Strain Fitness in Rhodanobacter sp000427505 FW510-R12 around LRK53_RS11255

Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntLRK53_RS11245 and LRK53_RS11250 overlap by 4 nucleotidesLRK53_RS11250 and LRK53_RS11255 overlap by 4 nucleotidesLRK53_RS11255 and LRK53_RS11260 overlap by 11 nucleotidesLRK53_RS11260 and LRK53_RS11265 overlap by 20 nucleotidesLRK53_RS11265 and LRK53_RS11270 are separated by 65 nucleotides LRK53_RS11245: LRK53_RS11245 - bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada, at 2,510,944 to 2,512,020 _RS11245 LRK53_RS11250: LRK53_RS11250 - 2OG-Fe(II) oxygenase, at 2,512,017 to 2,512,748 _RS11250 LRK53_RS11255: LRK53_RS11255 - DNA oxidative demethylase AlkB, at 2,512,745 to 2,513,401 _RS11255 LRK53_RS11260: LRK53_RS11260 - DNA-3-methyladenine glycosylase, at 2,513,391 to 2,514,023 _RS11260 LRK53_RS11265: LRK53_RS11265 - hypothetical protein, at 2,514,004 to 2,514,189 _RS11265 LRK53_RS11270: LRK53_RS11270 - MBL fold metallo-hydrolase, at 2,514,255 to 2,515,196 _RS11270 Position (kb) 2512 2513 2514Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2511.788 kb on + strand, within LRK53_RS11245at 2511.788 kb on + strand, within LRK53_RS11245at 2511.789 kb on - strand, within LRK53_RS11245at 2511.789 kb on - strand, within LRK53_RS11245at 2511.789 kb on - strand, within LRK53_RS11245at 2511.832 kb on + strand, within LRK53_RS11245at 2511.833 kb on - strand, within LRK53_RS11245at 2511.833 kb on - strand, within LRK53_RS11245at 2511.833 kb on - strand, within LRK53_RS11245at 2512.068 kb on + strandat 2512.068 kb on + strandat 2512.068 kb on + strandat 2512.068 kb on + strandat 2512.068 kb on + strandat 2512.069 kb on - strandat 2512.069 kb on - strandat 2512.069 kb on - strandat 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.167 kb on + strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.168 kb on - strand, within LRK53_RS11250at 2512.233 kb on + strand, within LRK53_RS11250at 2512.234 kb on - strand, within LRK53_RS11250at 2512.234 kb on - strand, within LRK53_RS11250at 2512.239 kb on + strand, within LRK53_RS11250at 2512.239 kb on + strand, within LRK53_RS11250at 2512.290 kb on + strand, within LRK53_RS11250at 2512.290 kb on + strand, within LRK53_RS11250at 2512.290 kb on + strand, within LRK53_RS11250at 2512.290 kb on + strand, within LRK53_RS11250at 2512.290 kb on + strand, within LRK53_RS11250at 2512.428 kb on + strand, within LRK53_RS11250at 2512.428 kb on + strand, within LRK53_RS11250at 2512.429 kb on - strand, within LRK53_RS11250at 2512.429 kb on - strand, within LRK53_RS11250at 2512.443 kb on + strand, within LRK53_RS11250at 2512.443 