Experiment: R2A with 3-octanone 15.9309 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS14240 and ABIE40_RS14245 overlap by 11 nucleotides ABIE40_RS14245 and ABIE40_RS14250 are separated by 188 nucleotides ABIE40_RS14250 and ABIE40_RS14255 are separated by 30 nucleotides ABIE40_RS14255 and ABIE40_RS14260 are separated by 52 nucleotides
ABIE40_RS14240: ABIE40_RS14240 - sugar transferase, at 2,783,110 to 2,783,802
_RS14240
ABIE40_RS14245: ABIE40_RS14245 - hypothetical protein, at 2,783,792 to 2,783,992
_RS14245
ABIE40_RS14250: ABIE40_RS14250 - oligosaccharide flippase family protein, at 2,784,181 to 2,785,698
_RS14250
ABIE40_RS14255: ABIE40_RS14255 - methyltransferase, TIGR04325 family, at 2,785,729 to 2,786,547
_RS14255
ABIE40_RS14260: ABIE40_RS14260 - GMC family oxidoreductase, at 2,786,600 to 2,788,297
_RS14260
Position (kb)
2784
2785
2786 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2783.323 kb on + strand, within ABIE40_RS14240 at 2783.324 kb on - strand, within ABIE40_RS14240 at 2783.588 kb on - strand, within ABIE40_RS14240 at 2783.608 kb on + strand, within ABIE40_RS14240 at 2783.608 kb on + strand, within ABIE40_RS14240 at 2783.608 kb on + strand, within ABIE40_RS14240 at 2783.608 kb on + strand, within ABIE40_RS14240 at 2783.608 kb on + strand, within ABIE40_RS14240 at 2783.609 kb on - strand, within ABIE40_RS14240 at 2783.609 kb on - strand, within ABIE40_RS14240 at 2783.609 kb on - strand, within ABIE40_RS14240 at 2783.609 kb on - strand, within ABIE40_RS14240 at 2783.671 kb on + strand, within ABIE40_RS14240 at 2783.672 kb on - strand, within ABIE40_RS14240 at 2783.672 kb on - strand, within ABIE40_RS14240 at 2783.672 kb on - strand, within ABIE40_RS14240 at 2783.672 kb on - strand, within ABIE40_RS14240 at 2783.672 kb on - strand, within ABIE40_RS14240 at 2783.690 kb on - strand, within ABIE40_RS14240 at 2783.702 kb on - strand, within ABIE40_RS14240 at 2783.704 kb on + strand, within ABIE40_RS14240 at 2783.705 kb on - strand, within ABIE40_RS14240 at 2783.705 kb on - strand, within ABIE40_RS14240 at 2783.858 kb on + strand, within ABIE40_RS14245 at 2783.865 kb on + strand, within ABIE40_RS14245 at 2783.866 kb on - strand, within ABIE40_RS14245 at 2783.955 kb on + strand, within ABIE40_RS14245 at 2783.956 kb on - strand, within ABIE40_RS14245 at 2784.029 kb on + strand at 2784.029 kb on + strand at 2784.030 kb on - strand at 2784.030 kb on - strand at 2784.030 kb on - strand at 2784.030 kb on - strand at 2784.133 kb on + strand at 2784.364 kb on + strand, within ABIE40_RS14250 at 2784.364 kb on + strand, within ABIE40_RS14250 at 2784.364 