Experiment: R2A with 3-octanone 15.9309 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS17670 and ABIE40_RS17675 overlap by 4 nucleotides ABIE40_RS17675 and ABIE40_RS17680 are separated by 147 nucleotides ABIE40_RS17680 and ABIE40_RS17685 are separated by 4 nucleotides
ABIE40_RS17670: ABIE40_RS17670 - 3-dehydroquinate synthase, at 3,528,057 to 3,529,190
_RS17670
ABIE40_RS17675: ABIE40_RS17675 - shikimate kinase, at 3,529,187 to 3,529,777
_RS17675
ABIE40_RS17680: ABIE40_RS17680 - hypothetical protein, at 3,529,925 to 3,530,101
_RS17680
ABIE40_RS17685: ABIE40_RS17685 - site-specific tyrosine recombinase XerD, at 3,530,106 to 3,531,059
_RS17685
Position (kb)
3529
3530
3531 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3529.213 kb on - strand at 3529.213 kb on - strand at 3529.213 kb on - strand at 3529.784 kb on - strand at 3529.784 kb on - strand at 3529.784 kb on - strand at 3529.979 kb on + strand, within ABIE40_RS17680 at 3529.979 kb on + strand, within ABIE40_RS17680 at 3529.979 kb on + strand, within ABIE40_RS17680 at 3529.979 kb on + strand, within ABIE40_RS17680 at 3529.979 kb on + strand, within ABIE40_RS17680 at 3529.980 kb on - strand, within ABIE40_RS17680 at 3529.980 kb on - strand, within ABIE40_RS17680 at 3529.980 kb on - strand, within ABIE40_RS17680 at 3529.980 kb on - strand, within ABIE40_RS17680 at 3530.193 kb on + strand at 3530.193 kb on + strand at 3530.194 kb on - strand at 3530.194 kb on - strand at 3530.194 kb on - strand at 3530.281 kb on - strand, within ABIE40_RS17685 at 3530.281 kb on - strand, within ABIE40_RS17685 at 3530.355 kb on + strand, within ABIE40_RS17685 at 3530.355 kb on + strand, within ABIE40_RS17685 at 3530.368 kb on - strand, within ABIE40_RS17685 at 3530.531 kb on + strand, within ABIE40_RS17685 at 3530.531 kb on + strand, within ABIE40_RS17685 at 3530.531 kb on + strand, within ABIE40_RS17685 at 3530.532 kb on - strand, within ABIE40_RS17685 at 3530.532 kb on - strand, within ABIE40_RS17685 at 3530.532 kb on - strand, within ABIE40_RS17685 at 3530.532 kb on - strand, within ABIE40_RS17685 at 3530.532 kb on - strand, within ABIE40_RS17685 at 3530.557 kb on - strand, within ABIE40_RS17685 at 3530.703 kb on + strand, within ABIE40_RS17685 at 3530.704 kb on - strand, within ABIE40_RS17685 at 3530.704 kb on - strand, within ABIE40_RS17685 at 3530.704 kb on - strand, within ABIE40_RS17685 at 3530.736 kb on - strand, within ABIE40_RS17685 at 3530.736 kb on - strand, within ABIE40_RS17685 at 3530.790 kb on + strand, within ABIE40_RS17685 at 3530.790 kb on + strand, within ABIE40_RS17685 at 3530.790 kb on + strand, within ABIE40_RS17685 at 3530.790 kb on + strand, within