Experiment: R2A with 3-octanone 15.9309 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS15115 and ABIE40_RS15120 overlap by 4 nucleotides ABIE40_RS15120 and ABIE40_RS15125 are separated by 35 nucleotides ABIE40_RS15125 and ABIE40_RS15130 are separated by 34 nucleotides
ABIE40_RS15115: ABIE40_RS15115 - ABC transporter permease subunit, at 2,970,544 to 2,971,455
_RS15115
ABIE40_RS15120: ABIE40_RS15120 - ABC transporter permease subunit, at 2,971,452 to 2,972,366
_RS15120
ABIE40_RS15125: ABIE40_RS15125 - sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC, at 2,972,402 to 2,973,481
_RS15125
ABIE40_RS15130: ABIE40_RS15130 - phosphodiesterase, at 2,973,516 to 2,974,367
_RS15130
Position (kb)
2972
2973
2974 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2971.420 kb on + strand at 2971.533 kb on + strand at 2971.533 kb on + strand at 2971.533 kb on + strand at 2971.533 kb on + strand at 2971.533 kb on + strand at 2971.533 kb on + strand at 2971.533 kb on + strand at 2971.533 kb on + strand at 2971.533 kb on + strand at 2971.533 kb on + strand at 2971.534 kb on - strand at 2971.534 kb on - strand at 2971.534 kb on - strand at 2971.534 kb on - strand at 2971.534 kb on - strand at 2971.534 kb on - strand at 2971.534 kb on - strand at 2971.534 kb on - strand at 2971.662 kb on + strand, within ABIE40_RS15120 at 2971.662 kb on + strand, within ABIE40_RS15120 at 2971.662 kb on + strand, within ABIE40_RS15120 at 2971.662 kb on + strand, within ABIE40_RS15120 at 2971.663 kb on - strand, within ABIE40_RS15120 at 2971.663 kb on - strand, within ABIE40_RS15120 at 2971.663 kb on - strand, within ABIE40_RS15120 at 2971.663 kb on - strand, within ABIE40_RS15120 at 2971.663 kb on - strand, within ABIE40_RS15120 at 2971.663 kb on - strand, within ABIE40_RS15120 at 2971.663 kb on - strand, within ABIE40_RS15120 at 2971.663 kb on - strand, within ABIE40_RS15120 at 2971.663 kb on - strand, within ABIE40_RS15120 at 2971.663 kb on - strand, within ABIE40_RS15120 at 2971.663 kb on - strand, within ABIE40_RS15120 at 2971.664 kb on + strand, within ABIE40_RS15120 at 2971.664 kb on + strand, within ABIE40_RS15120 at 2971.664 kb on + strand, within ABIE40_RS15120 at 2971.664 kb on + strand, within ABIE40_RS15120 at 2971.665 kb on - strand, within ABIE40_RS15120 at 2971.665 kb on - strand, within ABIE40_RS15120 at 2971.665 kb on - strand, within ABIE40_RS15120 at 2971.665 kb on - strand, within ABIE40_RS15120 at 2971.665 kb on - strand, within ABIE40_RS15120 at 2971.665 kb on - strand, within ABIE40_RS15120 at 2971.743 kb on + strand, within ABIE40_RS15120 at 2971.743 kb on + strand, within ABIE40_RS15120 at 2971.744 kb on - strand, within ABIE40_RS15120 at 2971.830 kb on + strand, within ABIE40_RS15120 at 2971.830 kb on + strand, within ABIE40_RS15120 at 2971.830 kb on + strand, within ABIE40_RS15120 at 2971.831 kb on - strand, within ABIE40_RS15120 at 2971.831 kb on - strand, within ABIE40_RS15120 at 2971.831 kb on - strand, within ABIE40_RS15120 at 2971.831 kb on - strand, within ABIE40_RS15120 at 2972.163 kb