Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS22545 and ABIE40_RS22550 are separated by 124 nucleotides ABIE40_RS22550 and ABIE40_RS22555 are separated by 116 nucleotides
ABIE40_RS22545: ABIE40_RS22545 - alpha-glucosidase/alpha-galactosidase, at 645,264 to 646,730
_RS22545
ABIE40_RS22550: ABIE40_RS22550 - ABC transporter substrate-binding protein, at 646,855 to 648,942
_RS22550
ABIE40_RS22555: ABIE40_RS22555 - ABC transporter permease, at 649,059 to 650,066
_RS22555
Position (kb)
646
647
648
649 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 646.003 kb on - strand, within ABIE40_RS22545 at 646.035 kb on + strand, within ABIE40_RS22545 at 646.035 kb on + strand, within ABIE40_RS22545 at 646.100 kb on - strand, within ABIE40_RS22545 at 646.100 kb on - strand, within ABIE40_RS22545 at 646.119 kb on + strand, within ABIE40_RS22545 at 646.235 kb on + strand, within ABIE40_RS22545 at 646.235 kb on + strand, within ABIE40_RS22545 at 646.235 kb on + strand, within ABIE40_RS22545 at 646.236 kb on - strand, within ABIE40_RS22545 at 646.236 kb on - strand, within ABIE40_RS22545 at 646.587 kb on + strand at 646.588 kb on - strand at 646.601 kb on - strand at 646.990 kb on + strand at 646.990 kb on + strand at 646.990 kb on + strand at 646.991 kb on - strand at 646.991 kb on - strand at 646.991 kb on - strand at 646.991 kb on - strand at 646.991 kb on - strand at 646.991 kb on - strand at 646.991 kb on - strand at 646.991 kb on - strand at 646.991 kb on - strand at 647.035 kb on + strand at 647.035 kb on + strand at 647.036 kb on - strand at 647.037 kb on + strand at 647.038 kb on - strand at 647.161 kb on + strand, within ABIE40_RS22550 at 647.161 kb on + strand, within ABIE40_RS22550 at 647.162 kb on - strand, within ABIE40_RS22550 at 647.185 kb on - strand, within ABIE40_RS22550 at 647.212 kb on + strand, within ABIE40_RS22550 at 647.212 kb on + strand, within ABIE40_RS22550 at 647.212 kb on + strand, within ABIE40_RS22550 at 647.330 kb on + strand, within ABIE40_RS22550 at 647.331 kb on - strand, within ABIE40_RS22550 at 647.331 kb on - strand, within ABIE40_RS22550 at 647.440 kb on + strand, within ABIE40_RS22550 at 647.542 kb on + strand, within ABIE40_RS22550 at 647.542 kb on + strand, within ABIE40_RS22550 at 647.543 kb on - strand, within ABIE40_RS22550 at 647.543 kb on - strand, within ABIE40_RS22550 at 647.543 kb on - strand, within ABIE40_RS22550 at 647.543 kb on - strand, within ABIE40_RS22550 at 647.543 kb on - strand, within ABIE40_RS22550 at 647.584 kb on + strand, within ABIE40_RS22550 at 647.584 kb on + strand, within ABIE40_RS22550 at 647.584 kb on + strand, within ABIE40_RS22550 at 647.584 kb on + strand, within ABIE40_RS22550 at 647.585 kb on - strand, within ABIE40_RS22550 at 647.585 kb on - strand, within ABIE40_RS22550 at 647.585 kb on - strand, within ABIE40_RS22550 at 647.585 kb on - strand, within ABIE40_RS22550 at 647.585 kb on - strand, within ABIE40_RS22550 at 647.585 kb on - strand, within ABIE40_RS22550 at 647.585 kb on - strand, within ABIE40_RS22550 at 647.591 kb on - strand, within ABIE40_RS22550 at 647.592 kb on + strand, within ABIE40_RS22550 at 647.593 kb on - strand, within ABIE40_RS22550 at 647.593 kb on - strand, within ABIE40_RS22550 at 647.593 kb