kb on + strand, within ABIE40_RS14250 at 2784.364 kb on + strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.365 kb on - strand, within ABIE40_RS14250 at 2784.370 kb on + strand, within ABIE40_RS14250 at 2784.371 kb on - strand, within ABIE40_RS14250 at 2784.372 kb on + strand, within ABIE40_RS14250 at 2784.372 kb on + strand, within ABIE40_RS14250 at 2784.372 kb on + strand, within ABIE40_RS14250 at 2784.372 kb on + strand, within ABIE40_RS14250 at 2784.373 kb on - strand, within ABIE40_RS14250 at 2784.373 kb on - strand, within ABIE40_RS14250 at 2784.373 kb on - strand, within ABIE40_RS14250 at 2784.373 kb on - strand, within ABIE40_RS14250 at 2784.373 kb on - strand, within ABIE40_RS14250 at 2784.373 kb on - strand, within ABIE40_RS14250 at 2784.373 kb on - strand, within ABIE40_RS14250 at 2784.373 kb on - strand, within ABIE40_RS14250 at 2784.373 kb on - strand, within ABIE40_RS14250 at 2784.427 kb on + strand, within ABIE40_RS14250 at 2784.427 kb on + strand, within ABIE40_RS14250 at 2784.428 kb on - strand, within ABIE40_RS14250 at 2784.428 kb on - strand, within ABIE40_RS14250 at 2784.428 kb on - strand, within ABIE40_RS14250 at 2784.428 kb on - strand, within ABIE40_RS14250 at 2784.542 kb on - strand, within ABIE40_RS14250 at 2784.580 kb on + strand, within ABIE40_RS14250 at 2784.580 kb on + strand, within ABIE40_RS14250 at 2784.581 kb on - strand, within ABIE40_RS14250 at 2784.581 kb on - strand, within ABIE40_RS14250 at 2784.581 kb on - strand, within ABIE40_RS14250 at 2784.581 kb on - strand, within ABIE40_RS14250 at 2784.582 kb on + strand, within ABIE40_RS14250 at 2784.582 kb on + strand, within ABIE40_RS14250 at 2784.582 kb on + strand, within ABIE40_RS14250 at 2784.582 kb on + strand, within ABIE40_RS14250 at 2784.582 kb on + strand, within ABIE40_RS14250 at 2784.583 kb on - strand, within ABIE40_RS14250 at 2784.583 kb on - strand, within ABIE40_RS14250 at 2784.583 kb on - strand, within ABIE40_RS14250 at 2784.583 kb on - strand, within ABIE40_RS14250 at 2784.583 kb on - strand, within ABIE40_RS14250 at 2784.583 kb on - strand, within ABIE40_RS14250 at 2784.892 kb on + strand, within ABIE40_RS14250 at 2784.892 kb on + strand, within ABIE40_RS14250 at 2784.893 kb on - strand, within ABIE40_RS14250 at 2784.893 kb on - strand, within ABIE40_RS14250 at 2784.905 kb on - strand, within ABIE40_RS14250 at 2784.905 kb on - strand, within ABIE40_RS14250 at 2784.959 kb on - strand, within ABIE40_RS14250 at 2784.959 kb on - strand, within ABIE40_RS14250 at 2784.959 kb on - strand, within ABIE40_RS14250 at 2784.959 kb on - strand, within ABIE40_RS14250 at 