Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07950 and LRK53_RS07955 are separated by 166 nucleotides LRK53_RS07955 and LRK53_RS07960 are separated by 58 nucleotides
LRK53_RS07950: LRK53_RS07950 - NAD kinase, at 1,785,176 to 1,785,937
_RS07950
LRK53_RS07955: LRK53_RS07955 - 5'-nucleotidase, at 1,786,104 to 1,787,033
_RS07955
LRK53_RS07960: LRK53_RS07960 - carboxylesterase family protein, at 1,787,092 to 1,788,771
_RS07960
Position (kb) 1786
1787
1788 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 1785.116 kb on + strand at 1785.117 kb on - strand at 1785.126 kb on + strand at 1785.126 kb on + strand at 1785.126 kb on + strand at 1785.126 kb on + strand at 1785.127 kb on - strand at 1785.127 kb on - strand at 1785.140 kb on + strand at 1785.141 kb on - strand at 1785.141 kb on - strand at 1785.141 kb on - strand at 1785.141 kb on - strand at 1785.146 kb on + strand at 1785.146 kb on + strand at 1785.146 kb on + strand at 1785.146 kb on + strand at 1785.146 kb on + strand at 1785.146 kb on + strand at 1785.146 kb on + strand at 1785.146 kb on + strand at 1785.146 kb on + strand at 1785.146 kb on + strand at 1785.147 kb on - strand at 1785.951 kb on + strand at 1785.951 kb on + strand at 1785.951 kb on + strand at 1785.952 kb on - strand at 1785.952 kb on - strand at 1786.044 kb on + strand at 1786.044 kb on + strand at 1786.044 kb on + strand at 1786.044 kb on + strand at 1786.044 kb on + strand at 1786.045 kb on - strand at 1786.070 kb on + strand at 1786.070 kb on + strand at 1786.070 kb on + strand at 1786.071 kb on - strand at 1786.071 kb on - strand at 1786.071 kb on - strand at 1786.071 kb on - strand at 1786.071 kb on - strand at 1786.071 kb on - strand at 1786.071 kb on - strand at 1786.115 kb on - strand at 1786.115 kb on - strand at 1786.115 kb on - strand at 1786.115 kb on - strand at 1786.115 kb on - strand at 1786.115 kb on - strand at 1786.115 kb on - strand at 1786.419 kb on + strand, within LRK53_RS07955 at 1786.419 kb on + strand, within LRK53_RS07955 at 1786.420 kb on - strand, within LRK53_RS07955 at 1786.420 kb on - strand, within LRK53_RS07955 at 1786.420 kb on - strand, within LRK53_RS07955 at 1786.420 kb on - strand, within LRK53_RS07955 at 1786.473 kb on + strand, within LRK53_RS07955 at 1786.474 kb on - strand, within LRK53_RS07955 at 1786.474 kb on - strand, within LRK53_RS07955 at 1786.474 kb on - strand, within LRK53_RS07955 at 1786.474 kb on - strand, within LRK53_RS07955 at 1786.474 kb on - strand, within LRK53_RS07955 at 1786.474 kb on - strand, within LRK53_RS07955 at 1787.068 kb on + strand at 1787.068 kb on + strand at 1787.068 kb on + strand at 1787.069 kb on - strand at 1787.069 kb on - strand at 1787.069 kb on - strand at 1787.069 kb on - strand at 1787.069 kb on - strand at 1787.201 kb on + strand at 1787.201 kb on + strand at 1787.201 kb on + strand at 1787.202 kb on - strand at 1787.202 kb on - strand at 1787.202 kb on - strand at 1787.202 kb on - strand at 1787.202 kb on - strand at 1787.202 kb on - strand at 1787.254 kb on + strand at 1787.254 kb on + strand at 1787.254 kb on + strand at 1787.254 kb on + strand at 1787.254 kb on + strand at 