Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02375 and LRK53_RS02380 are separated by 414 nucleotides LRK53_RS02380 and LRK53_RS02385 are separated by 7 nucleotides LRK53_RS02385 and LRK53_RS02390 are separated by 83 nucleotides
LRK53_RS02375: LRK53_RS02375 - shikimate dehydrogenase, at 545,739 to 546,569
_RS02375
LRK53_RS02380: LRK53_RS02380 - hypothetical protein, at 546,984 to 547,532
_RS02380
LRK53_RS02385: LRK53_RS02385 - gamma carbonic anhydrase family protein, at 547,540 to 548,073
_RS02385
LRK53_RS02390: LRK53_RS02390 - M3 family metallopeptidase, at 548,157 to 550,214
_RS02390
Position (kb) 546
547
548 Strain fitness (log2 ratio) -2
-1
0
1
2 at 546.265 kb on - strand, within LRK53_RS02375 at 546.294 kb on + strand, within LRK53_RS02375 at 546.294 kb on + strand, within LRK53_RS02375 at 546.294 kb on + strand, within LRK53_RS02375 at 546.295 kb on - strand, within LRK53_RS02375 at 546.295 kb on - strand, within LRK53_RS02375 at 546.295 kb on - strand, within LRK53_RS02375 at 546.295 kb on - strand, within LRK53_RS02375 at 546.295 kb on - strand, within LRK53_RS02375 at 546.295 kb on - strand, within LRK53_RS02375 at 546.295 kb on - strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.384 kb on + strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.385 kb on - strand, within LRK53_RS02375 at 546.396 kb on + strand, within LRK53_RS02375 at 546.396 kb on + strand, within LRK53_RS02375 at 546.396 kb on + strand, within LRK53_RS02375 at 546.396 kb on + strand, within LRK53_RS02375 at 546.397 kb on - strand, within LRK53_RS02375 at 546.397 kb on - strand, within LRK53_RS02375 at 546.397 kb on - strand, within LRK53_RS02375 at 546.397 kb on - strand, within LRK53_RS02375 at 546.540 kb on + strand at 546.540 kb on + strand at 546.540 kb on + strand at 546.540 kb on + strand at 546.540 kb on + strand at 546.540 kb on + strand at 546.540 kb on + strand at 546.541 kb on - strand at 546.541 kb on - strand at 546.541 kb on - strand at 546.541 kb on - strand at 546.550 kb on + strand at 547.017 kb on + strand at 547.017 kb on + strand at 547.017 kb on + strand at 547.017 kb on + strand at 547.017 kb on + strand at 547.017 kb on + strand at 547.017 kb on + strand at 547.017 kb on + strand at 547.017 kb on + strand at 547.017 kb on + strand at 547.017 kb on + strand at 547.017 kb on + strand at 547.018 kb on - strand at 547.018 kb on - strand at 547.018 kb on - strand at 547.018 kb on - strand at 547.018 kb on - strand at 547.112 kb on + strand, within LRK53_RS02380 at 547.112 kb on + strand, within LRK53_RS02380 at 547.112 kb on + strand, within LRK53_RS02380 at 547.113 kb on - strand, within LRK53_RS02380 at 547.251 kb on + strand, within LRK53_RS02380 at 547.251 kb on + strand, within LRK53_RS02380 at 547.251 kb on + strand, within LRK53_RS02380 at 547.251 