Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS08005 and ABIE40_RS08010 are separated by 148 nucleotides ABIE40_RS08010 and ABIE40_RS08015 overlap by 14 nucleotides ABIE40_RS08015 and ABIE40_RS08020 are separated by 3 nucleotides ABIE40_RS08020 and ABIE40_RS08025 are separated by 31 nucleotides ABIE40_RS08025 and ABIE40_RS08030 are separated by 153 nucleotides
ABIE40_RS08005: ABIE40_RS08005 - dihydroxy-acid dehydratase, at 1,546,334 to 1,548,169
_RS08005
ABIE40_RS08010: ABIE40_RS08010 - type II toxin-antitoxin system PrlF family antitoxin, at 1,548,318 to 1,548,536
_RS08010
ABIE40_RS08015: ABIE40_RS08015 - type II toxin-antitoxin system PemK/MazF family toxin, at 1,548,523 to 1,548,861
_RS08015
ABIE40_RS08020: ABIE40_RS08020 - lysozyme inhibitor LprI family protein, at 1,548,865 to 1,549,209
_RS08020
ABIE40_RS08025: ABIE40_RS08025 - YkvA family protein, at 1,549,241 to 1,549,612
_RS08025
ABIE40_RS08030: ABIE40_RS08030 - sensor domain-containing diguanylate cyclase, at 1,549,766 to 1,551,016
_RS08030
Position (kb)
1548
1549
1550 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1548.106 kb on - strand at 1548.211 kb on - strand at 1548.211 kb on - strand at 1548.264 kb on - strand at 1548.411 kb on + strand, within ABIE40_RS08010 at 1548.411 kb on + strand, within ABIE40_RS08010 at 1548.412 kb on - strand, within ABIE40_RS08010 at 1548.412 kb on - strand, within ABIE40_RS08010 at 1548.412 kb on - strand, within ABIE40_RS08010 at 1548.413 kb on + strand, within ABIE40_RS08010 at 1548.413 kb on + strand, within ABIE40_RS08010 at 1548.413 kb on + strand, within ABIE40_RS08010 at 1548.414 kb on - strand, within ABIE40_RS08010 at 1548.522 kb on + strand at 1548.522 kb on + strand at 1548.522 kb on + strand at 1548.522 kb on + strand at 1548.522 kb on + strand at 1548.523 kb on - strand at 1548.523 kb on - strand at 1548.523 kb on - strand at 1548.551 kb on + strand at 1548.568 kb on + strand, within ABIE40_RS08015 at 1548.568 kb on + strand, within ABIE40_RS08015 at 1548.568 kb on + strand, within ABIE40_RS08015 at 1548.569 kb on - strand, within ABIE40_RS08015 at 1548.882 kb on + strand at 1548.882 kb on + strand at 1548.882 kb on + strand at 1548.882 kb on + strand at 1548.883 kb on - strand at 1548.888 kb on + strand at 1548.888 kb on + strand at 1548.888 kb on + strand at 1548.889 kb on - strand at 1548.889 kb on - strand at 1548.890 kb on + strand at 1548.890 kb on + strand at 1548.890 kb on + strand at 1548.890 kb on + strand at 1548.890 kb on + strand at 1548.891 kb on - strand at 1548.891 kb on - strand at 1548.891 kb on - strand at 1548.891 kb on - strand at 1548.891 kb on - strand at 1548.891 kb on - strand at 1548.891 kb on - strand at 1548.891 kb on - strand at 