2784.990 kb on - strand, within ABIE40_RS14250 at 2785.141 kb on + strand, within ABIE40_RS14250 at 2785.141 kb on + strand, within ABIE40_RS14250 at 2785.142 kb on - strand, within ABIE40_RS14250 at 2785.142 kb on - strand, within ABIE40_RS14250 at 2785.142 kb on - strand, within ABIE40_RS14250 at 2785.142 kb on - strand, within ABIE40_RS14250 at 2785.142 kb on - strand, within ABIE40_RS14250 at 2785.142 kb on - strand, within ABIE40_RS14250 at 2785.261 kb on + strand, within ABIE40_RS14250 at 2785.262 kb on - strand, within ABIE40_RS14250 at 2785.262 kb on - strand, within ABIE40_RS14250 at 2785.492 kb on + strand, within ABIE40_RS14250 at 2785.493 kb on - strand, within ABIE40_RS14250 at 2785.496 kb on + strand, within ABIE40_RS14250 at 2785.496 kb on + strand, within ABIE40_RS14250 at 2785.496 kb on + strand, within ABIE40_RS14250 at 2785.496 kb on + strand, within ABIE40_RS14250 at 2785.496 kb on + strand, within ABIE40_RS14250 at 2785.496 kb on + strand, within ABIE40_RS14250 at 2785.497 kb on - strand, within ABIE40_RS14250 at 2785.497 kb on - strand, within ABIE40_RS14250 at 2785.497 kb on - strand, within ABIE40_RS14250 at 2785.497 kb on - strand, within ABIE40_RS14250 at 2785.497 kb on - strand, within ABIE40_RS14250 at 2785.497 kb on - strand, within ABIE40_RS14250 at 2785.497 kb on - strand, within ABIE40_RS14250 at 2785.531 kb on + strand, within ABIE40_RS14250 at 2785.531 kb on + strand, within ABIE40_RS14250 at 2785.531 kb on + strand, within ABIE40_RS14250 at 2785.543 kb on + strand, within ABIE40_RS14250 at 2785.543 kb on + strand, within ABIE40_RS14250 at 2785.544 kb on - strand, within ABIE40_RS14250 at 2785.545 kb on + strand, within ABIE40_RS14250 at 2785.615 kb on + strand at 2785.615 kb on + strand at 2785.616 kb on - strand at 2785.644 kb on + strand at 2785.644 kb on + strand at 2785.644 kb on + strand at 2785.644 kb on + strand at 2785.644 kb on + strand at 2785.645 kb on - strand at 2785.645 kb on - strand at 2785.645 kb on - strand at 2785.645 kb on - strand at 2785.645 kb on - strand at 2785.696 kb on + strand at 2785.697 kb on - strand at 2785.697 kb on - strand at 2785.697 kb on - strand at 2785.697 kb on - strand at 2785.697 kb on - strand at 2785.697 kb on - strand at 2785.703 kb on + strand at 2785.703 kb on + strand at 2785.703 kb on + strand at 2785.703 kb on + strand at 2785.704 kb on - strand at 2785.802 kb on + strand at 2785.802 kb on + strand at 2785.803 kb on - strand at 2785.835 kb on - strand, within ABIE40_RS14255 at 2785.870 kb on + strand, within ABIE40_RS14255 at 2785.870 kb on + strand, within ABIE40_RS14255 at 2785.870 