Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03655 and LRK53_RS03660 are separated by 7 nucleotides LRK53_RS03660 and LRK53_RS03665 are separated by 289 nucleotides LRK53_RS03665 and LRK53_RS03670 are separated by 19 nucleotides
LRK53_RS03655: LRK53_RS03655 - DEAD/DEAH box helicase, at 859,400 to 862,417
_RS03655
LRK53_RS03660: LRK53_RS03660 - hypothetical protein, at 862,425 to 862,745
_RS03660
LRK53_RS03665: LRK53_RS03665 - HAD family hydrolase, at 863,035 to 863,718
_RS03665
LRK53_RS03670: LRK53_RS03670 - hypothetical protein, at 863,738 to 864,985
_RS03670
Position (kb) 862
863 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 861.461 kb on + strand, within LRK53_RS03655 at 861.461 kb on + strand, within LRK53_RS03655 at 861.461 kb on + strand, within LRK53_RS03655 at 861.461 kb on + strand, within LRK53_RS03655 at 861.461 kb on + strand, within LRK53_RS03655 at 861.461 kb on + strand, within LRK53_RS03655 at 861.462 kb on - strand, within LRK53_RS03655 at 861.462 kb on - strand, within LRK53_RS03655 at 861.462 kb on - strand, within LRK53_RS03655 at 861.462 kb on - strand, within LRK53_RS03655 at 861.572 kb on + strand, within LRK53_RS03655 at 861.573 kb on - strand, within LRK53_RS03655 at 861.573 kb on - strand, within LRK53_RS03655 at 861.573 kb on - strand, within LRK53_RS03655 at 861.573 kb on - strand, within LRK53_RS03655 at 861.740 kb on + strand, within LRK53_RS03655 at 862.038 kb on - strand, within LRK53_RS03655 at 862.038 kb on - strand, within LRK53_RS03655 at 862.178 kb on + strand at 862.612 kb on + strand, within LRK53_RS03660 at 862.612 kb on + strand, within LRK53_RS03660 at 862.612 kb on + strand, within LRK53_RS03660 at 862.612 kb on + strand, within LRK53_RS03660 at 862.612 kb on + strand, within LRK53_RS03660 at 862.613 kb on - strand, within LRK53_RS03660 at 862.613 kb on - strand, within LRK53_RS03660 at 862.613 kb on - strand, within LRK53_RS03660 at 862.613 kb on - strand, within LRK53_RS03660 at 862.613 kb on - strand, within LRK53_RS03660 at 862.613 kb on - strand, within LRK53_RS03660 at 862.613 kb on - strand, within LRK53_RS03660 at 862.613 kb on - strand, within LRK53_RS03660 at 862.613 kb on - strand, within LRK53_RS03660 at 862.613 kb on - strand, within LRK53_RS03660 at 862.777 kb on + strand at 862.778 kb on - strand at 862.778 kb on - strand at 862.802 kb on + strand at 862.802 kb on + strand at 862.802 kb on + strand at 862.803 kb on - strand at 862.803 kb on - strand at 862.803 kb on - strand at 862.803 kb on - strand at 862.898 kb on + strand at 862.898 kb on + strand at 863.090 kb on + strand at 863.091 kb on - strand at 863.091 kb on - strand at 863.091 kb on - strand at 863.277 kb on + strand, within LRK53_RS03665 at 863.278 kb on - strand, within LRK53_RS03665 at 863.311 kb on + strand, within LRK53_RS03665 at 863.311 kb on + strand, within LRK53_RS03665 at 863.312 kb on - strand, within LRK53_RS03665 at 863.411 kb on + strand, within LRK53_RS03665 at 863.411 kb on + strand, within LRK53_RS03665 at 863.411 kb on + strand, within LRK53_RS03665 at 863.412 kb on - strand, within LRK53_RS03665 at 863.412 kb on - strand, within LRK53_RS03665 at 863.647 kb on + strand, within LRK53_RS03665 at 863.647 kb on + strand, within