Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS29615 and ABIE40_RS29620 overlap by 4 nucleotides ABIE40_RS29620 and ABIE40_RS29625 are separated by 24 nucleotides ABIE40_RS29625 and ABIE40_RS29630 overlap by 1 nucleotides ABIE40_RS29630 and ABIE40_RS29635 overlap by 8 nucleotides
ABIE40_RS29615: ABIE40_RS29615 - dihydropyrimidinase, at 618,981 to 620,414
_RS29615
ABIE40_RS29620: ABIE40_RS29620 - SDR family oxidoreductase, at 620,411 to 621,169
_RS29620
ABIE40_RS29625: ABIE40_RS29625 - NAD(P)-dependent oxidoreductase, at 621,194 to 622,231
_RS29625
ABIE40_RS29630: ABIE40_RS29630 - allantoinase PuuE, at 622,231 to 623,139
_RS29630
ABIE40_RS29635: ABIE40_RS29635 - thiamine pyrophosphate-requiring protein, at 623,132 to 624,823
_RS29635
Position (kb)
621
622
623 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 620.242 kb on - strand, within ABIE40_RS29615 at 620.250 kb on + strand, within ABIE40_RS29615 at 620.251 kb on - strand, within ABIE40_RS29615 at 620.251 kb on - strand, within ABIE40_RS29615 at 620.251 kb on - strand, within ABIE40_RS29615 at 620.442 kb on + strand at 620.443 kb on - strand at 620.515 kb on + strand, within ABIE40_RS29620 at 620.515 kb on + strand, within ABIE40_RS29620 at 620.516 kb on - strand, within ABIE40_RS29620 at 620.516 kb on - strand, within ABIE40_RS29620 at 620.516 kb on - strand, within ABIE40_RS29620 at 620.518 kb on + strand, within ABIE40_RS29620 at 620.518 kb on + strand, within ABIE40_RS29620 at 620.519 kb on - strand, within ABIE40_RS29620 at 620.609 kb on - strand, within ABIE40_RS29620 at 620.678 kb on - strand, within ABIE40_RS29620 at 620.798 kb on - strand, within ABIE40_RS29620 at 620.877 kb on - strand, within ABIE40_RS29620 at 620.944 kb on + strand, within ABIE40_RS29620 at 620.944 kb on + strand, within ABIE40_RS29620 at 621.156 kb on - strand at 621.349 kb on + strand, within ABIE40_RS29625 at 621.357 kb on + strand, within ABIE40_RS29625 at 621.357 kb on + strand, within ABIE40_RS29625 at 621.368 kb on - strand, within ABIE40_RS29625 at 621.368 kb on - strand, within ABIE40_RS29625 at 621.659 kb on + strand, within ABIE40_RS29625 at 621.660 kb on - strand, within ABIE40_RS29625 at 621.660 kb on - strand, within ABIE40_RS29625 at 621.695 kb on + strand, within ABIE40_RS29625 at 621.695 kb on + strand, within ABIE40_RS29625 at 621.695 kb on + strand, within ABIE40_RS29625 at 621.894 kb on - strand, within ABIE40_RS29625 at 621.894 kb on - strand, within ABIE40_RS29625 at 621.894 kb on - strand, within ABIE40_RS29625 at 621.966 kb on - strand, within ABIE40_RS29625 at 621.966 kb on - strand, within ABIE40_RS29625 at 621.966 kb on - strand, within ABIE40_RS29625 at 621.966 kb on - strand, within ABIE40_RS29625 at 621.966 kb on - strand, within ABIE40_RS29625 at 621.970 kb on + strand, within ABIE40_RS29625 at 622.027 kb on + strand, within ABIE40_RS29625 at 622.027 kb on + strand, within ABIE40_RS29625 at 622.028 kb on - strand, within ABIE40_RS29625 at 622.038 kb on + strand, within ABIE40_RS29625 at 622.115 kb on + strand, within ABIE40_RS29625 at 622.116 kb on - strand, within ABIE40_RS29625 at 622.116 kb on - strand, within ABIE40_RS29625 at 622.116 kb on - strand, within ABIE40_RS29625 at 622.184 kb on + strand at 622.184 kb on + strand at 622.185 kb on - strand at 622.185 kb on - strand at 622.185 kb on - strand at 622.342 kb on + strand, within ABIE40_RS29630 at 622.402 kb on + strand, within ABIE40_RS29630 at 622.402 kb on + strand, within ABIE40_RS29630 at 622.402 kb on + strand, within ABIE40_RS29630 at 622.403 kb on - strand, within ABIE40_RS29630 at 622.403 kb on - strand, within ABIE40_RS29630 at 622.403 kb on - strand, within ABIE40_RS29630 at 622.405 kb on - strand, within ABIE40_RS29630 at 622.407 kb on + strand, within ABIE40_RS29630 at 622.507 kb on + strand, within ABIE40_RS29630 at 622.783 kb