kb on + strand, within LRK53_RS11250at 2512.443 kb on + strand, within LRK53_RS11250at 2512.443 kb on + strand, within LRK53_RS11250at 2512.443 kb on + strand, within LRK53_RS11250at 2512.444 kb on - strand, within LRK53_RS11250at 2512.444 kb on - strand, within LRK53_RS11250at 2512.444 kb on - strand, within LRK53_RS11250at 2512.444 kb on - strand, within LRK53_RS11250at 2512.444 kb on - strand, within LRK53_RS11250at 2512.444 kb on - strand, within LRK53_RS11250at 2512.467 kb on + strand, within LRK53_RS11250at 2512.467 kb on + strand, within LRK53_RS11250at 2512.467 kb on + strand, within LRK53_RS11250at 2512.468 kb on - strand, within LRK53_RS11250at 2512.468 kb on - strand, within LRK53_RS11250at 2512.468 kb on - strand, within LRK53_RS11250at 2512.468 kb on - strand, within LRK53_RS11250at 2512.468 kb on - strand, within LRK53_RS11250at 2512.715 kb on + strandat 2512.716 kb on - strandat 2512.716 kb on - strandat 2512.991 kb on + strand, within LRK53_RS11255at 2512.992 kb on - strand, within LRK53_RS11255at 2513.123 kb on + strand, within LRK53_RS11255at 2513.208 kb on + strand, within LRK53_RS11255at 2513.499 kb on + strand, within LRK53_RS11260at 2513.512 kb on + strand, within LRK53_RS11260at 2513.512 kb on + strand, within LRK53_RS11260at 2513.513 kb on - strand, within LRK53_RS11260at 2513.513 kb on - strand, within LRK53_RS11260at 2513.513 kb on - strand, within LRK53_RS11260at 2513.513 kb on - strand, within LRK53_RS11260at 2513.526 kb on + strand, within LRK53_RS11260at 2513.526 kb on + strand, within LRK53_RS11260at 2513.527 kb on - strand, within LRK53_RS11260at 2513.527 kb on - strand, within LRK53_RS11260at 2513.527 kb on - strand, within LRK53_RS11260at 2513.820 kb on + strand, within LRK53_RS11260at 2513.820 kb on + strand, within LRK53_RS11260at 2513.821 kb on - strand, within LRK53_RS11260at 2513.821 kb on - strand, within LRK53_RS11260at 2513.821 kb on - strand, within LRK53_RS11260at 2513.821 kb on - strand, within LRK53_RS11260at 2513.821 kb on - strand, within LRK53_RS11260at 2513.821 kb on - strand, within LRK53_RS11260at 2513.866 kb on + strand, within LRK53_RS11260at 2513.866 kb on + strand, within LRK53_RS11260at 2513.867 kb on - strand, within LRK53_RS11260at 2513.973 kb on + strandat 2513.973 kb on + strandat 2513.974 kb on - strandat 2513.974 kb on - strandat 2513.974 kb on - strandat 2513.974 kb on - strandat 2514.041 kb on + strand, within LRK53_RS11265at 2514.041 kb on + strand, within LRK53_RS11265at 2514.041 kb on + strand, within LRK53_RS11265at 2514.042 kb on - strand, within LRK53_RS11265at 2514.042 kb on - strand, within LRK53_RS11265at 2514.042 kb on - strand, within LRK53_RS11265at 2514.221 kb on - strandat 2514.339 kb on + strandat 2514.339 kb on + strandat 2514.340 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