LRK53_RS03665 at 863.647 kb on + strand, within LRK53_RS03665 at 863.647 kb on + strand, within LRK53_RS03665 at 863.647 kb on + strand, within LRK53_RS03665 at 863.647 kb on + strand, within LRK53_RS03665 at 863.647 kb on + strand, within LRK53_RS03665 at 863.647 kb on + strand, within LRK53_RS03665 at 863.648 kb on - strand, within LRK53_RS03665 at 863.648 kb on - strand, within LRK53_RS03665 at 863.648 kb on - strand, within LRK53_RS03665 at 863.648 kb on - strand, within LRK53_RS03665 at 863.648 kb on - strand, within LRK53_RS03665 at 863.648 kb on - strand, within LRK53_RS03665 at 863.648 kb on - strand, within LRK53_RS03665 at 863.648 kb on - strand, within LRK53_RS03665 at 863.657 kb on + strand at 863.657 kb on + strand at 863.658 kb on - strand at 863.737 kb on - strand at 863.737 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 861,461 + LRK53_RS03655 0.68 -0.0 861,461 + LRK53_RS03655 0.68 +1.0 861,461 + LRK53_RS03655 0.68 +0.4 861,461 + LRK53_RS03655 0.68 -0.0 861,461 + LRK53_RS03655 0.68 -2.0 861,461 + LRK53_RS03655 0.68 -1.0 861,462 - LRK53_RS03655 0.68 -0.3 861,462 - LRK53_RS03655 0.68 +0.2 861,462 - LRK53_RS03655 0.68 +1.2 861,462 - LRK53_RS03655 0.68 -0.5 861,572 + LRK53_RS03655 0.72 -0.3 861,573 - LRK53_RS03655 0.72 +1.0 861,573 - LRK53_RS03655 0.72 +1.0 861,573 - LRK53_RS03655 0.72 -1.2 861,573 - LRK53_RS03655 0.72 -0.3 861,740 + LRK53_RS03655 0.78 -0.3 862,038 - LRK53_RS03655 0.87 -0.6 862,038 - LRK53_RS03655 0.87 -1.4 862,178 + -0.6 862,612 + LRK53_RS03660 0.58 -0.2 862,612 + LRK53_RS03660 0.58 -0.3 862,612 + LRK53_RS03660 0.58 -0.6 862,612 + LRK53_RS03660 0.58 -0.0 862,612 + LRK53_RS03660 0.58 +1.0 862,613 - LRK53_RS03660 0.59 -0.2 862,613 - LRK53_RS03660 0.59 -1.2 862,613 - LRK53_RS03660 0.59 +1.0 862,613 - LRK53_RS03660 0.59 +2.0 862,613 - LRK53_RS03660 0.59 -1.0 862,613 - LRK53_RS03660 0.59 -1.8 862,613 - LRK53_RS03660 0.59 +1.0 862,613 - LRK53_RS03660 0.59 +0.7 862,613 - LRK53_RS03660 0.59 -0.5 862,613 - LRK53_RS03660 0.59 +1.0 862,777 + -0.4 862,778 - -0.8 862,778 - -1.5 862,802 + -2.0 862,802 + -1.6 862,802 + -1.2 862,803 - -2.5 862,803 - +1.0 862,803 - -0.6 862,803 - -0.0 862,898 + -1.3 862,898 + -2.3 863,090 + -1.8 863,091 - -1.6 863,091 - -1.6 863,091 - -0.0 863,277 + LRK53_RS03665 0.35 +1.0 863,278 - LRK53_RS03665 0.36 +1.0 863,311 + LRK53_RS03665 0.40 +0.3 863,311 + LRK53_RS03665 0.40 +0.4 863,312 - LRK53_RS03665 0.40 -0.6 863,411 + LRK53_RS03665 0.55 -0.3 863,411 + LRK53_RS03665 0.55 -1.1 863,411 + LRK53_RS03665 0.55 -0.6 863,412 - LRK53_RS03665 0.55 -0.6 863,412 - LRK53_RS03665 0.55 -1.0 863,647 + LRK53_RS03665 0.89 +0.4 863,647 + LRK53_RS03665 0.89 +1.3 863,647 + LRK53_RS03665 0.89 -0.6 863,647 + LRK53_RS03665 0.89 +1.0 863,647 + LRK53_RS03665 0.89 +0.6 863,647 + LRK53_RS03665 0.89 +1.0 863,647 + LRK53_RS03665 0.89 -0.0 863,647 + LRK53_RS03665 0.89 -1.8 863,648 - LRK53_RS03665 0.90 +1.4 863,648 - LRK53_RS03665 0.90 -0.7 863,648 - LRK53_RS03665 0.90 +0.4 863,648 - LRK53_RS03665 0.90 -0.6 863,648 - LRK53_RS03665 0.90 +1.0 863,648 - LRK53_RS03665 0.90 -0.6 863,648 - LRK53_RS03665 0.90 -0.2 863,648 - LRK53_RS03665 0.90 -0.0 863,657 + -3.9 863,657 + -0.0 863,658 - -1.0 863,737 - -0.9 863,737 - +0.2
Or see this region's nucleotide sequence