Experiment: R2A with 3-octanone 15.9309 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS20635 and ABIE40_RS20640 are separated by 4 nucleotides ABIE40_RS20640 and ABIE40_RS20645 overlap by 4 nucleotides ABIE40_RS20645 and ABIE40_RS20650 are separated by 196 nucleotides
ABIE40_RS20635: ABIE40_RS20635 - 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD, at 233,957 to 234,712
_RS20635
ABIE40_RS20640: ABIE40_RS20640 - 5-dehydro-4-deoxy-D-glucuronate isomerase, at 234,717 to 235,553
_RS20640
ABIE40_RS20645: ABIE40_RS20645 - GntR family transcriptional regulator, at 235,550 to 236,320
_RS20645
ABIE40_RS20650: ABIE40_RS20650 - DUF5054 domain-containing protein, at 236,517 to 238,430
_RS20650
Position (kb) 235
236
237 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 234.718 kb on + strand at 234.719 kb on - strand at 234.719 kb on - strand at 234.719 kb on - strand at 234.719 kb on - strand at 234.796 kb on + strand at 234.796 kb on + strand at 234.796 kb on + strand at 234.796 kb on + strand at 234.797 kb on - strand at 234.797 kb on - strand at 234.797 kb on - strand at 234.797 kb on - strand at 234.934 kb on + strand, within ABIE40_RS20640 at 234.935 kb on - strand, within ABIE40_RS20640 at 234.935 kb on - strand, within ABIE40_RS20640 at 234.935 kb on - strand, within ABIE40_RS20640 at 234.940 kb on + strand, within ABIE40_RS20640 at 234.940 kb on + strand, within ABIE40_RS20640 at 234.940 kb on + strand, within ABIE40_RS20640 at 234.941 kb on - strand, within ABIE40_RS20640 at 234.941 kb on - strand, within ABIE40_RS20640 at 234.941 kb on - strand, within ABIE40_RS20640 at 234.965 kb on + strand, within ABIE40_RS20640 at 234.965 kb on + strand, within ABIE40_RS20640 at 235.063 kb on + strand, within ABIE40_RS20640 at 235.063 kb on + strand, within ABIE40_RS20640 at 235.064 kb on - strand, within ABIE40_RS20640 at 235.067 kb on + strand, within ABIE40_RS20640 at 235.067 kb on + strand, within ABIE40_RS20640 at 235.067 kb on + strand, within ABIE40_RS20640 at 235.067 kb on + strand, within ABIE40_RS20640 at 235.067 kb on + strand, within ABIE40_RS20640 at 235.068 kb on - strand, within ABIE40_RS20640 at 235.068 kb on - strand, within ABIE40_RS20640 at 235.068 kb on - strand, within ABIE40_RS20640 at 235.068 kb on - strand, within ABIE40_RS20640 at 235.174 kb on + strand, within ABIE40_RS20640 at 235.175 kb on - strand, within ABIE40_RS20640 at 235.240 kb on + strand, within ABIE40_RS20640 at 235.240 kb on + strand, within ABIE40_RS20640 at 235.241 kb on - strand, within ABIE40_RS20640 at 235.241 kb on - strand, within ABIE40_RS20640 at 235.241 kb on - strand, within ABIE40_RS20640 at 235.241 kb on - strand, within ABIE40_RS20640 at 235.252 kb on + strand, within