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2,511,788 + LRK53_RS11245 0.78 +0.3
2,511,788 + LRK53_RS11245 0.78 -0.7
2,511,789 - LRK53_RS11245 0.78 -1.1
2,511,789 - LRK53_RS11245 0.78 -1.1
2,511,789 - LRK53_RS11245 0.78 -0.6
2,511,832 + LRK53_RS11245 0.82 -0.5
2,511,833 - LRK53_RS11245 0.83 -0.4
2,511,833 - LRK53_RS11245 0.83 -1.2
2,511,833 - LRK53_RS11245 0.83 -0.1
2,512,068 + +0.9
2,512,068 + +1.4
2,512,068 + +0.9
2,512,068 + -1.1
2,512,068 + -0.5
2,512,069 - -0.7
2,512,069 - +0.4
2,512,069 - -3.3
2,512,167 + LRK53_RS11250 0.20 -0.7
2,512,167 + LRK53_RS11250 0.20 -0.1
2,512,167 + LRK53_RS11250 0.20 -1.1
2,512,167 + LRK53_RS11250 0.20 -0.7
2,512,167 + LRK53_RS11250 0.20 +0.1
2,512,167 + LRK53_RS11250 0.20 +0.9
2,512,167 + LRK53_RS11250 0.20 -1.5
2,512,167 + LRK53_RS11250 0.20 +0.9
2,512,167 + LRK53_RS11250 0.20 -0.9
2,512,167 + LRK53_RS11250 0.20 -0.7
2,512,167 + LRK53_RS11250 0.20 +0.3
2,512,167 + LRK53_RS11250 0.20 -1.3
2,512,167 + LRK53_RS11250 0.20 -0.4
2,512,167 + LRK53_RS11250 0.20 -1.4
2,512,167 + LRK53_RS11250 0.20 -0.1
2,512,167 + LRK53_RS11250 0.20 -0.3
2,512,167 + LRK53_RS11250 0.20 +0.9
2,512,167 + LRK53_RS11250 0.20 -0.1
2,512,167 + LRK53_RS11250 0.20 -1.5
2,512,167 + LRK53_RS11250 0.20 -0.5
2,512,167 + LRK53_RS11250 0.20 -0.2
2,512,167 + LRK53_RS11250 0.20 +0.9
2,512,167 + LRK53_RS11250 0.20 -0.3
2,512,167 + LRK53_RS11250 0.20 +0.9
2,512,167 + LRK53_RS11250 0.20 +0.6
2,512,167 + LRK53_RS11250 0.20 +0.9
2,512,167 + LRK53_RS11250 0.20 +1.9
2,512,167 + LRK53_RS11250 0.20 -1.7
2,512,167 + LRK53_RS11250 0.20 -0.1
2,512,167 + LRK53_RS11250 0.20 +0.9
2,512,167 + LRK53_RS11250 0.20 +0.9
2,512,168 - LRK53_RS11250 0.21 -1.1
2,512,168 - LRK53_RS11250 0.21 +1.4
2,512,168 - LRK53_RS11250 0.21 -0.5
2,512,168 - LRK53_RS11250 0.21 -0.7
2,512,168 - LRK53_RS11250 0.21 -1.2
2,512,168 - LRK53_RS11250 0.21 -0.6
2,512,168 - LRK53_RS11250 0.21 -1.3
2,512,168 - LRK53_RS11250 0.21 +1.3
2,512,168 - LRK53_RS11250 0.21 -0.9
2,512,168 - LRK53_RS11250 0.21 -2.6
2,512,168 - LRK53_RS11250 0.21 -0.1
2,512,168 - LRK53_RS11250 0.21 -1.7
2,512,168 - LRK53_RS11250 0.21 +0.3
2,512,168 - LRK53_RS11250 0.21 -0.3
2,512,168 - LRK53_RS11250 0.21 +0.9
2,512,168 - LRK53_RS11250 0.21 +0.9
2,512,168 - LRK53_RS11250 0.21 -1.7
2,512,168 - LRK53_RS11250 0.21 -1.1
2,512,168 - LRK53_RS11250 0.21 -0.7
2,512,168 - LRK53_RS11250 0.21 -0.1
2,512,168 - LRK53_RS11250 0.21 -0.1
2,512,168 - LRK53_RS11250 0.21 +0.9
