Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS09445 and LRK53_RS09450 are separated by 130 nucleotides LRK53_RS09450 and LRK53_RS09455 are separated by 182 nucleotides LRK53_RS09455 and LRK53_RS09460 are separated by 163 nucleotides
LRK53_RS09445: LRK53_RS09445 - alkyl hydroperoxide reductase subunit F, at 2,118,936 to 2,120,522
_RS09445
LRK53_RS09450: LRK53_RS09450 - alkyl hydroperoxide reductase subunit C, at 2,120,653 to 2,121,216
_RS09450
LRK53_RS09455: LRK53_RS09455 - dicarboxylate/amino acid:cation symporter, at 2,121,399 to 2,122,715
_RS09455
LRK53_RS09460: LRK53_RS09460 - methylisocitrate lyase, at 2,122,879 to 2,123,763
_RS09460
Position (kb)
2121
2122
2123 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2120.485 kb on + strand at 2120.485 kb on + strand at 2120.485 kb on + strand at 2120.485 kb on + strand at 2120.485 kb on + strand at 2120.485 kb on + strand at 2120.485 kb on + strand at 2120.486 kb on - strand at 2120.486 kb on - strand at 2120.552 kb on + strand at 2120.553 kb on - strand at 2120.554 kb on - strand at 2120.586 kb on - strand at 2120.586 kb on - strand at 2120.654 kb on + strand at 2120.654 kb on + strand at 2121.258 kb on + strand at 2121.258 kb on + strand at 2121.258 kb on + strand at 2121.259 kb on - strand at 2121.259 kb on - strand at 2121.259 kb on - strand at 2121.259 kb on - strand at 2121.281 kb on + strand at 2121.314 kb on - strand at 2121.314 kb on - strand at 2121.314 kb on - strand at 2121.318 kb on - strand at 2121.318 kb on - strand at 2121.331 kb on + strand at 2121.331 kb on + strand at 2121.331 kb on + strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.400 kb on + strand at 2121.434 kb on - strand at 2121.434 kb on - strand at 2121.877 kb on + strand, within LRK53_RS09455 at 2121.877 kb on + strand, within LRK53_RS09455 at 2121.877 kb on + strand, within LRK53_RS09455 at 2121.878 kb on - strand, within LRK53_RS09455 at 2121.878 kb on - strand, within LRK53_RS09455 at 2121.878 kb on - strand, within LRK53_RS09455 at 2121.878 kb on - strand, within LRK53_RS09455 at 2122.021 kb on + strand, within LRK53_RS09455 at 2122.021 kb on + strand, within LRK53_RS09455 at 2122.021 kb on + strand, within LRK53_RS09455 at 2122.021 kb on + strand, within LRK53_RS09455 at 2122.021 kb on + strand, within LRK53_RS09455 at 2122.022 kb on - strand, within LRK53_RS09455 at 2122.022 kb on - strand, within LRK53_RS09455 at 2122.022 kb on - strand, within LRK53_RS09455 at 2122.022 kb on - strand, within LRK53_RS09455 at 2122.022 kb on - strand, within LRK53_RS09455 at 2122.022 kb on - strand, within LRK53_RS09455 at 2122.022 kb on - strand, within LRK53_RS09455 at 2122.022 kb on - strand, within LRK53_RS09455 at 2122.128 kb on + strand, within LRK53_RS09455 at 2122.128 kb on + strand, within LRK53_RS09455 at 2122.129 kb on - strand, within LRK53_RS09455 at 2122.129 kb on - strand, within LRK53_RS09455 at 2122.327 kb on + strand, within LRK53_RS09455 at 2122.328 kb on - strand, within LRK53_RS09455 at 2122.328 kb on - strand, within LRK53_RS09455 at 2122.328 kb