Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02305 and LRK53_RS02310 are separated by 122 nucleotides LRK53_RS02310 and LRK53_RS02315 are separated by 185 nucleotides LRK53_RS02315 and LRK53_RS02320 are separated by 7 nucleotides LRK53_RS02320 and LRK53_RS02325 are separated by 94 nucleotides
LRK53_RS02305: LRK53_RS02305 - ATP-dependent protease ATPase subunit HslU, at 532,282 to 533,616
_RS02305
LRK53_RS02310: LRK53_RS02310 - ATP-dependent protease subunit HslV, at 533,739 to 534,275
_RS02310
LRK53_RS02315: LRK53_RS02315 - tyrosine recombinase XerC, at 534,461 to 535,348
_RS02315
LRK53_RS02320: LRK53_RS02320 - DUF484 family protein, at 535,356 to 536,042
_RS02320
LRK53_RS02325: LRK53_RS02325 - diaminopimelate epimerase, at 536,137 to 536,982
_RS02325
Position (kb) 534
535
536 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 533.570 kb on + strand at 533.571 kb on - strand at 533.571 kb on - strand at 533.571 kb on - strand at 533.571 kb on - strand at 533.571 kb on - strand at 533.571 kb on - strand at 533.571 kb on - strand at 533.571 kb on - strand at 533.687 kb on + strand at 533.687 kb on + strand at 533.687 kb on + strand at 533.688 kb on - strand at 533.688 kb on - strand at 533.740 kb on + strand at 533.740 kb on + strand at 533.740 kb on + strand at 533.740 kb on + strand at 533.740 kb on + strand at 533.741 kb on - strand at 533.741 kb on - strand at 533.741 kb on - strand at 533.741 kb on - strand at 533.741 kb on - strand at 533.741 kb on - strand at 533.741 kb on - strand at 533.741 kb on - strand at 533.741 kb on - strand at 533.741 kb on - strand at 533.741 kb on - strand at 533.773 kb on + strand at 533.773 kb on + strand at 533.773 kb on + strand at 533.773 kb on + strand at 533.773 kb on + strand at 533.773 kb on + strand at 533.773 kb on + strand at 533.774 kb on - strand at 533.774 kb on - strand at 533.774 kb on - strand at 533.774 kb on - strand at 533.774 kb on - strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.498 kb on + strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.499 kb on - strand at 534.532 kb on + strand at 534.532 kb on + strand at 534.533 kb on - strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.534 kb on + strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.535 kb on - strand at 534.851 kb on + strand, within LRK53_RS02315 at 534.851 kb on + strand, within LRK53_RS02315 at 534.851 kb on + strand, within LRK53_RS02315 at 534.851 kb on + strand, within LRK53_RS02315 at 534.852 kb on - strand, within LRK53_RS02315 at 534.852 kb on - strand, within LRK53_RS02315 at 534.852 kb on - strand, within LRK53_RS02315 at 534.852 kb on - strand, within LRK53_RS02315 at 534.924 kb on + strand, within LRK53_RS02315 at 534.924 kb on + strand, within LRK53_RS02315 at 534.924 kb on + strand, within LRK53_RS02315 at 534.925 kb on - strand, within LRK53_RS02315 at 534.925 kb on - strand, within LRK53_RS02315 at 534.925 kb on - strand, within LRK53_RS02315 at 534.925 kb on - strand, within LRK53_RS02315 at 534.925 kb on - strand, within LRK53_RS02315 at 535.050 kb on - strand, within LRK53_RS02315 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.891 kb on + strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.892 kb on - strand, within LRK53_RS02320 at 535.990 kb on + strand at 535.990 kb on + strand at 535.990 kb on + strand at 535.990 kb on + strand at 535.991 kb on - strand at 