Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02345 and LRK53_RS02350 are separated by 28 nucleotides LRK53_RS02350 and LRK53_RS02355 overlap by 1 nucleotides
LRK53_RS02345: LRK53_RS02345 - hydroxymethylglutaryl-CoA reductase, degradative, at 538,535 to 540,874
_RS02345
LRK53_RS02350: LRK53_RS02350 - hypothetical protein, at 540,903 to 541,922
_RS02350
LRK53_RS02355: LRK53_RS02355 - diphosphomevalonate decarboxylase, at 541,922 to 542,914
_RS02355
Position (kb)
540
541
542 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 540.241 kb on + strand, within LRK53_RS02345 at 540.241 kb on + strand, within LRK53_RS02345 at 540.242 kb on - strand, within LRK53_RS02345 at 540.242 kb on - strand, within LRK53_RS02345 at 540.242 kb on - strand, within LRK53_RS02345 at 540.302 kb on + strand, within LRK53_RS02345 at 540.302 kb on + strand, within LRK53_RS02345 at 540.302 kb on + strand, within LRK53_RS02345 at 540.302 kb on + strand, within LRK53_RS02345 at 540.302 kb on + strand, within LRK53_RS02345 at 540.302 kb on + strand, within LRK53_RS02345 at 540.302 kb on + strand, within LRK53_RS02345 at 540.302 kb on + strand, within LRK53_RS02345 at 540.302 kb on + strand, within LRK53_RS02345 at 540.303 kb on - strand, within LRK53_RS02345 at 540.303 kb on - strand, within LRK53_RS02345 at 540.303 kb on - strand, within LRK53_RS02345 at 540.303 kb on - strand, within LRK53_RS02345 at 540.303 kb on - strand, within LRK53_RS02345 at 540.303 kb on - strand, within LRK53_RS02345 at 540.303 kb on - strand, within LRK53_RS02345 at 540.303 kb on - strand, within LRK53_RS02345 at 540.303 kb on - strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.561 kb on + strand, within LRK53_RS02345 at 540.562 kb on - strand, within LRK53_RS02345 at 540.562 kb on - strand, within LRK53_RS02345 at 540.562 kb on - strand, within LRK53_RS02345 at 540.562 kb on - strand, within LRK53_RS02345 at 540.562 kb on - strand, within LRK53_RS02345 at 540.562 kb on - strand, within LRK53_RS02345 at 540.562 kb on - strand, within LRK53_RS02345 at 540.562 kb on - strand, within LRK53_RS02345 at 540.890 kb on + strand at 540.890 kb on + strand at 540.890 kb on + strand at 540.890 kb on + strand at 540.890 kb on + strand at 540.890 kb on + strand at 540.891 kb on - strand at 540.891 kb on - strand at 540.891 kb on - strand at 540.939 kb on + strand at 540.940 kb on - strand at 540.940 kb on - strand at 541.054 kb on + strand, within LRK53_RS02350 at 541.054 kb on + strand, within LRK53_RS02350 at 541.054 kb on + strand, within LRK53_RS02350 at 541.054 kb on + strand, within LRK53_RS02350 at 541.054 kb on + strand, within LRK53_RS02350 at 541.054 kb on + strand, within LRK53_RS02350 at 541.055 kb on - strand, within LRK53_RS02350 at 541.055 kb on - strand, within LRK53_RS02350 at 541.055 kb on - strand, within LRK53_RS02350 at 541.055 kb on - strand, within LRK53_RS02350 at 541.055 kb on - strand, within LRK53_RS02350 at 541.055 kb on - strand, within LRK53_RS02350 at 541.055 kb on - strand, within LRK53_RS02350 at 541.055 kb on - strand, within LRK53_RS02350 at 541.055 kb on - strand, within LRK53_RS02350 at 541.394 kb on - strand, within LRK53_RS02350 at 541.786 kb on + strand, within LRK53_RS02350 at 541.873 kb on + strand at 541.873 kb on + strand at 541.873 kb on + strand at 541.873 kb on + strand at 541.874 kb on - strand at 542.154 kb on + strand, within LRK53_RS02355 at 542.155 kb on - strand, within LRK53_RS02355 at 542.155 kb on - strand, within LRK53_RS02355 at 542.155 kb on - strand, within LRK53_RS02355 at 542.155 