Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04125 and LRK53_RS04130 are separated by 136 nucleotides LRK53_RS04130 and LRK53_RS04135 are separated by 49 nucleotides LRK53_RS04135 and LRK53_RS04140 are separated by 509 nucleotides
LRK53_RS04125: LRK53_RS04125 - ferritin-like domain-containing protein, at 966,731 to 967,552
_RS04125
LRK53_RS04130: LRK53_RS04130 - universal stress protein, at 967,689 to 968,105
_RS04130
LRK53_RS04135: LRK53_RS04135 - glycine zipper 2TM domain-containing protein, at 968,155 to 968,325
_RS04135
LRK53_RS04140: LRK53_RS04140 - hypothetical protein, at 968,835 to 969,293
_RS04140
Position (kb) 967
968
969 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 967.047 kb on + strand, within LRK53_RS04125 at 967.056 kb on + strand, within LRK53_RS04125 at 967.056 kb on + strand, within LRK53_RS04125 at 967.062 kb on + strand, within LRK53_RS04125 at 967.062 kb on + strand, within LRK53_RS04125 at 967.062 kb on + strand, within LRK53_RS04125 at 967.062 kb on + strand, within LRK53_RS04125 at 967.062 kb on + strand, within LRK53_RS04125 at 967.063 kb on - strand, within LRK53_RS04125 at 967.063 kb on - strand, within LRK53_RS04125 at 967.063 kb on - strand, within LRK53_RS04125 at 967.063 kb on - strand, within LRK53_RS04125 at 967.063 kb on - strand, within LRK53_RS04125 at 967.242 kb on + strand, within LRK53_RS04125 at 967.242 kb on + strand, within LRK53_RS04125 at 967.242 kb on + strand, within LRK53_RS04125 at 967.243 kb on - strand, within LRK53_RS04125 at 967.305 kb on + strand, within LRK53_RS04125 at 967.305 kb on + strand, within LRK53_RS04125 at 967.305 kb on + strand, within LRK53_RS04125 at 967.306 kb on - strand, within LRK53_RS04125 at 967.306 kb on - strand, within LRK53_RS04125 at 967.306 kb on - strand, within LRK53_RS04125 at 967.306 kb on - strand, within LRK53_RS04125 at 967.306 kb on - strand, within LRK53_RS04125 at 967.306 kb on - strand, within LRK53_RS04125 at 967.306 kb on - strand, within LRK53_RS04125 at 967.306 kb on - strand, within LRK53_RS04125 at 967.413 kb on + strand, within LRK53_RS04125 at 967.413 kb on + strand, within LRK53_RS04125 at 967.413 kb on + strand, within LRK53_RS04125 at 967.413 kb on + strand, within LRK53_RS04125 at 967.414 kb on - strand, within LRK53_RS04125 at 967.568 kb on - strand at 967.638 kb on + strand at 967.638 kb on + strand at 967.638 kb on + strand at 967.638 kb on + strand at 967.639 kb on - strand at 967.639 kb on - strand at 967.639 kb on - strand at 967.639 kb on - strand at 967.639 kb on - strand at 967.639 kb on - strand at 967.639 kb on - strand at 967.782 kb on - strand, within LRK53_RS04130 at 967.782 kb on - strand, within LRK53_RS04130 at 968.001 kb on + strand, within LRK53_RS04130 at 968.001 kb on + strand, within LRK53_RS04130 at 968.001 kb on + strand, within LRK53_RS04130 at 968.001 kb on + strand, within LRK53_RS04130 at 968.001 kb on + strand, within LRK53_RS04130 at 968.001 kb on + strand, within LRK53_RS04130 at 968.001 kb on + strand, within LRK53_RS04130 at 968.001 kb on + strand, within LRK53_RS04130 at 968.002 kb on - strand, within LRK53_RS04130 at 968.002 kb on - strand, within LRK53_RS04130 at 968.002 kb on - strand, within LRK53_RS04130 at 968.081 kb on - strand at 968.081 kb on - strand at 968.081 kb on - strand at 968.081 kb on - strand at 968.095 kb on + strand at 968.095 kb on + strand at 968.095 kb on + strand at 968.095 kb on + strand at 968.095 kb on + strand at 968.096 kb on - strand at 968.096 kb on - strand at 968.096 kb on - strand at 968.096 kb on - strand at 968.096 kb on - strand at 968.096 kb on - strand at 968.096 kb on - strand at 968.096 kb on - strand at 968.096 kb on - strand at 968.188 kb on - strand, within LRK53_RS04135 at 968.234 kb on + strand, within LRK53_RS04135 at 968.443 kb on - strand at 968.552 kb on + strand at 968.552 kb on + strand at 968.553 kb on - strand at 968.553 kb on - strand at 968.619 kb on + strand at 968.619 kb on + strand at 968.620 kb on - strand at 968.620 kb on - strand at 968.628 kb on + strand at 968.628 kb on + strand at 968.640 kb on - strand at 968.657 kb on + strand at 968.657 kb on + strand at 968.657 kb on + strand at 968.657 kb on + strand at 968.657 kb on + strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.787 kb on + strand at 968.787 kb on + strand at 968.787 kb on + strand at 968.787 kb on + strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.846 kb on + strand at 968.846 kb on + strand at 968.846 kb on + strand at 968.846 kb on + strand at 968.846 kb on + strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.979 kb on + strand, within LRK53_RS04140 at 968.979 kb on + strand, within LRK53_RS04140 at 968.979 kb on + strand, within LRK53_RS04140 at 968.980 kb on - strand, within LRK53_RS04140 at 968.980 kb on - strand, within LRK53_RS04140 at 968.980 kb on - strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.079 kb on - strand, within LRK53_RS04140 at 969.079 kb on - strand, within LRK53_RS04140 at 969.079 kb on - strand, within LRK53_RS04140 at 969.079 kb on - strand, within LRK53_RS04140
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 967,047 + LRK53_RS04125 0.38 +1.9 967,056 + LRK53_RS04125 0.40 -0.6 967,056 + LRK53_RS04125 0.40 -1.1 967,062 + LRK53_RS04125 0.40 -1.2 967,062 + LRK53_RS04125 0.40 -0.6 967,062 + LRK53_RS04125 0.40 -0.1 967,062 + LRK53_RS04125 0.40 -1.4 967,062 + LRK53_RS04125 0.40 -0.9 967,063 - LRK53_RS04125 0.40 -0.1 967,063 - LRK53_RS04125 0.40 -0.6 967,063 - LRK53_RS04125 0.40 +1.5 967,063 - LRK53_RS04125 0.40 +0.2 967,063 - LRK53_RS04125 0.40 -0.1 967,242 + LRK53_RS04125 0.62 -1.4 967,242 + LRK53_RS04125 0.62 +1.9 967,242 + LRK53_RS04125 0.62 +0.4 967,243 - LRK53_RS04125 0.62 +0.9 967,305 + LRK53_RS04125 0.70 -2.2 967,305 + LRK53_RS04125 0.70 -0.4 967,305 + LRK53_RS04125 0.70 +0.1 967,306 - LRK53_RS04125 0.70 -1.2 967,306 - LRK53_RS04125 0.70 -1.1 967,306 - LRK53_RS04125 0.70 +0.0 967,306 - LRK53_RS04125 0.70 +0.4 967,306 - LRK53_RS04125 0.70 -0.1 967,306 - LRK53_RS04125 0.70 -1.1 967,306 - LRK53_RS04125 0.70 -1.1 967,306 - LRK53_RS04125 0.70 +0.5 967,413 + LRK53_RS04125 0.83 -0.5 967,413 + LRK53_RS04125 0.83 -0.1 967,413 + LRK53_RS04125 0.83 -0.6 967,413 + LRK53_RS04125 0.83 +0.4 967,414 - LRK53_RS04125 0.83 +0.4 967,568 - +0.9 967,638 + -1.2 967,638 + -1.8 967,638 + -1.4 967,638 + -0.7 967,639 - -2.4 967,639 - -1.1 967,639 - -0.6 967,639 - +0.5 967,639 - -1.5 967,639 - -0.3 967,639 - +0.6 967,782 - LRK53_RS04130 0.22 -0.7 967,782 - LRK53_RS04130 0.22 -0.3 968,001 + LRK53_RS04130 0.75 -0.1 968,001 + LRK53_RS04130 0.75 -2.1 968,001 + LRK53_RS04130 0.75 +0.1 968,001 + LRK53_RS04130 0.75 +0.9 968,001 + LRK53_RS04130 0.75 +0.9 968,001 + LRK53_RS04130 0.75 -1.2 968,001 + LRK53_RS04130 0.75 +0.9 968,001 + LRK53_RS04130 0.75 +0.7 968,002 - LRK53_RS04130 0.75 -0.9 968,002 - LRK53_RS04130 0.75 -0.6 968,002 - LRK53_RS04130 0.75 +0.4 968,081 - -1.1 968,081 - -2.6 968,081 - -0.6 968,081 - -0.1 968,095 + +0.9 968,095 + -0.1 968,095 + +0.9 968,095 + -0.1 968,095 + -0.5 968,096 - +0.2 968,096 - -2.2 968,096 - -0.1 968,096 - -0.5 968,096 - -0.2 968,096 - +0.9 968,096 - -0.9 968,096 - -0.4 968,096 - -0.6 968,188 - LRK53_RS04135 0.19 -1.4 968,234 + LRK53_RS04135 0.46 +1.5 968,443 - +1.8 968,552 + -0.6 968,552 + -1.1 968,553 - -1.4 968,553 - -1.2 968,619 + -0.1 968,619 + +1.9 968,620 - -0.1 968,620 - +0.4 968,628 + -0.6 968,628 + -0.1 968,640 - -0.4 968,657 + -0.1 968,657 + -0.8 968,657 + +0.5 968,657 + -0.3 968,657 + +0.9 968,658 - -0.1 968,658 - -1.8 968,658 - -0.1 968,658 - +0.9 968,658 - +1.9 968,658 - +0.9 968,658 - -0.9 968,658 - -0.6 968,787 + -0.3 968,787 + +0.1 968,787 + -0.4 968,787 + +1.9 968,788 - +0.5 968,788 - -0.4 968,788 - -0.8 968,788 - -0.6 968,788 - +0.4 968,788 - -0.6 968,788 - -0.6 968,788 - -0.6 968,801 + +1.9 968,801 + -0.3 968,801 + +0.9 968,801 + -1.6 968,801 + +0.4 968,801 + -0.3 968,801 + -0.6 968,801 + +2.4 968,801 + +0.5 968,801 + -2.5 968,801 + +0.9 968,801 + -0.6 968,801 + +0.1 968,801 + -0.1 968,802 - -0.4 968,802 - -2.1 968,802 - -1.1 968,802 - +0.2 968,802 - -1.1 968,802 - +1.5 968,802 - -0.1 968,802 - -1.1 968,802 - -1.1 968,802 - -1.4 968,802 - +0.9 968,802 - -0.1 968,802 - -2.2 968,802 - -1.3 968,802 - -0.1 968,802 - -0.2 968,802 - -0.6 968,846 + -0.1 968,846 + +0.9 968,846 + +0.5 968,846 + +0.9 968,846 + -0.1 968,847 - -0.4 968,847 - -1.1 968,847 - +0.2 968,847 - -0.9 968,847 - -1.6 968,847 - +0.4 968,847 - -1.4 968,847 - -1.5 968,952 + LRK53_RS04140 0.25 +0.2 968,952 + LRK53_RS04140 0.25 -0.1 968,952 + LRK53_RS04140 0.25 -1.0 968,952 + LRK53_RS04140 0.25 -1.0 968,952 + LRK53_RS04140 0.25 -1.4 968,952 + LRK53_RS04140 0.25 -1.2 968,952 + LRK53_RS04140 0.25 +0.7 968,952 + LRK53_RS04140 0.25 -0.6 968,952 + LRK53_RS04140 0.25 +0.6 968,952 + LRK53_RS04140 0.25 -0.1 968,952 + LRK53_RS04140 0.25 +0.2 968,952 + LRK53_RS04140 0.25 +1.9 968,952 + LRK53_RS04140 0.25 -0.1 968,952 + LRK53_RS04140 0.25 -2.2 968,952 + LRK53_RS04140 0.25 +2.5 968,953 - LRK53_RS04140 0.26 -1.4 968,953 - LRK53_RS04140 0.26 -1.4 968,953 - LRK53_RS04140 0.26 +0.1 968,953 - LRK53_RS04140 0.26 -0.5 968,953 - LRK53_RS04140 0.26 -0.1 968,953 - LRK53_RS04140 0.26 +0.9 968,953 - LRK53_RS04140 0.26 -1.6 968,953 - LRK53_RS04140 0.26 +1.5 968,953 - LRK53_RS04140 0.26 -1.1 968,953 - LRK53_RS04140 0.26 +0.6 968,979 + LRK53_RS04140 0.31 -2.6 968,979 + LRK53_RS04140 0.31 -0.6 968,979 + LRK53_RS04140 0.31 +1.5 968,980 - LRK53_RS04140 0.32 -0.2 968,980 - LRK53_RS04140 0.32 +0.5 968,980 - LRK53_RS04140 0.32 -0.6 969,078 + LRK53_RS04140 0.53 +1.4 969,078 + LRK53_RS04140 0.53 +0.9 969,078 + LRK53_RS04140 0.53 +0.9 969,078 + LRK53_RS04140 0.53 +0.9 969,078 + LRK53_RS04140 0.53 +0.9 969,078 + LRK53_RS04140 0.53 -3.6 969,078 + LRK53_RS04140 0.53 -1.4 969,078 + LRK53_RS04140 0.53 -0.1 969,079 - LRK53_RS04140 0.53 +0.9 969,079 - LRK53_RS04140 0.53 +0.9 969,079 - LRK53_RS04140 0.53 -1.6 969,079 - LRK53_RS04140 0.53 -1.1
Or see this region's nucleotide sequence