kb on + strand, within ABIE40_RS14255 at 2785.879 kb on + strand, within ABIE40_RS14255 at 2785.879 kb on + strand, within ABIE40_RS14255 at 2786.123 kb on - strand, within ABIE40_RS14255 at 2786.123 kb on - strand, within ABIE40_RS14255 at 2786.254 kb on + strand, within ABIE40_RS14255 at 2786.254 kb on + strand, within ABIE40_RS14255 at 2786.254 kb on + strand, within ABIE40_RS14255 at 2786.310 kb on + strand, within ABIE40_RS14255 at 2786.310 kb on + strand, within ABIE40_RS14255 at 2786.311 kb on - strand, within ABIE40_RS14255 at 2786.311 kb on - strand, within ABIE40_RS14255 at 2786.311 kb on - strand, within ABIE40_RS14255 at 2786.311 kb on - strand, within ABIE40_RS14255 at 2786.384 kb on + strand, within ABIE40_RS14255 at 2786.384 kb on + strand, within ABIE40_RS14255 at 2786.390 kb on - strand, within ABIE40_RS14255 at 2786.440 kb on + strand, within ABIE40_RS14255 at 2786.531 kb on - strand at 2786.553 kb on + strand at 2786.553 kb on + strand at 2786.553 kb on + strand at 2786.553 kb on + strand at 2786.553 kb on + strand at 2786.553 kb on + strand at 2786.553 kb on + strand at 2786.553 kb on + strand at 2786.554 kb on - strand at 2786.554 kb on - strand at 2786.554 kb on - strand at 2786.554 kb on - strand at 2786.554 kb on - strand at 2786.554 kb on - strand at 2786.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 15.9309 mM remove 2,783,323 + ABIE40_RS14240 0.31 -1.1 2,783,324 - ABIE40_RS14240 0.31 -2.4 2,783,588 - ABIE40_RS14240 0.69 +0.7 2,783,608 + ABIE40_RS14240 0.72 -1.6 2,783,608 + ABIE40_RS14240 0.72 -1.1 2,783,608 + ABIE40_RS14240 0.72 -1.1 2,783,608 + ABIE40_RS14240 0.72 -1.3 2,783,608 + ABIE40_RS14240 0.72 -0.7 2,783,609 - ABIE40_RS14240 0.72 -1.6 2,783,609 - ABIE40_RS14240 0.72 -0.5 2,783,609 - ABIE40_RS14240 0.72 -1.8 2,783,609 - ABIE40_RS14240 0.72 +1.0 2,783,671 + ABIE40_RS14240 0.81 -1.3 2,783,672 - ABIE40_RS14240 0.81 -1.3 2,783,672 - ABIE40_RS14240 0.81 -0.2 2,783,672 - ABIE40_RS14240 0.81 -1.8 2,783,672 - ABIE40_RS14240 0.81 +1.4 2,783,672 - ABIE40_RS14240 0.81 -1.6 2,783,690 - ABIE40_RS14240 0.84 -0.1 2,783,702 - ABIE40_RS14240 0.85 -0.6 2,783,704 + ABIE40_RS14240 0.86 -1.4 2,783,705 - ABIE40_RS14240 0.86 +0.1 2,783,705 - ABIE40_RS14240 0.86 -1.2 2,783,858 + ABIE40_RS14245 0.33 +0.9 2,783,865 + ABIE40_RS14245 0.36 -0.8 2,783,866 - ABIE40_RS14245 0.37 -0.7 2,783,955 + ABIE40_RS14245 0.81 -0.1 2,783,956 - ABIE40_RS14245 0.82 -0.7 2,784,029 + -0.2 2,784,029 + -2.0 2,784,030 - +1.0 2,784,030 - -1.1 2,784,030 - +0.3 2,784,030 - +1.3 2,784,133 + -1.5 2,784,364 + ABIE40_RS14250 0.12 -0.9 2,784,364 + ABIE40_RS14250 0.12 -1.7 2,784,364 + ABIE40_RS14250 0.12 -0.4 2,784,364 + ABIE40_RS14250 0.12 +0.9 2,784,365 - ABIE40_RS14250 0.12 +0.1 2,784,365 - ABIE40_RS14250 0.12 +2.4 2,784,365 - ABIE40_RS14250 0.12 +0.9 2,784,365 - ABIE40_RS14250 0.12 -1.5 2,784,365 - ABIE40_RS14250 0.12 -0.1 2,784,365 - ABIE40_RS14250 0.12 -3.2 2,784,365 - ABIE40_RS14250 0.12 -1.0 2,784,365 - ABIE40_RS14250 0.12 +2.4 2,784,365 - ABIE40_RS14250 0.12 +0.9 2,784,365 - ABIE40_RS14250 0.12 -3.1 2,784,365 - ABIE40_RS14250 0.12 +0.3 2,784,365 - ABIE40_RS14250 0.12 -0.6 2,784,365 - ABIE40_RS14250 0.12 -0.5 2,784,365 - ABIE40_RS14250 0.12 -0.6 2,784,370 + ABIE40_RS14250 0.12 -0.1 2,784,371 - ABIE40_RS14250 0.13 -0.2 2,784,372 + ABIE40_RS14250 0.13 -2.6 2,784,372 + ABIE40_RS14250 0.13 +0.2 2,784,372 + ABIE40_RS14250 0.13 -0.9 2,784,372 + ABIE40_RS14250 0.13 -0.7 2,784,373 - ABIE40_RS14250 0.13 -1.1 2,784,373 - ABIE40_RS14250 0.13 -0.6 2,784,373 - ABIE40_RS14250 0.13 -1.4 2,784,373 - ABIE40_RS14250 0.13 -1.1 2,784,373 - ABIE40_RS14250 0.13 -0.8 2,784,373 - ABIE40_RS14250 0.13 +0.5 2,784,373 - ABIE40_RS14250 0.13 -0.6 2,784,373 - ABIE40_RS14250 0.13 +0.7 2,784,373 - ABIE40_RS14250 0.13 -0.3 2,784,427 + ABIE40_RS14250 0.16 -0.7 2,784,427 + ABIE40_RS14250 0.16 -0.9 2,784,428 - ABIE40_RS14250 0.16 -0.9 2,784,428 - ABIE40_RS14250 0.16 +0.1 2,784,428 - ABIE40_RS14250 0.16 +0.1 2,784,428 - ABIE40_RS14250 0.16 +0.8 2,784,542 - ABIE40_RS14250 0.24 -0.5 2,784,580 + ABIE40_RS14250 0.26 +1.9 2,784,580 + ABIE40_RS14250 0.26 -0.2 2,784,581 - ABIE40_RS14250 0.26 +0.0 2,784,581 - ABIE40_RS14250 0.26 -2.4 2,784,581 - ABIE40_RS14250 0.26 -1.4 2,784,581 - ABIE40_RS14250 0.26 -0.6 2,784,582 + ABIE40_RS14250 0.26 +0.0 2,784,582 + ABIE40_RS14250 0.26 -0.7 2,784,582 + ABIE40_RS14250 0.26 -0.9 2,784,582 + ABIE40_RS14250 0.26 +0.1 2,784,582 + ABIE40_RS14250 0.26 -2.1 2,784,583 - ABIE40_RS14250 0.26 -1.1 2,784,583 - ABIE40_RS14250 0.26 +2.1 2,784,583 - ABIE40_RS14250 0.26 -0.4 2,784,583 - ABIE40_RS14250 0.26 -0.9 2,784,583 - ABIE40_RS14250 0.26 -0.2 2,784,583 - ABIE40_RS14250 0.26 +0.9 2,784,892 + ABIE40_RS14250 0.47 -0.4 2,784,892 + ABIE40_RS14250 0.47 -2.5 2,784,893 - ABIE40_RS14250 0.47 -0.9 2,784,893 - ABIE40_RS14250 0.47 +0.0 2,784,905 - ABIE40_RS14250 0.48 +2.2 2,784,905 - ABIE40_RS14250 0.48 -1.9 2,784,959 - ABIE40_RS14250 0.51 -1.5 2,784,959 - ABIE40_RS14250 0.51 +0.7 2,784,959 - ABIE40_RS14250 0.51 +0.6 2,784,959 - ABIE40_RS14250 0.51 -0.8 2,784,990 - ABIE40_RS14250 0.53 +0.3 2,785,141 + ABIE40_RS14250 0.63 +0.3 2,785,141 + ABIE40_RS14250 0.63 -0.5 2,785,142 - ABIE40_RS14250 0.63 -0.1 2,785,142 - ABIE40_RS14250 0.63 -1.4 2,785,142 - ABIE40_RS14250 