on - strand, within ABIE40_RS22550 at 647.593 kb on - strand, within ABIE40_RS22550 at 647.603 kb on - strand, within ABIE40_RS22550 at 647.631 kb on + strand, within ABIE40_RS22550 at 647.632 kb on - strand, within ABIE40_RS22550 at 647.656 kb on + strand, within ABIE40_RS22550 at 647.657 kb on - strand, within ABIE40_RS22550 at 647.671 kb on + strand, within ABIE40_RS22550 at 647.671 kb on + strand, within ABIE40_RS22550 at 647.671 kb on + strand, within ABIE40_RS22550 at 647.672 kb on - strand, within ABIE40_RS22550 at 647.746 kb on + strand, within ABIE40_RS22550 at 647.759 kb on + strand, within ABIE40_RS22550 at 647.785 kb on + strand, within ABIE40_RS22550 at 647.788 kb on + strand, within ABIE40_RS22550 at 647.788 kb on + strand, within ABIE40_RS22550 at 647.824 kb on + strand, within ABIE40_RS22550 at 647.842 kb on + strand, within ABIE40_RS22550 at 647.843 kb on - strand, within ABIE40_RS22550 at 648.002 kb on - strand, within ABIE40_RS22550 at 648.002 kb on - strand, within ABIE40_RS22550 at 648.202 kb on + strand, within ABIE40_RS22550 at 648.202 kb on + strand, within ABIE40_RS22550 at 648.203 kb on - strand, within ABIE40_RS22550 at 648.203 kb on - strand, within ABIE40_RS22550 at 648.223 kb on + strand, within ABIE40_RS22550 at 648.223 kb on + strand, within ABIE40_RS22550 at 648.223 kb on + strand, within ABIE40_RS22550 at 648.223 kb on + strand, within ABIE40_RS22550 at 648.224 kb on - strand, within ABIE40_RS22550 at 648.224 kb on - strand, within ABIE40_RS22550 at 648.224 kb on - strand, within ABIE40_RS22550 at 648.226 kb on + strand, within ABIE40_RS22550 at 648.226 kb on + strand, within ABIE40_RS22550 at 648.465 kb on + strand, within ABIE40_RS22550 at 648.466 kb on - strand, within ABIE40_RS22550 at 648.475 kb on + strand, within ABIE40_RS22550 at 648.476 kb on - strand, within ABIE40_RS22550 at 648.476 kb on - strand, within ABIE40_RS22550 at 648.623 kb on - strand, within ABIE40_RS22550 at 648.703 kb on + strand, within ABIE40_RS22550 at 648.751 kb on + strand at 648.751 kb on + strand at 648.788 kb on - strand at 648.802 kb on - strand at 648.802 kb on - strand at 648.883 kb on + strand at 648.940 kb on + strand at 648.940 kb on + strand at 648.940 kb on + strand at 648.941 kb on - strand at 648.941 kb on - strand at 648.941 kb on - strand at 648.941 kb on - strand at 648.999 kb on + strand at 648.999 kb on + strand at 648.999 kb on + strand at 649.000 kb on - strand at 649.000 kb on - strand at 649.020 kb on + strand at 649.158 kb on + strand at 649.159 kb on - strand at 649.167 kb on + strand, within ABIE40_RS22555 at 649.168 kb on - strand, within ABIE40_RS22555 at 649.168 kb on - strand, within ABIE40_RS22555 at 649.259 kb on + strand, within ABIE40_RS22555 at 649.260 kb on - strand, within ABIE40_RS22555 at 649.279 kb on - strand, within ABIE40_RS22555 at 649.335 kb on + strand, within ABIE40_RS22555 at 649.335 kb on + strand, within ABIE40_RS22555 at 649.336 kb on - strand, within ABIE40_RS22555 at 649.336 kb on - strand, within ABIE40_RS22555 at 649.430 kb on - strand, within ABIE40_RS22555 at 649.461 kb on + strand, within ABIE40_RS22555 at 649.461 kb on + strand, within ABIE40_RS22555 at 649.461 kb on + strand, within ABIE40_RS22555 at 649.461 kb on + strand, within ABIE40_RS22555 at 649.461 kb on + strand, within ABIE40_RS22555 at 649.462 kb on - strand, within ABIE40_RS22555 at 649.545 kb on + strand, within ABIE40_RS22555 at 649.545 kb on + strand, within ABIE40_RS22555 at 649.545 kb on + strand, within ABIE40_RS22555 at 649.546 kb on - strand, within ABIE40_RS22555 at 649.546 kb on - strand, within ABIE40_RS22555 at 649.603 kb on - strand, within ABIE40_RS22555 at 649.695 kb on + strand, within ABIE40_RS22555 at 649.695 kb on + strand, within ABIE40_RS22555 at 649.696 kb on - strand, within ABIE40_RS22555 at 649.746 kb on + strand, within ABIE40_RS22555 at 649.746 kb on + strand, within ABIE40_RS22555 at 649.747 kb on - strand, within ABIE40_RS22555 at 649.747 kb on - strand, within ABIE40_RS22555 at 649.747 kb on - strand, within ABIE40_RS22555 at 649.747 kb on - strand, within ABIE40_RS22555 at 649.836 kb on + strand, within ABIE40_RS22555 at 649.836 kb on + strand, within ABIE40_RS22555 at 649.836 kb on + strand, within ABIE40_RS22555 at 649.837 kb on - strand, within ABIE40_RS22555 at 649.837 kb on - strand, within ABIE40_RS22555 at 649.837 kb on - strand, within ABIE40_RS22555 at 649.837 kb on - strand, within ABIE40_RS22555 at 649.857 kb on + strand, within ABIE40_RS22555 at 649.857 kb on + strand, within ABIE40_RS22555 at 649.858 kb on - strand, within ABIE40_RS22555
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 646,003 - ABIE40_RS22545 0.50 -0.9 646,035 + ABIE40_RS22545 0.53 -0.5 646,035 + ABIE40_RS22545 0.53 +0.7 646,100 - ABIE40_RS22545 0.57 -0.3 646,100 - ABIE40_RS22545 0.57 -1.2 646,119 + ABIE40_RS22545 0.58 +1.0 646,235 + ABIE40_RS22545 0.66 +1.1 646,235 + ABIE40_RS22545 0.66 -1.2 646,235 + ABIE40_RS22545 0.66 +0.3 646,236 - ABIE40_RS22545 0.66 -1.6 646,236 - ABIE40_RS22545 0.66 +0.1 646,587 + +1.0 646,588 - +0.6 646,601 - +2.0 646,990 + -0.1 646,990 + +2.0 646,990 + +0.0 646,991 - -0.4 646,991 - +2.0 646,991 - +2.0 646,991 - -1.1 646,991 - -1.1 646,991 - -0.3 646,991 - -0.7 646,991 - -1.7 646,991 - +0.1 647,035 + -0.9 647,035 + -0.1 647,036 - +0.3 647,037 + -0.1 647,038 - +3.0 647,161 + ABIE40_RS22550 0.15 -0.3 647,161 + ABIE40_RS22550 0.15 -0.2 647,162 - ABIE40_RS22550 0.15 -0.7 647,185 - ABIE40_RS22550 0.16 -4.0 647,212 + ABIE40_RS22550 0.17 -0.8 647,212 + ABIE40_RS22550 0.17 -0.9 647,212 + ABIE40_RS22550 0.17 -0.9 647,330 + ABIE40_RS22550 0.23 +0.4 647,331 - ABIE40_RS22550 0.23 -0.5 647,331 - ABIE40_RS22550 0.23 +0.8 647,440 + ABIE40_RS22550 0.28 -0.3 647,542 + ABIE40_RS22550 0.33 -2.7 647,542 + ABIE40_RS22550 0.33 +0.4 647,543 - ABIE40_RS22550 0.33 +0.2 647,543 - ABIE40_RS22550 0.33 +1.0 647,543 - ABIE40_RS22550 0.33 -0.3 647,543 - ABIE40_RS22550 0.33 -0.3 647,543 - ABIE40_RS22550 0.33 -0.8 647,584 + ABIE40_RS22550 0.35 +0.1 647,584 + ABIE40_RS22550 0.35 -1.1 647,584 + ABIE40_RS22550 0.35 +0.4 647,584 + ABIE40_RS22550 0.35 -2.6 647,585 - ABIE40_RS22550 0.35 +0.2 647,585 - ABIE40_RS22550 0.35 -1.0 647,585 - ABIE40_RS22550 0.35 -0.7 647,585 - ABIE40_RS22550 0.35 -0.5 647,585 - ABIE40_RS22550 0.35 -2.2 647,585 - ABIE40_RS22550 0.35 -0.3 647,585 - ABIE40_RS22550 0.35 +1.0 647,591 - ABIE40_RS22550 0.35 -1.2 647,592 + ABIE40_RS22550 0.35 +0.2 647,593 - ABIE40_RS22550 0.35 -0.4 647,593 - ABIE40_RS22550 0.35 +0.4 647,593 - ABIE40_RS22550 0.35 +0.4 647,593 - ABIE40_RS22550 0.35 -0.4 647,603 - ABIE40_RS22550 0.36 -2.6 647,631 + ABIE40_RS22550 0.37 +1.4 647,632 - ABIE40_RS22550 0.37 -1.3 647,656 + ABIE40_RS22550 0.38 +0.3 647,657 - ABIE40_RS22550 0.38 -1.6 647,671 + ABIE40_RS22550 0.39 -1.2 647,671 + ABIE40_RS22550 0.39 -0.9 647,671 + ABIE40_RS22550 0.39 -1.2 647,672 - ABIE40_RS22550 0.39 -1.6 647,746 + ABIE40_RS22550 0.43 +0.7 647,759 + ABIE40_RS22550 0.43 -0.9 647,785 + ABIE40_RS22550 0.45 -0.1 647,788 + ABIE40_RS22550 0.45 -1.0 647,788 + ABIE40_RS22550 0.45 -0.4 647,824 + ABIE40_RS22550 0.46 +0.0 647,842 + ABIE40_RS22550 0.47 +0.8 647,843 - ABIE40_RS22550 0.47 +0.3 648,002 - ABIE40_RS22550 0.55 -2.3 648,002 - ABIE40_RS22550 0.55 +1.8 648,202 + ABIE40_RS22550 0.65 -2.1 648,202 + ABIE40_RS22550 0.65 +0.0 648,203 - ABIE40_RS22550 0.65 +0.2 648,203 - ABIE40_RS22550 0.65 -2.1 648,223 + ABIE40_RS22550 0.66 -0.5 648,223 + ABIE40_RS22550 0.66 -0.3 648,223 + ABIE40_RS22550 0.66 +1.1 648,223 + ABIE40_RS22550 0.66 -0.7 648,224 - ABIE40_RS22550 0.66 -0.3 648,224 - ABIE40_RS22550 0.66 +0.6 648,224 - ABIE40_RS22550 0.66 -0.2 648,226 + ABIE40_RS22550 0.66 +0.3 648,226 + ABIE40_RS22550 0.66 +0.4 648,465 + ABIE40_RS22550 0.77 -0.0 648,466 - ABIE40_RS22550 0.77 +0.2 648,475 + ABIE40_RS22550 0.78 +0.8 648,476 - ABIE40_RS22550 0.78 -0.4 648,476 - ABIE40_RS22550 0.78 -0.7 648,623 - ABIE40_RS22550 0.85 +0.0 648,703 + ABIE40_RS22550 0.89 -0.1 648,751 + +0.7 648,751 + -0.1 648,788 - -0.1 648,802 - +0.1 648,802 - +1.0 648,883 + -0.9 648,940 + +1.2 648,940 + -1.2 648,940 + +1.6 648,941 - -0.2 648,941 - -0.9 648,941 - +0.1 648,941 - +0.7 648,999 + +1.3 648,999 + -1.4 648,999 + -0.4 649,000 - +1.7 649,000 - -1.1 649,020 + -0.2 649,158 + -1.7 649,159 - -0.4 649,167 + ABIE40_RS22555 0.11 -0.5 649,168 - ABIE40_RS22555 0.11 +1.1 649,168 - ABIE40_RS22555 0.11 +0.3 649,259 + ABIE40_RS22555 0.20 +1.2 649,260 - ABIE40_RS22555 0.20 +0.7 649,279 - ABIE40_RS22555 0.22 -0.0 649,335 + ABIE40_RS22555 0.27 -0.3 649,335 + ABIE40_RS22555 0.27 +1.7 649,336 - ABIE40_RS22555 0.27 +0.2 649,336 - ABIE40_RS22555 0.27 -0.2 649,430 - ABIE40_RS22555 0.37 -0.3 649,461 + ABIE40_RS22555 0.40 -1.6 649,461 + ABIE40_RS22555 0.40 -0.3 649,461 + ABIE40_RS22555 0.40 -0.4 649,461 + ABIE40_RS22555 0.40 +0.5 649,461 + ABIE40_RS22555 0.40 -0.9 649,462 - ABIE40_RS22555 0.40 +0.0 649,545 + ABIE40_RS22555 0.48 -0.4 649,545 + ABIE40_RS22555 0.48 -1.7 649,545 + ABIE40_RS22555 0.48 -1.2 649,546 - ABIE40_RS22555 0.48 +0.7 649,546 - ABIE40_RS22555 0.48 +0.0 649,603 - ABIE40_RS22555 0.54 +0.5 649,695 + ABIE40_RS22555 0.63 -1.2 649,695 + ABIE40_RS22555 0.63 -0.6 649,696 - ABIE40_RS22555 0.63 +0.8 649,746 + ABIE40_RS22555 0.68 +2.2 649,746 + ABIE40_RS22555 0.68 +0.4 649,747 - ABIE40_RS22555 0.68 +2.0 649,747 - ABIE40_RS22555 0.68 -1.1 649,747 - ABIE40_RS22555 0.68 +0.1 649,747 - ABIE40_RS22555 0.68 +2.0 649,836 + ABIE40_RS22555 0.77 +0.2 649,836 + ABIE40_RS22555 0.77 +2.7 649,836 + ABIE40_RS22555 0.77 -1.3 649,837 - ABIE40_RS22555 0.77 +0.4 649,837 - ABIE40_RS22555 0.77 +0.4 649,837 - ABIE40_RS22555 0.77 +0.4 649,837 - ABIE40_RS22555 0.77 -2.6 649,857 + ABIE40_RS22555 0.79 +2.0 649,857 + ABIE40_RS22555 0.79 -0.5 649,858 - ABIE40_RS22555 0.79 -0.1
Or see this region's nucleotide sequence