Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS16670 and ABIE40_RS16675 are separated by 136 nucleotides ABIE40_RS16675 and ABIE40_RS16680 are separated by 45 nucleotides ABIE40_RS16680 and ABIE40_RS16685 are separated by 88 nucleotides
ABIE40_RS16670: ABIE40_RS16670 - serine hydrolase, at 3,321,332 to 3,322,336
_RS16670
ABIE40_RS16675: ABIE40_RS16675 - NAD-dependent epimerase/dehydratase family protein, at 3,322,473 to 3,323,429
_RS16675
ABIE40_RS16680: ABIE40_RS16680 - N-acetyltransferase, at 3,323,475 to 3,324,065
_RS16680
ABIE40_RS16685: ABIE40_RS16685 - ornithine/lysine decarboxylase, at 3,324,154 to 3,325,287
_RS16685
Position (kb)
3322
3323
3324 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3321.481 kb on - strand, within ABIE40_RS16670 at 3321.481 kb on - strand, within ABIE40_RS16670 at 3321.534 kb on + strand, within ABIE40_RS16670 at 3321.534 kb on + strand, within ABIE40_RS16670 at 3321.534 kb on + strand, within ABIE40_RS16670 at 3321.534 kb on + strand, within ABIE40_RS16670 at 3321.534 kb on + strand, within ABIE40_RS16670 at 3321.534 kb on + strand, within ABIE40_RS16670 at 3321.535 kb on - strand, within ABIE40_RS16670 at 3321.535 kb on - strand, within ABIE40_RS16670 at 3321.535 kb on - strand, within ABIE40_RS16670 at 3321.535 kb on - strand, within ABIE40_RS16670 at 3321.540 kb on + strand, within ABIE40_RS16670 at 3321.541 kb on - strand, within ABIE40_RS16670 at 3321.590 kb on - strand, within ABIE40_RS16670 at 3321.646 kb on - strand, within ABIE40_RS16670 at 3321.646 kb on - strand, within ABIE40_RS16670 at 3321.705 kb on + strand, within ABIE40_RS16670 at 3321.706 kb on - strand, within ABIE40_RS16670 at 3321.706 kb on - strand, within ABIE40_RS16670 at 3321.706 kb on - strand, within ABIE40_RS16670 at 3321.706 kb on - strand, within ABIE40_RS16670 at 3321.793 kb on + strand, within ABIE40_RS16670 at 3321.794 kb on - strand, within ABIE40_RS16670 at 3321.794 kb on - strand, within ABIE40_RS16670 at 3321.794 kb on - strand, within ABIE40_RS16670 at 3322.067 kb on + strand, within ABIE40_RS16670 at 3322.067 kb on + strand, within ABIE40_RS16670 at 3322.068 kb on - strand, within ABIE40_RS16670 at 3322.068 kb on - strand, within ABIE40_RS16670 at 3322.068 kb on - strand, within ABIE40_RS16670 at 3322.068 kb on - strand, within ABIE40_RS16670 at 3322.084 kb on + strand, within ABIE40_RS16670 at 3322.084 kb on + strand, within ABIE40_RS16670 at 3322.084 kb on + strand, within ABIE40_RS16670 at 3322.085 kb on - strand, within ABIE40_RS16670 at 3322.153 kb on + strand, within ABIE40_RS16670 at 3322.153 kb on + strand, within ABIE40_RS16670 at 3322.153 kb on + strand, within ABIE40_RS16670 at 3322.153 kb on + strand, within ABIE40_RS16670 at 3322.153 kb on + strand, within ABIE40_RS16670 at 3322.154 kb on - strand, within ABIE40_RS16670 at 3322.154 kb on - strand, within ABIE40_RS16670 at 3322.154 kb on - strand, within ABIE40_RS16670 at 3322.164 kb on + strand, within ABIE40_RS16670 at 3322.165 