_RS11395 - heat-inducible transcriptional repressor HrcA, at 2,540,686 to 2,541,756
_RS11395
LRK53_RS11400: LRK53_RS11400 - DNA repair protein RecN, at 2,541,880 to 2,543,553
_RS11400
Position (kb)
2540
2541
2542 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2540.666 kb on + strand at 2540.667 kb on - strand at 2540.687 kb on + strand at 2540.688 kb on - strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.689 kb on + strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.690 kb on - strand at 2540.765 kb on + strand at 2540.765 kb on + strand at 2540.765 kb on + strand at 2540.765 kb on + strand at 2540.765 kb on + strand at 2540.765 kb on + strand at 2540.766 kb on - strand at 2540.825 kb on + strand, within LRK53_RS11395 at 2540.825 kb on + strand, within LRK53_RS11395 at 2540.826 kb on - strand, within LRK53_RS11395 at 2540.999 kb on + strand, within LRK53_RS11395 at 2540.999 kb on + strand, within LRK53_RS11395 at 2541.000 kb on - strand, within LRK53_RS11395 at 2541.000 kb on - strand, within LRK53_RS11395 at 2541.197 kb on + strand, within LRK53_RS11395 at 2541.197 kb on + strand, within LRK53_RS11395 at 2541.197 kb on + strand, within LRK53_RS11395 at 2541.198 kb on - strand, within LRK53_RS11395 at 2541.198 kb on - strand, within LRK53_RS11395 at 2541.558 kb on + strand, within LRK53_RS11395 at 2541.558 kb on + strand, within LRK53_RS11395 at 2541.558 kb on + strand, within LRK53_RS11395 at 2541.558 kb on + strand, within LRK53_RS11395 at 2541.558 kb on + strand, within LRK53_RS11395 at 2541.558 kb on + strand, within LRK53_RS11395 at 2541.558 kb on + strand, within LRK53_RS11395 at 2541.559 kb on - strand, within LRK53_RS11395 at 2541.559 kb on - strand, within LRK53_RS11395 at 2541.559 kb on - strand, within LRK53_RS11395 at 2541.559 kb on - strand, within LRK53_RS11395 at 2541.559 kb on - strand, within LRK53_RS11395 at 2541.559 kb on - strand, within LRK53_RS11395 at 2541.559 kb on - strand, within LRK53_RS11395 at 2541.686 kb on + strand at 2541.686 kb on + strand at 2541.686 kb on + strand at 2541.686 kb on + strand at 2541.686 kb on + strand at 2541.686 kb on + strand at 2541.687 kb on - strand at 2541.687 kb on - strand at 2541.687 kb on - strand at 2541.687 kb on - strand at 2541.764 kb on + strand at 2541.764 kb on + strand at 2541.764 kb on + strand at 2541.765 kb on - strand at 2541.765 kb on - strand at 2541.765 kb on - strand at 2541.765 kb on - strand at 2541.765 kb on - strand at 2541.790 kb on + strand at 2541.790 kb on + strand at 2541.791 kb on - strand at 2541.791 kb on - strand at 2541.791 kb on - strand at 2541.848 kb on + strand at 2541.895 kb on + strand at 2541.895 kb on + strand at 2541.895 kb on + strand at 2541.895 kb on + strand at 2541.895 kb on + strand at 2541.895 kb on + strand at 2541.895 kb on + strand at 2541.896 kb on - strand at 2541.896 kb on - strand at 2541.896 kb on - strand at 2541.896 kb on - strand at 2541.896 kb on - strand at 2541.896 kb on - strand at 2541.896 kb on - strand at 2541.896 kb on - strand at 2541.896 kb on - strand at 2541.896 kb on - strand at 2542.043 kb on + strand at 2542.043 kb on + strand at 2542.043 kb on + strand at 2542.043 kb on + strand at 2542.043 kb on + strand at 2542.043 kb on + strand at 2542.043 kb on + strand at 2542.043 kb on + strand at 2542.043 kb on + strand at 2542.043 kb on + strand at 2542.044 kb on - strand at 2542.044 kb on - strand at 2542.324 kb on + strand, within LRK53_RS11400 at 2542.325 kb on - strand, within