Experiment: R2A with 3-octanone 15.9309 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS03120 and ABIE40_RS03125 are separated by 35 nucleotides ABIE40_RS03125 and ABIE40_RS03130 are separated by 12 nucleotides ABIE40_RS03130 and ABIE40_RS03135 overlap by 1 nucleotides ABIE40_RS03135 and ABIE40_RS03140 are separated by 290 nucleotides
ABIE40_RS03120: ABIE40_RS03120 - hypothetical protein, at 615,280 to 615,690
_RS03120
ABIE40_RS03125: ABIE40_RS03125 - rod-binding protein, at 615,726 to 616,286
_RS03125
ABIE40_RS03130: ABIE40_RS03130 - hypothetical protein, at 616,299 to 616,664
_RS03130
ABIE40_RS03135: ABIE40_RS03135 - hypothetical protein, at 616,664 to 617,206
_RS03135
ABIE40_RS03140: ABIE40_RS03140 - WecB/TagA/CpsF family glycosyltransferase, at 617,497 to 618,336
_RS03140
Position (kb)
616
617 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 615.355 kb on + strand, within ABIE40_RS03120 at 615.356 kb on + strand, within ABIE40_RS03120 at 615.356 kb on - strand, within ABIE40_RS03120 at 615.356 kb on - strand, within ABIE40_RS03120 at 615.356 kb on - strand, within ABIE40_RS03120 at 615.356 kb on - strand, within ABIE40_RS03120 at 615.356 kb on - strand, within ABIE40_RS03120 at 615.466 kb on + strand, within ABIE40_RS03120 at 615.466 kb on + strand, within ABIE40_RS03120 at 615.466 kb on + strand, within ABIE40_RS03120 at 615.466 kb on + strand, within ABIE40_RS03120 at 615.466 kb on + strand, within ABIE40_RS03120 at 615.466 kb on + strand, within ABIE40_RS03120 at 615.467 kb on - strand, within ABIE40_RS03120 at 615.467 kb on - strand, within ABIE40_RS03120 at 615.467 kb on - strand, within ABIE40_RS03120 at 615.467 kb on - strand, within ABIE40_RS03120 at 615.467 kb on - strand, within ABIE40_RS03120 at 615.467 kb on - strand, within ABIE40_RS03120 at 615.467 kb on - strand, within ABIE40_RS03120 at 615.634 kb on + strand, within ABIE40_RS03120 at 615.634 kb on + strand, within ABIE40_RS03120 at 615.635 kb on - strand, within ABIE40_RS03120 at 615.635 kb on - strand, within ABIE40_RS03120 at 615.639 kb on + strand, within ABIE40_RS03120 at 615.706 kb on + strand at 615.706 kb on + strand at 615.706 kb on + strand at 615.706 kb on + strand at 615.706 kb on + strand at 615.707 kb on - strand at 615.707 kb on - strand at 615.707 kb on - strand at 615.707 kb on - strand at 615.711 kb on + strand at 615.711 kb on + strand at 615.711 kb on + strand at 615.711 kb on + strand at 615.731 kb on + strand at 615.731 kb on + strand at 616.135 kb on - strand, within ABIE40_RS03125 at 616.135 kb on - strand, within ABIE40_RS03125 at 616.135 kb on - strand, within ABIE40_RS03125 at 616.275 kb on - strand at 616.275 kb on - strand at 616.321 kb on - strand at 616.325 kb on + strand at 616.326 kb on - strand at 616.373 kb on + strand, within ABIE40_RS03130 at 616.373 kb on + strand, within ABIE40_RS03130 at 616.373 kb on + strand, within ABIE40_RS03130 at 616.374 kb on - strand, within ABIE40_RS03130 at 616.374 kb on - strand, within ABIE40_RS03130 at 616.374 kb on - strand, within ABIE40_RS03130 at 616.374 kb on - strand, within ABIE40_RS03130 at 616.374 kb on - strand, within ABIE40_RS03130 at 616.374 kb on - strand, within ABIE40_RS03130 at 616.374 kb on - strand, within ABIE40_RS03130 at 616.374 kb on - strand, within