kb on + strand, within LRK53_RS02380 at 547.251 kb on + strand, within LRK53_RS02380 at 547.251 kb on + strand, within LRK53_RS02380 at 547.251 kb on + strand, within LRK53_RS02380 at 547.533 kb on + strand at 547.556 kb on + strand at 547.556 kb on + strand at 547.556 kb on + strand at 547.557 kb on - strand at 547.557 kb on - strand at 547.557 kb on - strand at 547.557 kb on - strand at 547.557 kb on - strand at 547.577 kb on + strand at 547.577 kb on + strand at 547.577 kb on + strand at 547.577 kb on + strand at 547.577 kb on + strand at 547.577 kb on + strand at 547.578 kb on - strand at 547.578 kb on - strand at 547.578 kb on - strand at 547.578 kb on - strand at 547.578 kb on - strand at 547.578 kb on - strand at 547.578 kb on - strand at 547.578 kb on - strand at 547.578 kb on - strand at 547.578 kb on - strand at 547.592 kb on + strand at 547.593 kb on - strand at 547.593 kb on - strand at 547.772 kb on + strand, within LRK53_RS02385 at 547.772 kb on + strand, within LRK53_RS02385 at 547.772 kb on + strand, within LRK53_RS02385 at 547.772 kb on + strand, within LRK53_RS02385 at 547.773 kb on - strand, within LRK53_RS02385 at 547.773 kb on - strand, within LRK53_RS02385 at 547.773 kb on - strand, within LRK53_RS02385 at 547.856 kb on + strand, within LRK53_RS02385 at 547.923 kb on + strand, within LRK53_RS02385 at 547.923 kb on + strand, within LRK53_RS02385 at 547.923 kb on + strand, within LRK53_RS02385 at 547.924 kb on - strand, within LRK53_RS02385 at 548.085 kb on - strand at 548.259 kb on + strand at 548.260 kb on - strand at 548.260 kb on - strand at 548.419 kb on - strand, within LRK53_RS02390 at 548.481 kb on + strand, within LRK53_RS02390 at 548.481 kb on + strand, within LRK53_RS02390 at 548.481 kb on + strand, within LRK53_RS02390 at 548.482 kb on - strand, within LRK53_RS02390 at 548.482 kb on - strand, within LRK53_RS02390
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 546,265 - LRK53_RS02375 0.63 +0.0 546,294 + LRK53_RS02375 0.67 -1.1 546,294 + LRK53_RS02375 0.67 -1.8 546,294 + LRK53_RS02375 0.67 -0.6 546,295 - LRK53_RS02375 0.67 +0.6 546,295 - LRK53_RS02375 0.67 +1.0 546,295 - LRK53_RS02375 0.67 -1.3 546,295 - LRK53_RS02375 0.67 +1.0 546,295 - LRK53_RS02375 0.67 -0.7 546,295 - LRK53_RS02375 0.67 +0.2 546,295 - LRK53_RS02375 0.67 -0.5 546,384 + LRK53_RS02375 0.78 -0.8 546,384 + LRK53_RS02375 0.78 -1.0 546,384 + LRK53_RS02375 0.78 -0.6 546,384 + LRK53_RS02375 0.78 -1.8 546,384 + LRK53_RS02375 0.78 -0.8 546,384 + LRK53_RS02375 0.78 +0.4 546,384 + LRK53_RS02375 0.78 +1.6 546,384 + LRK53_RS02375 0.78 +2.6 546,384 + LRK53_RS02375 0.78 -0.1 546,384 + LRK53_RS02375 0.78 +2.0 546,384 + LRK53_RS02375 0.78 +0.7 546,384 + LRK53_RS02375 0.78 -1.3 546,385 - LRK53_RS02375 0.78 +1.0 546,385 - LRK53_RS02375 0.78 -0.7 546,385 - LRK53_RS02375 0.78 +1.0 546,385 - LRK53_RS02375 0.78 -1.7 546,385 - LRK53_RS02375 0.78 -1.0 546,385 - LRK53_RS02375 0.78 +0.0 546,385 - LRK53_RS02375 0.78 -0.2 546,385 - LRK53_RS02375 0.78 +0.6 546,385 - LRK53_RS02375 0.78 +0.7 546,385 - LRK53_RS02375 0.78 -1.2 546,385 - LRK53_RS02375 0.78 -1.0 546,385 - LRK53_RS02375 0.78 +0.3 546,385 - LRK53_RS02375 0.78 -1.2 546,385 - LRK53_RS02375 0.78 -0.3 546,385 - LRK53_RS02375 0.78 -0.8 546,385 - LRK53_RS02375 0.78 +2.0 546,385 - LRK53_RS02375 0.78 +0.0 546,385 - LRK53_RS02375 0.78 +0.0 546,385 - LRK53_RS02375 0.78 -0.6 546,385 - LRK53_RS02375 0.78 -0.7 546,385 - LRK53_RS02375 0.78 -1.3 546,396 + LRK53_RS02375 0.79 -1.2 546,396 + LRK53_RS02375 0.79 +1.0 546,396 + LRK53_RS02375 0.79 -1.8 546,396 + LRK53_RS02375 0.79 -0.5 546,397 - LRK53_RS02375 0.79 +0.4 546,397 - LRK53_RS02375 0.79 -1.3 546,397 - LRK53_RS02375 0.79 -0.2 546,397 - LRK53_RS02375 0.79 +0.0 546,540 + -2.7 546,540 + +2.0 546,540 + +0.4 546,540 + -0.6 546,540 + -0.4 546,540 + -1.0 546,540 + -0.3 546,541 - +0.3 546,541 - +0.0 546,541 - -1.3 546,541 - +0.0 546,550 + -1.6 547,017 + -1.1 547,017 + -1.0 547,017 + -1.0 547,017 + -0.6 547,017 + -0.6 547,017 + +0.0 547,017 + +0.0 547,017 + -0.6 547,017 + +2.6 547,017 + +0.3 547,017 + -0.3 547,017 + -0.7 547,018 - +1.2 547,018 - +0.0 547,018 - +2.0 547,018 - -0.6 547,018 - +1.0 547,112 + LRK53_RS02380 0.23 -0.2 547,112 + LRK53_RS02380 0.23 -1.0 547,112 + LRK53_RS02380 0.23 -1.4 547,113 - LRK53_RS02380 0.23 -1.0 547,251 + LRK53_RS02380 0.49 -0.3 547,251 + LRK53_RS02380 0.49 -1.6 547,251 + LRK53_RS02380 0.49 +0.0 547,251 + LRK53_RS02380 0.49 -0.6 547,251 + LRK53_RS02380 0.49 +0.0 547,251 + LRK53_RS02380 0.49 -1.0 547,251 + LRK53_RS02380 0.49 +0.0 547,533 + +0.0 547,556 + +0.8 547,556 + -0.6 547,556 + +0.4 547,557 - -0.7 547,557 - -0.6 547,557 - -0.8 547,557 - +0.4 547,557 - -1.2 547,577 + -0.3 547,577 + -2.0 547,577 + -0.4 547,577 + -2.0 547,577 + -1.0 547,577 + +0.2 547,578 - +0.2 547,578 - +0.4 547,578 - -0.6 547,578 - -0.6 547,578 - -1.6 547,578 - +1.0 547,578 - -2.0 547,578 - -1.4 547,578 - +0.6 547,578 - +2.6 547,592 + +1.0 547,593 - -0.6 547,593 - -0.9 547,772 + LRK53_RS02385 0.43 +1.0 547,772 + LRK53_RS02385 0.43 -0.5 547,772 + LRK53_RS02385 0.43 -1.1 547,772 + LRK53_RS02385 0.43 +1.0 547,773 - LRK53_RS02385 0.44 -2.0 547,773 - LRK53_RS02385 0.44 -0.3 547,773 - LRK53_RS02385 0.44 +0.0 547,856 + LRK53_RS02385 0.59 -1.3 547,923 + LRK53_RS02385 0.72 -0.6 547,923 + LRK53_RS02385 0.72 -0.1 547,923 + LRK53_RS02385 0.72 -0.3 547,924 - LRK53_RS02385 0.72 -0.6 548,085 - +2.0 548,259 + +0.0 548,260 - +1.0 548,260 - +0.0 548,419 - LRK53_RS02390 0.13 +1.0 548,481 + LRK53_RS02390 0.16 +1.0 548,481 + LRK53_RS02390 0.16 -1.3 548,481 + LRK53_RS02390 0.16 +0.4 548,482 - LRK53_RS02390 0.16 -1.0 548,482 - LRK53_RS02390 0.16 -0.3
Or see this region's nucleotide sequence