535.991 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 64 uM remove 533,570 + +0.0 533,571 - -1.6 533,571 - +0.0 533,571 - +0.3 533,571 - +0.4 533,571 - -1.3 533,571 - -0.6 533,571 - -0.8 533,571 - -1.0 533,687 + -0.6 533,687 + -1.0 533,687 + -1.3 533,688 - +0.6 533,688 - -1.3 533,740 + -0.3 533,740 + +0.0 533,740 + +0.0 533,740 + +0.4 533,740 + -0.6 533,741 - -0.2 533,741 - -1.8 533,741 - -0.4 533,741 - -0.8 533,741 - +0.9 533,741 - -0.3 533,741 - +0.0 533,741 - +1.0 533,741 - -0.6 533,741 - -1.0 533,741 - -0.5 533,773 + +1.0 533,773 + -2.3 533,773 + +0.7 533,773 + +0.0 533,773 + +1.0 533,773 + +0.0 533,773 + +0.4 533,774 - +1.0 533,774 - +1.0 533,774 - -1.1 533,774 - +1.3 533,774 - +1.0 534,498 + -2.6 534,498 + +0.3 534,498 + -1.1 534,498 + +0.0 534,498 + +0.0 534,498 + -0.6 534,498 + +1.0 534,498 + -1.3 534,498 + -1.2 534,498 + -0.3 534,498 + -1.0 534,498 + -1.0 534,498 + -0.9 534,499 - -1.6 534,499 - -0.9 534,499 - +0.0 534,499 - -1.3 534,499 - -1.0 534,499 - +3.0 534,499 - +1.0 534,499 - +0.0 534,499 - -1.4 534,499 - -1.0 534,499 - -2.2 534,499 - -1.0 534,499 - -1.0 534,532 + -0.6 534,532 + -1.2 534,533 - -0.4 534,534 + -0.6 534,534 + +0.0 534,534 + -1.2 534,534 + -2.5 534,534 + +0.0 534,534 + +0.0 534,534 + -0.3 534,534 + -1.0 534,534 + +0.0 534,534 + -0.6 534,534 + +1.0 534,534 + -0.6 534,534 + +2.0 534,534 + -2.2 534,534 + +0.2 534,534 + -1.5 534,534 + +0.0 534,534 + +1.0 534,535 - -1.0 534,535 - -1.0 534,535 - -2.1 534,535 - -0.4 534,535 - -2.0 534,535 - +0.4 534,535 - -1.6 534,535 - +0.3 534,535 - +0.4 534,535 - +0.4 534,535 - +0.2 534,535 - -1.0 534,535 - -1.1 534,535 - -0.6 534,535 - -1.6 534,535 - +1.0 534,535 - -1.3 534,535 - -0.8 534,535 - -1.1 534,535 - -0.5 534,535 - -1.0 534,535 - +1.0 534,535 - -1.3 534,535 - -2.2 534,535 - -1.6 534,535 - +0.4 534,535 - -1.6 534,851 + LRK53_RS02315 0.44 -0.7 534,851 + LRK53_RS02315 0.44 +0.6 534,851 + LRK53_RS02315 0.44 -1.5 534,851 + LRK53_RS02315 0.44 -1.5 534,852 - LRK53_RS02315 0.44 -0.6 534,852 - LRK53_RS02315 0.44 -1.0 534,852 - LRK53_RS02315 0.44 -1.3 534,852 - LRK53_RS02315 0.44 -0.7 534,924 + LRK53_RS02315 0.52 +0.0 534,924 + LRK53_RS02315 0.52 -1.0 534,924 + LRK53_RS02315 0.52 +0.8 534,925 - LRK53_RS02315 0.52 -1.6 534,925 - LRK53_RS02315 0.52 -0.9 534,925 - LRK53_RS02315 0.52 -1.7 534,925 - LRK53_RS02315 0.52 -1.3 534,925 - LRK53_RS02315 0.52 -1.9 535,050 - LRK53_RS02315 0.66 +1.0 535,891 + LRK53_RS02320 0.78 -1.6 535,891 + LRK53_RS02320 0.78 -1.8 535,891 + LRK53_RS02320 0.78 -2.9 535,891 + LRK53_RS02320 0.78 +1.0 535,891 + LRK53_RS02320 0.78 -0.3 535,891 + LRK53_RS02320 0.78 +0.0 535,891 + LRK53_RS02320 0.78 -0.2 535,891 + LRK53_RS02320 0.78 +1.0 535,891 + LRK53_RS02320 0.78 +1.0 535,891 + LRK53_RS02320 0.78 -0.3 535,891 + LRK53_RS02320 0.78 +1.0 535,891 + LRK53_RS02320 0.78 -0.9 535,891 + LRK53_RS02320 0.78 -2.7 535,892 - LRK53_RS02320 0.78 -2.1 535,892 - LRK53_RS02320 0.78 -0.2 535,892 - LRK53_RS02320 0.78 -1.1 535,892 - LRK53_RS02320 0.78 +1.3 535,892 - LRK53_RS02320 0.78 +1.6 535,892 - LRK53_RS02320 0.78 -1.8 535,892 - LRK53_RS02320 0.78 -0.5 535,892 - LRK53_RS02320 0.78 -0.6 535,892 - LRK53_RS02320 0.78 -0.8 535,892 - LRK53_RS02320 0.78 -2.0 535,892 - LRK53_RS02320 0.78 -0.5 535,892 - LRK53_RS02320 0.78 -2.0 535,892 - LRK53_RS02320 0.78 +1.0 535,990 + +0.0 535,990 + -1.6 535,990 + +1.0 535,990 + +1.0 535,991 - +0.0 535,991 - -0.6
Or see this region's nucleotide sequence