Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03845 and LRK53_RS03850 are separated by 68 nucleotides LRK53_RS03850 and LRK53_RS03855 overlap by 4 nucleotides LRK53_RS03855 and LRK53_RS03860 are separated by 210 nucleotides
LRK53_RS03845: LRK53_RS03845 - winged helix-turn-helix domain-containing protein, at 899,098 to 899,622
_RS03845
LRK53_RS03850: LRK53_RS03850 - HutD family protein, at 899,691 to 900,251
_RS03850
LRK53_RS03855: LRK53_RS03855 - ATP-dependent DNA helicase DinG, at 900,248 to 902,365
_RS03855
LRK53_RS03860: LRK53_RS03860 - EAL domain-containing protein, at 902,576 to 905,536
_RS03860
Position (kb) 900
901
902
903 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 899.656 kb on + strand at 899.660 kb on + strand at 899.743 kb on - strand at 899.886 kb on + strand, within LRK53_RS03850 at 899.886 kb on + strand, within LRK53_RS03850 at 899.962 kb on - strand, within LRK53_RS03850 at 899.962 kb on - strand, within LRK53_RS03850 at 899.962 kb on - strand, within LRK53_RS03850 at 899.962 kb on - strand, within LRK53_RS03850 at 899.962 kb on - strand, within LRK53_RS03850 at 899.999 kb on + strand, within LRK53_RS03850 at 899.999 kb on + strand, within LRK53_RS03850 at 899.999 kb on + strand, within LRK53_RS03850 at 900.000 kb on - strand, within LRK53_RS03850 at 900.000 kb on - strand, within LRK53_RS03850 at 900.000 kb on - strand, within LRK53_RS03850 at 900.183 kb on + strand, within LRK53_RS03850 at 900.183 kb on + strand, within LRK53_RS03850 at 900.184 kb on - strand, within LRK53_RS03850 at 900.184 kb on - strand, within LRK53_RS03850 at 900.387 kb on + strand at 900.387 kb on + strand at 900.387 kb on + strand at 900.388 kb on - strand at 900.388 kb on - strand at 900.388 kb on - strand at 900.388 kb on - strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.428 kb on + strand at 900.429 kb on - strand at 900.429 kb on - strand at 900.429 kb on - strand at 900.429 kb on - strand at 900.429 kb on - strand at 900.429 kb on - strand at 900.429 kb on - strand at 900.429 kb on - strand at 900.429 kb on - strand at 900.468 kb on + strand, within LRK53_RS03855 at 900.468 kb on + strand, within LRK53_RS03855 at 900.468 kb on + strand, within LRK53_RS03855 at 900.468 kb on + strand, within LRK53_RS03855 at 900.468 kb on + strand, within LRK53_RS03855 at 900.468 kb on + strand, within LRK53_RS03855 at 900.468 kb on + strand, within LRK53_RS03855 at 900.468 kb on + strand, within LRK53_RS03855 at 900.468 kb on + strand, within LRK53_RS03855 at 900.468 kb on + strand, within LRK53_RS03855 at 900.469 kb on - strand, within LRK53_RS03855 at 900.469 kb on - strand, within LRK53_RS03855 at 900.469 kb on - strand, within LRK53_RS03855 at 900.469 kb on - strand, within LRK53_RS03855 at 900.469 kb on - strand, within LRK53_RS03855 at 900.469 kb on - strand, within LRK53_RS03855 at 900.469 kb on - strand, within LRK53_RS03855 at 900.469 kb on - strand, within LRK53_RS03855 at 901.464 kb on + strand, within LRK53_RS03855 at 901.465 kb on - strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.584 kb on + strand, within LRK53_RS03855 at 901.585 kb on - strand, within LRK53_RS03855 at 901.585 kb on - strand, within LRK53_RS03855 at 901.585 kb on - strand, within LRK53_RS03855 at 901.585 kb on - strand, within LRK53_RS03855 at 901.585 kb on - strand, within LRK53_RS03855 at 901.585 kb on - strand, within LRK53_RS03855 at 901.585 kb on - strand, within LRK53_RS03855 at 901.585 kb on - strand, within LRK53_RS03855 at 901.585 kb on - strand, within LRK53_RS03855 at 902.016 kb on + strand, within LRK53_RS03855 at 902.016 kb on + strand, within LRK53_RS03855 at 902.017 kb on - strand, within LRK53_RS03855 at 902.017 kb on - strand, within LRK53_RS03855 at 902.073 kb on + strand, within LRK53_RS03855 at 902.073 kb on + strand, within LRK53_RS03855 at 902.073 kb on + strand, within LRK53_RS03855 at 902.073 kb on + strand, within LRK53_RS03855 at 902.073 kb on + strand, within LRK53_RS03855 at 902.074 kb on - strand, within LRK53_RS03855 at 902.181 kb on + strand at 902.181 kb on + strand at 902.181 kb on + strand at 902.181 kb on + strand at 902.182 kb on - strand at 902.182 kb on - strand at 902.182 kb on - strand at 902.182 kb on - strand at 902.182 kb on - strand at 902.182 kb on - strand at 902.325 kb on + strand at 902.325 kb on + strand at 902.326 kb on - strand at 902.454 kb on + strand at 902.460 kb on + strand at 902.461 kb on - strand at 903.152 kb on + strand, within LRK53_RS03860 at 903.152 kb on + strand, within LRK53_RS03860 at 903.152 kb on + strand, within LRK53_RS03860 at 903.152 kb on + strand, within LRK53_RS03860 at 903.152 kb on + strand, within LRK53_RS03860 at 903.152 kb on + strand, within LRK53_RS03860 at 903.152 kb on + strand, within LRK53_RS03860 at 903.152 kb on + strand, within LRK53_RS03860 at 903.152 kb on + strand, within LRK53_RS03860 at 903.152 kb on + strand, within LRK53_RS03860 at 903.152 kb on + strand, within LRK53_RS03860 at 903.152 kb on + strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.153 kb on - strand, within LRK53_RS03860 at 903.212 kb on + strand, within LRK53_RS03860 at 903.213 kb on - strand, within LRK53_RS03860 at 903.213 kb on - strand, within LRK53_RS03860 at 903.213 kb on - strand, within LRK53_RS03860 at 903.213 kb on - strand, within LRK53_RS03860 at 903.213 kb on - strand, within LRK53_RS03860 at 903.213 kb on - strand, within LRK53_RS03860 at 903.213 kb on - strand, within LRK53_RS03860 at 903.296 kb on + strand, within LRK53_RS03860 at 903.296 kb on + strand, within LRK53_RS03860 at 903.296 kb on + strand, within LRK53_RS03860 at 903.296 kb on + strand, within LRK53_RS03860 at 903.297 kb on - strand, within LRK53_RS03860 at 903.297 kb on - strand, within LRK53_RS03860 at 903.297 kb on - strand, within LRK53_RS03860 at 903.297 kb on - strand, within LRK53_RS03860 at 903.297 kb on - strand, within LRK53_RS03860 at 903.297 kb on - strand, within LRK53_RS03860 at 903.297 kb on - strand, within LRK53_RS03860 at 903.297 kb on - strand, within LRK53_RS03860
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 899,656 + -1.0 899,660 + +0.5 899,743 - -0.5 899,886 + LRK53_RS03850 0.35 -0.5 899,886 + LRK53_RS03850 0.35 -0.4 899,962 - LRK53_RS03850 0.48 -1.7 899,962 - LRK53_RS03850 0.48 -0.6 899,962 - LRK53_RS03850 0.48 -0.4 899,962 - LRK53_RS03850 0.48 +1.0 899,962 - LRK53_RS03850 0.48 -0.6 899,999 + LRK53_RS03850 0.55 -0.6 899,999 + LRK53_RS03850 0.55 +1.4 899,999 + LRK53_RS03850 0.55 -1.0 900,000 - LRK53_RS03850 0.55 -0.4 900,000 - LRK53_RS03850 0.55 -1.6 900,000 - LRK53_RS03850 0.55 -1.2 900,183 + LRK53_RS03850 0.88 -0.2 900,183 + LRK53_RS03850 0.88 -1.4 900,184 - LRK53_RS03850 0.88 -0.0 900,184 - LRK53_RS03850 0.88 -1.0 900,387 + +1.0 900,387 + -0.3 900,387 + -1.2 900,388 - +0.4 900,388 - -0.1 900,388 - +2.0 900,388 - -0.8 900,428 + +0.6 900,428 + +0.2 900,428 + -0.6 900,428 + +1.5 900,428 + -0.6 900,428 + -0.6 900,428 + -0.6 900,428 + +1.0 900,428 + +0.4 900,428 + +0.2 900,428 + +0.4 900,428 + -1.0 900,428 + +0.2 900,428 + -0.6 900,428 + +0.4 900,428 + -0.5 900,429 - -0.5 900,429 - -0.6 900,429 - -0.4 900,429 - +1.0 900,429 - +0.5 900,429 - +1.0 900,429 - -1.0 900,429 - +1.0 900,429 - -1.8 900,468 + LRK53_RS03855 0.10 +1.1 900,468 + LRK53_RS03855 0.10 +0.2 900,468 + LRK53_RS03855 0.10 +0.5 900,468 + LRK53_RS03855 0.10 -1.5 900,468 + LRK53_RS03855 0.10 +1.0 900,468 + LRK53_RS03855 0.10 -0.0 900,468 + LRK53_RS03855 0.10 -0.0 900,468 + LRK53_RS03855 0.10 -1.0 900,468 + LRK53_RS03855 0.10 -1.6 900,468 + LRK53_RS03855 0.10 -0.0 900,469 - LRK53_RS03855 0.10 -0.7 900,469 - LRK53_RS03855 0.10 -1.0 900,469 - LRK53_RS03855 0.10 -0.0 900,469 - LRK53_RS03855 0.10 -0.0 900,469 - LRK53_RS03855 0.10 -0.0 900,469 - LRK53_RS03855 0.10 +0.5 900,469 - LRK53_RS03855 0.10 -0.4 900,469 - LRK53_RS03855 0.10 -0.4 901,464 + LRK53_RS03855 0.57 -0.0 901,465 - LRK53_RS03855 0.57 -0.6 901,584 + LRK53_RS03855 0.63 -1.4 901,584 + LRK53_RS03855 0.63 -1.0 901,584 + LRK53_RS03855 0.63 +0.3 901,584 + LRK53_RS03855 0.63 -0.0 901,584 + LRK53_RS03855 0.63 +1.0 901,584 + LRK53_RS03855 0.63 +1.0 901,584 + LRK53_RS03855 0.63 -0.0 901,584 + LRK53_RS03855 0.63 -0.0 901,584 + LRK53_RS03855 0.63 +0.2 901,584 + LRK53_RS03855 0.63 -0.8 901,584 + LRK53_RS03855 0.63 -1.0 901,584 + LRK53_RS03855 0.63 +1.0 901,584 + LRK53_RS03855 0.63 -0.6 901,584 + LRK53_RS03855 0.63 +2.0 901,584 + LRK53_RS03855 0.63 -0.2 901,585 - LRK53_RS03855 0.63 -0.0 901,585 - LRK53_RS03855 0.63 +0.4 901,585 - LRK53_RS03855 0.63 +1.5 901,585 - LRK53_RS03855 0.63 -0.8 901,585 - LRK53_RS03855 0.63 -0.4 901,585 - LRK53_RS03855 0.63 -1.0 901,585 - LRK53_RS03855 0.63 +0.2 901,585 - LRK53_RS03855 0.63 -0.0 901,585 - LRK53_RS03855 0.63 +0.6 902,016 + LRK53_RS03855 0.83 -0.6 902,016 + LRK53_RS03855 0.83 +1.0 902,017 - LRK53_RS03855 0.84 +1.2 902,017 - LRK53_RS03855 0.84 -0.7 902,073 + LRK53_RS03855 0.86 +0.7 902,073 + LRK53_RS03855 0.86 +0.2 902,073 + LRK53_RS03855 0.86 +1.2 902,073 + LRK53_RS03855 0.86 -0.8 902,073 + LRK53_RS03855 0.86 -0.0 902,074 - LRK53_RS03855 0.86 +1.5 902,181 + +1.4 902,181 + -2.0 902,181 + -1.0 902,181 + -1.8 902,182 - -0.0 902,182 - +1.0 902,182 - +0.2 902,182 - -0.6 902,182 - -1.3 902,182 - -0.0 902,325 + -0.1 902,325 + -1.4 902,326 - -0.7 902,454 + +1.0 902,460 + -0.0 902,461 - -2.2 903,152 + LRK53_RS03860 0.19 +0.1 903,152 + LRK53_RS03860 0.19 +0.4 903,152 + LRK53_RS03860 0.19 -0.6 903,152 + LRK53_RS03860 0.19 +0.1 903,152 + LRK53_RS03860 0.19 -0.3 903,152 + LRK53_RS03860 0.19 -0.0 903,152 + LRK53_RS03860 0.19 +0.2 903,152 + LRK53_RS03860 0.19 -0.6 903,152 + LRK53_RS03860 0.19 -1.8 903,152 + LRK53_RS03860 0.19 -1.4 903,152 + LRK53_RS03860 0.19 -0.6 903,152 + LRK53_RS03860 0.19 +1.0 903,153 - LRK53_RS03860 0.19 +2.0 903,153 - LRK53_RS03860 0.19 -2.8 903,153 - LRK53_RS03860 0.19 -0.2 903,153 - LRK53_RS03860 0.19 +2.0 903,153 - LRK53_RS03860 0.19 -0.0 903,153 - LRK53_RS03860 0.19 -1.0 903,153 - LRK53_RS03860 0.19 -0.4 903,153 - LRK53_RS03860 0.19 -0.8 903,153 - LRK53_RS03860 0.19 -0.0 903,153 - LRK53_RS03860 0.19 +0.2 903,153 - LRK53_RS03860 0.19 -0.5 903,153 - LRK53_RS03860 0.19 +0.1 903,153 - LRK53_RS03860 0.19 -0.4 903,153 - LRK53_RS03860 0.19 -1.0 903,212 + LRK53_RS03860 0.21 -0.3 903,213 - LRK53_RS03860 0.22 -1.3 903,213 - LRK53_RS03860 0.22 -0.8 903,213 - LRK53_RS03860 0.22 -1.4 903,213 - LRK53_RS03860 0.22 +0.4 903,213 - LRK53_RS03860 0.22 +0.4 903,213 - LRK53_RS03860 0.22 -0.5 903,213 - LRK53_RS03860 0.22 -0.2 903,296 + LRK53_RS03860 0.24 -0.4 903,296 + LRK53_RS03860 0.24 -0.5 903,296 + LRK53_RS03860 0.24 +1.4 903,296 + LRK53_RS03860 0.24 -0.0 903,297 - LRK53_RS03860 0.24 -2.5 903,297 - LRK53_RS03860 0.24 +1.7 903,297 - LRK53_RS03860 0.24 +0.4 903,297 - LRK53_RS03860 0.24 -0.7 903,297 - LRK53_RS03860 0.24 -2.4 903,297 - LRK53_RS03860 0.24 -0.5 903,297 - LRK53_RS03860 0.24 +1.0 903,297 - LRK53_RS03860 0.24 -0.3
Or see this region's nucleotide sequence