Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS23115 and ABIE40_RS23120 are separated by 312 nucleotides ABIE40_RS23120 and ABIE40_RS23125 are separated by 198 nucleotides ABIE40_RS23125 and ABIE40_RS23130 are separated by 59 nucleotides ABIE40_RS23130 and ABIE40_RS23135 are separated by 27 nucleotides
ABIE40_RS23115: ABIE40_RS23115 - methionine ABC transporter ATP-binding protein, at 769,411 to 770,538
_RS23115
ABIE40_RS23120: ABIE40_RS23120 - RidA family protein, at 770,851 to 771,198
_RS23120
ABIE40_RS23125: ABIE40_RS23125 - glycine betaine ABC transporter substrate-binding protein, at 771,397 to 772,404
_RS23125
ABIE40_RS23130: ABIE40_RS23130 - ABC transporter ATP-binding protein, at 772,464 to 773,252
_RS23130
ABIE40_RS23135: ABIE40_RS23135 - ABC transporter permease subunit, at 773,280 to 774,119
_RS23135
Position (kb)
771
772
773 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 770.541 kb on + strand at 770.541 kb on + strand at 770.542 kb on - strand at 770.542 kb on - strand at 770.542 kb on - strand at 770.579 kb on + strand at 770.579 kb on + strand at 770.579 kb on + strand at 770.580 kb on - strand at 770.580 kb on - strand at 770.580 kb on - strand at 770.600 kb on + strand at 770.601 kb on - strand at 770.665 kb on + strand at 770.666 kb on - strand at 770.666 kb on - strand at 770.709 kb on + strand at 770.709 kb on + strand at 770.709 kb on + strand at 770.709 kb on + strand at 770.709 kb on + strand at 770.709 kb on + strand at 770.709 kb on + strand at 770.709 kb on + strand at 770.709 kb on + strand at 770.710 kb on - strand at 770.710 kb on - strand at 770.710 kb on - strand at 770.710 kb on - strand at 770.710 kb on - strand at 770.710 kb on - strand at 770.710 kb on - strand at 770.710 kb on - strand at 770.831 kb on - strand at 770.831 kb on - strand at 770.834 kb on + strand at 770.835 kb on - strand at 770.978 kb on + strand, within ABIE40_RS23120 at 770.979 kb on - strand, within ABIE40_RS23120 at 771.049 kb on + strand, within ABIE40_RS23120 at 771.049 kb on + strand, within ABIE40_RS23120 at 771.049 kb on + strand, within ABIE40_RS23120 at 771.049 kb on + strand, within ABIE40_RS23120 at 771.050 kb on - strand, within ABIE40_RS23120 at 771.050 kb on - strand, within ABIE40_RS23120 at 771.050 kb on - strand, within ABIE40_RS23120 at 771.050 kb on - strand, within ABIE40_RS23120 at 771.050 kb on - strand, within ABIE40_RS23120 at 771.163 kb on - strand, within ABIE40_RS23120 at 771.163 kb on - strand, within ABIE40_RS23120 at 771.163 kb on - strand, within ABIE40_RS23120 at 771.163 kb on - strand, within ABIE40_RS23120 at 771.163 kb on - strand, within ABIE40_RS23120 at 771.258 kb on - strand at 771.258 kb on - strand at 771.322 kb on - strand at 771.403 kb on - strand