Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04805 and LRK53_RS04810 are separated by 200 nucleotides LRK53_RS04810 and LRK53_RS04815 are separated by 68 nucleotides LRK53_RS04815 and LRK53_RS04820 are separated by 339 nucleotides LRK53_RS04820 and LRK53_RS04825 are separated by 295 nucleotides
LRK53_RS04805: LRK53_RS04805 - hypothetical protein, at 1,120,400 to 1,121,494
_RS04805
LRK53_RS04810: LRK53_RS04810 - hypothetical protein, at 1,121,695 to 1,122,117
_RS04810
LRK53_RS04815: LRK53_RS04815 - IS5 family transposase, at 1,122,186 to 1,123,254
_RS04815
LRK53_RS04820: LRK53_RS04820 - c-type cytochrome, at 1,123,594 to 1,123,926
_RS04820
LRK53_RS04825: LRK53_RS04825 - IS1595 family transposase, at 1,124,222 to 1,125,166
_RS04825
Position (kb)
1122
1123
1124 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1121.219 kb on - strand, within LRK53_RS04805 at 1121.270 kb on - strand, within LRK53_RS04805 at 1121.385 kb on - strand at 1121.411 kb on - strand at 1121.411 kb on - strand at 1121.479 kb on + strand at 1121.509 kb on + strand at 1121.509 kb on + strand at 1121.509 kb on + strand at 1121.510 kb on - strand at 1121.510 kb on - strand at 1121.510 kb on - strand at 1121.510 kb on - strand at 1121.511 kb on + strand at 1121.555 kb on - strand at 1121.555 kb on - strand at 1121.578 kb on + strand at 1121.583 kb on - strand at 1121.617 kb on + strand at 1121.665 kb on + strand at 1121.678 kb on + strand at 1121.678 kb on + strand at 1121.679 kb on - strand at 1121.679 kb on - strand at 1121.679 kb on - strand at 1121.680 kb on + strand at 1121.680 kb on + strand at 1121.680 kb on + strand at 1121.680 kb on + strand at 1121.681 kb on - strand at 1121.694 kb on + strand at 1121.737 kb on - strand at 1121.737 kb on - strand at 1121.737 kb on - strand at 1121.760 kb on + strand, within LRK53_RS04810 at 1121.761 kb on - strand, within LRK53_RS04810 at 1121.761 kb on - strand, within LRK53_RS04810 at 1121.763 kb on + strand, within LRK53_RS04810 at 1121.764 kb on - strand, within LRK53_RS04810 at 1121.784 kb on + strand, within LRK53_RS04810 at 1121.786 kb on + strand, within LRK53_RS04810 at 1121.786 kb on - strand, within LRK53_RS04810 at 1121.868 kb on + strand, within LRK53_RS04810 at 1121.868 kb on + strand, within LRK53_RS04810 at 1121.872 kb on + strand, within LRK53_RS04810 at 1121.872 kb on + strand, within LRK53_RS04810 at 1121.872 kb on + strand, within LRK53_RS04810 at 1121.899 kb on + strand, within LRK53_RS04810 at 1121.899 kb on + strand, within LRK53_RS04810 at 1121.899 kb on + strand, within LRK53_RS04810 at 1121.900 kb on - strand, within LRK53_RS04810 at 1121.900 kb on - strand, within LRK53_RS04810 at 1121.900 kb on - strand, within LRK53_RS04810 at 1121.925 kb on + strand, within LRK53_RS04810 at 1121.926 kb on - strand, within LRK53_RS04810 at 1121.955 kb on + strand, within LRK53_RS04810 at 1121.956 kb on - strand, within LRK53_RS04810 at 1121.978 kb on + strand, within LRK53_RS04810 at 1121.978 kb on + strand, within LRK53_RS04810 at 1122.018 kb on + strand, within LRK53_RS04810 at 1122.019 kb on - strand, within LRK53_RS04810 at 1122.021 kb on + strand, within LRK53_RS04810 at 1122.022 kb on - strand, within LRK53_RS04810 at 1122.022 kb on - strand, within LRK53_RS04810 at 1122.084 kb on + strand at 1122.084 kb on + strand at 1122.085 kb on - strand at 1122.116 kb on - strand at 1122.116 kb on - strand at 1122.116 kb on - strand at 1122.116 kb on - strand at 1122.116 kb on - strand at 1122.116 kb on - strand at 1122.116 kb on - strand at 1122.116 kb on - strand at 1122.116 kb on - strand at 1122.116 kb on - strand at 1122.116 kb on - strand at 1122.168 kb on + strand at 1122.168 kb on + strand at 1122.433 kb on - strand, within LRK53_RS04815 at 1122.433 kb on - strand, within LRK53_RS04815 at 1122.433 kb on - strand, within LRK53_RS04815 at 1122.433 kb on - strand, within LRK53_RS04815 at 1122.433 kb on - strand, within LRK53_RS04815 at 1122.433 kb on - strand, within LRK53_RS04815 at 1122.433 kb on - strand, within LRK53_RS04815 at 1122.433 kb on - strand, within LRK53_RS04815 at 1123.371 kb on + strand at 1123.378 kb on + strand at 1123.379 kb on - strand at 1123.380 kb on + strand at 1123.380 kb on + strand at 1123.380 kb on + strand at 1123.380 kb on + strand at 1123.380 kb on + strand at 1123.381 kb on - strand at 1123.381 kb on - strand at 1123.381 kb on - strand at 1123.395 kb on + strand at 1123.396 kb on - strand at 1123.407 kb on + strand at 1123.444 kb on - strand at 1123.445 kb on + strand at 1123.445 kb on + strand at 1123.446 kb on - strand at 1123.446 kb on - strand at 1123.448 kb on - strand at 1123.479 kb on + strand at 1123.479 kb on + strand at 1123.532 kb on - strand at 1123.607 kb on - strand at 1123.610 kb on + strand at 1123.610 kb on + strand at 1123.610 kb on + strand at 1123.611 kb on - strand at 1123.611 kb on - strand at 1123.611 kb on - strand at 1123.615 kb on - strand at 1123.676 kb on + strand, within LRK53_RS04820 at 1123.676 kb on + strand, within LRK53_RS04820 at 1123.676 kb on + strand, within LRK53_RS04820 at 1123.677 kb on - strand, within LRK53_RS04820 at 1123.678 kb on + strand, within LRK53_RS04820 at 1123.678 kb on + strand, within LRK53_RS04820 at 1123.678 kb on + strand, within LRK53_RS04820 at 1123.679 kb on - strand, within LRK53_RS04820 at 1123.679 kb on - strand, within LRK53_RS04820 at 1123.679 kb on - strand, within LRK53_RS04820 at 1123.748 kb on + strand, within LRK53_RS04820 at 1123.748 kb on + strand, within LRK53_RS04820 at 1123.749 kb on - strand, within LRK53_RS04820 at 1123.749 kb on - strand, within LRK53_RS04820 at 1123.749 kb on - strand, within LRK53_RS04820 at 1123.749 kb on - strand, within LRK53_RS04820 at 1123.752 kb on + strand, within LRK53_RS04820 at 1123.890 kb on - strand, within LRK53_RS04820 at 1123.890 kb on - strand, within LRK53_RS04820 at 1123.890 kb on - strand, within LRK53_RS04820 at 1123.890 kb on - strand, within LRK53_RS04820 at 1123.890 kb on - strand, within LRK53_RS04820 at 1123.914 kb on + strand at 1123.925 kb on - strand at 1123.966 kb on + strand at 1123.974 kb on + strand at 1123.979 kb on - strand at 1124.016 kb on + strand at 1124.017 kb on - strand at 1124.017 kb on - strand at 1124.068 kb on + strand at 1124.069 kb on - strand at 1124.069 kb on - strand at 1124.096 kb on - strand at 1124.118 kb on + strand at 1124.118 kb on + strand at 1124.136 kb on + strand at 1124.191 kb on - strand at 1124.213 kb on + strand at 1124.213 kb on + strand at 1124.213 kb on + strand at 1124.213 kb on + strand at 1124.213 kb on + strand at 1124.213 kb on + strand at 1124.213 kb on + strand at 1124.213 kb on + strand at 1124.214 kb on - strand at 1124.214 kb on - strand at 1124.214 kb on - strand at 1124.214 kb on - strand at 1124.216 kb on + strand at 1124.216 kb on + strand at 1124.216 kb on + strand at 1124.216 kb on + strand at 1124.216 kb on + strand at 1124.216 kb on + strand at 1124.216 kb on + strand at 1124.216 kb on + strand at 1124.217 kb on - strand at 1124.217 kb on - strand at 1124.217 kb on - strand at 1124.217 kb on - strand at 1124.217 kb on - strand at 1124.217 kb on - strand at 1124.217 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 1,121,219 - LRK53_RS04805 0.75 -1.4 1,121,270 - LRK53_RS04805 0.79 -1.1 1,121,385 - +0.2 1,121,411 - -0.6 1,121,411 - -0.5 1,121,479 + -0.3 1,121,509 + -1.6 1,121,509 + +0.9 1,121,509 + -0.4 1,121,510 - -0.1 1,121,510 - -0.3 1,121,510 - +0.3 1,121,510 - +0.3 1,121,511 + -0.3 1,121,555 - -0.6 1,121,555 - -0.8 1,121,578 + -0.1 1,121,583 - -1.7 1,121,617 + -0.5 1,121,665 + -1.3 1,121,678 + -1.8 1,121,678 + -0.6 1,121,679 - +0.7 1,121,679 - -1.1 1,121,679 - -1.4 1,121,680 + +0.9 1,121,680 + +0.9 1,121,680 + -1.1 1,121,680 + -1.1 1,121,681 - +1.5 1,121,694 + -0.6 1,121,737 - +0.4 1,121,737 - -1.8 1,121,737 - +0.0 1,121,760 + LRK53_RS04810 0.15 -0.1 1,121,761 - LRK53_RS04810 0.16 -0.5 1,121,761 - LRK53_RS04810 0.16 -0.6 1,121,763 + LRK53_RS04810 0.16 +0.4 1,121,764 - LRK53_RS04810 0.16 +0.4 1,121,784 + LRK53_RS04810 0.21 +0.9 1,121,786 + LRK53_RS04810 0.22 +0.5 1,121,786 - LRK53_RS04810 0.22 -0.6 1,121,868 + LRK53_RS04810 0.41 -0.6 1,121,868 + LRK53_RS04810 0.41 -0.3 1,121,872 + LRK53_RS04810 0.42 -0.1 1,121,872 + LRK53_RS04810 0.42 -0.7 1,121,872 + LRK53_RS04810 0.42 -1.4 1,121,899 + LRK53_RS04810 0.48 +1.9 1,121,899 + LRK53_RS04810 0.48 -0.1 1,121,899 + LRK53_RS04810 0.48 -0.1 1,121,900 - LRK53_RS04810 0.48 +0.2 1,121,900 - LRK53_RS04810 0.48 -0.1 1,121,900 - LRK53_RS04810 0.48 -1.1 1,121,925 + LRK53_RS04810 0.54 -0.6 1,121,926 - LRK53_RS04810 0.55 -0.1 1,121,955 + LRK53_RS04810 0.61 -0.9 1,121,956 - LRK53_RS04810 0.62 +0.9 1,121,978 + LRK53_RS04810 0.67 -1.1 1,121,978 + LRK53_RS04810 0.67 -0.9 1,122,018 + LRK53_RS04810 0.76 -0.7 1,122,019 - LRK53_RS04810 0.77 -1.1 1,122,021 + LRK53_RS04810 0.77 -1.1 1,122,022 - LRK53_RS04810 0.77 -0.6 1,122,022 - LRK53_RS04810 0.77 -0.4 1,122,084 + -1.2 1,122,084 + -0.9 1,122,085 - -0.3 1,122,116 - -0.8 1,122,116 - +2.2 1,122,116 - +0.9 1,122,116 - -0.6 1,122,116 - -1.0 1,122,116 - +0.4 1,122,116 - +0.9 1,122,116 - -1.3 1,122,116 - -1.9 1,122,116 - +0.9 1,122,116 - -0.9 1,122,168 + +0.6 1,122,168 + +0.5 1,122,433 - LRK53_RS04815 0.23 -0.6 1,122,433 - LRK53_RS04815 0.23 -0.1 1,122,433 - LRK53_RS04815 0.23 +0.9 1,122,433 - LRK53_RS04815 0.23 +0.3 1,122,433 - LRK53_RS04815 0.23 +0.9 1,122,433 - LRK53_RS04815 0.23 -1.4 1,122,433 - LRK53_RS04815 0.23 -0.9 1,122,433 - LRK53_RS04815 0.23 -0.6 1,123,371 + +0.1 1,123,378 + -0.1 1,123,379 - +0.2 1,123,380 + -0.9 1,123,380 + -1.9 1,123,380 + -1.9 1,123,380 + +0.4 1,123,380 + +0.4 1,123,381 - -0.2 1,123,381 - -0.1 1,123,381 - +1.5 1,123,395 + -0.6 1,123,396 - -0.2 1,123,407 + +0.9 1,123,444 - -0.8 1,123,445 + -1.6 1,123,445 + +0.1 1,123,446 - -0.6 1,123,446 - -0.5 1,123,448 - -2.0 1,123,479 + -0.6 1,123,479 + -0.6 1,123,532 - +0.4 1,123,607 - -1.4 1,123,610 + -0.6 1,123,610 + -0.1 1,123,610 + -1.0 1,123,611 - -0.6 1,123,611 - +0.9 1,123,611 - -0.6 1,123,615 - -0.8 1,123,676 + LRK53_RS04820 0.25 -1.3 1,123,676 + LRK53_RS04820 0.25 +0.5 1,123,676 + LRK53_RS04820 0.25 -0.6 1,123,677 - LRK53_RS04820 0.25 +0.9 1,123,678 + LRK53_RS04820 0.25 +0.9 1,123,678 + LRK53_RS04820 0.25 -2.0 1,123,678 + LRK53_RS04820 0.25 -1.2 1,123,679 - LRK53_RS04820 0.26 -2.4 1,123,679 - LRK53_RS04820 0.26 -0.1 1,123,679 - LRK53_RS04820 0.26 -0.4 1,123,748 + LRK53_RS04820 0.46 -0.9 1,123,748 + LRK53_RS04820 0.46 +0.1 1,123,749 - LRK53_RS04820 0.47 -0.9 1,123,749 - LRK53_RS04820 0.47 -0.1 1,123,749 - LRK53_RS04820 0.47 -0.7 1,123,749 - LRK53_RS04820 0.47 -1.1 1,123,752 + LRK53_RS04820 0.47 +0.3 1,123,890 - LRK53_RS04820 0.89 +0.9 1,123,890 - LRK53_RS04820 0.89 -0.1 1,123,890 - LRK53_RS04820 0.89 +0.9 1,123,890 - LRK53_RS04820 0.89 -1.7 1,123,890 - LRK53_RS04820 0.89 +0.9 1,123,914 + +0.1 1,123,925 - -0.7 1,123,966 + +0.4 1,123,974 + -0.1 1,123,979 - -0.7 1,124,016 + +0.9 1,124,017 - +1.5 1,124,017 - -0.1 1,124,068 + +1.5 1,124,069 - +0.3 1,124,069 - -0.6 1,124,096 - +0.7 1,124,118 + -1.4 1,124,118 + -0.6 1,124,136 + -1.1 1,124,191 - -0.6 1,124,213 + +1.9 1,124,213 + -1.3 1,124,213 + -0.9 1,124,213 + -0.3 1,124,213 + +0.5 1,124,213 + -1.1 1,124,213 + -1.1 1,124,213 + -1.5 1,124,214 - +1.3 1,124,214 - +0.9 1,124,214 - -0.5 1,124,214 - -1.1 1,124,216 + -0.5 1,124,216 + -1.2 1,124,216 + -0.5 1,124,216 + -0.8 1,124,216 + -1.4 1,124,216 + -1.5 1,124,216 + -0.9 1,124,216 + -0.1 1,124,217 - -0.5 1,124,217 - -0.8 1,124,217 - -1.0 1,124,217 - +0.9 1,124,217 - +1.9 1,124,217 - +0.0 1,124,217 - +0.9
Or see this region's nucleotide sequence