0.63 -0.3 2,785,142 - ABIE40_RS14250 0.63 +0.5 2,785,142 - ABIE40_RS14250 0.63 -1.3 2,785,142 - ABIE40_RS14250 0.63 +0.9 2,785,261 + ABIE40_RS14250 0.71 -1.6 2,785,262 - ABIE40_RS14250 0.71 -1.1 2,785,262 - ABIE40_RS14250 0.71 -0.3 2,785,492 + ABIE40_RS14250 0.86 -0.6 2,785,493 - ABIE40_RS14250 0.86 +0.3 2,785,496 + ABIE40_RS14250 0.87 +0.0 2,785,496 + ABIE40_RS14250 0.87 +2.9 2,785,496 + ABIE40_RS14250 0.87 -0.8 2,785,496 + ABIE40_RS14250 0.87 -0.9 2,785,496 + ABIE40_RS14250 0.87 +0.6 2,785,496 + ABIE40_RS14250 0.87 -0.4 2,785,497 - ABIE40_RS14250 0.87 -1.2 2,785,497 - ABIE40_RS14250 0.87 -2.3 2,785,497 - ABIE40_RS14250 0.87 +1.9 2,785,497 - ABIE40_RS14250 0.87 +0.5 2,785,497 - ABIE40_RS14250 0.87 -0.3 2,785,497 - ABIE40_RS14250 0.87 -0.4 2,785,497 - ABIE40_RS14250 0.87 -0.8 2,785,531 + ABIE40_RS14250 0.89 -0.5 2,785,531 + ABIE40_RS14250 0.89 +0.2 2,785,531 + ABIE40_RS14250 0.89 -1.3 2,785,543 + ABIE40_RS14250 0.90 +0.6 2,785,543 + ABIE40_RS14250 0.90 -2.1 2,785,544 - ABIE40_RS14250 0.90 -2.5 2,785,545 + ABIE40_RS14250 0.90 +1.9 2,785,615 + -0.5 2,785,615 + +0.9 2,785,616 - -0.8 2,785,644 + -0.2 2,785,644 + -0.4 2,785,644 + +1.2 2,785,644 + +0.2 2,785,644 + +0.1 2,785,645 - -0.0 2,785,645 - -0.2 2,785,645 - +1.7 2,785,645 - +0.6 2,785,645 - +0.6 2,785,696 + -0.8 2,785,697 - -0.2 2,785,697 - -0.1 2,785,697 - +1.7 2,785,697 - -1.1 2,785,697 - -0.4 2,785,697 - -0.0 2,785,703 + +0.3 2,785,703 + -0.1 2,785,703 + -2.6 2,785,703 + -2.3 2,785,704 - +0.4 2,785,802 + -0.4 2,785,802 + -0.3 2,785,803 - -1.0 2,785,835 - ABIE40_RS14255 0.13 -1.1 2,785,870 + ABIE40_RS14255 0.17 -0.1 2,785,870 + ABIE40_RS14255 0.17 -2.0 2,785,870 + ABIE40_RS14255 0.17 +2.6 2,785,879 + ABIE40_RS14255 0.18 -3.2 2,785,879 + ABIE40_RS14255 0.18 +0.7 2,786,123 - ABIE40_RS14255 0.48 -0.0 2,786,123 - ABIE40_RS14255 0.48 +0.9 2,786,254 + ABIE40_RS14255 0.64 +1.2 2,786,254 + ABIE40_RS14255 0.64 +1.5 2,786,254 + ABIE40_RS14255 0.64 +0.4 2,786,310 + ABIE40_RS14255 0.71 +0.1 2,786,310 + ABIE40_RS14255 0.71 -1.6 2,786,311 - ABIE40_RS14255 0.71 -0.4 2,786,311 - ABIE40_RS14255 0.71 -2.2 2,786,311 - ABIE40_RS14255 0.71 +0.8 2,786,311 - ABIE40_RS14255 0.71 -1.8 2,786,384 + ABIE40_RS14255 0.80 +1.1 2,786,384 + ABIE40_RS14255 0.80 -0.3 2,786,390 - ABIE40_RS14255 0.81 -0.6 2,786,440 + ABIE40_RS14255 0.87 +0.6 2,786,531 - +0.3 2,786,553 + +0.2 2,786,553 + +0.7 2,786,553 + +0.3 2,786,553 + +1.4 2,786,553 + +0.5 2,786,553 + +0.5 2,786,553 + -0.3 2,786,553 + +0.0 2,786,554 - +3.3 2,786,554 - -2.0 2,786,554 - +0.1 2,786,554 - -1.2 2,786,554 - -2.4 2,786,554 - +0.9 2,786,554 - -2.3
Or see this region's nucleotide sequence