Experiment: R2A with 3-octanone 15.9309 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS17695 and ABIE40_RS17700 overlap by 4 nucleotides ABIE40_RS17700 and ABIE40_RS17705 are separated by 3 nucleotides
ABIE40_RS17695: ABIE40_RS17695 - murein L,D-transpeptidase family protein, at 3,532,275 to 3,533,534
_RS17695
ABIE40_RS17700: ABIE40_RS17700 - sulfurtransferase TusA family protein, at 3,533,531 to 3,533,764
_RS17700
ABIE40_RS17705: ABIE40_RS17705 - GTP-binding protein, at 3,533,768 to 3,534,934
_RS17705
Position (kb)
3533
3534 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3532.539 kb on + strand, within ABIE40_RS17695 at 3532.540 kb on - strand, within ABIE40_RS17695 at 3532.540 kb on - strand, within ABIE40_RS17695 at 3532.614 kb on + strand, within ABIE40_RS17695 at 3532.614 kb on + strand, within ABIE40_RS17695 at 3532.614 kb on + strand, within ABIE40_RS17695 at 3532.615 kb on - strand, within ABIE40_RS17695 at 3532.615 kb on - strand, within ABIE40_RS17695 at 3532.615 kb on - strand, within ABIE40_RS17695 at 3532.653 kb on + strand, within ABIE40_RS17695 at 3532.654 kb on - strand, within ABIE40_RS17695 at 3532.654 kb on - strand, within ABIE40_RS17695 at 3532.656 kb on + strand, within ABIE40_RS17695 at 3532.656 kb on + strand, within ABIE40_RS17695 at 3532.657 kb on - strand, within ABIE40_RS17695 at 3532.671 kb on - strand, within ABIE40_RS17695 at 3532.689 kb on + strand, within ABIE40_RS17695 at 3532.689 kb on + strand, within ABIE40_RS17695 at 3532.689 kb on + strand, within ABIE40_RS17695 at 3532.690 kb on - strand, within ABIE40_RS17695 at 3532.690 kb on - strand, within ABIE40_RS17695 at 3532.690 kb on - strand, within ABIE40_RS17695 at 3532.690 kb on - strand, within ABIE40_RS17695 at 3532.690 kb on - strand, within ABIE40_RS17695 at 3532.759 kb on - strand, within ABIE40_RS17695 at 3532.791 kb on + strand, within ABIE40_RS17695 at 3532.791 kb on + strand, within ABIE40_RS17695 at 3532.791 kb on + strand, within ABIE40_RS17695 at 3532.791 kb on + strand, within ABIE40_RS17695 at 3532.792 kb on - strand, within ABIE40_RS17695 at 3532.792 kb on - strand, within ABIE40_RS17695 at 3532.792 kb on - strand, within ABIE40_RS17695 at 3532.792 kb on - strand, within ABIE40_RS17695 at 3532.878 kb on + strand, within ABIE40_RS17695 at 3532.878 kb on + strand, within ABIE40_RS17695 at 3532.878 kb on + strand, within ABIE40_RS17695 at 3532.878 kb on + strand, within ABIE40_RS17695 at 3532.878 kb on + strand, within ABIE40_RS17695 at 3532.878 kb on + strand, within ABIE40_RS17695 at 3532.879 kb on - strand, within ABIE40_RS17695 at 3532.879 kb on - strand, within ABIE40_RS17695 at 3532.879 kb on - strand, within ABIE40_RS17695 at 3532.879 kb on - strand, within ABIE40_RS17695 at 3532.879 kb on - strand, within ABIE40_RS17695 at 3532.879 kb on - strand, within ABIE40_RS17695 at 3532.902 kb on + strand, within ABIE40_RS17695 at 3532.902 kb on + strand, within ABIE40_RS17695 at 3532.989 kb on + strand, within ABIE40_RS17695 at 3532.989 kb on + strand, within ABIE40_RS17695 at 3532.989 kb on + strand, within ABIE40_RS17695 at 3532.989 kb on + strand, within ABIE40_RS17695 at 3532.989 kb on + strand, within ABIE40_RS17695 at 3532.990 kb on - strand, within ABIE40_RS17695 at 3532.990 kb on - strand, within ABIE40_RS17695 at 3533.091 kb on + strand, within ABIE40_RS17695 at 3533.092 kb on - strand, within ABIE40_RS17695 at 3533.104 kb on - strand, within ABIE40_RS17695 at 3533.115 kb on + strand, within ABIE40_RS17695 at 3533.115 kb on + strand, within ABIE40_RS17695 at 3533.115 kb on + strand, within ABIE40_RS17695 at 3533.116 kb on - strand, within ABIE40_RS17695 at 3533.154 kb on + strand, within ABIE40_RS17695 at 3533.154 kb on + strand, within ABIE40_RS17695 at 3533.220 kb on + strand, within ABIE40_RS17695 at 3533.220 kb on + strand, within ABIE40_RS17695 at 3533.555 kb on + strand, within ABIE40_RS17700 at 3533.555 kb on + strand, within ABIE40_RS17700 at 3533.555 kb on + strand, within ABIE40_RS17700 at 3533.555 kb on + strand, within ABIE40_RS17700 at 3533.555 kb on + strand, within ABIE40_RS17700 at 3533.556 kb on - strand, within ABIE40_RS17700 at 3533.556 kb on - strand, within ABIE40_RS17700 at 3533.556 kb on - strand, within ABIE40_RS17700 at 3533.556 kb on - strand, within ABIE40_RS17700 at 3533.556 kb on - strand, within ABIE40_RS17700 at 3533.556 kb on - strand, within ABIE40_RS17700 at 3533.762 kb on + strand at 3533.763 kb on - strand at 3533.763 kb on - strand at 3534.263 kb on + strand, within ABIE40_RS17705 at 3534.312 kb on + strand, within ABIE40_RS17705 at 3534.313 kb on - strand, within ABIE40_RS17705 at 3534.313 kb on - strand, within ABIE40_RS17705 at 3534.313 kb on - strand, within ABIE40_RS17705 at 3534.416 kb on + strand, within ABIE40_RS17705 at 3534.416 kb on + strand, within ABIE40_RS17705 at 3534.417 kb on - strand, within ABIE40_RS17705
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 15.9309 mM remove 3,532,539 + ABIE40_RS17695 0.21 -2.1 3,532,540 - ABIE40_RS17695 0.21 -3.8 3,532,540 - ABIE40_RS17695 0.21 -3.3 3,532,614 + ABIE40_RS17695 0.27 -0.7 3,532,614 + ABIE40_RS17695 0.27 -1.8 3,532,614 + ABIE40_RS17695 0.27 -2.3 3,532,615 - ABIE40_RS17695 0.27 -2.4 3,532,615 - ABIE40_RS17695 0.27 +1.5 3,532,615 - ABIE40_RS17695 0.27 -1.0 3,532,653 + ABIE40_RS17695 0.30 -2.9 3,532,654 - ABIE40_RS17695 0.30 -1.5 3,532,654 - ABIE40_RS17695 0.30 -2.1 3,532,656 + ABIE40_RS17695 0.30 -1.4 