kb on - strand, within LRK53_RS02355 at 542.304 kb on + strand, within LRK53_RS02355 at 542.304 kb on + strand, within LRK53_RS02355 at 542.305 kb on - strand, within LRK53_RS02355 at 542.868 kb on + strand at 542.868 kb on + strand at 542.868 kb on + strand at 542.868 kb on + strand at 542.868 kb on + strand at 542.868 kb on + strand at 542.869 kb on - strand at 542.869 kb on - strand at 542.869 kb on - strand at 542.869 kb on - strand at 542.869 kb on - strand at 542.869 kb on - strand at 542.869 kb on - strand at 542.869 kb on - strand at 542.869 kb on - strand at 542.869 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 540,241 + LRK53_RS02345 0.73 -1.0 540,241 + LRK53_RS02345 0.73 -0.8 540,242 - LRK53_RS02345 0.73 -1.0 540,242 - LRK53_RS02345 0.73 -0.1 540,242 - LRK53_RS02345 0.73 +0.0 540,302 + LRK53_RS02345 0.76 -0.4 540,302 + LRK53_RS02345 0.76 -0.6 540,302 + LRK53_RS02345 0.76 -1.3 540,302 + LRK53_RS02345 0.76 +0.1 540,302 + LRK53_RS02345 0.76 +1.0 540,302 + LRK53_RS02345 0.76 +0.2 540,302 + LRK53_RS02345 0.76 -0.9 540,302 + LRK53_RS02345 0.76 -0.8 540,302 + LRK53_RS02345 0.76 -0.2 540,303 - LRK53_RS02345 0.76 -0.4 540,303 - LRK53_RS02345 0.76 -0.2 540,303 - LRK53_RS02345 0.76 +0.4 540,303 - LRK53_RS02345 0.76 -1.6 540,303 - LRK53_RS02345 0.76 -0.6 540,303 - LRK53_RS02345 0.76 +0.4 540,303 - LRK53_RS02345 0.76 -1.6 540,303 - LRK53_RS02345 0.76 -1.5 540,303 - LRK53_RS02345 0.76 -1.0 540,561 + LRK53_RS02345 0.87 +0.0 540,561 + LRK53_RS02345 0.87 -0.6 540,561 + LRK53_RS02345 0.87 +0.7 540,561 + LRK53_RS02345 0.87 +0.6 540,561 + LRK53_RS02345 0.87 +1.0 540,561 + LRK53_RS02345 0.87 +1.0 540,561 + LRK53_RS02345 0.87 -1.4 540,561 + LRK53_RS02345 0.87 -1.0 540,561 + LRK53_RS02345 0.87 +1.0 540,561 + LRK53_RS02345 0.87 +1.1 540,561 + LRK53_RS02345 0.87 +0.7 540,561 + LRK53_RS02345 0.87 -1.0 540,561 + LRK53_RS02345 0.87 -0.8 540,561 + LRK53_RS02345 0.87 -1.3 540,562 - LRK53_RS02345 0.87 +0.3 540,562 - LRK53_RS02345 0.87 +0.0 540,562 - LRK53_RS02345 0.87 +1.0 540,562 - LRK53_RS02345 0.87 +1.0 540,562 - LRK53_RS02345 0.87 +0.4 540,562 - LRK53_RS02345 0.87 -1.8 540,562 - LRK53_RS02345 0.87 -0.4 540,562 - LRK53_RS02345 0.87 +0.0 540,890 + +1.4 540,890 + +0.0 540,890 + -2.0 540,890 + -1.0 540,890 + +1.6 540,890 + +0.0 540,891 - +0.0 540,891 - -0.3 540,891 - -0.8 540,939 + +0.0 540,940 - +0.0 540,940 - -0.8 541,054 + LRK53_RS02350 0.15 +1.0 541,054 + LRK53_RS02350 0.15 -1.0 541,054 + LRK53_RS02350 0.15 -0.3 541,054 + LRK53_RS02350 0.15 +1.0 541,054 + LRK53_RS02350 0.15 +0.0 541,054 + LRK53_RS02350 0.15 -0.3 541,055 - LRK53_RS02350 0.15 +1.0 541,055 - LRK53_RS02350 0.15 +1.0 541,055 - LRK53_RS02350 0.15 +1.0 541,055 - LRK53_RS02350 0.15 +0.4 541,055 - LRK53_RS02350 0.15 +0.6 541,055 - LRK53_RS02350 0.15 +0.0 541,055 - LRK53_RS02350 0.15 +0.0 541,055 - LRK53_RS02350 0.15 -0.2 541,055 - LRK53_RS02350 0.15 -1.0 541,394 - LRK53_RS02350 0.48 -1.3 541,786 + LRK53_RS02350 0.87 +0.0 541,873 + -0.6 541,873 + +1.0 541,873 + +1.0 541,873 + +1.7 541,874 - +0.3 542,154 + LRK53_RS02355 0.23 -1.3 542,155 - LRK53_RS02355 0.23 -2.4 542,155 - LRK53_RS02355 0.23 +2.0 542,155 - LRK53_RS02355 0.23 -0.8 542,155 - LRK53_RS02355 0.23 -1.1 542,304 + LRK53_RS02355 0.38 +1.0 542,304 + LRK53_RS02355 0.38 -1.6 542,305 - LRK53_RS02355 0.39 -1.5 542,868 + +0.0 542,868 + +0.0 542,868 + +0.3 542,868 + -2.0 542,868 + +0.1 542,868 + -1.0 542,869 - +1.0 542,869 - +0.8 542,869 - -1.9 542,869 - -0.6 542,869 - -0.5 542,869 - +1.0 542,869 - +0.0 542,869 - +1.0 542,869 - +0.0 542,869 - -1.0
Or see this region's nucleotide sequence