ABIE40_RS03130 at 616.380 kb on - strand, within ABIE40_RS03130 at 616.412 kb on - strand, within ABIE40_RS03130 at 616.440 kb on + strand, within ABIE40_RS03130 at 616.440 kb on + strand, within ABIE40_RS03130 at 616.440 kb on + strand, within ABIE40_RS03130 at 616.440 kb on + strand, within ABIE40_RS03130 at 616.440 kb on + strand, within ABIE40_RS03130 at 616.441 kb on - strand, within ABIE40_RS03130 at 616.441 kb on - strand, within ABIE40_RS03130 at 616.441 kb on - strand, within ABIE40_RS03130 at 616.625 kb on + strand, within ABIE40_RS03130 at 616.625 kb on + strand, within ABIE40_RS03130 at 616.626 kb on - strand, within ABIE40_RS03130 at 616.626 kb on - strand, within ABIE40_RS03130 at 616.626 kb on - strand, within ABIE40_RS03130 at 616.629 kb on + strand at 616.629 kb on + strand at 616.629 kb on + strand at 616.630 kb on - strand at 616.630 kb on - strand at 616.630 kb on - strand at 616.630 kb on - strand at 616.658 kb on + strand at 616.658 kb on + strand at 616.658 kb on + strand at 616.659 kb on - strand at 616.669 kb on + strand at 616.669 kb on + strand at 616.669 kb on + strand at 616.669 kb on + strand at 616.669 kb on + strand at 616.670 kb on - strand at 616.681 kb on + strand at 616.721 kb on + strand, within ABIE40_RS03135 at 616.721 kb on + strand, within ABIE40_RS03135 at 616.721 kb on + strand, within ABIE40_RS03135 at 616.722 kb on - strand, within ABIE40_RS03135 at 616.969 kb on + strand, within ABIE40_RS03135 at 616.970 kb on - strand, within ABIE40_RS03135 at 617.051 kb on + strand, within ABIE40_RS03135 at 617.051 kb on + strand, within ABIE40_RS03135 at 617.051 kb on + strand, within ABIE40_RS03135 at 617.051 kb on + strand, within ABIE40_RS03135 at 617.052 kb on - strand, within ABIE40_RS03135 at 617.052 kb on - strand, within ABIE40_RS03135 at 617.052 kb on - strand, within ABIE40_RS03135 at 617.052 kb on - strand, within ABIE40_RS03135 at 617.052 kb on - strand, within ABIE40_RS03135 at 617.054 kb on - strand, within ABIE40_RS03135 at 617.084 kb on + strand, within ABIE40_RS03135 at 617.084 kb on + strand, within ABIE40_RS03135 at 617.084 kb on + strand, within ABIE40_RS03135 at 617.084 kb on + strand, within ABIE40_RS03135 at 617.085 kb on - strand, within ABIE40_RS03135 at 617.085 kb on - strand, within ABIE40_RS03135 at 617.085 kb on - strand, within ABIE40_RS03135 at 617.085 kb on - strand, within ABIE40_RS03135 at 617.085 kb on - strand, within ABIE40_RS03135 at 617.085 kb on - strand, within ABIE40_RS03135 at 617.210 kb on + strand at 617.211 kb on - strand at 617.343 kb on + strand at 617.343 kb on + strand at 617.343 kb on + strand at 617.344 kb on - strand at 617.344 kb on - strand at 617.344 kb on - strand at 617.344 kb on - strand at 617.345 kb on + strand at 617.346 kb on - strand at 617.346 kb on - strand at 617.346 kb on - strand at 617.523 kb on + strand at 617.523 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 15.9309 mM remove 615,355 + ABIE40_RS03120 0.18 -2.0 615,356 + ABIE40_RS03120 0.18 -1.5 615,356 - ABIE40_RS03120 0.18 -2.2 615,356 - ABIE40_RS03120 0.18 -1.4 615,356 - ABIE40_RS03120 0.18 +1.0 615,356 - ABIE40_RS03120 0.18 -0.8 615,356 - ABIE40_RS03120 0.18 -3.6 615,466 + ABIE40_RS03120 0.45 -1.6 615,466 + ABIE40_RS03120 0.45 -2.8 615,466 + ABIE40_RS03120 0.45 +0.1 