Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS10270 and LRK53_RS10275 are separated by 10 nucleotides LRK53_RS10275 and LRK53_RS10280 are separated by 13 nucleotides LRK53_RS10280 and LRK53_RS10285 are separated by 20 nucleotides LRK53_RS10285 and LRK53_RS10290 are separated by 10 nucleotides
LRK53_RS10270: LRK53_RS10270 - alpha/beta fold hydrolase, at 2,294,104 to 2,294,946
_RS10270
LRK53_RS10275: LRK53_RS10275 - 2-oxopent-4-enoate hydratase, at 2,294,957 to 2,295,739
_RS10275
LRK53_RS10280: LRK53_RS10280 - 2-oxo-3-hexenedioate decarboxylase, at 2,295,753 to 2,296,541
_RS10280
LRK53_RS10285: LRK53_RS10285 - acetaldehyde dehydrogenase (acetylating), at 2,296,562 to 2,297,467
_RS10285
LRK53_RS10290: LRK53_RS10290 - 4-hydroxy-2-oxovalerate aldolase, at 2,297,478 to 2,298,524
_RS10290
Position (kb)
2295
2296
2297 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2294.996 kb on + strand at 2294.996 kb on + strand at 2294.996 kb on + strand at 2294.997 kb on - strand at 2295.179 kb on + strand, within LRK53_RS10275 at 2295.179 kb on + strand, within LRK53_RS10275 at 2295.180 kb on - strand, within LRK53_RS10275 at 2295.218 kb on + strand, within LRK53_RS10275 at 2295.219 kb on - strand, within LRK53_RS10275 at 2295.219 kb on - strand, within LRK53_RS10275 at 2295.219 kb on - strand, within LRK53_RS10275 at 2295.219 kb on - strand, within LRK53_RS10275 at 2295.219 kb on - strand, within LRK53_RS10275 at 2295.219 kb on - strand, within LRK53_RS10275 at 2295.219 kb on - strand, within LRK53_RS10275 at 2295.354 kb on + strand, within LRK53_RS10275 at 2295.354 kb on + strand, within LRK53_RS10275 at 2295.355 kb on - strand, within LRK53_RS10275 at 2295.355 kb on - strand, within LRK53_RS10275 at 2295.444 kb on + strand, within LRK53_RS10275 at 2295.444 kb on + strand, within LRK53_RS10275 at 2295.444 kb on + strand, within LRK53_RS10275 at 2295.445 kb on - strand, within LRK53_RS10275 at 2295.445 kb on - strand, within LRK53_RS10275 at 2295.873 kb on + strand, within LRK53_RS10280 at 2295.874 kb on - strand, within LRK53_RS10280 at 2295.874 kb on - strand, within LRK53_RS10280 at 2296.012 kb on - strand, within LRK53_RS10280 at 2296.751 kb on + strand, within LRK53_RS10285 at 2296.751 kb on + strand, within LRK53_RS10285 at 2296.752 kb on - strand, within LRK53_RS10285 at 2296.752 kb on - strand, within LRK53_RS10285 at 2296.752 kb on - strand, within LRK53_RS10285 at 2296.752 kb on - strand, within LRK53_RS10285 at 2296.752 kb on - strand, within LRK53_RS10285 at 2296.770 kb on + strand, within LRK53_RS10285 at 2296.770 kb on + strand, within LRK53_RS10285 at 2296.771 kb on - strand, within LRK53_RS10285 at 2296.771 kb on - strand, within LRK53_RS10285 at 2296.880 kb on + strand, within LRK53_RS10285 at 2297.009 kb on + strand, within LRK53_RS10285 at 2297.009 kb on + strand, within LRK53_RS10285 at 2297.009 kb on + strand, within LRK53_RS10285 at 2297.010 kb on - strand, within LRK53_RS10285 at 2297.189 kb on + strand, within LRK53_RS10285 at 2297.189 kb on + strand, within LRK53_RS10285 at 2297.189 kb on + strand, within LRK53_RS10285 at 2297.189 kb on + strand, within LRK53_RS10285 at 2297.189 kb on + strand, within LRK53_RS10285 at 2297.189 kb on + strand, within LRK53_RS10285 at 2297.190 kb on - strand, within LRK53_RS10285 at 2297.190 kb on - strand, within LRK53_RS10285 at 2297.190 kb on - strand, within