on - strand, within LRK53_RS09455 at 2122.328 kb on - strand, within LRK53_RS09455 at 2122.328 kb on - strand, within LRK53_RS09455 at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.709 kb on + strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.710 kb on - strand at 2122.727 kb on + strand at 2122.727 kb on + strand at 2122.727 kb on + strand at 2122.727 kb on + strand at 2122.727 kb on + strand at 2122.728 kb on - strand at 2122.728 kb on - strand at 2122.728 kb on - strand at 2122.852 kb on + strand at 2122.878 kb on + strand at 2122.878 kb on + strand at 2122.878 kb on + strand at 2122.878 kb on + strand at 2122.878 kb on + strand at 2122.878 kb on + strand at 2122.879 kb on - strand at 2122.879 kb on - strand at 2122.879 kb on - strand at 2122.879 kb on - strand at 2122.966 kb on + strand at 2122.967 kb on - strand at 2123.007 kb on - strand, within LRK53_RS09460 at 2123.008 kb on + strand, within LRK53_RS09460 at 2123.008 kb on + strand, within LRK53_RS09460 at 2123.008 kb on + strand, within LRK53_RS09460 at 2123.008 kb on + strand, within LRK53_RS09460 at 2123.009 kb on - strand, within LRK53_RS09460 at 2123.009 kb on - strand, within LRK53_RS09460 at 2123.009 kb on - strand, within LRK53_RS09460 at 2123.009 kb on - strand, within LRK53_RS09460 at 2123.009 kb on - strand, within LRK53_RS09460 at 2123.009 kb on - strand, within LRK53_RS09460 at 2123.009 kb on - strand, within LRK53_RS09460 at 2123.009 kb on - strand, within LRK53_RS09460 at 2123.407 kb on + strand, within LRK53_RS09460 at 2123.407 kb on + strand, within LRK53_RS09460 at 2123.407 kb on + strand, within LRK53_RS09460 at 2123.408 kb on - strand, within LRK53_RS09460 at 2123.581 kb on + strand, within LRK53_RS09460 at 2123.581 kb on + strand, within LRK53_RS09460 at 2123.581 kb on + strand, within LRK53_RS09460 at 2123.581 kb on + strand, within LRK53_RS09460 at 2123.581 kb on + strand, within LRK53_RS09460 at 2123.582 kb on - strand, within LRK53_RS09460 at 2123.652 kb on + strand, within LRK53_RS09460 at 2123.653 kb on - strand, within LRK53_RS09460 at 2123.698 kb on + strand at 2123.698 kb on + strand at 2123.698 kb on + strand at 2123.698 kb on + strand at 2123.699 kb on - strand at 2123.699 kb on - strand at 2123.699 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 2,120,485 + +0.1 2,120,485 + -2.9 2,120,485 + -1.7 2,120,485 + -1.3 2,120,485 + -1.5 2,120,485 + +0.5 2,120,485 + +0.9 2,120,486 - -0.7 2,120,486 - -0.5 2,120,552 + +0.9 2,120,553 - -0.7 2,120,554 - -0.1 2,120,586 - +0.6 2,120,586 - -1.1 2,120,654 + -0.7 2,120,654 + -0.7 2,121,258 + +2.5 2,121,258 + -1.1 2,121,258 + -0.1 2,121,259 - -0.9 2,121,259 - +1.9 2,121,259 - +0.2 2,121,259 - +0.6 2,121,281 + -0.7 2,121,314 - +0.6 2,121,314 - -0.4 2,121,314 - -0.9 2,121,318 - -0.9 2,121,318 - -0.4 2,121,331 + -0.1 2,121,331 + +0.9 2,121,331 + +1.9 2,121,332 - +0.3 2,121,332 - +0.2 2,121,332 - -0.4 2,121,332 - +0.5 2,121,332 - -0.1 2,121,332 - -0.0 2,121,332 - -1.1 2,121,332 - -0.7 2,121,332 - -0.3 2,121,400 + +0.2 2,121,434 - +1.5 2,121,434 - -1.1 2,121,877 + LRK53_RS09455 0.36 -0.1 2,121,877 + LRK53_RS09455 0.36 -0.9 2,121,877 + LRK53_RS09455 0.36 +1.9 2,121,878 - LRK53_RS09455 0.36 +0.9 2,121,878 - LRK53_RS09455 0.36 -0.3 2,121,878 - LRK53_RS09455 0.36 +0.3 2,121,878 - LRK53_RS09455 0.36 -1.1 2,122,021 + LRK53_RS09455 0.47 +0.4 2,122,021 + LRK53_RS09455 0.47 +0.2 2,122,021 + LRK53_RS09455 0.47 +1.3 2,122,021 + LRK53_RS09455 0.47 +1.2 2,122,021 + LRK53_RS09455 0.47 -0.7 2,122,022 - LRK53_RS09455 0.47 -1.1 2,122,022 - LRK53_RS09455 0.47 -0.7 2,122,022 - LRK53_RS09455 0.47 -1.1 2,122,022 - LRK53_RS09455 0.47 -0.7 2,122,022 - LRK53_RS09455 0.47 -0.1 2,122,022 - LRK53_RS09455 0.47 +0.3 2,122,022 - LRK53_RS09455 0.47 -0.3 2,122,022 - LRK53_RS09455 0.47 +2.5 2,122,128 + LRK53_RS09455 0.55 -0.3 2,122,128 + LRK53_RS09455 0.55 -1.7 2,122,129 - LRK53_RS09455 0.55 +0.0 2,122,129 - LRK53_RS09455 0.55 -0.9 2,122,327 + LRK53_RS09455 0.70 +1.5 2,122,328 - LRK53_RS09455 0.71 -1.0 2,122,328 - LRK53_RS09455 0.71 -1.4 2,122,328 - LRK53_RS09455 0.71 +0.1 2,122,328 - LRK53_RS09455 0.71 -1.1 2,122,328 - LRK53_RS09455 0.71 +0.9 2,122,709 + +1.9 2,122,709 + -1.7 2,122,709 + -0.7 2,122,709 + -1.3 2,122,709 + +0.9 2,122,709 + +0.9 2,122,709 + +0.3 2,122,709 + -0.1 2,122,709 + -1.2 2,122,709 + -0.6 2,122,709 + +0.4 2,122,709 + -0.2 2,122,709 + +0.1 2,122,710 - +1.3 2,122,710 - -1.1 2,122,710 - +0.9 2,122,710 - +0.5 2,122,710 - -1.0 2,122,710 - -1.4 2,122,710 - -0.4 2,122,710 - +0.9 2,122,710 - +0.5 2,122,710 - +0.3 2,122,710 - -1.1 2,122,710 - +1.3 2,122,710 - -0.7 2,122,710 - -1.7 2,122,710 - +1.5 2,122,710 - +0.7 2,122,710 - -0.7 2,122,710 - -1.0 2,122,710 - -0.8 2,122,710 - +0.1 2,122,710 - +1.9 2,122,727 + +0.9 2,122,727 + -0.7 2,122,727 + +1.9 2,122,727 + -1.1 2,122,727 + +0.9 2,122,728 - -0.1 2,122,728 - -0.1 2,122,728 - +1.3 2,122,852 + -0.1 2,122,878 + +0.6 2,122,878 + -0.1 2,122,878 + -0.1 2,122,878 + +0.9 2,122,878 + +1.5 2,122,878 + +1.5 2,122,879 - -0.7 2,122,879 - +0.9 2,122,879 - -0.9 2,122,879 - +1.5 2,122,966 + +0.2 2,122,967 - +0.3 2,123,007 - LRK53_RS09460 0.14 -1.7 2,123,008 + LRK53_RS09460 0.15 -0.4 2,123,008 + LRK53_RS09460 0.15 -0.1 2,123,008 + LRK53_RS09460 0.15 -1.9 2,123,008 + LRK53_RS09460 0.15 +1.9 2,123,009 - LRK53_RS09460 0.15 -1.4 2,123,009 - LRK53_RS09460 0.15 +0.9 2,123,009 - LRK53_RS09460 0.15 -0.1 2,123,009 - LRK53_RS09460 0.15 -0.8 2,123,009 - LRK53_RS09460 0.15 -0.1 2,123,009 - LRK53_RS09460 0.15 +0.9 2,123,009 - LRK53_RS09460 0.15 -0.1 2,123,009 - LRK53_RS09460 0.15 +0.9 2,123,407 + LRK53_RS09460 0.60 -1.1 2,123,407 + LRK53_RS09460 0.60 +0.9 2,123,407 + LRK53_RS09460 0.60 -0.1 2,123,408 - LRK53_RS09460 0.60 +0.3 2,123,581 + LRK53_RS09460 0.79 -0.5 2,123,581 + LRK53_RS09460 0.79 -0.1 2,123,581 + LRK53_RS09460 0.79 +0.9 2,123,581 + LRK53_RS09460 0.79 +0.3 2,123,581 + LRK53_RS09460 0.79 +0.3 2,123,582 - LRK53_RS09460 0.79 +0.9 2,123,652 + LRK53_RS09460 0.87 -0.7 2,123,653 - LRK53_RS09460 0.87 -1.1 2,123,698 + -0.7 2,123,698 + -0.7 2,123,698 + +3.2 2,123,698 + -0.4 2,123,699 - -0.3 2,123,699 - +0.1 2,123,699 - -1.1
Or see this region's nucleotide sequence