1787.255 kb on - strand at 1787.255 kb on - strand at 1787.255 kb on - strand at 1787.255 kb on - strand at 1787.383 kb on + strand, within LRK53_RS07960 at 1787.383 kb on + strand, within LRK53_RS07960 at 1787.383 kb on + strand, within LRK53_RS07960 at 1787.383 kb on + strand, within LRK53_RS07960 at 1787.383 kb on + strand, within LRK53_RS07960 at 1787.383 kb on + strand, within LRK53_RS07960 at 1787.383 kb on + strand, within LRK53_RS07960 at 1787.383 kb on + strand, within LRK53_RS07960 at 1787.383 kb on + strand, within LRK53_RS07960 at 1787.383 kb on + strand, within LRK53_RS07960 at 1787.383 kb on + strand, within LRK53_RS07960 at 1787.384 kb on - strand, within LRK53_RS07960 at 1787.384 kb on - strand, within LRK53_RS07960 at 1787.384 kb on - strand, within LRK53_RS07960 at 1787.384 kb on - strand, within LRK53_RS07960 at 1787.384 kb on - strand, within LRK53_RS07960 at 1787.384 kb on - strand, within LRK53_RS07960 at 1787.384 kb on - strand, within LRK53_RS07960 at 1787.384 kb on - strand, within LRK53_RS07960 at 1787.384 kb on - strand, within LRK53_RS07960 at 1787.384 kb on - strand, within LRK53_RS07960 at 1787.384 kb on - strand, within LRK53_RS07960 at 1787.428 kb on + strand, within LRK53_RS07960 at 1787.428 kb on + strand, within LRK53_RS07960 at 1787.428 kb on + strand, within LRK53_RS07960 at 1787.428 kb on + strand, within LRK53_RS07960 at 1787.428 kb on + strand, within LRK53_RS07960 at 1787.428 kb on + strand, within LRK53_RS07960 at 1787.428 kb on + strand, within LRK53_RS07960 at 1787.428 kb on + strand, within LRK53_RS07960 at 1787.428 kb on + strand, within LRK53_RS07960 at 1787.429 kb on - strand, within LRK53_RS07960 at 1787.429 kb on - strand, within LRK53_RS07960 at 1787.429 kb on - strand, within LRK53_RS07960 at 1787.491 kb on + strand, within LRK53_RS07960 at 1787.492 kb on - strand, within LRK53_RS07960 at 1787.534 kb on - strand, within LRK53_RS07960 at 1787.584 kb on + strand, within LRK53_RS07960 at 1787.584 kb on + strand, within LRK53_RS07960 at 1787.585 kb on - strand, within LRK53_RS07960 at 1787.585 kb on - strand, within LRK53_RS07960 at 1787.642 kb on - strand, within LRK53_RS07960 at 1787.642 kb on - strand, within LRK53_RS07960 at 1787.642 kb on - strand, within LRK53_RS07960 at 1787.659 kb on + strand, within LRK53_RS07960 at 1787.659 kb on + strand, within LRK53_RS07960 at 1787.659 kb on + strand, within LRK53_RS07960 at 1787.659 kb on + strand, within LRK53_RS07960 at 1787.659 kb on + strand, within LRK53_RS07960 at 1787.660 kb on - strand, within LRK53_RS07960 at 1787.660 kb on - strand, within LRK53_RS07960 at 1787.660 kb on - strand, within LRK53_RS07960 at 1787.660 kb on - strand, within LRK53_RS07960 at 1787.660 kb on - strand, within LRK53_RS07960 at 1787.706 kb on + strand, within LRK53_RS07960 at 1787.706 kb on + strand, within LRK53_RS07960 at 1787.706 kb on + strand, within LRK53_RS07960 at 1787.706 kb on + strand, within LRK53_RS07960 at 1787.707 kb on - strand, within LRK53_RS07960 at 1787.707 kb on - strand, within LRK53_RS07960 at 1787.707 kb on - strand, within LRK53_RS07960 at 1787.745 kb on - strand, within LRK53_RS07960 at 1787.745 