on + strand, within ABIE40_RS29630 at 622.786 kb on + strand, within ABIE40_RS29630 at 622.885 kb on + strand, within ABIE40_RS29630 at 622.903 kb on + strand, within ABIE40_RS29630 at 622.951 kb on + strand, within ABIE40_RS29630 at 622.951 kb on + strand, within ABIE40_RS29630 at 622.953 kb on + strand, within ABIE40_RS29630 at 622.953 kb on + strand, within ABIE40_RS29630 at 622.954 kb on - strand, within ABIE40_RS29630 at 622.954 kb on - strand, within ABIE40_RS29630 at 622.954 kb on - strand, within ABIE40_RS29630 at 623.004 kb on - strand, within ABIE40_RS29630 at 623.004 kb on - strand, within ABIE40_RS29630 at 623.107 kb on - strand at 623.140 kb on + strand at 623.141 kb on - strand at 623.141 kb on - strand at 623.141 kb on - strand at 623.141 kb on - strand at 623.147 kb on + strand at 623.147 kb on + strand at 623.148 kb on - strand at 623.148 kb on - strand at 623.148 kb on - strand at 623.205 kb on - strand at 623.205 kb on - strand at 623.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 620,242 - ABIE40_RS29615 0.88 +0.5 620,250 + ABIE40_RS29615 0.88 +1.2 620,251 - ABIE40_RS29615 0.89 +1.2 620,251 - ABIE40_RS29615 0.89 -1.3 620,251 - ABIE40_RS29615 0.89 +1.9 620,442 + -1.8 620,443 - +0.3 620,515 + ABIE40_RS29620 0.14 +1.0 620,515 + ABIE40_RS29620 0.14 -1.8 620,516 - ABIE40_RS29620 0.14 -0.9 620,516 - ABIE40_RS29620 0.14 +0.3 620,516 - ABIE40_RS29620 0.14 -0.1 620,518 + ABIE40_RS29620 0.14 +0.5 620,518 + ABIE40_RS29620 0.14 +0.2 620,519 - ABIE40_RS29620 0.14 -1.7 620,609 - ABIE40_RS29620 0.26 +0.4 620,678 - ABIE40_RS29620 0.35 +0.8 620,798 - ABIE40_RS29620 0.51 +0.3 620,877 - ABIE40_RS29620 0.61 -1.6 620,944 + ABIE40_RS29620 0.70 -0.3 620,944 + ABIE40_RS29620 0.70 +1.7 621,156 - +1.1 621,349 + ABIE40_RS29625 0.15 -1.9 621,357 + ABIE40_RS29625 0.16 +0.2 621,357 + ABIE40_RS29625 0.16 -0.4 621,368 - ABIE40_RS29625 0.17 +0.4 621,368 - ABIE40_RS29625 0.17 +3.2 621,659 + ABIE40_RS29625 0.45 -0.1 621,660 - ABIE40_RS29625 0.45 +0.2 621,660 - ABIE40_RS29625 0.45 -2.5 621,695 + ABIE40_RS29625 0.48 +1.2 621,695 + ABIE40_RS29625 0.48 -2.1 621,695 + ABIE40_RS29625 0.48 +0.2 621,894 - ABIE40_RS29625 0.67 -0.3 621,894 - ABIE40_RS29625 0.67 +0.2 621,894 - ABIE40_RS29625 0.67 +1.2 621,966 - ABIE40_RS29625 0.74 -1.6 621,966 - ABIE40_RS29625 0.74 +0.9 621,966 - ABIE40_RS29625 0.74 +0.2 621,966 - ABIE40_RS29625 0.74 +0.1 621,966 - ABIE40_RS29625 0.74 -1.6 621,970 + ABIE40_RS29625 0.75 +1.2 622,027 + ABIE40_RS29625 0.80 -0.2 622,027 + ABIE40_RS29625 0.80 -0.5 622,028 - ABIE40_RS29625 0.80 -0.9 622,038 + ABIE40_RS29625 0.81 -0.8 622,115 + ABIE40_RS29625 0.89 +0.6 622,116 - ABIE40_RS29625 0.89 +0.3 622,116 - ABIE40_RS29625 0.89 +0.5 622,116 - ABIE40_RS29625 0.89 -1.9 622,184 + -0.9 622,184 + +0.4 622,185 - -1.8 622,185 - -1.6 622,185 - +3.5 622,342 + ABIE40_RS29630 0.12 +0.3 622,402 + ABIE40_RS29630 0.19 +2.2 622,402 + ABIE40_RS29630 0.19 +2.2 622,402 + ABIE40_RS29630 0.19 -3.1 622,403 - ABIE40_RS29630 0.19 -0.8 622,403 - ABIE40_RS29630 0.19 +0.6 622,403 - ABIE40_RS29630 0.19 +2.2 622,405 - ABIE40_RS29630 0.19 +1.2 622,407 + ABIE40_RS29630 0.19 +0.9 622,507 + ABIE40_RS29630 0.30 -0.6 622,783 + ABIE40_RS29630 0.61 -0.4 622,786 + ABIE40_RS29630 0.61 -0.4 622,885 + ABIE40_RS29630 0.72 -1.1 622,903 + ABIE40_RS29630 0.74 +2.2 622,951 + ABIE40_RS29630 0.79 +1.2 622,951 + ABIE40_RS29630 0.79 -2.2 622,953 + ABIE40_RS29630 0.79 -0.6 622,953 + ABIE40_RS29630 0.79 +2.2 622,954 - ABIE40_RS29630 0.80 +0.4 622,954 - ABIE40_RS29630 0.80 -0.1 622,954 - ABIE40_RS29630 0.80 -0.7 623,004 - ABIE40_RS29630 0.85 +2.2 623,004 - ABIE40_RS29630 0.85 +0.3 623,107 - -0.8 623,140 + -0.1 623,141 - -0.4 623,141 - -1.1 623,141 - -0.8 623,141 - -0.4 623,147 + +0.5 623,147 + +0.7 623,148 - +0.2 623,148 - -1.9 623,148 - +1.1 623,205 - -0.4 623,205 - +2.2 623,206 + -0.8
Or see this region's nucleotide sequence