ABIE40_RS20640 at 235.252 kb on + strand, within ABIE40_RS20640 at 235.267 kb on + strand, within ABIE40_RS20640 at 235.267 kb on + strand, within ABIE40_RS20640 at 235.268 kb on - strand, within ABIE40_RS20640 at 235.432 kb on + strand, within ABIE40_RS20640 at 235.432 kb on + strand, within ABIE40_RS20640 at 235.505 kb on - strand at 235.544 kb on - strand at 235.611 kb on + strand at 235.611 kb on + strand at 235.846 kb on + strand, within ABIE40_RS20645 at 235.847 kb on - strand, within ABIE40_RS20645 at 235.997 kb on - strand, within ABIE40_RS20645 at 235.997 kb on - strand, within ABIE40_RS20645 at 236.229 kb on + strand, within ABIE40_RS20645 at 236.230 kb on - strand, within ABIE40_RS20645 at 236.349 kb on + strand at 236.349 kb on + strand at 236.408 kb on + strand at 236.501 kb on - strand at 236.684 kb on + strand at 236.880 kb on + strand, within ABIE40_RS20650 at 237.100 kb on + strand, within ABIE40_RS20650 at 237.100 kb on + strand, within ABIE40_RS20650 at 237.100 kb on + strand, within ABIE40_RS20650 at 237.100 kb on + strand, within ABIE40_RS20650 at 237.100 kb on + strand, within ABIE40_RS20650 at 237.100 kb on + strand, within ABIE40_RS20650 at 237.100 kb on + strand, within ABIE40_RS20650 at 237.100 kb on + strand, within ABIE40_RS20650 at 237.101 kb on - strand, within ABIE40_RS20650 at 237.101 kb on - strand, within ABIE40_RS20650 at 237.101 kb on - strand, within ABIE40_RS20650 at 237.110 kb on + strand, within ABIE40_RS20650 at 237.110 kb on + strand, within ABIE40_RS20650 at 237.110 kb on + strand, within ABIE40_RS20650 at 237.110 kb on + strand, within ABIE40_RS20650 at 237.110 kb on + strand, within ABIE40_RS20650 at 237.111 kb on - strand, within ABIE40_RS20650 at 237.111 kb on - strand, within ABIE40_RS20650 at 237.111 kb on - strand, within ABIE40_RS20650 at 237.111 kb on - strand, within ABIE40_RS20650 at 237.111 kb on - strand, within ABIE40_RS20650 at 237.136 kb on + strand, within ABIE40_RS20650 at 237.137 kb on - strand, within ABIE40_RS20650 at 237.137 kb on - strand, within ABIE40_RS20650 at 237.137 kb on - strand, within ABIE40_RS20650 at 237.137 kb on - strand, within ABIE40_RS20650 at 237.137 kb on - strand, within ABIE40_RS20650 at 237.137 kb on - strand, within ABIE40_RS20650 at 237.152 kb on - strand, within ABIE40_RS20650 at 237.215 kb on + strand, within ABIE40_RS20650