kb on - strand, within LRK53_RS07960 at 1787.810 kb on + strand, within LRK53_RS07960 at 1787.810 kb on + strand, within LRK53_RS07960 at 1787.811 kb on - strand, within LRK53_RS07960 at 1787.811 kb on - strand, within LRK53_RS07960 at 1787.811 kb on - strand, within LRK53_RS07960 at 1787.945 kb on + strand, within LRK53_RS07960 at 1787.945 kb on + strand, within LRK53_RS07960 at 1787.945 kb on + strand, within LRK53_RS07960 at 1787.945 kb on + strand, within LRK53_RS07960 at 1787.945 kb on + strand, within LRK53_RS07960 at 1787.945 kb on + strand, within LRK53_RS07960 at 1787.945 kb on + strand, within LRK53_RS07960 at 1787.945 kb on + strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1787.946 kb on - strand, within LRK53_RS07960 at 1788.031 kb on + strand, within LRK53_RS07960 at 1788.032 kb on - strand, within LRK53_RS07960 at 1788.032 kb on - strand, within LRK53_RS07960
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 1,785,116 + +0.3 1,785,117 - -2.5 1,785,126 + -1.3 1,785,126 + -0.4 1,785,126 + -0.1 1,785,126 + -1.9 1,785,127 - -0.4 1,785,127 - +0.9 1,785,140 + +0.9 1,785,141 - -0.3 1,785,141 - -0.3 1,785,141 - +0.0 1,785,141 - -1.4 1,785,146 + +0.9 1,785,146 + -0.7 1,785,146 + -1.1 1,785,146 + -0.1 1,785,146 + -0.8 1,785,146 + -0.3 1,785,146 + +0.9 1,785,146 + -0.5 1,785,146 + +1.9 1,785,146 + -0.4 1,785,147 - -1.1 1,785,951 + -2.5 1,785,951 + -0.9 1,785,951 + +1.9 1,785,952 - -1.1 1,785,952 - +0.2 1,786,044 + -0.4 1,786,044 + +0.9 1,786,044 + -1.9 1,786,044 + -0.1 1,786,044 + -1.3 1,786,045 - -0.1 1,786,070 + -1.9 1,786,070 + -0.1 1,786,070 + -1.7 1,786,071 - -1.1 1,786,071 - -0.2 1,786,071 - +0.6 1,786,071 - -0.7 1,786,071 - +0.5 1,786,071 - -0.1 1,786,071 - +0.9 1,786,115 - -1.9 1,786,115 - -1.7 1,786,115 - -0.1 1,786,115 - +0.9 1,786,115 - -0.5 1,786,115 - +1.9 1,786,115 - -1.1 1,786,419 + LRK53_RS07955 0.34 +2.5 1,786,419 + LRK53_RS07955 0.34 +0.3 1,786,420 - LRK53_RS07955 0.34 +2.9 1,786,420 - LRK53_RS07955 0.34 -1.8 1,786,420 - LRK53_RS07955 0.34 -0.1 1,786,420 - LRK53_RS07955 0.34 -1.1 1,786,473 + LRK53_RS07955 0.40 -0.5 1,786,474 - LRK53_RS07955 0.40 +0.3 1,786,474 - LRK53_RS07955 0.40 +0.9 1,786,474 - LRK53_RS07955 0.40 +1.3 1,786,474 - LRK53_RS07955 0.40 +1.9 1,786,474 - LRK53_RS07955 0.40 -0.1 1,786,474 - LRK53_RS07955 0.40 +0.6 1,787,068 + +0.0 1,787,068 + +0.2 1,787,068 + +0.9 1,787,069 - -0.1 1,787,069 - -2.2 1,787,069 - -0.1 1,787,069 - +0.4 1,787,069 - -0.5 1,787,201 + +0.9 1,787,201 + +0.3 1,787,201 + -1.1 1,787,202 - -0.1 1,787,202 - +0.5 1,787,202 - -1.3 1,787,202 - -0.1 1,787,202 - +0.9 1,787,202 - +0.3 1,787,254 + +0.5 1,787,254 + +0.5 1,787,254 + -1.7 1,787,254 + -1.7 1,787,254 + +1.2 1,787,255 - +1.9 1,787,255 - -0.1 1,787,255 - -1.7 1,787,255 - +0.9 1,787,383 + LRK53_RS07960 0.17 -2.3 1,787,383 + LRK53_RS07960 0.17 -1.4 1,787,383 + LRK53_RS07960 0.17 -1.0 1,787,383 + LRK53_RS07960 0.17 -0.1 1,787,383 + LRK53_RS07960 0.17 +0.2 1,787,383 + LRK53_RS07960 0.17 +0.9 1,787,383 + LRK53_RS07960 0.17 -1.2 1,787,383 + LRK53_RS07960 0.17 -0.4 1,787,383 + LRK53_RS07960 0.17 -0.1 1,787,383 + LRK53_RS07960 0.17 +0.3 1,787,383 + LRK53_RS07960 0.17 -0.9 1,787,384 - LRK53_RS07960 0.17 -0.8 1,787,384 - LRK53_RS07960 0.17 +0.2 1,787,384 - LRK53_RS07960 0.17 -0.7 1,787,384 - LRK53_RS07960 0.17 -1.4 1,787,384 - LRK53_RS07960 0.17 -1.2 1,787,384 - LRK53_RS07960 0.17 -0.7 1,787,384 - LRK53_RS07960 0.17 -0.3 1,787,384 - LRK53_RS07960 0.17 -0.7 1,787,384 - LRK53_RS07960 0.17 +0.5 1,787,384 - LRK53_RS07960 0.17 -0.7 1,787,384 - LRK53_RS07960 0.17 -1.3 1,787,428 + LRK53_RS07960 0.20 -0.5 1,787,428 + LRK53_RS07960 0.20 +1.9 1,787,428 + LRK53_RS07960 0.20 -0.6 1,787,428 + LRK53_RS07960 0.20 +0.9 1,787,428 + LRK53_RS07960 0.20 -0.4 1,787,428 + LRK53_RS07960 0.20 +1.5 1,787,428 + LRK53_RS07960 0.20 +0.2 1,787,428 + LRK53_RS07960 0.20 -0.1 1,787,428 + LRK53_RS07960 0.20 +0.9 1,787,429 - LRK53_RS07960 0.20 +0.9 1,787,429 - LRK53_RS07960 0.20 -1.5 1,787,429 - LRK53_RS07960 0.20 -0.1 1,787,491 + LRK53_RS07960 0.24 -0.1 1,787,492 - LRK53_RS07960 0.24 -0.2 1,787,534 - LRK53_RS07960 0.26 -0.1 1,787,584 + LRK53_RS07960 0.29 -5.0 1,787,584 + LRK53_RS07960 0.29 -1.9 1,787,585 - LRK53_RS07960 0.29 -0.1 1,787,585 - LRK53_RS07960 0.29 -1.4 1,787,642 - LRK53_RS07960 0.33 -0.1 1,787,642 - LRK53_RS07960 0.33 -0.4 1,787,642 - LRK53_RS07960 0.33 -1.7 1,787,659 + LRK53_RS07960 0.34 +0.5 1,787,659 + LRK53_RS07960 0.34 -1.4 1,787,659 + LRK53_RS07960 0.34 -0.7 1,787,659 + LRK53_RS07960 0.34 +1.2 1,787,659 + LRK53_RS07960 0.34 -0.1 1,787,660 - LRK53_RS07960 0.34 -0.3 1,787,660 - LRK53_RS07960 0.34 +0.9 1,787,660 - LRK53_RS07960 0.34 -1.7 1,787,660 - LRK53_RS07960 0.34 +1.9 1,787,660 - LRK53_RS07960 0.34 -1.9 1,787,706 + LRK53_RS07960 0.37 -0.9 1,787,706 + LRK53_RS07960 0.37 -0.4 1,787,706 + LRK53_RS07960 0.37 +2.9 1,787,706 + LRK53_RS07960 0.37 +0.9 1,787,707 - LRK53_RS07960 0.37 +1.2 1,787,707 - LRK53_RS07960 0.37 +0.1 1,787,707 - LRK53_RS07960 0.37 -0.8 1,787,745 - LRK53_RS07960 0.39 -0.7 1,787,745 - LRK53_RS07960 0.39 -1.3 1,787,810 + LRK53_RS07960 0.43 -0.4 1,787,810 + LRK53_RS07960 0.43 +0.9 1,787,811 - LRK53_RS07960 0.43 +1.9 1,787,811 - LRK53_RS07960 0.43 -0.7 1,787,811 - LRK53_RS07960 0.43 -0.7 1,787,945 + LRK53_RS07960 0.51 -0.5 1,787,945 + LRK53_RS07960 0.51 -0.0 1,787,945 + LRK53_RS07960 0.51 +0.1 1,787,945 + LRK53_RS07960 0.51 -1.2 1,787,945 + LRK53_RS07960 0.51 -0.1 1,787,945 + LRK53_RS07960 0.51 -1.1 1,787,945 + LRK53_RS07960 0.51 +0.9 1,787,945 + LRK53_RS07960 0.51 +0.2 1,787,946 - LRK53_RS07960 0.51 +0.3 1,787,946 - LRK53_RS07960 0.51 -0.8 1,787,946 - LRK53_RS07960 0.51 +0.3 1,787,946 - LRK53_RS07960 0.51 -1.8 1,787,946 - LRK53_RS07960 0.51 -0.1 1,787,946 - LRK53_RS07960 0.51 -1.4 1,787,946 - LRK53_RS07960 0.51 -0.4 1,787,946 - LRK53_RS07960 0.51 -0.5 1,787,946 - LRK53_RS07960 0.51 +0.1 1,787,946 - LRK53_RS07960 0.51 -0.3 1,787,946 - LRK53_RS07960 0.51 +0.2 1,787,946 - LRK53_RS07960 0.51 -0.7 1,787,946 - LRK53_RS07960 0.51 +0.9 1,787,946 - LRK53_RS07960 0.51 -1.4 1,787,946 - LRK53_RS07960 0.51 -0.1 1,787,946 - LRK53_RS07960 0.51 +0.7 1,787,946 - LRK53_RS07960 0.51 +0.2 1,787,946 - LRK53_RS07960 0.51 -2.7 1,787,946 - LRK53_RS07960 0.51 +0.3 1,788,031 + LRK53_RS07960 0.56 -1.3 1,788,032 - LRK53_RS07960 0.56 -0.7 1,788,032 - LRK53_RS07960 0.56 -1.0
Or see this region's nucleotide sequence