Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03585 and LRK53_RS03590 are separated by 125 nucleotides LRK53_RS03590 and LRK53_RS03595 are separated by 22 nucleotides LRK53_RS03595 and LRK53_RS03600 are separated by 110 nucleotides
LRK53_RS03585: LRK53_RS03585 - tryptophan 7-halogenase, at 847,331 to 848,641
_RS03585
LRK53_RS03590: LRK53_RS03590 - thymidine kinase, at 848,767 to 849,399
_RS03590
LRK53_RS03595: LRK53_RS03595 - hypothetical protein, at 849,422 to 850,189
_RS03595
LRK53_RS03600: LRK53_RS03600 - nitroreductase family protein, at 850,300 to 850,899
_RS03600
Position (kb)
848
849
850 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 847.814 kb on + strand, within LRK53_RS03585 at 847.815 kb on - strand, within LRK53_RS03585 at 847.964 kb on + strand, within LRK53_RS03585 at 847.964 kb on + strand, within LRK53_RS03585 at 847.964 kb on + strand, within LRK53_RS03585 at 847.964 kb on + strand, within LRK53_RS03585 at 847.965 kb on - strand, within LRK53_RS03585 at 847.965 kb on - strand, within LRK53_RS03585 at 848.045 kb on + strand, within LRK53_RS03585 at 848.089 kb on + strand, within LRK53_RS03585 at 848.089 kb on + strand, within LRK53_RS03585 at 848.089 kb on + strand, within LRK53_RS03585 at 848.089 kb on + strand, within LRK53_RS03585 at 848.089 kb on + strand, within LRK53_RS03585 at 848.089 kb on + strand, within LRK53_RS03585 at 848.090 kb on - strand, within LRK53_RS03585 at 848.090 kb on - strand, within LRK53_RS03585 at 848.090 kb on - strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.171 kb on + strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.172 kb on - strand, within LRK53_RS03585 at 848.177 kb on + strand, within LRK53_RS03585 at 848.264 kb on + strand, within LRK53_RS03585 at 848.264 kb on + strand, within LRK53_RS03585 at 848.264 kb on + strand, within LRK53_RS03585 at 848.265 kb on - strand, within LRK53_RS03585 at 848.265 kb on - strand, within LRK53_RS03585 at 848.265 kb on - strand, within LRK53_RS03585 at 848.265 kb on - strand, within LRK53_RS03585 at 848.265 kb on - strand, within LRK53_RS03585 at 848.549 kb on + strand at 848.549 kb on + strand at 848.550 kb on - strand at 848.550 kb on - strand at 848.639 kb on + strand at 848.639 kb on + strand at 848.640 kb on - strand at 848.640 kb on - strand at 848.646 kb on + strand at 848.654 kb on - strand at 848.654 kb on - strand at 848.738 kb on + strand at 848.739 kb on - strand at 848.739 kb on - strand at 848.739 kb on - strand at 848.745 kb on + strand at 848.752 kb on + strand at 848.753 kb on - strand at 848.753 kb on - strand at 848.779 kb on + strand at 848.779 kb on + strand at 848.779 kb on + strand at 848.779 kb on + strand at 848.779 kb on + strand at 848.779 kb on + strand at 848.780 kb on - strand at 848.780 kb on - strand at 848.780 kb on - strand at 848.780 kb on - strand at 848.780 kb on - strand at 848.785 kb on + strand at 848.785 kb on + strand at 848.785 kb on + strand at 848.785 kb on + strand at 848.785 kb on + strand at 848.785 kb on + strand at 848.785 kb on + strand at 848.785 kb on + strand at 848.785 kb on + strand at 848.785 kb on + strand at 848.785 kb on + strand at 848.785 kb on + strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.786 kb on - strand at 848.789 kb on - strand at 848.789 kb on - strand at 848.836 kb on + strand, within LRK53_RS03590 at 848.836 kb on + strand, within LRK53_RS03590 at 848.836 kb on + strand, within LRK53_RS03590 at 848.836 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.842 kb on + strand, within LRK53_RS03590 at 848.843 kb on - strand, within LRK53_RS03590 at 848.843 kb on - strand, within LRK53_RS03590 at 849.110 kb on - strand, within LRK53_RS03590 at 849.110 kb on - strand, within LRK53_RS03590 at 849.301 kb on + strand, within LRK53_RS03590 at 849.301 kb on + strand, within LRK53_RS03590 at 849.301 kb on + strand, within LRK53_RS03590 at 849.301 kb on + strand, within LRK53_RS03590 at 849.301 kb on + strand, within LRK53_RS03590 at 849.301 kb on + strand, within LRK53_RS03590 at 849.301 kb on + strand, within LRK53_RS03590 at 849.301 kb on + strand, within LRK53_RS03590 at 849.301 kb on + strand, within LRK53_RS03590 at 849.302 kb on - strand, within LRK53_RS03590
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 847,814 + LRK53_RS03585 0.37 -1.9 847,815 - LRK53_RS03585 0.37 -0.5 847,964 + LRK53_RS03585 0.48 -0.8 847,964 + LRK53_RS03585 0.48 -0.7 847,964 + LRK53_RS03585 0.48 +2.6 847,964 + LRK53_RS03585 0.48 +0.2 847,965 - LRK53_RS03585 0.48 -0.6 847,965 - LRK53_RS03585 0.48 -0.6 848,045 + LRK53_RS03585 0.54 +1.2 848,089 + LRK53_RS03585 0.58 -1.2 848,089 + LRK53_RS03585 0.58 -1.8 848,089 + LRK53_RS03585 0.58 +0.4 848,089 + LRK53_RS03585 0.58 +0.4 848,089 + LRK53_RS03585 0.58 +0.1 848,089 + LRK53_RS03585 0.58 -0.5 848,090 - LRK53_RS03585 0.58 +0.5 848,090 - LRK53_RS03585 0.58 -0.0 848,090 - LRK53_RS03585 0.58 -1.0 848,171 + LRK53_RS03585 0.64 -0.7 848,171 + LRK53_RS03585 0.64 +0.4 848,171 + LRK53_RS03585 0.64 -0.6 848,171 + LRK53_RS03585 0.64 -0.7 848,171 + LRK53_RS03585 0.64 -1.4 848,171 + LRK53_RS03585 0.64 +1.0 848,171 + LRK53_RS03585 0.64 -0.0 848,171 + LRK53_RS03585 0.64 -2.0 848,171 + LRK53_RS03585 0.64 -1.6 848,171 + LRK53_RS03585 0.64 -0.2 848,171 + LRK53_RS03585 0.64 +1.4 848,171 + LRK53_RS03585 0.64 -1.3 848,172 - LRK53_RS03585 0.64 -0.0 848,172 - LRK53_RS03585 0.64 -1.0 848,172 - LRK53_RS03585 0.64 -1.2 848,172 - LRK53_RS03585 