Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS20210 and ABIE40_RS20215 are separated by 151 nucleotides ABIE40_RS20215 and ABIE40_RS20220 are separated by 29 nucleotides
ABIE40_RS20210: ABIE40_RS20210 - MFS transporter, at 143,222 to 144,397
_RS20210
ABIE40_RS20215: ABIE40_RS20215 - glutamine cyclotransferase, at 144,549 to 145,184
_RS20215
ABIE40_RS20220: ABIE40_RS20220 - helix-turn-helix domain-containing protein, at 145,214 to 146,434
_RS20220
Position (kb) 144
145
146 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 143.598 kb on - strand, within ABIE40_RS20210 at 143.598 kb on - strand, within ABIE40_RS20210 at 143.630 kb on + strand, within ABIE40_RS20210 at 143.790 kb on - strand, within ABIE40_RS20210 at 143.790 kb on - strand, within ABIE40_RS20210 at 144.327 kb on - strand at 144.475 kb on + strand at 144.771 kb on + strand, within ABIE40_RS20215 at 144.985 kb on + strand, within ABIE40_RS20215 at 144.985 kb on + strand, within ABIE40_RS20215 at 144.985 kb on + strand, within ABIE40_RS20215 at 145.150 kb on + strand at 145.151 kb on - strand at 145.151 kb on - strand at 145.216 kb on - strand at 145.216 kb on - strand at 145.216 kb on - strand at 145.241 kb on + strand at 145.241 kb on + strand at 145.278 kb on - strand at 145.516 kb on + strand, within ABIE40_RS20220 at 145.516 kb on + strand, within ABIE40_RS20220 at 146.024 kb on + strand, within ABIE40_RS20220 at 146.025 kb on - strand, within ABIE40_RS20220 at 146.075 kb on + strand, within ABIE40_RS20220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 143,598 - ABIE40_RS20210 0.32 -1.5 143,598 - ABIE40_RS20210 0.32 -0.5 143,630 + ABIE40_RS20210 0.35 -0.4 143,790 - ABIE40_RS20210 0.48 +2.5 143,790 - ABIE40_RS20210 0.48 +0.4 144,327 - -0.4 144,475 + -3.6 144,771 + ABIE40_RS20215 0.35 +0.3 144,985 + ABIE40_RS20215 0.69 +1.3 144,985 + ABIE40_RS20215 0.69 +0.2 144,985 + ABIE40_RS20215 0.69 +1.0 145,150 + +0.7 145,151 - -0.5 145,151 - -0.9 145,216 - +1.0 145,216 - +0.1 145,216 - +0.0 145,241 + -0.5 145,241 + -0.1 145,278 - +1.6 145,516 + ABIE40_RS20220 0.25 +0.3 145,516 + ABIE40_RS20220 0.25 +0.2 146,024 + ABIE40_RS20220 0.66 +0.5 146,025 - ABIE40_RS20220 0.66 -0.8 146,075 + ABIE40_RS20220 0.71 +1.1
Or see this region's nucleotide sequence