on + strand, within ABIE40_RS15120 at 2972.163 kb on + strand, within ABIE40_RS15120 at 2972.163 kb on + strand, within ABIE40_RS15120 at 2972.163 kb on + strand, within ABIE40_RS15120 at 2972.163 kb on + strand, within ABIE40_RS15120 at 2972.164 kb on - strand, within ABIE40_RS15120 at 2972.164 kb on - strand, within ABIE40_RS15120 at 2972.164 kb on - strand, within ABIE40_RS15120 at 2972.164 kb on - strand, within ABIE40_RS15120 at 2972.164 kb on - strand, within ABIE40_RS15120 at 2972.199 kb on + strand, within ABIE40_RS15120 at 2972.382 kb on + strand at 2972.383 kb on - strand at 2972.394 kb on + strand at 2972.395 kb on - strand at 2972.395 kb on - strand at 2972.651 kb on + strand, within ABIE40_RS15125 at 2972.651 kb on + strand, within ABIE40_RS15125 at 2972.652 kb on - strand, within ABIE40_RS15125 at 2972.660 kb on + strand, within ABIE40_RS15125 at 2972.668 kb on + strand, within ABIE40_RS15125 at 2972.668 kb on + strand, within ABIE40_RS15125 at 2972.668 kb on + strand, within ABIE40_RS15125 at 2972.668 kb on + strand, within ABIE40_RS15125 at 2972.668 kb on + strand, within ABIE40_RS15125 at 2972.668 kb on + strand, within ABIE40_RS15125 at 2972.668 kb on + strand, within ABIE40_RS15125 at 2972.668 kb on + strand, within ABIE40_RS15125 at 2972.668 kb on + strand, within ABIE40_RS15125 at 2972.777 kb on + strand, within ABIE40_RS15125 at 2972.777 kb on + strand, within ABIE40_RS15125 at 2972.777 kb on + strand, within ABIE40_RS15125 at 2972.778 kb on - strand, within ABIE40_RS15125 at 2973.062 kb on - strand, within ABIE40_RS15125 at 2973.065 kb on + strand, within ABIE40_RS15125 at 2973.065 kb on + strand, within ABIE40_RS15125 at 2973.065 kb on + strand, within ABIE40_RS15125 at 2973.065 kb on + strand, within ABIE40_RS15125 at 2973.065 kb on + strand, within ABIE40_RS15125 at 2973.066 kb on - strand, within ABIE40_RS15125 at 2973.066 kb on - strand, within ABIE40_RS15125 at 2973.066 kb on - strand, within ABIE40_RS15125 at 2973.066 kb on - strand, within ABIE40_RS15125 at 2973.066 kb on - strand, within ABIE40_RS15125 at 2973.066 kb on - strand, within ABIE40_RS15125 at 2973.066 kb on - strand, within ABIE40_RS15125 at 2973.066 kb on - strand, within ABIE40_RS15125 at 2973.066 kb on - strand, within ABIE40_RS15125 at 2973.066 kb on - strand, within ABIE40_RS15125 at 2973.068 kb on - strand, within ABIE40_RS15125 at 2973.159 kb on + strand, within ABIE40_RS15125 at 2973.160 kb on - strand, within ABIE40_RS15125 at 2973.283 kb on - strand, within ABIE40_RS15125 at 2973.453 kb on - strand at 2973.487 kb on + strand at 2973.503 kb on + strand at 2973.503 kb on + strand at 2973.574 kb on + strand at 2973.578 kb on + strand at 2973.694 kb on - strand, within ABIE40_RS15130 at 2973.710 kb on + strand, within ABIE40_RS15130 at 2973.711 kb on - strand, within ABIE40_RS15130 at 2973.746 kb on + strand, within ABIE40_RS15130 at 2973.747 kb on - strand, within ABIE40_RS15130 at 2973.774 kb on - strand, within ABIE40_RS15130 at 2973.840 kb on + strand, within ABIE40_RS15130 at 2973.841 kb