Strain Fitness in Rhodanobacter sp000427505 FW510-R12 around LRK53_RS10275

Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntLRK53_RS10265 and LRK53_RS10270 are separated by 20 nucleotidesLRK53_RS10270 and LRK53_RS10275 are separated by 10 nucleotidesLRK53_RS10275 and LRK53_RS10280 are separated by 13 nucleotidesLRK53_RS10280 and LRK53_RS10285 are separated by 20 nucleotides LRK53_RS10265: LRK53_RS10265 - 2-hydroxymuconic semialdehyde dehydrogenase, at 2,292,626 to 2,294,083 _RS10265 LRK53_RS10270: LRK53_RS10270 - alpha/beta fold hydrolase, at 2,294,104 to 2,294,946 _RS10270 LRK53_RS10275: LRK53_RS10275 - 2-oxopent-4-enoate hydratase, at 2,294,957 to 2,295,739 _RS10275 LRK53_RS10280: LRK53_RS10280 - 2-oxo-3-hexenedioate decarboxylase, at 2,295,753 to 2,296,541 _RS10280 LRK53_RS10285: LRK53_RS10285 - acetaldehyde dehydrogenase (acetylating), at 2,296,562 to 2,297,467 _RS10285 Position (kb) 2294 2295 2296Strain fitness (log2 ratio) -2 -1 0 1 2at 2294.045 kb on + strandat 2294.045 kb on + strandat 2294.045 kb on + strandat 2294.045 kb on + strandat 2294.046 kb on - strandat 2294.046 kb on - strandat 2294.075 kb on + strandat 2294.075 kb on + strandat 2294.075 kb on + strandat 2294.076 kb on - strandat 2294.076 kb on - strandat 2294.167 kb on + strandat 2294.167 kb on + strandat 2294.167 kb on + strandat 2294.167 kb on + strandat 2294.167 kb on + strandat 2294.168 kb on - strandat 2294.168 kb on - strandat 2294.168 kb on - strandat 2294.168 kb on - strandat 2294.168 kb on - strandat 2294.347 kb on + strand, within LRK53_RS10270at 2294.348 kb on - strand, within LRK53_RS10270at 2294.555 kb on - strand, within LRK53_RS10270at 2294.602 kb on + strand, within LRK53_RS10270at 2294.996 kb on + strandat 2294.996 kb on + strandat 2294.996 kb on + strandat 2294.997 kb on - strandat 2295.179 kb on + strand, within LRK53_RS10275at 2295.179 kb on + strand, within LRK53_RS10275at 2295.180 kb on - strand, within LRK53_RS10275at 2295.218 kb on + strand, within LRK53_RS10275at 2295.219 kb on - strand, within LRK53_RS10275at 2295.219 kb on - strand, within LRK53_RS10275at 2295.219 kb on - strand, within LRK53_RS10275at 2295.219 kb on - strand, within LRK53_RS10275at 2295.219 kb on - strand, within LRK53_RS10275at 2295.219 kb on - strand, within LRK53_RS10275at 2295.219 kb on - strand, within LRK53_RS10275at 2295.354 kb on + strand, within LRK53_RS10275at 2295.354 kb on + strand, within LRK53_RS10275at 2295.355 kb on - strand, within LRK53_RS10275at 2295.355 kb on - strand, within LRK53_RS10275at 2295.444 kb on + strand, within LRK53_RS10275at 2295.444 kb on + strand, within LRK53_RS10275at 2295.444 kb on + strand, within LRK53_RS10275at 2295.445 kb on - strand, within LRK53_RS10275at 2295.445 kb on - strand, within LRK53_RS10275at 2295.873 kb on + strand, within LRK53_RS10280at 2295.874 kb on - strand, within LRK53_RS10280at 2295.874 kb on - strand, within LRK53_RS10280at 2296.012 kb on - strand, within LRK53_RS10280

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
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2,294,045 + -0.1
2,294,045 + -1.5
2,294,045 + -1.1
2,294,045 + +0.9
2,294,046 - +0.1
2,294,046 - -1.5
2,294,075 + +0.9
2,294,075 + +0.1
2,294,075 + +0.9
2,294,076 - -0.1
2,294,076 - -0.1
2,294,167 + +0.9
2,294,167 + -1.2
2,294,167 + -0.1
2,294,167 + -1.5
2,294,167 + -0.1
2,294,168 - +1.3
2,294,168 - -1.1
2,294,168 - -1.1
2,294,168 - +0.9
2,294,168 - -0.1
2,294,347 + LRK53_RS10270 0.29 +0.1
2,294,348 - LRK53_RS10270 0.29 -0.4
2,294,555 - LRK53_RS10270 0.53 -2.5
2,294,602 + LRK53_RS10270 0.59 +0.1
2,294,996 + +0.9
2,294,996 + -0.1
2,294,996 + -0.1
2,294,997 - -0.7
2,295,179 + LRK53_RS10275 0.28 +1.1
2,295,179 + LRK53_RS10275 0.28 -0.1
2,295,180 - LRK53_RS10275 0.28 -0.4
2,295,218 + LRK53_RS10275 0.33 -0.0
2,295,219 - LRK53_RS10275 0.33 +0.1
2,295,219 - LRK53_RS10275 0.33 -0.1
2,295,219 - LRK53_RS10275 0.33 +0.5
2,295,219 - LRK53_RS10275 0.33 +1.9
2,295,219 - LRK53_RS10275 0.33 +0.5
2,295,219 - LRK53_RS10275 0.33 +0.3
2,295,219 - LRK53_RS10275 0.33 -0.7
2,295,354 + LRK53_RS10275 0.51 -1.1
2,295,354 + LRK53_RS10275 0.51 +0.9
2,295,355 - LRK53_RS10275 0.51 +1.3
2,295,355 - LRK53_RS10275 0.51 +2.2
2,295,444 + LRK53_RS10275 0.62 -0.7
2,295,444 + LRK53_RS10275 0.62 -1.1
2,295,444 + LRK53_RS10275 0.62 -0.7
2,295,445 - LRK53_RS10275 0.62 -0.1
2,295,445 - LRK53_RS10275 0.62 +0.1
2,295,873 + LRK53_RS10280 0.15 -0.1
2,295,874 - LRK53_RS10280 0.15 -1.3
2,295,874 - LRK53_RS10280 0.15 +1.9
2,296,012 - LRK53_RS10280 0.33 +1.9

Or see this region's nucleotide sequence