kb on - strand, within ABIE40_RS16670 at 3322.165 kb on - strand, within ABIE40_RS16670 at 3322.347 kb on + strand at 3322.347 kb on + strand at 3322.347 kb on + strand at 3322.348 kb on - strand at 3322.348 kb on - strand at 3322.348 kb on - strand at 3322.388 kb on - strand at 3322.388 kb on - strand at 3322.388 kb on - strand at 3322.408 kb on + strand at 3322.408 kb on + strand at 3322.408 kb on + strand at 3322.408 kb on + strand at 3322.409 kb on - strand at 3322.445 kb on + strand at 3322.445 kb on + strand at 3322.445 kb on + strand at 3322.445 kb on + strand at 3322.446 kb on - strand at 3322.446 kb on - strand at 3322.446 kb on - strand at 3322.446 kb on - strand at 3322.468 kb on + strand at 3322.468 kb on + strand at 3322.468 kb on + strand at 3322.468 kb on + strand at 3322.468 kb on + strand at 3322.468 kb on + strand at 3322.468 kb on + strand at 3322.469 kb on - strand at 3322.472 kb on + strand at 3322.472 kb on + strand at 3322.472 kb on + strand at 3322.472 kb on + strand at 3322.472 kb on + strand at 3322.473 kb on - strand at 3322.473 kb on - strand at 3322.473 kb on - strand at 3322.473 kb on - strand at 3322.518 kb on + strand at 3322.518 kb on + strand at 3322.518 kb on + strand at 3322.519 kb on - strand at 3322.587 kb on + strand, within ABIE40_RS16675 at 3322.588 kb on - strand, within ABIE40_RS16675 at 3322.658 kb on + strand, within ABIE40_RS16675 at 3322.658 kb on + strand, within ABIE40_RS16675 at 3322.659 kb on - strand, within ABIE40_RS16675 at 3322.841 kb on - strand, within ABIE40_RS16675 at 3322.841 kb on - strand, within ABIE40_RS16675 at 3322.860 kb on + strand, within ABIE40_RS16675 at 3322.867 kb on + strand, within ABIE40_RS16675 at 3322.867 kb on + strand, within ABIE40_RS16675 at 3322.867 kb on + strand, within ABIE40_RS16675 at 3322.867 kb on + strand, within ABIE40_RS16675 at 3322.867 kb on + strand, within ABIE40_RS16675 at 3322.868 kb on - strand, within ABIE40_RS16675 at 3322.868 kb on - strand, within ABIE40_RS16675 at 3322.868 kb on - strand, within ABIE40_RS16675 at 3322.868 kb on - strand, within ABIE40_RS16675 at 3322.890 kb on + strand, within ABIE40_RS16675 at 3322.890 kb on + strand, within ABIE40_RS16675 at 3322.891 kb on - strand, within ABIE40_RS16675 at 3322.891 kb on - strand, within ABIE40_RS16675 at 3322.891 kb on - strand, within ABIE40_RS16675 at 3323.199 kb on + strand, within ABIE40_RS16675 at 3323.199 kb on + strand, within ABIE40_RS16675 at 3323.199 kb on + strand, within ABIE40_RS16675 at 3323.199 kb on + strand, within ABIE40_RS16675 at 3323.199 kb on + strand, within ABIE40_RS16675 at 3323.199 kb on + strand, within ABIE40_RS16675 at 3323.199 kb on + strand, within ABIE40_RS16675 at 3323.200 kb on - strand, within ABIE40_RS16675 at 3323.200 kb on - strand, within ABIE40_RS16675 at 3323.200 kb on - strand, within ABIE40_RS16675 at 3323.200 kb on - strand, within ABIE40_RS16675 at 3323.200 kb on - strand, within ABIE40_RS16675 at 3323.200 kb on - strand, within