2,512,233 + LRK53_RS11250 0.30 -0.1
2,512,234 - LRK53_RS11250 0.30 -0.1
2,512,234 - LRK53_RS11250 0.30 +0.6
2,512,239 + LRK53_RS11250 0.30 +1.3
2,512,239 + LRK53_RS11250 0.30 -0.6
2,512,290 + LRK53_RS11250 0.37 +0.3
2,512,290 + LRK53_RS11250 0.37 -1.1
2,512,290 + LRK53_RS11250 0.37 -1.1
2,512,290 + LRK53_RS11250 0.37 -0.7
2,512,290 + LRK53_RS11250 0.37 -0.6
2,512,428 + LRK53_RS11250 0.56 -0.8
2,512,428 + LRK53_RS11250 0.56 -0.6
2,512,429 - LRK53_RS11250 0.56 -1.7
2,512,429 - LRK53_RS11250 0.56 -0.7
2,512,443 + LRK53_RS11250 0.58 -1.1
2,512,443 + LRK53_RS11250 0.58 -0.1
2,512,443 + LRK53_RS11250 0.58 -0.0
2,512,443 + LRK53_RS11250 0.58 -0.1
2,512,443 + LRK53_RS11250 0.58 -0.5
2,512,444 - LRK53_RS11250 0.58 +0.3
2,512,444 - LRK53_RS11250 0.58 +0.9
2,512,444 - LRK53_RS11250 0.58 -0.7
2,512,444 - LRK53_RS11250 0.58 -0.1
2,512,444 - LRK53_RS11250 0.58 -1.9
2,512,444 - LRK53_RS11250 0.58 +0.9
2,512,467 + LRK53_RS11250 0.61 -0.9
2,512,467 + LRK53_RS11250 0.61 -0.1
2,512,467 + LRK53_RS11250 0.61 -0.1
2,512,468 - LRK53_RS11250 0.62 -0.1
2,512,468 - LRK53_RS11250 0.62 -0.2
2,512,468 - LRK53_RS11250 0.62 -1.1
2,512,468 - LRK53_RS11250 0.62 -1.1
2,512,468 - LRK53_RS11250 0.62 -0.1
2,512,715 + +0.9
2,512,716 - -0.0
2,512,716 - +0.1
2,512,991 + LRK53_RS11255 0.37 -0.2
2,512,992 - LRK53_RS11255 0.38 +0.3
2,513,123 + LRK53_RS11255 0.58 -0.0
2,513,208 + LRK53_RS11255 0.70 -0.1
2,513,499 + LRK53_RS11260 0.17 -1.1
2,513,512 + LRK53_RS11260 0.19 -0.6
2,513,512 + LRK53_RS11260 0.19 +0.5
2,513,513 - LRK53_RS11260 0.19 +0.9
2,513,513 - LRK53_RS11260 0.19 +0.9
2,513,513 - LRK53_RS11260 0.19 +0.6
2,513,513 - LRK53_RS11260 0.19 -1.2
2,513,526 + LRK53_RS11260 0.21 +0.9
2,513,526 + LRK53_RS11260 0.21 -0.0
2,513,527 - LRK53_RS11260 0.21 -0.6
2,513,527 - LRK53_RS11260 0.21 -0.7
2,513,527 - LRK53_RS11260 0.21 -1.1
2,513,820 + LRK53_RS11260 0.68 -0.9
2,513,820 + LRK53_RS11260 0.68 +0.9
2,513,821 - LRK53_RS11260 0.68 -0.9
2,513,821 - LRK53_RS11260 0.68 -0.1
2,513,821 - LRK53_RS11260 0.68 +0.9
2,513,821 - LRK53_RS11260 0.68 +0.5
2,513,821 - LRK53_RS11260 0.68 -0.5
2,513,821 - LRK53_RS11260 0.68 +0.9
2,513,866 + LRK53_RS11260 0.75 -1.7
2,513,866 + LRK53_RS11260 0.75 -1.1
2,513,867 - LRK53_RS11260 0.75 +0.1
2,513,973 + -1.5
2,513,973 + -1.9
2,513,974 - -1.1
2,513,974 - -0.4
2,513,974 - -1.1
2,513,974 - -1.8
2,514,041 + LRK53_RS11265 0.20 +0.1
2,514,041 + LRK53_RS11265 0.20 -0.7
2,514,041 + LRK53_RS11265 0.20 +0.4
2,514,042 - LRK53_RS11265 0.20 -1.9
2,514,042 - LRK53_RS11265 0.20 -1.1
2,514,042 - LRK53_RS11265 0.20 +0.3
2,514,221 - -0.5
2,514,339 + -3.9
2,514,339 + -0.5
2,514,340 - -1.1

Or see this region's nucleotide sequence