on - strand, within ABIE40_RS15130 at 2973.841 kb on - strand, within ABIE40_RS15130 at 2973.841 kb on - strand, within ABIE40_RS15130 at 2973.841 kb on - strand, within ABIE40_RS15130 at 2973.890 kb on + strand, within ABIE40_RS15130 at 2973.900 kb on - strand, within ABIE40_RS15130 at 2973.963 kb on + strand, within ABIE40_RS15130 at 2974.080 kb on + strand, within ABIE40_RS15130 at 2974.080 kb on + strand, within ABIE40_RS15130 at 2974.080 kb on + strand, within ABIE40_RS15130 at 2974.080 kb on + strand, within ABIE40_RS15130 at 2974.080 kb on + strand, within ABIE40_RS15130 at 2974.081 kb on - strand, within ABIE40_RS15130 at 2974.144 kb on - strand, within ABIE40_RS15130 at 2974.215 kb on + strand, within ABIE40_RS15130 at 2974.215 kb on + strand, within ABIE40_RS15130 at 2974.215 kb on + strand, within ABIE40_RS15130 at 2974.216 kb on - strand, within ABIE40_RS15130 at 2974.216 kb on - strand, within ABIE40_RS15130 at 2974.216 kb on - strand, within ABIE40_RS15130 at 2974.272 kb on + strand, within ABIE40_RS15130 at 2974.343 kb on + strand at 2974.343 kb on + strand at 2974.343 kb on + strand at 2974.343 kb on + strand at 2974.344 kb on - strand at 2974.344 kb on - strand at 2974.442 kb on - strand at 2974.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 15.9309 mM remove 2,971,420 + -0.4 2,971,533 + -0.1 2,971,533 + -1.7 2,971,533 + -0.1 2,971,533 + -0.6 2,971,533 + -1.4 2,971,533 + +0.7 2,971,533 + +2.4 2,971,533 + -1.8 2,971,533 + -0.7 2,971,533 + +1.8 2,971,534 - -0.2 2,971,534 - -0.1 2,971,534 - -0.7 2,971,534 - -0.6 2,971,534 - +0.8 2,971,534 - -0.9 2,971,534 - -1.6 2,971,534 - +1.5 2,971,662 + ABIE40_RS15120 0.23 -1.4 2,971,662 + ABIE40_RS15120 0.23 -0.2 2,971,662 + ABIE40_RS15120 0.23 -0.2 2,971,662 + ABIE40_RS15120 0.23 -0.4 2,971,663 - ABIE40_RS15120 0.23 +0.3 2,971,663 - ABIE40_RS15120 0.23 +0.6 2,971,663 - ABIE40_RS15120 0.23 -1.4 2,971,663 - ABIE40_RS15120 0.23 -1.5 2,971,663 - ABIE40_RS15120 0.23 -0.6 2,971,663 - ABIE40_RS15120 0.23 -0.8 2,971,663 - ABIE40_RS15120 0.23 -0.6 2,971,663 - ABIE40_RS15120 0.23 +0.1 2,971,663 - ABIE40_RS15120 0.23 -0.3 2,971,663 - ABIE40_RS15120 0.23 -1.1 2,971,663 - ABIE40_RS15120 0.23 +0.0 2,971,664 + ABIE40_RS15120 0.23 -1.1 2,971,664 + ABIE40_RS15120 0.23 -1.4 2,971,664 + ABIE40_RS15120 0.23 -2.6 2,971,664 + ABIE40_RS15120 0.23 -1.7 2,971,665 - ABIE40_RS15120 0.23 -0.4 2,971,665 - ABIE40_RS15120 0.23 +0.8 2,971,665 - ABIE40_RS15120 0.23 -0.1 2,971,665 - ABIE40_RS15120 0.23 -0.0 2,971,665 - ABIE40_RS15120 0.23 +0.3 2,971,665 - ABIE40_RS15120 0.23 -0.6 2,971,743 + ABIE40_RS15120 0.32 -0.6 2,971,743 + ABIE40_RS15120 0.32 +0.0 2,971,744 - ABIE40_RS15120 0.32 +1.1 2,971,830 + ABIE40_RS15120 0.41 +0.0 2,971,830 + ABIE40_RS15120 0.41 +0.9 2,971,830 + ABIE40_RS15120 0.41 +3.2 2,971,831 - ABIE40_RS15120 0.41 -1.0 2,971,831 - ABIE40_RS15120 0.41 +1.5 2,971,831 - ABIE40_RS15120 0.41 -1.1 2,971,831 - ABIE40_RS15120 0.41 -2.0 2,972,163 + ABIE40_RS15120 0.78 -1.9 2,972,163 + ABIE40_RS15120 0.78 -0.3 2,972,163 + ABIE40_RS15120 0.78 +2.8 2,972,163 + ABIE40_RS15120 0.78 +2.1 2,972,163 + ABIE40_RS15120 0.78 +0.2 2,972,164 - ABIE40_RS15120 0.78 -1.4 2,972,164 - ABIE40_RS15120 0.78 +2.6 2,972,164 - ABIE40_RS15120 0.78 -0.6 2,972,164 - ABIE40_RS15120 0.78 -2.5 2,972,164 - ABIE40_RS15120 0.78 -0.4 2,972,199 + ABIE40_RS15120 0.82 +0.1 2,972,382 + -1.2 2,972,383 - -0.6 2,972,394 + -1.8 2,972,395 - +0.2 2,972,395 - +0.6 2,972,651 + ABIE40_RS15125 0.23 -0.1 2,972,651 + ABIE40_RS15125 0.23 +0.4 2,972,652 - ABIE40_RS15125 0.23 +1.8 2,972,660 + ABIE40_RS15125 0.24 -1.9 2,972,668 + ABIE40_RS15125 0.25 +0.4 2,972,668 + ABIE40_RS15125 0.25 +1.4 2,972,668 + ABIE40_RS15125 0.25 +0.7 2,972,668 + ABIE40_RS15125 0.25 -0.4 2,972,668 + ABIE40_RS15125 0.25 -1.9 2,972,668 + ABIE40_RS15125 0.25 +1.2 2,972,668 + ABIE40_RS15125 0.25 +0.5 2,972,668 + ABIE40_RS15125 0.25 -0.2 2,972,668 + ABIE40_RS15125 0.25 +0.7 2,972,777 + ABIE40_RS15125 0.35 +0.6 2,972,777 + ABIE40_RS15125 0.35 +0.8 2,972,777 + ABIE40_RS15125 0.35 -0.6 2,972,778 - ABIE40_RS15125 0.35 +0.3 2,973,062 - ABIE40_RS15125 0.61 +0.2 2,973,065 + ABIE40_RS15125 0.61 -0.9 2,973,065 + ABIE40_RS15125 0.61 -0.2 2,973,065 + ABIE40_RS15125 0.61 +0.5 2,973,065 + ABIE40_RS15125 0.61 +1.4 2,973,065 + ABIE40_RS15125 0.61 +1.9 2,973,066 - ABIE40_RS15125 0.61 -0.4 2,973,066 - ABIE40_RS15125 0.61 +0.2 2,973,066 - ABIE40_RS15125 0.61 +1.4 2,973,066 - ABIE40_RS15125 0.61 +1.3 2,973,066 - ABIE40_RS15125 0.61 -0.7 2,973,066 - ABIE40_RS15125 0.61 +0.3 2,973,066 - ABIE40_RS15125 0.61 -0.7 2,973,066 - ABIE40_RS15125 0.61 -0.3 2,973,066 - ABIE40_RS15125 0.61 -2.6 2,973,066 - ABIE40_RS15125 0.61 +0.2 2,973,068 - ABIE40_RS15125 0.62 +0.4 2,973,159 + ABIE40_RS15125 0.70 +3.8 2,973,160 - ABIE40_RS15125 0.70 -1.4 2,973,283 - ABIE40_RS15125 0.82 +0.5 2,973,453 - +0.2 2,973,487 + -0.9 2,973,503 + +1.2 2,973,503 + -0.7 2,973,574 + -1.0 2,973,578 + -1.2 2,973,694 - ABIE40_RS15130 0.21 -0.9 2,973,710 + ABIE40_RS15130 0.23 -0.5 2,973,711 - ABIE40_RS15130 0.23 -1.7 2,973,746 + ABIE40_RS15130 0.27 -1.0 2,973,747 - ABIE40_RS15130 0.27 -0.6 2,973,774 - ABIE40_RS15130 0.30 +0.2 2,973,840 + ABIE40_RS15130 0.38 -3.9 2,973,841 - ABIE40_RS15130 0.38 -2.6 2,973,841 - ABIE40_RS15130 0.38 -0.1 2,973,841 - ABIE40_RS15130 0.38 -1.7 2,973,841 - ABIE40_RS15130 0.38 -0.8 2,973,890 + ABIE40_RS15130 0.44 -2.5 2,973,900 - ABIE40_RS15130 0.45 +0.6 2,973,963 + ABIE40_RS15130 0.52 +0.4 2,974,080 + ABIE40_RS15130 0.66 -0.4 2,974,080 + ABIE40_RS15130 0.66 -0.6 2,974,080 + ABIE40_RS15130 0.66 +0.3 2,974,080 + ABIE40_RS15130 0.66 -1.0 2,974,080 + ABIE40_RS15130 0.66 -1.0 2,974,081 - ABIE40_RS15130 0.66 +0.8 2,974,144 - ABIE40_RS15130 0.74 +1.0 2,974,215 + ABIE40_RS15130 0.82 +0.2 2,974,215 + ABIE40_RS15130 0.82 -0.2 2,974,215 + ABIE40_RS15130 0.82 -1.1 2,974,216 - ABIE40_RS15130 0.82 +0.4 2,974,216 - ABIE40_RS15130 0.82 -0.4 2,974,216 - ABIE40_RS15130 0.82 -0.1 2,974,272 + ABIE40_RS15130 0.89 -0.0 2,974,343 + +1.0 2,974,343 + -0.5 2,974,343 + -0.7 2,974,343 + -0.7 2,974,344 - -0.1 2,974,344 - -0.1 2,974,442 - +0.6 2,974,468 - -0.0
Or see this region's nucleotide sequence