3,532,656 + ABIE40_RS17695 0.30 +0.2 3,532,657 - ABIE40_RS17695 0.30 -0.9 3,532,671 - ABIE40_RS17695 0.31 -1.5 3,532,689 + ABIE40_RS17695 0.33 -2.1 3,532,689 + ABIE40_RS17695 0.33 -2.7 3,532,689 + ABIE40_RS17695 0.33 -2.7 3,532,690 - ABIE40_RS17695 0.33 +1.8 3,532,690 - ABIE40_RS17695 0.33 +0.4 3,532,690 - ABIE40_RS17695 0.33 -3.8 3,532,690 - ABIE40_RS17695 0.33 -1.8 3,532,690 - ABIE40_RS17695 0.33 +1.8 3,532,759 - ABIE40_RS17695 0.38 -0.2 3,532,791 + ABIE40_RS17695 0.41 -1.2 3,532,791 + ABIE40_RS17695 0.41 -2.0 3,532,791 + ABIE40_RS17695 0.41 -0.5 3,532,791 + ABIE40_RS17695 0.41 -2.2 3,532,792 - ABIE40_RS17695 0.41 -3.2 3,532,792 - ABIE40_RS17695 0.41 +1.8 3,532,792 - ABIE40_RS17695 0.41 -2.1 3,532,792 - ABIE40_RS17695 0.41 +3.4 3,532,878 + ABIE40_RS17695 0.48 -1.7 3,532,878 + ABIE40_RS17695 0.48 -0.5 3,532,878 + ABIE40_RS17695 0.48 -0.2 3,532,878 + ABIE40_RS17695 0.48 -1.0 3,532,878 + ABIE40_RS17695 0.48 -2.7 3,532,878 + ABIE40_RS17695 0.48 -1.5 3,532,879 - ABIE40_RS17695 0.48 -0.5 3,532,879 - ABIE40_RS17695 0.48 +0.6 3,532,879 - ABIE40_RS17695 0.48 -2.2 3,532,879 - ABIE40_RS17695 0.48 -1.7 3,532,879 - ABIE40_RS17695 0.48 -2.5 3,532,879 - ABIE40_RS17695 0.48 -1.0 3,532,902 + ABIE40_RS17695 0.50 -1.7 3,532,902 + ABIE40_RS17695 0.50 -0.2 3,532,989 + ABIE40_RS17695 0.57 -1.0 3,532,989 + ABIE40_RS17695 0.57 +0.2 3,532,989 + ABIE40_RS17695 0.57 -1.3 3,532,989 + ABIE40_RS17695 0.57 -1.8 3,532,989 + ABIE40_RS17695 0.57 -1.8 3,532,990 - ABIE40_RS17695 0.57 -2.1 3,532,990 - ABIE40_RS17695 0.57 -1.2 3,533,091 + ABIE40_RS17695 0.65 -0.6 3,533,092 - ABIE40_RS17695 0.65 -3.6 3,533,104 - ABIE40_RS17695 0.66 +0.6 3,533,115 + ABIE40_RS17695 0.67 -3.5 3,533,115 + ABIE40_RS17695 0.67 +1.1 3,533,115 + ABIE40_RS17695 0.67 -2.3 3,533,116 - ABIE40_RS17695 0.67 +0.8 3,533,154 + ABIE40_RS17695 0.70 -1.9 3,533,154 + ABIE40_RS17695 0.70 +1.1 3,533,220 + ABIE40_RS17695 0.75 -0.2 3,533,220 + ABIE40_RS17695 0.75 -1.0 3,533,555 + ABIE40_RS17700 0.10 +0.3 3,533,555 + ABIE40_RS17700 0.10 +0.3 3,533,555 + ABIE40_RS17700 0.10 -0.7 3,533,555 + ABIE40_RS17700 0.10 -0.9 3,533,555 + ABIE40_RS17700 0.10 -1.4 3,533,556 - ABIE40_RS17700 0.11 -2.1 3,533,556 - ABIE40_RS17700 0.11 -2.1 3,533,556 - ABIE40_RS17700 0.11 -3.3 3,533,556 - ABIE40_RS17700 0.11 -3.8 3,533,556 - ABIE40_RS17700 0.11 -0.5 3,533,556 - ABIE40_RS17700 0.11 -1.4 3,533,762 + +2.6 3,533,763 - -0.1 3,533,763 - -0.5 3,534,263 + ABIE40_RS17705 0.42 -0.0 3,534,312 + ABIE40_RS17705 0.47 -3.0 3,534,313 - ABIE40_RS17705 0.47 +1.1 3,534,313 - ABIE40_RS17705 0.47 +0.6 3,534,313 - ABIE40_RS17705 0.47 +0.9 3,534,416 + ABIE40_RS17705 0.56 -2.2 3,534,416 + ABIE40_RS17705 0.56 +1.3 3,534,417 - ABIE40_RS17705 0.56 +0.0
Or see this region's nucleotide sequence