Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08445 and LRK53_RS08450 are separated by 699 nucleotides LRK53_RS08450 and LRK53_RS08455 overlap by 4 nucleotides LRK53_RS08455 and LRK53_RS08460 are separated by 28 nucleotides
LRK53_RS08445: LRK53_RS08445 - ribonucleoside-diphosphate reductase subunit alpha, at 1,896,353 to 1,898,776
_RS08445
LRK53_RS08450: LRK53_RS08450 - YerC/YecD family TrpR-related protein, at 1,899,476 to 1,899,808
_RS08450
LRK53_RS08455: LRK53_RS08455 - ATP phosphoribosyltransferase, at 1,899,805 to 1,900,698
_RS08455
LRK53_RS08460: LRK53_RS08460 - histidinol dehydrogenase, at 1,900,727 to 1,902,025
_RS08460
Position (kb)
1899
1900 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1899.184 kb on - strand at 1899.184 kb on - strand at 1899.184 kb on - strand at 1899.184 kb on - strand at 1899.184 kb on - strand at 1899.225 kb on - strand at 1899.356 kb on + strand at 1899.439 kb on + strand at 1899.439 kb on + strand at 1899.452 kb on - strand at 1899.457 kb on - strand at 1899.485 kb on - strand at 1899.530 kb on + strand, within LRK53_RS08450 at 1899.530 kb on + strand, within LRK53_RS08450 at 1899.531 kb on - strand, within LRK53_RS08450 at 1899.531 kb on - strand, within LRK53_RS08450 at 1899.531 kb on - strand, within LRK53_RS08450 at 1899.531 kb on - strand, within LRK53_RS08450 at 1899.531 kb on - strand, within LRK53_RS08450 at 1899.723 kb on - strand, within LRK53_RS08450 at 1899.747 kb on - strand, within LRK53_RS08450 at 1900.144 kb on + strand, within LRK53_RS08455 at 1900.144 kb on + strand, within LRK53_RS08455 at 1900.144 kb on + strand, within LRK53_RS08455 at 1900.145 kb on - strand, within LRK53_RS08455 at 1900.145 kb on - strand, within LRK53_RS08455 at 1900.145 kb on - strand, within LRK53_RS08455 at 1900.177 kb on - strand, within LRK53_RS08455 at 1900.190 kb on - strand, within LRK53_RS08455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 1,899,184 - -0.7 1,899,184 - -2.2 1,899,184 - +0.9 1,899,184 - +0.9 1,899,184 - -0.1 1,899,225 - +0.9 1,899,356 + +0.9 1,899,439 + +0.9 1,899,439 + +0.9 1,899,452 - +0.5 1,899,457 - +0.9 1,899,485 - +1.6 1,899,530 + LRK53_RS08450 0.16 +0.6 1,899,530 + LRK53_RS08450 0.16 +0.3 1,899,531 - LRK53_RS08450 0.17 +0.9 1,899,531 - LRK53_RS08450 0.17 -1.7 1,899,531 - LRK53_RS08450 0.17 +0.3 1,899,531 - LRK53_RS08450 0.17 +0.2 1,899,531 - LRK53_RS08450 0.17 -0.2 1,899,723 - LRK53_RS08450 0.74 +1.9 1,899,747 - LRK53_RS08450 0.81 +0.4 1,900,144 + LRK53_RS08455 0.38 +0.6 1,900,144 + LRK53_RS08455 0.38 +0.1 1,900,144 + LRK53_RS08455 0.38 -0.7 1,900,145 - LRK53_RS08455 0.38 -0.1 1,900,145 - LRK53_RS08455 0.38 +0.9 1,900,145 - LRK53_RS08455 0.38 -1.4 1,900,177 - LRK53_RS08455 0.42 -1.1 1,900,190 - LRK53_RS08455 0.43 -0.5
Or see this region's nucleotide sequence