0.64 +0.4 848,172 - LRK53_RS03585 0.64 -0.2 848,172 - LRK53_RS03585 0.64 -0.6 848,172 - LRK53_RS03585 0.64 +2.0 848,172 - LRK53_RS03585 0.64 +0.4 848,172 - LRK53_RS03585 0.64 -1.4 848,172 - LRK53_RS03585 0.64 -2.2 848,172 - LRK53_RS03585 0.64 -0.4 848,172 - LRK53_RS03585 0.64 +0.4 848,172 - LRK53_RS03585 0.64 +0.4 848,172 - LRK53_RS03585 0.64 -0.0 848,172 - LRK53_RS03585 0.64 -0.0 848,172 - LRK53_RS03585 0.64 -0.5 848,177 + LRK53_RS03585 0.65 -1.3 848,264 + LRK53_RS03585 0.71 +1.0 848,264 + LRK53_RS03585 0.71 -1.0 848,264 + LRK53_RS03585 0.71 -0.2 848,265 - LRK53_RS03585 0.71 +0.4 848,265 - LRK53_RS03585 0.71 +0.4 848,265 - LRK53_RS03585 0.71 -0.0 848,265 - LRK53_RS03585 0.71 +0.6 848,265 - LRK53_RS03585 0.71 -1.8 848,549 + -1.6 848,549 + +2.0 848,550 - -0.5 848,550 - -1.9 848,639 + +0.4 848,639 + -0.7 848,640 - -1.5 848,640 - -0.3 848,646 + +1.0 848,654 - +1.0 848,654 - +0.1 848,738 + -0.6 848,739 - +1.0 848,739 - -1.6 848,739 - -1.7 848,745 + -0.5 848,752 + +1.0 848,753 - -2.2 848,753 - +1.0 848,779 + -2.0 848,779 + +1.0 848,779 + +1.0 848,779 + -0.0 848,779 + -1.3 848,779 + -0.0 848,780 - +1.0 848,780 - -0.8 848,780 - +0.2 848,780 - +1.0 848,780 - -0.6 848,785 + -2.8 848,785 + -2.2 848,785 + +0.4 848,785 + -0.6 848,785 + -0.6 848,785 + -0.8 848,785 + +0.2 848,785 + +1.0 848,785 + -0.2 848,785 + +0.6 848,785 + -0.0 848,785 + -0.6 848,786 - -1.0 848,786 - -0.8 848,786 - -0.6 848,786 - -0.0 848,786 - +1.0 848,786 - +0.7 848,786 - +2.6 848,786 - +1.0 848,786 - -0.0 848,786 - +2.3 848,786 - +0.4 848,786 - -0.0 848,786 - -1.8 848,786 - -0.0 848,786 - +0.2 848,789 - -0.8 848,789 - -2.9 848,836 + LRK53_RS03590 0.11 -2.7 848,836 + LRK53_RS03590 0.11 +0.4 848,836 + LRK53_RS03590 0.11 +0.8 848,836 + LRK53_RS03590 0.11 -0.0 848,842 + LRK53_RS03590 0.12 -0.0 848,842 + LRK53_RS03590 0.12 -0.3 848,842 + LRK53_RS03590 0.12 -0.0 848,842 + LRK53_RS03590 0.12 +0.2 848,842 + LRK53_RS03590 0.12 +2.6 848,842 + LRK53_RS03590 0.12 +1.0 848,842 + LRK53_RS03590 0.12 +1.0 848,842 + LRK53_RS03590 0.12 -0.5 848,842 + LRK53_RS03590 0.12 +0.1 848,842 + LRK53_RS03590 0.12 -0.0 848,842 + LRK53_RS03590 0.12 +2.6 848,842 + LRK53_RS03590 0.12 +1.0 848,842 + LRK53_RS03590 0.12 +0.8 848,843 - LRK53_RS03590 0.12 -0.6 848,843 - LRK53_RS03590 0.12 +1.4 849,110 - LRK53_RS03590 0.54 -0.9 849,110 - LRK53_RS03590 0.54 +1.6 849,301 + LRK53_RS03590 0.84 -0.5 849,301 + LRK53_RS03590 0.84 -1.0 849,301 + LRK53_RS03590 0.84 -0.0 849,301 + LRK53_RS03590 0.84 -0.6 849,301 + LRK53_RS03590 0.84 -1.3 849,301 + LRK53_RS03590 0.84 -0.3 849,301 + LRK53_RS03590 0.84 -1.0 849,301 + LRK53_RS03590 0.84 -0.6 849,301 + LRK53_RS03590 0.84 +1.3 849,302 - LRK53_RS03590 0.85 -0.6
Or see this region's nucleotide sequence