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 15.9309 mM remove 234,718 + +0.6 234,719 - -1.0 234,719 - +0.1 234,719 - -1.4 234,719 - +3.1 234,796 + -0.1 234,796 + -1.7 234,796 + +1.5 234,796 + -0.9 234,797 - +0.5 234,797 - +1.4 234,797 - +0.5 234,797 - +2.2 234,934 + ABIE40_RS20640 0.26 +0.6 234,935 - ABIE40_RS20640 0.26 +0.0 234,935 - ABIE40_RS20640 0.26 -1.1 234,935 - ABIE40_RS20640 0.26 -0.7 234,940 + ABIE40_RS20640 0.27 -0.2 234,940 + ABIE40_RS20640 0.27 +0.5 234,940 + ABIE40_RS20640 0.27 -0.8 234,941 - ABIE40_RS20640 0.27 -0.1 234,941 - ABIE40_RS20640 0.27 -0.7 234,941 - ABIE40_RS20640 0.27 -0.9 234,965 + ABIE40_RS20640 0.30 +1.0 234,965 + ABIE40_RS20640 0.30 +1.1 235,063 + ABIE40_RS20640 0.41 -0.8 235,063 + ABIE40_RS20640 0.41 -0.3 235,064 - ABIE40_RS20640 0.41 -2.2 235,067 + ABIE40_RS20640 0.42 -0.1 235,067 + ABIE40_RS20640 0.42 -2.1 235,067 + ABIE40_RS20640 0.42 -0.2 235,067 + ABIE40_RS20640 0.42 -1.1 235,067 + ABIE40_RS20640 0.42 +0.6 235,068 - ABIE40_RS20640 0.42 -0.2 235,068 - ABIE40_RS20640 0.42 -0.2 235,068 - ABIE40_RS20640 0.42 -1.6 235,068 - ABIE40_RS20640 0.42 +0.4 235,174 + ABIE40_RS20640 0.55 +0.5 235,175 - ABIE40_RS20640 0.55 -0.5 235,240 + ABIE40_RS20640 0.62 +3.1 235,240 + ABIE40_RS20640 0.62 -0.5 235,241 - ABIE40_RS20640 0.63 -1.1 235,241 - ABIE40_RS20640 0.63 +1.5 235,241 - ABIE40_RS20640 0.63 +0.1 235,241 - ABIE40_RS20640 0.63 -1.4 235,252 + ABIE40_RS20640 0.64 -2.4 235,252 + ABIE40_RS20640 0.64 -1.1 235,267 + ABIE40_RS20640 0.66 +0.3 235,267 + ABIE40_RS20640 0.66 -3.6 235,268 - ABIE40_RS20640 0.66 +0.1 235,432 + ABIE40_RS20640 0.85 -0.5 235,432 + ABIE40_RS20640 0.85 -0.2 235,505 - -0.2 235,544 - +0.0 235,611 + +1.3 235,611 + -2.7 235,846 + ABIE40_RS20645 0.38 +1.5 235,847 - ABIE40_RS20645 0.39 -0.3 235,997 - ABIE40_RS20645 0.58 +1.6 235,997 - ABIE40_RS20645 0.58 +0.9 236,229 + ABIE40_RS20645 0.88 +2.0 236,230 - ABIE40_RS20645 0.88 -2.7 236,349 + -0.7 236,349 + -2.6 236,408 + -0.4 236,501 - -0.1 236,684 + -0.8 236,880 + ABIE40_RS20650 0.19 -2.3 237,100 + ABIE40_RS20650 0.30 -0.8 237,100 + ABIE40_RS20650 0.30 +2.1 237,100 + ABIE40_RS20650 0.30 +1.4 237,100 + ABIE40_RS20650 0.30 -0.4 237,100 + ABIE40_RS20650 0.30 -2.1 237,100 + ABIE40_RS20650 0.30 -2.7 237,100 + ABIE40_RS20650 0.30 +3.7 237,100 + ABIE40_RS20650 0.30 -1.7 237,101 - ABIE40_RS20650 0.31 -0.4 237,101 - ABIE40_RS20650 0.31 +0.9 237,101 - ABIE40_RS20650 0.31 -0.1 237,110 + ABIE40_RS20650 0.31 +0.8 237,110 + ABIE40_RS20650 0.31 +1.0 237,110 + ABIE40_RS20650 0.31 -0.2 237,110 + ABIE40_RS20650 0.31 +2.1 237,110 + ABIE40_RS20650 0.31 -0.5 237,111 - ABIE40_RS20650 0.31 +0.4 237,111 - ABIE40_RS20650 0.31 -1.4 237,111 - ABIE40_RS20650 0.31 +0.4 237,111 - ABIE40_RS20650 0.31 +1.5 237,111 - ABIE40_RS20650 0.31 -2.6 237,136 + ABIE40_RS20650 0.32 +0.5 237,137 - ABIE40_RS20650 0.32 +0.0 237,137 - ABIE40_RS20650 0.32 -0.1 237,137 - ABIE40_RS20650 0.32 -0.2 237,137 - ABIE40_RS20650 0.32 +0.3 237,137 - ABIE40_RS20650 0.32 -0.1 237,137 - ABIE40_RS20650 0.32 -0.3 237,152 - ABIE40_RS20650 0.33 -2.8 237,215 + ABIE40_RS20650 0.36 +0.2
Or see this region's nucleotide sequence