615,466 + ABIE40_RS03120 0.45 -1.8 615,466 + ABIE40_RS03120 0.45 -1.0 615,466 + ABIE40_RS03120 0.45 -2.0 615,467 - ABIE40_RS03120 0.45 -1.3 615,467 - ABIE40_RS03120 0.45 -0.2 615,467 - ABIE40_RS03120 0.45 -0.6 615,467 - ABIE40_RS03120 0.45 -2.6 615,467 - ABIE40_RS03120 0.45 -0.2 615,467 - ABIE40_RS03120 0.45 -0.7 615,467 - ABIE40_RS03120 0.45 -1.2 615,634 + ABIE40_RS03120 0.86 -1.6 615,634 + ABIE40_RS03120 0.86 -2.4 615,635 - ABIE40_RS03120 0.86 -1.4 615,635 - ABIE40_RS03120 0.86 -0.5 615,639 + ABIE40_RS03120 0.87 -2.4 615,706 + +0.7 615,706 + +0.8 615,706 + -2.7 615,706 + -0.5 615,706 + -1.4 615,707 - -2.7 615,707 - +0.8 615,707 - -1.2 615,707 - +0.2 615,711 + -0.4 615,711 + -1.8 615,711 + -2.8 615,711 + -0.5 615,731 + -2.2 615,731 + -1.0 616,135 - ABIE40_RS03125 0.73 -1.8 616,135 - ABIE40_RS03125 0.73 -2.6 616,135 - ABIE40_RS03125 0.73 -2.8 616,275 - -0.8 616,275 - +0.6 616,321 - -1.2 616,325 + -0.5 616,326 - -2.0 616,373 + ABIE40_RS03130 0.20 -1.7 616,373 + ABIE40_RS03130 0.20 -1.6 616,373 + ABIE40_RS03130 0.20 -1.5 616,374 - ABIE40_RS03130 0.20 -1.7 616,374 - ABIE40_RS03130 0.20 -3.1 616,374 - ABIE40_RS03130 0.20 +0.6 616,374 - ABIE40_RS03130 0.20 -1.3 616,374 - ABIE40_RS03130 0.20 -0.7 616,374 - ABIE40_RS03130 0.20 -0.2 616,374 - ABIE40_RS03130 0.20 -1.5 616,374 - ABIE40_RS03130 0.20 -1.4 616,380 - ABIE40_RS03130 0.22 -0.5 616,412 - ABIE40_RS03130 0.31 -0.8 616,440 + ABIE40_RS03130 0.39 -1.3 616,440 + ABIE40_RS03130 0.39 +0.2 616,440 + ABIE40_RS03130 0.39 -1.3 616,440 + ABIE40_RS03130 0.39 -0.4 616,440 + ABIE40_RS03130 0.39 -0.9 616,441 - ABIE40_RS03130 0.39 -0.0 616,441 - ABIE40_RS03130 0.39 +0.2 616,441 - ABIE40_RS03130 0.39 -2.2 616,625 + ABIE40_RS03130 0.89 +1.2 616,625 + ABIE40_RS03130 0.89 -1.2 616,626 - ABIE40_RS03130 0.89 +0.1 616,626 - ABIE40_RS03130 0.89 -2.6 616,626 - ABIE40_RS03130 0.89 -1.2 616,629 + -0.7 616,629 + -0.8 616,629 + -1.9 616,630 - -1.4 616,630 - -1.5 616,630 - -0.2 616,630 - -0.5 616,658 + -2.6 616,658 + +0.5 616,658 + -0.7 616,659 - -2.6 616,669 + -1.9 616,669 + +0.6 616,669 + +0.4 616,669 + -0.8 616,669 + -0.6 616,670 - -0.5 616,681 + +0.1 616,721 + ABIE40_RS03135 0.10 -0.1 616,721 + ABIE40_RS03135 0.10 +0.8 616,721 + ABIE40_RS03135 0.10 +1.0 616,722 - ABIE40_RS03135 0.11 -1.0 616,969 + ABIE40_RS03135 0.56 +0.4 616,970 - ABIE40_RS03135 0.56 +1.2 617,051 + ABIE40_RS03135 0.71 +0.3 617,051 + ABIE40_RS03135 0.71 +1.2 617,051 + ABIE40_RS03135 0.71 +0.3 617,051 + ABIE40_RS03135 0.71 +0.4 617,052 - ABIE40_RS03135 0.71 +0.2 617,052 - ABIE40_RS03135 0.71 +0.8 617,052 - ABIE40_RS03135 0.71 -2.5 617,052 - ABIE40_RS03135 0.71 +0.1 617,052 - ABIE40_RS03135 0.71 -0.3 617,054 - ABIE40_RS03135 0.72 -1.0 617,084 + ABIE40_RS03135 0.77 +0.8 617,084 + ABIE40_RS03135 0.77 +0.5 617,084 + ABIE40_RS03135 0.77 +0.0 617,084 + ABIE40_RS03135 0.77 -0.0 617,085 - ABIE40_RS03135 0.78 +0.3 617,085 - ABIE40_RS03135 0.78 +0.7 617,085 - ABIE40_RS03135 0.78 -0.1 617,085 - ABIE40_RS03135 0.78 +1.4 617,085 - ABIE40_RS03135 0.78 -1.6 617,085 - ABIE40_RS03135 0.78 -0.8 617,210 + +0.7 617,211 - -0.5 617,343 + -0.7 617,343 + -3.4 617,343 + -0.8 617,344 - +1.8 617,344 - +0.4 617,344 - +1.1 617,344 - -0.5 617,345 + +1.8 617,346 - +0.9 617,346 - +2.0 617,346 - -0.1 617,523 + -0.1 617,523 + -0.4
Or see this region's nucleotide sequence