ABIE40_RS16675 at 3323.200 kb on - strand, within ABIE40_RS16675 at 3323.200 kb on - strand, within ABIE40_RS16675 at 3323.241 kb on + strand, within ABIE40_RS16675 at 3323.242 kb on - strand, within ABIE40_RS16675 at 3323.242 kb on - strand, within ABIE40_RS16675 at 3323.361 kb on + strand at 3323.362 kb on - strand at 3323.651 kb on - strand, within ABIE40_RS16680 at 3323.651 kb on - strand, within ABIE40_RS16680 at 3323.651 kb on - strand, within ABIE40_RS16680 at 3323.651 kb on - strand, within ABIE40_RS16680 at 3323.651 kb on - strand, within ABIE40_RS16680 at 3323.651 kb on - strand, within ABIE40_RS16680 at 3323.651 kb on - strand, within ABIE40_RS16680 at 3323.653 kb on + strand, within ABIE40_RS16680 at 3323.654 kb on - strand, within ABIE40_RS16680 at 3323.654 kb on - strand, within ABIE40_RS16680 at 3323.829 kb on + strand, within ABIE40_RS16680 at 3323.903 kb on + strand, within ABIE40_RS16680 at 3323.904 kb on - strand, within ABIE40_RS16680 at 3323.904 kb on - strand, within ABIE40_RS16680 at 3324.090 kb on + strand at 3324.090 kb on + strand at 3324.090 kb on + strand at 3324.090 kb on + strand at 3324.091 kb on - strand at 3324.091 kb on - strand at 3324.091 kb on - strand at 3324.095 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 3,321,481 - ABIE40_RS16670 0.15 +0.5 3,321,481 - ABIE40_RS16670 0.15 +0.3 3,321,534 + ABIE40_RS16670 0.20 -1.1 3,321,534 + ABIE40_RS16670 0.20 -3.5 3,321,534 + ABIE40_RS16670 0.20 +0.7 3,321,534 + ABIE40_RS16670 0.20 -1.3 3,321,534 + ABIE40_RS16670 0.20 -1.2 3,321,534 + ABIE40_RS16670 0.20 -1.6 3,321,535 - ABIE40_RS16670 0.20 +0.0 3,321,535 - ABIE40_RS16670 0.20 -0.4 3,321,535 - ABIE40_RS16670 0.20 -0.2 3,321,535 - ABIE40_RS16670 0.20 -0.3 3,321,540 + ABIE40_RS16670 0.21 -1.8 3,321,541 - ABIE40_RS16670 0.21 -0.2 3,321,590 - ABIE40_RS16670 0.26 +0.0 3,321,646 - ABIE40_RS16670 0.31 +0.5 3,321,646 - ABIE40_RS16670 0.31 -0.2 3,321,705 + ABIE40_RS16670 0.37 -0.9 3,321,706 - ABIE40_RS16670 0.37 +0.5 3,321,706 - ABIE40_RS16670 0.37 +0.0 3,321,706 - ABIE40_RS16670 0.37 +0.7 3,321,706 - ABIE40_RS16670 0.37 -2.2 3,321,793 + ABIE40_RS16670 0.46 -1.8 3,321,794 - ABIE40_RS16670 0.46 +1.7 3,321,794 - ABIE40_RS16670 0.46 +0.2 3,321,794 - ABIE40_RS16670 0.46 -1.0 3,322,067 + ABIE40_RS16670 0.73 -1.3 3,322,067 + ABIE40_RS16670 0.73 +0.9 3,322,068 - ABIE40_RS16670 0.73 -1.5 3,322,068 - ABIE40_RS16670 0.73 +0.4 3,322,068 - ABIE40_RS16670 0.73 -0.3 3,322,068 - ABIE40_RS16670 0.73 +0.6 3,322,084 + ABIE40_RS16670 0.75 +0.4 3,322,084 + ABIE40_RS16670 0.75 -1.0 3,322,084 + ABIE40_RS16670 0.75 +0.5 3,322,085 - ABIE40_RS16670 0.75 -0.5 3,322,153 + ABIE40_RS16670 0.82 -0.9 3,322,153 + ABIE40_RS16670 0.82 -0.4 3,322,153 + ABIE40_RS16670 0.82 -0.9 3,322,153 + ABIE40_RS16670 0.82 -2.0 3,322,153 + ABIE40_RS16670 0.82 -1.8 3,322,154 - ABIE40_RS16670 0.82 +0.2 3,322,154 - ABIE40_RS16670 0.82 +0.1 3,322,154 - ABIE40_RS16670 0.82 -2.5 3,322,164 + ABIE40_RS16670 0.83 +0.2 3,322,165 - ABIE40_RS16670 0.83 +0.3 3,322,165 - ABIE40_RS16670 0.83 -0.2 3,322,347 + +0.3 3,322,347 + -0.4 3,322,347 + -1.8 3,322,348 - -0.5 3,322,348 - +0.9 3,322,348 - -0.3 3,322,388 - -0.7 3,322,388 - +0.1 3,322,388 - +0.5 3,322,408 + +0.2 3,322,408 + +1.7 3,322,408 + -1.0 3,322,408 + +0.3 3,322,409 - -1.7 3,322,445 + -0.1 3,322,445 + -0.9 3,322,445 + +0.2 3,322,445 + -1.1 3,322,446 - +1.0 3,322,446 - -1.3 3,322,446 - -2.5 3,322,446 - +0.6 3,322,468 + +1.7 3,322,468 + -0.7 3,322,468 + +2.5 3,322,468 + -1.4 3,322,468 + +0.2 3,322,468 + +0.2 3,322,468 + -2.0 3,322,469 - +0.1 3,322,472 + -3.0 3,322,472 + -1.7 3,322,472 + -0.3 3,322,472 + +0.3 3,322,472 + +0.5 3,322,473 - +1.0 3,322,473 - +0.2 3,322,473 - -1.5 3,322,473 - -0.3 3,322,518 + -0.0 3,322,518 + -0.2 3,322,518 + +0.1 3,322,519 - -0.5 3,322,587 + ABIE40_RS16675 0.12 -0.7 3,322,588 - ABIE40_RS16675 0.12 +0.0 3,322,658 + ABIE40_RS16675 0.19 +0.2 3,322,658 + ABIE40_RS16675 0.19 -2.0 3,322,659 - ABIE40_RS16675 0.19 -1.2 3,322,841 - ABIE40_RS16675 0.38 -2.4 3,322,841 - ABIE40_RS16675 0.38 +0.6 3,322,860 + ABIE40_RS16675 0.40 -0.8 3,322,867 + ABIE40_RS16675 0.41 +2.5 3,322,867 + ABIE40_RS16675 0.41 +0.7 3,322,867 + ABIE40_RS16675 0.41 -0.3 3,322,867 + ABIE40_RS16675 0.41 +0.0 3,322,867 + ABIE40_RS16675 0.41 -0.8 3,322,868 - ABIE40_RS16675 0.41 -1.1 3,322,868 - ABIE40_RS16675 0.41 -1.4 3,322,868 - ABIE40_RS16675 0.41 -0.4 3,322,868 - ABIE40_RS16675 0.41 -1.3 3,322,890 + ABIE40_RS16675 0.44 +0.3 3,322,890 + ABIE40_RS16675 0.44 +0.6 3,322,891 - ABIE40_RS16675 0.44 -0.8 3,322,891 - ABIE40_RS16675 0.44 -0.2 3,322,891 - ABIE40_RS16675 0.44 -0.3 3,323,199 + ABIE40_RS16675 0.76 +0.2 3,323,199 + ABIE40_RS16675 0.76 +0.1 3,323,199 + ABIE40_RS16675 0.76 -1.3 3,323,199 + ABIE40_RS16675 0.76 -1.1 3,323,199 + ABIE40_RS16675 0.76 +0.1 3,323,199 + ABIE40_RS16675 0.76 +1.6 3,323,199 + ABIE40_RS16675 0.76 -1.0 3,323,200 - ABIE40_RS16675 0.76 +1.6 3,323,200 - ABIE40_RS16675 0.76 -0.4 3,323,200 - ABIE40_RS16675 0.76 -2.2 3,323,200 - ABIE40_RS16675 0.76 +0.6 3,323,200 - ABIE40_RS16675 0.76 -0.4 3,323,200 - ABIE40_RS16675 0.76 +0.7 3,323,200 - ABIE40_RS16675 0.76 -2.7 3,323,200 - ABIE40_RS16675 0.76 +2.1 3,323,241 + ABIE40_RS16675 0.80 +1.1 3,323,242 - ABIE40_RS16675 0.80 -0.6 3,323,242 - ABIE40_RS16675 0.80 +0.4 3,323,361 + -0.0 3,323,362 - +0.7 3,323,651 - ABIE40_RS16680 0.30 +0.6 3,323,651 - ABIE40_RS16680 0.30 +0.1 3,323,651 - ABIE40_RS16680 0.30 -0.2 3,323,651 - ABIE40_RS16680 0.30 -0.2 3,323,651 - ABIE40_RS16680 0.30 +0.4 3,323,651 - ABIE40_RS16680 0.30 -0.4 3,323,651 - ABIE40_RS16680 0.30 -1.6 3,323,653 + ABIE40_RS16680 0.30 +0.7 3,323,654 - ABIE40_RS16680 0.30 +0.4 3,323,654 - ABIE40_RS16680 0.30 -0.6 3,323,829 + ABIE40_RS16680 0.60 +3.3 3,323,903 + ABIE40_RS16680 0.72 +0.2 3,323,904 - ABIE40_RS16680 0.73 +0.4 3,323,904 - ABIE40_RS16680 0.73 -0.3 3,324,090 + -0.3 3,324,090 + -0.3 3,324,090 + -1.4 3,324,090 + +0.8 3,324,091 - -2.4 3,324,091 - +0.0 3,324,091 - -0.8 3,324,095 - -2.2
Or see this region's nucleotide sequence