at 771.403 kb on - strand at 771.652 kb on + strand, within ABIE40_RS23125 at 771.694 kb on + strand, within ABIE40_RS23125 at 771.694 kb on + strand, within ABIE40_RS23125 at 771.694 kb on + strand, within ABIE40_RS23125 at 771.694 kb on + strand, within ABIE40_RS23125 at 771.695 kb on - strand, within ABIE40_RS23125 at 771.695 kb on - strand, within ABIE40_RS23125 at 771.695 kb on - strand, within ABIE40_RS23125 at 771.695 kb on - strand, within ABIE40_RS23125 at 771.695 kb on - strand, within ABIE40_RS23125 at 771.734 kb on + strand, within ABIE40_RS23125 at 771.735 kb on - strand, within ABIE40_RS23125 at 771.809 kb on - strand, within ABIE40_RS23125 at 771.824 kb on - strand, within ABIE40_RS23125 at 772.075 kb on + strand, within ABIE40_RS23125 at 772.075 kb on + strand, within ABIE40_RS23125 at 772.075 kb on + strand, within ABIE40_RS23125 at 772.076 kb on - strand, within ABIE40_RS23125 at 772.076 kb on - strand, within ABIE40_RS23125 at 772.076 kb on - strand, within ABIE40_RS23125 at 772.076 kb on - strand, within ABIE40_RS23125 at 772.096 kb on + strand, within ABIE40_RS23125 at 772.096 kb on + strand, within ABIE40_RS23125 at 772.096 kb on + strand, within ABIE40_RS23125 at 772.096 kb on + strand, within ABIE40_RS23125 at 772.096 kb on + strand, within ABIE40_RS23125 at 772.096 kb on + strand, within ABIE40_RS23125 at 772.096 kb on + strand, within ABIE40_RS23125 at 772.096 kb on + strand, within ABIE40_RS23125 at 772.096 kb on + strand, within ABIE40_RS23125 at 772.096 kb on + strand, within ABIE40_RS23125 at 772.097 kb on - strand, within ABIE40_RS23125 at 772.097 kb on - strand, within ABIE40_RS23125 at 772.097 kb on - strand, within ABIE40_RS23125 at 772.097 kb on - strand, within ABIE40_RS23125 at 772.097 kb on - strand, within ABIE40_RS23125 at 772.099 kb on + strand, within ABIE40_RS23125 at 772.099 kb on + strand, within ABIE40_RS23125 at 772.099 kb on + strand, within ABIE40_RS23125 at 772.099 kb on + strand, within ABIE40_RS23125 at 772.099 kb on + strand, within ABIE40_RS23125 at 772.100 kb on - strand, within ABIE40_RS23125 at 772.100 kb on - strand, within ABIE40_RS23125 at 772.100 kb on - strand, within ABIE40_RS23125 at 772.180 kb on - strand, within ABIE40_RS23125 at 772.241 kb on + strand, within ABIE40_RS23125 at 772.241 kb on + strand, within ABIE40_RS23125 at 772.347 kb on + strand at 772.348 kb on - strand at 772.351 kb on + strand at 772.351 kb on + strand at 772.351 kb on + strand at 772.351 kb on + strand at 772.351 kb on + strand at 772.351 kb on + strand at 772.351 kb on + strand at 772.352 kb on - strand at 772.352 kb on - strand at 772.352 kb on - strand at 772.352 kb on - strand at 772.449 kb on + strand at 772.449 kb on + strand at 772.450 kb on - strand at 