1548.891 kb on - strand at 1548.924 kb on - strand, within ABIE40_RS08020 at 1548.973 kb on - strand, within ABIE40_RS08020 at 1548.973 kb on - strand, within ABIE40_RS08020 at 1549.216 kb on + strand at 1549.216 kb on + strand at 1549.217 kb on - strand at 1549.220 kb on + strand at 1549.243 kb on - strand at 1549.244 kb on + strand at 1549.245 kb on - strand at 1549.365 kb on + strand, within ABIE40_RS08025 at 1549.482 kb on + strand, within ABIE40_RS08025 at 1549.482 kb on + strand, within ABIE40_RS08025 at 1549.515 kb on + strand, within ABIE40_RS08025 at 1549.515 kb on + strand, within ABIE40_RS08025 at 1549.516 kb on - strand, within ABIE40_RS08025 at 1549.698 kb on + strand at 1549.698 kb on + strand at 1549.699 kb on - strand at 1549.699 kb on - strand at 1549.701 kb on - strand at 1549.719 kb on + strand at 1549.719 kb on + strand at 1549.719 kb on + strand at 1549.719 kb on + strand at 1549.720 kb on - strand at 1549.720 kb on - strand at 1549.720 kb on - strand at 1549.720 kb on - strand at 1549.720 kb on - strand at 1549.720 kb on - strand at 1549.720 kb on - strand at 1549.720 kb on - strand at 1549.720 kb on - strand at 1549.721 kb on + strand at 1549.722 kb on - strand at 1549.736 kb on + strand at 1549.737 kb on - strand at 1549.739 kb on - strand at 1549.790 kb on - strand at 1550.011 kb on + strand, within ABIE40_RS08030 at 1550.011 kb on + strand, within ABIE40_RS08030 at 1550.011 kb on + strand, within ABIE40_RS08030 at 1550.011 kb on + strand, within ABIE40_RS08030 at 1550.012 kb on - strand, within ABIE40_RS08030 at 1550.012 kb on - strand, within ABIE40_RS08030 at 1550.012 kb on - strand, within ABIE40_RS08030 at 1550.012 kb on - strand, within ABIE40_RS08030 at 1550.024 kb on + strand, within ABIE40_RS08030 at 1550.024 kb on + strand, within ABIE40_RS08030 at 1550.126 kb on + strand, within ABIE40_RS08030 at 1550.126 kb on + strand, within ABIE40_RS08030 at 1550.126 kb on + strand, within ABIE40_RS08030 at 1550.127 kb on - strand, within ABIE40_RS08030 at 1550.127 kb on - strand, within ABIE40_RS08030 at 1550.127 kb on - strand, within ABIE40_RS08030 at 1550.161 kb on + strand, within ABIE40_RS08030 at 1550.161 kb on + strand, within ABIE40_RS08030 at 1550.161 kb on + strand, within ABIE40_RS08030 at 1550.162 kb on - strand, within ABIE40_RS08030 at 1550.162 kb on - strand, within ABIE40_RS08030 at 1550.162 kb on - strand, within ABIE40_RS08030 at 1550.162 kb on - strand, within ABIE40_RS08030 at 1550.162 kb on - strand, within ABIE40_RS08030
