Experiment: WM_T2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JDDGAC_02430 and JDDGAC_02435 are separated by 3 nucleotides JDDGAC_02435 and JDDGAC_02440 are separated by 92 nucleotides JDDGAC_02440 and parE are separated by 430 nucleotides parE and JDDGAC_02450 are separated by 1 nucleotides JDDGAC_02450 and JDDGAC_02455 are separated by 20 nucleotides JDDGAC_02455 and ydaS are separated by 97 nucleotides ydaS and JDDGAC_02465 overlap by 4 nucleotides
JDDGAC_02430: JDDGAC_02430 - hypothetical protein, at 440,058 to 440,222
_02430
JDDGAC_02435: JDDGAC_02435 - Uncharacterized protein YdfC, at 440,226 to 440,444
_02435
JDDGAC_02440: JDDGAC_02440 - hypothetical protein, at 440,537 to 440,737
_02440
JDDGAC_02445: parE - plasmid stabilization protein ParE, at 441,168 to 441,446
parE
JDDGAC_02450: JDDGAC_02450 - stability determinant, at 441,448 to 441,639
_02450
JDDGAC_02455: JDDGAC_02455 - Cro/Cl family transcriptional regulator, at 441,660 to 442,031
_02455
JDDGAC_02460: ydaS - Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT, at 442,129 to 442,431
ydaS
JDDGAC_02465: JDDGAC_02465 - Rha family transcriptional regulator, at 442,428 to 442,853
_02465
Position (kb) 441
442 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 440.207 kb on + strand at 440.237 kb on - strand at 440.774 kb on + strand at 440.806 kb on - strand at 440.983 kb on + strand at 441.050 kb on - strand at 441.107 kb on - strand at 441.146 kb on - strand at 441.205 kb on - strand, within parE at 441.243 kb on - strand, within parE at 441.249 kb on - strand, within parE at 441.249 kb on - strand, within parE at 441.279 kb on + strand, within parE at 441.304 kb on + strand, within parE at 441.369 kb on + strand, within parE at 441.389 kb on - strand, within parE at 441.389 kb on - strand, within parE at 441.393 kb on - strand, within parE at 441.393 kb on - strand, within parE at 441.396 kb on + strand, within parE at 441.397 kb on - strand, within parE at 441.398 kb on + strand, within parE at 441.406 kb on - strand, within parE at 441.406 kb on - strand, within parE at 441.413 kb on + strand, within parE at 441.657 kb on - strand at 442.128 kb on + strand at 442.210 kb on + strand, within ydaS at 442.211 kb on - strand, within ydaS at 442.211 kb on - strand, within ydaS at 442.242 kb on + strand, within ydaS at 442.243 kb on - strand, within ydaS at 442.243 kb on - strand, within ydaS at 442.292 kb on + strand, within ydaS at 442.293 kb on - strand, within ydaS at 442.329 kb on + strand, within ydaS at 442.330 kb on - strand, within ydaS at 442.376 kb on + strand, within ydaS at 442.398 kb on + strand, within ydaS at 442.398 kb on + strand, within ydaS
Per-strain Table
Position Strand Gene LocusTag Fraction WM_T2 remove 440,207 + +0.8 440,237 - +0.2 440,774 + -1.9 440,806 - +0.3 440,983 + -1.9 441,050 - +0.9 441,107 - -1.4 441,146 - +1.1 441,205 - parE JDDGAC_02445 0.13 +0.7 441,243 - parE JDDGAC_02445 0.27 -1.0 441,249 - parE JDDGAC_02445 0.29 +0.7 441,249 - parE JDDGAC_02445 0.29 -1.7 441,279 + parE JDDGAC_02445 0.40 +0.4 441,304 + parE JDDGAC_02445 0.49 +0.6 441,369 + parE JDDGAC_02445 0.72 +1.7 441,389 - parE JDDGAC_02445 0.79 +0.4 441,389 - parE JDDGAC_02445 0.79 +1.2 441,393 - parE JDDGAC_02445 0.81 +0.4 441,393 - parE JDDGAC_02445 0.81 +1.0 441,396 + parE JDDGAC_02445 0.82 -0.3 441,397 - parE JDDGAC_02445 0.82 +0.7 441,398 + parE JDDGAC_02445 0.82 -2.8 441,406 - parE JDDGAC_02445 0.85 +0.1 441,406 - parE JDDGAC_02445 0.85 +0.3 441,413 + parE JDDGAC_02445 0.88 +2.3 441,657 - +0.3 442,128 + +1.2 442,210 + ydaS JDDGAC_02460 0.27 -0.1 442,211 - ydaS JDDGAC_02460 0.27 -1.4 442,211 - ydaS JDDGAC_02460 0.27 -0.9 442,242 + ydaS JDDGAC_02460 0.37 -1.3 442,243 - ydaS JDDGAC_02460 0.38 -1.7 442,243 - ydaS JDDGAC_02460 0.38 -0.2 442,292 + ydaS JDDGAC_02460 0.54 +2.7 442,293 - ydaS JDDGAC_02460 0.54 +0.3 442,329 + ydaS JDDGAC_02460 0.66 -1.5 442,330 - ydaS JDDGAC_02460 0.66 -1.3 442,376 + ydaS JDDGAC_02460 0.82 +0.5 442,398 + ydaS JDDGAC_02460 0.89 -1.6 442,398 + ydaS JDDGAC_02460 0.89 -0.3
Or see this region's nucleotide sequence