LRK53_RS11400 at 2542.698 kb on + strand, within LRK53_RS11400 at 2542.735 kb on + strand, within LRK53_RS11400 at 2542.736 kb on - strand, within LRK53_RS11400 at 2542.736 kb on - strand, within LRK53_RS11400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 2,540,666 + +0.9 2,540,667 - -0.1 2,540,687 + +0.5 2,540,688 - -1.3 2,540,689 + +1.3 2,540,689 + +0.3 2,540,689 + -0.4 2,540,689 + +0.5 2,540,689 + +0.3 2,540,689 + +0.7 2,540,689 + -0.4 2,540,689 + -1.4 2,540,689 + +1.9 2,540,689 + +1.9 2,540,689 + +0.3 2,540,689 + +1.9 2,540,689 + +0.6 2,540,689 + +0.3 2,540,689 + +0.3 2,540,689 + +0.8 2,540,689 + +0.3 2,540,689 + +0.7 2,540,689 + +1.9 2,540,689 + +0.5 2,540,689 + +1.1 2,540,689 + +0.9 2,540,689 + -0.1 2,540,689 + +0.6 2,540,689 + +3.2 2,540,689 + -0.4 2,540,689 + +1.2 2,540,689 + +1.9 2,540,689 + +0.9 2,540,689 + +1.1 2,540,689 + +0.6 2,540,689 + +0.9 2,540,689 + +0.4 2,540,689 + +0.7 2,540,689 + +0.3 2,540,690 - +0.9 2,540,690 - +1.5 2,540,690 - +0.3 2,540,690 - -1.4 2,540,690 - +0.7 2,540,690 - -1.1 2,540,690 - -0.7 2,540,690 - -0.1 2,540,690 - -1.3 2,540,690 - +0.9 2,540,690 - +0.9 2,540,690 - +0.9 2,540,690 - -0.5 2,540,690 - -0.7 2,540,690 - +2.9 2,540,690 - -0.1 2,540,690 - -0.1 2,540,690 - -1.7 2,540,690 - +0.9 2,540,690 - -0.3 2,540,765 + +0.4 2,540,765 + +0.0 2,540,765 + -0.7 2,540,765 + +0.9 2,540,765 + +2.5 2,540,765 + +0.2 2,540,766 - -0.6 2,540,825 + LRK53_RS11395 0.13 +2.2 2,540,825 + LRK53_RS11395 0.13 +0.4 2,540,826 - LRK53_RS11395 0.13 +0.9 2,540,999 + LRK53_RS11395 0.29 +0.1 2,540,999 + LRK53_RS11395 0.29 +0.3 2,541,000 - LRK53_RS11395 0.29 -1.2 2,541,000 - LRK53_RS11395 0.29 +0.6 2,541,197 + LRK53_RS11395 0.48 -0.1 2,541,197 + LRK53_RS11395 0.48 -0.4 2,541,197 + LRK53_RS11395 0.48 -0.6 2,541,198 - LRK53_RS11395 0.48 -1.1 2,541,198 - LRK53_RS11395 0.48 -0.2 2,541,558 + LRK53_RS11395 0.81 +0.1 2,541,558 + LRK53_RS11395 0.81 +0.9 2,541,558 + LRK53_RS11395 0.81 +1.9 2,541,558 + LRK53_RS11395 0.81 -0.0 2,541,558 + LRK53_RS11395 0.81 +0.3 2,541,558 + LRK53_RS11395 0.81 +0.3 2,541,558 + LRK53_RS11395 0.81 +0.9 2,541,559 - LRK53_RS11395 0.82 -0.6 2,541,559 - LRK53_RS11395 0.82 +1.9 2,541,559 - LRK53_RS11395 0.82 -1.0 2,541,559 - LRK53_RS11395 0.82 -0.7 2,541,559 - LRK53_RS11395 0.82 -0.7 2,541,559 - LRK53_RS11395 0.82 -1.4 2,541,559 - LRK53_RS11395 0.82 +0.6 2,541,686 + -0.1 2,541,686 + -0.3 2,541,686 + +0.3 2,541,686 + -1.9 2,541,686 + +0.3 2,541,686 + -0.5 2,541,687 - -0.1 2,541,687 - -1.5 2,541,687 - +1.1 2,541,687 - -0.7 2,541,764 + +2.5 2,541,764 + +0.5 2,541,764 + -0.1 2,541,765 - -0.1 2,541,765 - -1.1 2,541,765 - +1.9 2,541,765 - -0.1 2,541,765 - -1.1 2,541,790 + -0.7 2,541,790 + +0.3 2,541,791 - -1.4 2,541,791 - -0.4 2,541,791 - -0.1 2,541,848 + +0.9 2,541,895 + -0.1 2,541,895 + +1.5 2,541,895 + -0.7 2,541,895 + -0.7 2,541,895 + +0.9 2,541,895 + +0.5 2,541,895 + -0.5 2,541,896 - +0.3 2,541,896 - +1.5 2,541,896 - +1.9 2,541,896 - -0.7 2,541,896 - -0.1 2,541,896 - +0.9 2,541,896 - +0.9 2,541,896 - +1.9 2,541,896 - -0.9 2,541,896 - -1.1 2,542,043 + -1.1 2,542,043 + +0.5 2,542,043 + -1.1 2,542,043 + +0.9 2,542,043 + +1.3 2,542,043 + +0.4 2,542,043 + -1.7 2,542,043 + +0.9 2,542,043 + +0.9 2,542,043 + -0.7 2,542,044 - +0.9 2,542,044 - -0.2 2,542,324 + LRK53_RS11400 0.27 -0.4 2,542,325 - LRK53_RS11400 0.27 -1.0 2,542,698 + LRK53_RS11400 0.49 -2.4 2,542,735 + LRK53_RS11400 0.51 -1.4 2,542,736 - LRK53_RS11400 0.51 +0.2 2,542,736 - LRK53_RS11400 0.51 +0.9
Or see this region's nucleotide sequence