Experiment: R2A with 3-octanone 15.9309 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS03625 and ABIE40_RS03630 are separated by 71 nucleotides ABIE40_RS03630 and ABIE40_RS03635 are separated by 43 nucleotides ABIE40_RS03635 and ABIE40_RS03640 overlap by 38 nucleotides ABIE40_RS03640 and ABIE40_RS03645 are separated by 66 nucleotides
ABIE40_RS03625: ABIE40_RS03625 - DUF305 domain-containing protein, at 718,865 to 719,221
_RS03625
ABIE40_RS03630: ABIE40_RS03630 - DUF2946 family protein, at 719,293 to 719,658
_RS03630
ABIE40_RS03635: ABIE40_RS03635 - BrnA antitoxin family protein, at 719,702 to 720,013
_RS03635
ABIE40_RS03640: ABIE40_RS03640 - BrnT family toxin, at 719,976 to 720,254
_RS03640
ABIE40_RS03645: ABIE40_RS03645 - hypothetical protein, at 720,321 to 721,082
_RS03645
Position (kb) 719
720
721 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 718.747 kb on + strand at 718.748 kb on - strand at 718.750 kb on + strand at 718.751 kb on - strand at 718.870 kb on + strand at 718.968 kb on + strand, within ABIE40_RS03625 at 718.968 kb on + strand, within ABIE40_RS03625 at 719.143 kb on + strand, within ABIE40_RS03625 at 719.143 kb on + strand, within ABIE40_RS03625 at 719.143 kb on + strand, within ABIE40_RS03625 at 719.143 kb on + strand, within ABIE40_RS03625 at 719.143 kb on + strand, within ABIE40_RS03625 at 719.143 kb on + strand, within ABIE40_RS03625 at 719.143 kb on + strand, within ABIE40_RS03625 at 719.144 kb on - strand, within ABIE40_RS03625 at 719.144 kb on - strand, within ABIE40_RS03625 at 719.144 kb on - strand, within ABIE40_RS03625 at 719.144 kb on - strand, within ABIE40_RS03625 at 719.144 kb on - strand, within ABIE40_RS03625 at 719.144 kb on - strand, within ABIE40_RS03625 at 719.200 kb on + strand at 719.200 kb on + strand at 719.200 kb on + strand at 719.201 kb on - strand at 719.216 kb on - strand at 719.216 kb on - strand at 719.216 kb on - strand at 719.278 kb on + strand at 719.279 kb on - strand at 719.429 kb on + strand, within ABIE40_RS03630 at 719.429 kb on + strand, within ABIE40_RS03630 at 719.430 kb on - strand, within ABIE40_RS03630 at 719.508 kb on + strand, within ABIE40_RS03630 at 719.508 kb on + strand, within ABIE40_RS03630 at 719.508 kb on + strand, within ABIE40_RS03630 at 719.508 kb on + strand, within ABIE40_RS03630 at 719.508 kb on + strand, within ABIE40_RS03630 at 719.509 kb on - strand, within ABIE40_RS03630 at 719.509 kb on - strand, within ABIE40_RS03630 at 719.509 kb on - strand, within ABIE40_RS03630 at 719.509 kb on - strand, within ABIE40_RS03630 at 719.521 kb on - strand, within ABIE40_RS03630 at 719.629 kb on + strand at 719.629 kb on + strand at 719.629 kb on + strand at 719.629 kb on + strand at 719.630 kb on - strand at 719.669 kb on + strand at 719.669 kb on + strand at 719.669 kb on + strand at 719.669 kb on + strand at 719.669 kb on + strand at 719.670 kb on - strand at 719.670 kb on - strand at 720.014 kb on - strand, within ABIE40_RS03640 at 720.183 kb on + strand, within ABIE40_RS03640 at 720.184 kb on - strand, within ABIE40_RS03640 at 720.311 kb on - strand at 720.355 kb on + strand at 720.356 kb on - strand at 720.408 kb on + strand, within ABIE40_RS03645 at 720.408 kb on + strand, within ABIE40_RS03645 at 720.408 kb on + strand, within ABIE40_RS03645 at 720.409 kb on - strand, within ABIE40_RS03645 at 720.409 kb on - strand, within ABIE40_RS03645 at 720.409 kb on - strand, within ABIE40_RS03645 at 720.409 kb on - strand, within ABIE40_RS03645 at 720.409 kb on - strand, within ABIE40_RS03645 at 720.409 kb on - strand, within ABIE40_RS03645 at 720.409 kb on - strand, within ABIE40_RS03645 at 720.409 kb on - strand, within ABIE40_RS03645 at 720.409 kb on - strand, within ABIE40_RS03645 at 720.484 kb on + strand, within ABIE40_RS03645 at 720.484 kb on + strand, within ABIE40_RS03645 at 720.484 kb on + strand, within ABIE40_RS03645 at 720.484 kb on + strand, within ABIE40_RS03645 at 720.484 kb on + strand, within ABIE40_RS03645 at 720.484 kb on + strand, within ABIE40_RS03645 at 720.485 kb on - strand, within ABIE40_RS03645 at 720.485 kb on - strand, within ABIE40_RS03645 at 720.486 kb on - strand, within ABIE40_RS03645 at 720.492 kb on - strand, within ABIE40_RS03645 at 720.492 kb on - strand, within ABIE40_RS03645 at 720.492 kb on - strand, within ABIE40_RS03645 at 720.492 kb on - strand, within ABIE40_RS03645 at 720.492 kb on - strand, within ABIE40_RS03645 at 720.539 kb on + strand, within ABIE40_RS03645 at 720.540 kb on - strand, within ABIE40_RS03645 at 720.540 kb on - strand, within ABIE40_RS03645 at 720.540 kb on - strand, within ABIE40_RS03645 at 720.540 kb on - strand, within ABIE40_RS03645 at 720.566 kb on + strand, within ABIE40_RS03645 at 720.567 kb on - strand, within ABIE40_RS03645 at 720.665 kb on - strand, within ABIE40_RS03645 at 720.724 kb on + strand, within ABIE40_RS03645 at 720.828 kb on - strand, within ABIE40_RS03645
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 15.9309 mM remove 718,747 + -2.1 718,748 - -1.8 718,750 + +0.4 718,751 - +0.5 718,870 + -0.9 718,968 + ABIE40_RS03625 0.29 -0.6 718,968 + ABIE40_RS03625 0.29 +2.5 719,143 + ABIE40_RS03625 0.78 -0.1 719,143 + ABIE40_RS03625 0.78 -0.3 719,143 + ABIE40_RS03625 0.78 +0.5 719,143 + ABIE40_RS03625 0.78 -0.8 719,143 + ABIE40_RS03625 0.78 +0.5 719,143 + ABIE40_RS03625 0.78 -0.9 719,143 + ABIE40_RS03625 0.78 +0.4 719,144 - ABIE40_RS03625 0.78 +1.0 719,144 - ABIE40_RS03625 0.78 -0.1 719,144 - ABIE40_RS03625 0.78 +0.9 719,144 - ABIE40_RS03625 0.78 -0.8 719,144 - ABIE40_RS03625 0.78 +1.2 719,144 - ABIE40_RS03625 0.78 -1.8 719,200 + +0.4 719,200 + +1.0 719,200 + +0.6 719,201 - -0.1 719,216 - -0.0 719,216 - -0.1 719,216 - -0.4 719,278 + -1.3 719,279 - +0.2 719,429 + ABIE40_RS03630 0.37 +2.3 719,429 + ABIE40_RS03630 0.37 -0.7 719,430 - ABIE40_RS03630 0.37 -2.3 719,508 + ABIE40_RS03630 0.59 -2.9 719,508 + ABIE40_RS03630 0.59 +0.3 719,508 + ABIE40_RS03630 0.59 -0.1 719,508 + ABIE40_RS03630 0.59 +0.2 719,508 + ABIE40_RS03630 0.59 -0.4 719,509 - ABIE40_RS03630 0.59 -0.5 719,509 - ABIE40_RS03630 0.59 +1.0 719,509 - ABIE40_RS03630 0.59 +2.9 719,509 - ABIE40_RS03630 0.59 -2.8 719,521 - ABIE40_RS03630 0.62 +0.2 719,629 + -2.2 719,629 + -2.1 719,629 + -1.0 719,629 + +0.2 719,630 - +1.1 719,669 + +0.3 719,669 + -2.1 719,669 + -0.4 719,669 + -1.7 719,669 + -0.7 719,670 - +1.9 719,670 - +0.2 720,014 - ABIE40_RS03640 0.14 -0.0 720,183 + ABIE40_RS03640 0.74 +0.4 720,184 - ABIE40_RS03640 0.75 -1.9 720,311 - +1.1 720,355 + -1.2 720,356 - +0.2 720,408 + ABIE40_RS03645 0.11 -0.7 720,408 + ABIE40_RS03645 0.11 -0.9 720,408 + ABIE40_RS03645 0.11 +0.2 720,409 - ABIE40_RS03645 0.12 +0.8 720,409 - ABIE40_RS03645 0.12 -1.5 720,409 - ABIE40_RS03645 0.12 -1.0 720,409 - ABIE40_RS03645 0.12 +0.2 720,409 - ABIE40_RS03645 0.12 +0.1 720,409 - ABIE40_RS03645 0.12 -0.5 720,409 - ABIE40_RS03645 0.12 +0.2 720,409 - ABIE40_RS03645 0.12 -0.5 720,409 - ABIE40_RS03645 0.12 -0.5 720,484 + ABIE40_RS03645 0.21 +1.2 720,484 + ABIE40_RS03645 0.21 -0.6 720,484 + ABIE40_RS03645 0.21 +0.1 720,484 + ABIE40_RS03645 0.21 +0.6 720,484 + ABIE40_RS03645 0.21 +1.6 720,484 + ABIE40_RS03645 0.21 -1.7 720,485 - ABIE40_RS03645 0.22 -0.1 720,485 - ABIE40_RS03645 0.22 -0.9 720,486 - ABIE40_RS03645 0.22 +1.2 720,492 - ABIE40_RS03645 0.22 -0.9 720,492 - ABIE40_RS03645 0.22 +0.9 720,492 - ABIE40_RS03645 0.22 -2.4 720,492 - ABIE40_RS03645 0.22 +0.1 720,492 - ABIE40_RS03645 0.22 -1.4 720,539 + ABIE40_RS03645 0.29 -0.7 720,540 - ABIE40_RS03645 0.29 -1.1 720,540 - ABIE40_RS03645 0.29 -0.0 720,540 - ABIE40_RS03645 0.29 +0.2 720,540 - ABIE40_RS03645 0.29 -0.1 720,566 + ABIE40_RS03645 0.32 -2.1 720,567 - ABIE40_RS03645 0.32 +0.1 720,665 - ABIE40_RS03645 0.45 +1.3 720,724 + ABIE40_RS03645 0.53 +0.5 720,828 - ABIE40_RS03645 0.67 -0.3
Or see this region's nucleotide sequence