772.450 kb on - strand at 772.450 kb on - strand at 772.716 kb on - strand, within ABIE40_RS23130 at 772.777 kb on + strand, within ABIE40_RS23130 at 772.777 kb on + strand, within ABIE40_RS23130 at 772.777 kb on + strand, within ABIE40_RS23130 at 772.777 kb on + strand, within ABIE40_RS23130 at 772.778 kb on - strand, within ABIE40_RS23130 at 772.778 kb on - strand, within ABIE40_RS23130 at 772.778 kb on - strand, within ABIE40_RS23130 at 772.781 kb on + strand, within ABIE40_RS23130 at 772.782 kb on - strand, within ABIE40_RS23130 at 772.782 kb on - strand, within ABIE40_RS23130 at 772.782 kb on - strand, within ABIE40_RS23130 at 772.782 kb on - strand, within ABIE40_RS23130 at 772.858 kb on - strand, within ABIE40_RS23130 at 772.858 kb on - strand, within ABIE40_RS23130 at 773.028 kb on + strand, within ABIE40_RS23130 at 773.033 kb on + strand, within ABIE40_RS23130 at 773.033 kb on + strand, within ABIE40_RS23130 at 773.034 kb on - strand, within ABIE40_RS23130 at 773.116 kb on - strand, within ABIE40_RS23130 at 773.280 kb on - strand at 773.280 kb on - strand at 773.287 kb on + strand at 773.288 kb on - strand at 773.404 kb on + strand, within ABIE40_RS23135 at 773.404 kb on + strand, within ABIE40_RS23135 at 773.404 kb on + strand, within ABIE40_RS23135 at 773.404 kb on + strand, within ABIE40_RS23135 at 773.404 kb on + strand, within ABIE40_RS23135
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 770,541 + -1.3 770,541 + -0.6 770,542 - +0.1 770,542 - -0.9 770,542 - -1.9 770,579 + +0.5 770,579 + -0.8 770,579 + -1.9 770,580 - -1.8 770,580 - +1.1 770,580 - -0.7 770,600 + -1.6 770,601 - +1.8 770,665 + +0.1 770,666 - -0.5 770,666 - +0.3 770,709 + +0.2 770,709 + -0.9 770,709 + -2.0 770,709 + -0.4 770,709 + +1.4 770,709 + +0.5 770,709 + +0.5 770,709 + -0.1 770,709 + -0.1 770,710 - +0.1 770,710 - -0.1 770,710 - +0.2 770,710 - -1.8 770,710 - +1.2 770,710 - +1.1 770,710 - -0.2 770,710 - -0.5 770,831 - -0.1 770,831 - +0.5 770,834 + -0.7 770,835 - -0.5 770,978 + ABIE40_RS23120 0.36 +1.0 770,979 - ABIE40_RS23120 0.37 +2.1 771,049 + ABIE40_RS23120 0.57 +0.4 771,049 + ABIE40_RS23120 0.57 -0.5 771,049 + ABIE40_RS23120 0.57 -0.0 771,049 + ABIE40_RS23120 0.57 +0.4 771,050 - ABIE40_RS23120 0.57 -1.4 771,050 - ABIE40_RS23120 0.57 +1.7 771,050 - ABIE40_RS23120 0.57 +1.5 771,050 - ABIE40_RS23120 0.57 -0.2 771,050 - ABIE40_RS23120 0.57 +1.5 771,163 - ABIE40_RS23120 0.90 +0.7 771,163 - ABIE40_RS23120 0.90 +0.3 771,163 - ABIE40_RS23120 0.90 +1.2 771,163 - ABIE40_RS23120 0.90 -1.5 771,163 - ABIE40_RS23120 0.90 +3.2 771,258 - +0.2 771,258 - +3.0 771,322 - -0.9 771,403 - -0.7 771,403 - +0.7 771,652 + ABIE40_RS23125 0.25 -0.9 771,694 + ABIE40_RS23125 0.29 -0.6 771,694 + ABIE40_RS23125 0.29 -0.6 771,694 + ABIE40_RS23125 0.29 -1.1 771,694 + ABIE40_RS23125 0.29 +0.6 771,695 - ABIE40_RS23125 0.30 +0.6 771,695 - ABIE40_RS23125 0.30 -0.0 771,695 - ABIE40_RS23125 0.30 -1.1 771,695 - ABIE40_RS23125 0.30 +0.6 771,695 - ABIE40_RS23125 0.30 +0.6 771,734 + ABIE40_RS23125 0.33 +0.1 771,735 - ABIE40_RS23125 0.34 -0.7 771,809 - ABIE40_RS23125 0.41 -1.8 771,824 - ABIE40_RS23125 0.42 +2.1 772,075 + ABIE40_RS23125 0.67 +2.1 772,075 + ABIE40_RS23125 0.67 +0.9 772,075 + ABIE40_RS23125 0.67 +1.8 772,076 - ABIE40_RS23125 0.67 +0.2 772,076 - ABIE40_RS23125 0.67 -0.9 772,076 - ABIE40_RS23125 0.67 +1.1 772,076 - ABIE40_RS23125 0.67 +0.9 772,096 + ABIE40_RS23125 0.69 +0.1 772,096 + ABIE40_RS23125 0.69 +0.5 772,096 + ABIE40_RS23125 0.69 -0.1 772,096 + ABIE40_RS23125 0.69 -1.5 772,096 + ABIE40_RS23125 0.69 +1.4 772,096 + ABIE40_RS23125 0.69 -0.8 772,096 + ABIE40_RS23125 0.69 -0.4 772,096 + ABIE40_RS23125 0.69 +0.1 772,096 + ABIE40_RS23125 0.69 -0.8 772,096 + ABIE40_RS23125 0.69 -0.3 772,097 - ABIE40_RS23125 0.69 +0.1 772,097 - ABIE40_RS23125 0.69 -2.7 772,097 - ABIE40_RS23125 0.69 +0.7 772,097 - ABIE40_RS23125 0.69 +1.1 772,097 - ABIE40_RS23125 0.69 -1.4 772,099 + ABIE40_RS23125 0.70 +0.8 772,099 + ABIE40_RS23125 0.70 +1.0 772,099 + ABIE40_RS23125 0.70 -0.0 772,099 + ABIE40_RS23125 0.70 -0.3 772,099 + ABIE40_RS23125 0.70 -0.6 772,100 - ABIE40_RS23125 0.70 +0.5 772,100 - ABIE40_RS23125 0.70 -1.2 772,100 - ABIE40_RS23125 0.70 +1.3 772,180 - ABIE40_RS23125 0.78 -0.3 772,241 + ABIE40_RS23125 0.84 -0.3 772,241 + ABIE40_RS23125 0.84 +0.1 772,347 + +0.7 772,348 - +0.8 772,351 + +1.1 772,351 + -0.5 772,351 + +0.5 772,351 + -0.4 772,351 + +0.8 772,351 + +0.9 772,351 + +0.2 772,352 - -0.5 772,352 - +0.0 772,352 - -0.5 772,352 - -0.1 772,449 + -0.2 772,449 + -0.2 772,450 - +0.9 772,450 - +0.8 772,450 - +0.9 772,716 - ABIE40_RS23130 0.32 +1.8 772,777 + ABIE40_RS23130 0.40 -0.8 772,777 + ABIE40_RS23130 0.40 +0.0 772,777 + ABIE40_RS23130 0.40 -0.1 772,777 + ABIE40_RS23130 0.40 -0.5 772,778 - ABIE40_RS23130 0.40 -0.2 772,778 - ABIE40_RS23130 0.40 +0.9 772,778 - ABIE40_RS23130 0.40 +0.7 772,781 + ABIE40_RS23130 0.40 +0.3 772,782 - ABIE40_RS23130 0.40 -1.3 772,782 - ABIE40_RS23130 0.40 +0.1 772,782 - ABIE40_RS23130 0.40 +0.1 772,782 - ABIE40_RS23130 0.40 -0.7 772,858 - ABIE40_RS23130 0.50 -0.2 772,858 - ABIE40_RS23130 0.50 -0.9 773,028 + ABIE40_RS23130 0.71 -0.1 773,033 + ABIE40_RS23130 0.72 -0.8 773,033 + ABIE40_RS23130 0.72 +0.6 773,034 - ABIE40_RS23130 0.72 -1.8 773,116 - ABIE40_RS23130 0.83 -2.1 773,280 - -1.2 773,280 - +0.2 773,287 + +1.9 773,288 - +0.4 773,404 + ABIE40_RS23135 0.15 -2.0 773,404 + ABIE40_RS23135 0.15 +2.1 773,404 + ABIE40_RS23135 0.15 +0.6 773,404 + ABIE40_RS23135 0.15 +0.5 773,404 + ABIE40_RS23135 0.15 +0.2
Or see this region's nucleotide sequence