LRK53_RS10285 at 2297.190 kb on - strand, within LRK53_RS10285 at 2297.190 kb on - strand, within LRK53_RS10285 at 2297.267 kb on + strand, within LRK53_RS10285 at 2297.267 kb on + strand, within LRK53_RS10285 at 2297.268 kb on - strand, within LRK53_RS10285 at 2297.268 kb on - strand, within LRK53_RS10285 at 2297.268 kb on - strand, within LRK53_RS10285 at 2297.268 kb on - strand, within LRK53_RS10285 at 2297.268 kb on - strand, within LRK53_RS10285 at 2297.268 kb on - strand, within LRK53_RS10285 at 2297.268 kb on - strand, within LRK53_RS10285 at 2297.268 kb on - strand, within LRK53_RS10285 at 2297.268 kb on - strand, within LRK53_RS10285 at 2297.327 kb on + strand, within LRK53_RS10285 at 2297.366 kb on + strand, within LRK53_RS10285 at 2297.366 kb on + strand, within LRK53_RS10285 at 2297.367 kb on - strand, within LRK53_RS10285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 2,294,996 + +0.9 2,294,996 + -0.1 2,294,996 + -0.1 2,294,997 - -0.7 2,295,179 + LRK53_RS10275 0.28 +1.1 2,295,179 + LRK53_RS10275 0.28 -0.1 2,295,180 - LRK53_RS10275 0.28 -0.4 2,295,218 + LRK53_RS10275 0.33 -0.0 2,295,219 - LRK53_RS10275 0.33 +0.1 2,295,219 - LRK53_RS10275 0.33 -0.1 2,295,219 - LRK53_RS10275 0.33 +0.5 2,295,219 - LRK53_RS10275 0.33 +1.9 2,295,219 - LRK53_RS10275 0.33 +0.5 2,295,219 - LRK53_RS10275 0.33 +0.3 2,295,219 - LRK53_RS10275 0.33 -0.7 2,295,354 + LRK53_RS10275 0.51 -1.1 2,295,354 + LRK53_RS10275 0.51 +0.9 2,295,355 - LRK53_RS10275 0.51 +1.3 2,295,355 - LRK53_RS10275 0.51 +2.2 2,295,444 + LRK53_RS10275 0.62 -0.7 2,295,444 + LRK53_RS10275 0.62 -1.1 2,295,444 + LRK53_RS10275 0.62 -0.7 2,295,445 - LRK53_RS10275 0.62 -0.1 2,295,445 - LRK53_RS10275 0.62 +0.1 2,295,873 + LRK53_RS10280 0.15 -0.1 2,295,874 - LRK53_RS10280 0.15 -1.3 2,295,874 - LRK53_RS10280 0.15 +1.9 2,296,012 - LRK53_RS10280 0.33 +1.9 2,296,751 + LRK53_RS10285 0.21 -0.6 2,296,751 + LRK53_RS10285 0.21 -1.7 2,296,752 - LRK53_RS10285 0.21 -1.7 2,296,752 - LRK53_RS10285 0.21 -0.1 2,296,752 - LRK53_RS10285 0.21 -0.7 2,296,752 - LRK53_RS10285 0.21 -1.6 2,296,752 - LRK53_RS10285 0.21 -0.1 2,296,770 + LRK53_RS10285 0.23 +0.3 2,296,770 + LRK53_RS10285 0.23 -1.1 2,296,771 - LRK53_RS10285 0.23 -0.4 2,296,771 - LRK53_RS10285 0.23 +1.9 2,296,880 + LRK53_RS10285 0.35 -0.7 2,297,009 + LRK53_RS10285 0.49 +0.9 2,297,009 + LRK53_RS10285 0.49 -0.7 2,297,009 + LRK53_RS10285 0.49 +2.2 2,297,010 - LRK53_RS10285 0.49 -1.0 2,297,189 + LRK53_RS10285 0.69 +0.9 2,297,189 + LRK53_RS10285 0.69 -0.7 2,297,189 + LRK53_RS10285 0.69 -0.4 2,297,189 + LRK53_RS10285 0.69 -0.7 2,297,189 + LRK53_RS10285 0.69 -1.2 2,297,189 + LRK53_RS10285 0.69 -0.7 2,297,190 - LRK53_RS10285 0.69 +1.1 2,297,190 - LRK53_RS10285 0.69 -0.3 2,297,190 - LRK53_RS10285 0.69 +0.9 2,297,190 - LRK53_RS10285 0.69 -0.7 2,297,190 - LRK53_RS10285 0.69 -0.6 2,297,267 + LRK53_RS10285 0.78 -0.1 2,297,267 + LRK53_RS10285 0.78 -0.4 2,297,268 - LRK53_RS10285 0.78 -0.3 2,297,268 - LRK53_RS10285 0.78 +2.5 2,297,268 - LRK53_RS10285 0.78 +0.5 2,297,268 - LRK53_RS10285 0.78 -0.9 2,297,268 - LRK53_RS10285 0.78 -1.1 2,297,268 - LRK53_RS10285 0.78 +0.1 2,297,268 - LRK53_RS10285 0.78 -0.7 2,297,268 - LRK53_RS10285 0.78 +0.9 2,297,268 - LRK53_RS10285 0.78 -1.1 2,297,327 + LRK53_RS10285 0.84 -0.9 2,297,366 + LRK53_RS10285 0.89 +0.3 2,297,366 + LRK53_RS10285 0.89 -2.3 2,297,367 - LRK53_RS10285 0.89 +0.9
Or see this region's nucleotide sequence