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 1,548,106 - +0.9 1,548,211 - +0.2 1,548,211 - -1.2 1,548,264 - -0.1 1,548,411 + ABIE40_RS08010 0.42 -1.5 1,548,411 + ABIE40_RS08010 0.42 -0.2 1,548,412 - ABIE40_RS08010 0.43 -0.6 1,548,412 - ABIE40_RS08010 0.43 -0.5 1,548,412 - ABIE40_RS08010 0.43 -0.1 1,548,413 + ABIE40_RS08010 0.43 -2.6 1,548,413 + ABIE40_RS08010 0.43 +1.1 1,548,413 + ABIE40_RS08010 0.43 +0.3 1,548,414 - ABIE40_RS08010 0.44 -1.1 1,548,522 + -0.8 1,548,522 + -0.9 1,548,522 + +0.4 1,548,522 + +1.9 1,548,522 + +1.3 1,548,523 - -0.7 1,548,523 - -0.3 1,548,523 - -1.3 1,548,551 + -0.5 1,548,568 + ABIE40_RS08015 0.13 +0.3 1,548,568 + ABIE40_RS08015 0.13 -2.4 1,548,568 + ABIE40_RS08015 0.13 -0.1 1,548,569 - ABIE40_RS08015 0.14 -2.3 1,548,882 + -1.1 1,548,882 + -1.5 1,548,882 + -2.4 1,548,882 + -0.5 1,548,883 - +0.7 1,548,888 + +1.2 1,548,888 + -1.0 1,548,888 + -0.4 1,548,889 - -0.6 1,548,889 - -1.1 1,548,890 + +1.1 1,548,890 + +0.7 1,548,890 + -0.4 1,548,890 + -0.8 1,548,890 + +0.2 1,548,891 - -0.9 1,548,891 - +0.3 1,548,891 - -2.8 1,548,891 - +0.5 1,548,891 - +0.6 1,548,891 - -0.2 1,548,891 - -1.2 1,548,891 - -0.4 1,548,891 - -0.4 1,548,924 - ABIE40_RS08020 0.17 -1.6 1,548,973 - ABIE40_RS08020 0.31 -0.8 1,548,973 - ABIE40_RS08020 0.31 -1.3 1,549,216 + +0.7 1,549,216 + +0.6 1,549,217 - -0.7 1,549,220 + -1.1 1,549,243 - +0.5 1,549,244 + +0.8 1,549,245 - -0.3 1,549,365 + ABIE40_RS08025 0.33 +0.1 1,549,482 + ABIE40_RS08025 0.65 -2.2 1,549,482 + ABIE40_RS08025 0.65 -1.7 1,549,515 + ABIE40_RS08025 0.74 -0.1 1,549,515 + ABIE40_RS08025 0.74 -0.1 1,549,516 - ABIE40_RS08025 0.74 -0.3 1,549,698 + -2.0 1,549,698 + -0.7 1,549,699 - +0.1 1,549,699 - -1.0 1,549,701 - -1.7 1,549,719 + -0.8 1,549,719 + -2.1 1,549,719 + +0.2 1,549,719 + +1.0 1,549,720 - -1.6 1,549,720 - -0.8 1,549,720 - -0.4 1,549,720 - +0.3 1,549,720 - +0.0 1,549,720 - -0.2 1,549,720 - -0.9 1,549,720 - -0.6 1,549,720 - -0.5 1,549,721 + +0.6 1,549,722 - -1.5 1,549,736 + -1.0 1,549,737 - -0.4 1,549,739 - -1.1 1,549,790 - -1.9 1,550,011 + ABIE40_RS08030 0.20 -0.1 1,550,011 + ABIE40_RS08030 0.20 -0.4 1,550,011 + ABIE40_RS08030 0.20 -0.2 1,550,011 + ABIE40_RS08030 0.20 +0.4 1,550,012 - ABIE40_RS08030 0.20 -0.5 1,550,012 - ABIE40_RS08030 0.20 -1.4 1,550,012 - ABIE40_RS08030 0.20 +0.2 1,550,012 - ABIE40_RS08030 0.20 +0.3 1,550,024 + ABIE40_RS08030 0.21 +1.0 1,550,024 + ABIE40_RS08030 0.21 -0.1 1,550,126 + ABIE40_RS08030 0.29 -0.1 1,550,126 + ABIE40_RS08030 0.29 -1.9 1,550,126 + ABIE40_RS08030 0.29 -0.8 1,550,127 - ABIE40_RS08030 0.29 -1.0 1,550,127 - ABIE40_RS08030 0.29 +2.1 1,550,127 - ABIE40_RS08030 0.29 +0.3 1,550,161 + ABIE40_RS08030 0.32 +0.5 1,550,161 + ABIE40_RS08030 0.32 +1.1 1,550,161 + ABIE40_RS08030 0.32 +1.9 1,550,162 - ABIE40_RS08030 0.32 -0.8 1,550,162 - ABIE40_RS08030 0.32 -0.8 1,550,162 - ABIE40_RS08030 0.32 +1.9 1,550,162 - ABIE40_RS08030 0.32 +0.8 1,550,162 - ABIE40_RS08030 0.32 +0.1
Or see this region's nucleotide sequence