ABIE40_RS17685 at 3530.790 kb on + strand, within ABIE40_RS17685 at 3530.791 kb on - strand, within ABIE40_RS17685 at 3530.791 kb on - strand, within ABIE40_RS17685 at 3530.791 kb on - strand, within ABIE40_RS17685 at 3530.791 kb on - strand, within ABIE40_RS17685 at 3530.791 kb on - strand, within ABIE40_RS17685 at 3530.791 kb on - strand, within ABIE40_RS17685 at 3530.976 kb on + strand at 3530.976 kb on + strand at 3530.976 kb on + strand at 3530.976 kb on + strand at 3530.977 kb on - strand at 3530.977 kb on - strand at 3530.979 kb on - strand at 3531.057 kb on + strand at 3531.057 kb on + strand at 3531.057 kb on + strand at 3531.057 kb on + strand at 3531.082 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 15.9309 mM remove 3,529,213 - -0.7 3,529,213 - +0.3 3,529,213 - +0.8 3,529,784 - +0.7 3,529,784 - +0.1 3,529,784 - +0.0 3,529,979 + ABIE40_RS17680 0.31 +0.1 3,529,979 + ABIE40_RS17680 0.31 -1.0 3,529,979 + ABIE40_RS17680 0.31 -0.1 3,529,979 + ABIE40_RS17680 0.31 -2.4 3,529,979 + ABIE40_RS17680 0.31 -0.9 3,529,980 - ABIE40_RS17680 0.31 -3.5 3,529,980 - ABIE40_RS17680 0.31 -3.3 3,529,980 - ABIE40_RS17680 0.31 +0.3 3,529,980 - ABIE40_RS17680 0.31 -2.2 3,530,193 + -2.3 3,530,193 + -1.0 3,530,194 - -0.7 3,530,194 - +0.8 3,530,194 - +0.2 3,530,281 - ABIE40_RS17685 0.18 -3.0 3,530,281 - ABIE40_RS17685 0.18 +1.2 3,530,355 + ABIE40_RS17685 0.26 -2.2 3,530,355 + ABIE40_RS17685 0.26 -1.0 3,530,368 - ABIE40_RS17685 0.27 -0.1 3,530,531 + ABIE40_RS17685 0.45 -0.7 3,530,531 + ABIE40_RS17685 0.45 -0.2 3,530,531 + ABIE40_RS17685 0.45 -1.1 3,530,532 - ABIE40_RS17685 0.45 -1.7 3,530,532 - ABIE40_RS17685 0.45 +0.0 3,530,532 - ABIE40_RS17685 0.45 -2.8 3,530,532 - ABIE40_RS17685 0.45 -0.1 3,530,532 - ABIE40_RS17685 0.45 -0.7 3,530,557 - ABIE40_RS17685 0.47 -1.0 3,530,703 + ABIE40_RS17685 0.63 +0.7 3,530,704 - ABIE40_RS17685 0.63 -0.2 3,530,704 - ABIE40_RS17685 0.63 -0.4 3,530,704 - ABIE40_RS17685 0.63 -0.3 3,530,736 - ABIE40_RS17685 0.66 -2.2 3,530,736 - ABIE40_RS17685 0.66 -2.0 3,530,790 + ABIE40_RS17685 0.72 +0.2 3,530,790 + ABIE40_RS17685 0.72 +0.7 3,530,790 + ABIE40_RS17685 0.72 -1.8 3,530,790 + ABIE40_RS17685 0.72 -2.3 3,530,790 + ABIE40_RS17685 0.72 -1.9 3,530,791 - ABIE40_RS17685 0.72 +0.2 3,530,791 - ABIE40_RS17685 0.72 +0.3 3,530,791 - ABIE40_RS17685 0.72 +0.8 3,530,791 - ABIE40_RS17685 0.72 -0.5 3,530,791 - ABIE40_RS17685 0.72 +2.7 3,530,791 - ABIE40_RS17685 0.72 -3.2 3,530,976 + -1.0 3,530,976 + -3.0 3,530,976 + +0.2 3,530,976 + -2.1 3,530,977 - +3.0 3,530,977 - -2.4 3,530,979 - -0.6 3,531,057 + -1.2 3,531,057 + -1.5 3,531,057 + -1.0 3,531,057 + -0.5 3,531,082 + -0.6
Or see this region's nucleotide sequence