Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS08660 and ABIE40_RS08665 are separated by 10 nucleotides ABIE40_RS08665 and ABIE40_RS08670 are separated by 2 nucleotides ABIE40_RS08670 and ABIE40_RS08675 are separated by 20 nucleotides ABIE40_RS08675 and ABIE40_RS08680 are separated by 9 nucleotides
ABIE40_RS08660: ABIE40_RS08660 - glucose-1-phosphate thymidylyltransferase RfbA, at 1,673,346 to 1,674,215
_RS08660
ABIE40_RS08665: ABIE40_RS08665 - dTDP-4-dehydrorhamnose reductase, at 1,674,226 to 1,675,113
_RS08665
ABIE40_RS08670: ABIE40_RS08670 - dTDP-glucose 4,6-dehydratase, at 1,675,116 to 1,676,171
_RS08670
ABIE40_RS08675: ABIE40_RS08675 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 1,676,192 to 1,676,758
_RS08675
ABIE40_RS08680: ABIE40_RS08680 - glycosyltransferase, at 1,676,768 to 1,678,744
_RS08680
Position (kb) 1675
1676
1677 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1674.299 kb on + strand at 1674.299 kb on + strand at 1674.299 kb on + strand at 1674.299 kb on + strand at 1674.299 kb on + strand at 1674.299 kb on + strand at 1674.299 kb on + strand at 1674.300 kb on - strand at 1674.300 kb on - strand at 1674.300 kb on - strand at 1674.362 kb on + strand, within ABIE40_RS08665 at 1674.362 kb on + strand, within ABIE40_RS08665 at 1674.523 kb on - strand, within ABIE40_RS08665 at 1674.659 kb on + strand, within ABIE40_RS08665 at 1674.660 kb on - strand, within ABIE40_RS08665 at 1674.660 kb on - strand, within ABIE40_RS08665 at 1674.660 kb on - strand, within ABIE40_RS08665 at 1674.660 kb on - strand, within ABIE40_RS08665 at 1674.733 kb on - strand, within ABIE40_RS08665 at 1674.733 kb on - strand, within ABIE40_RS08665 at 1674.733 kb on - strand, within ABIE40_RS08665 at 1674.740 kb on + strand, within ABIE40_RS08665 at 1674.746 kb on + strand, within ABIE40_RS08665 at 1674.776 kb on + strand, within ABIE40_RS08665 at 1674.777 kb on - strand, within ABIE40_RS08665 at 1674.777 kb on - strand, within ABIE40_RS08665 at 1674.789 kb on + strand, within ABIE40_RS08665 at 1674.802 kb on + strand, within ABIE40_RS08665 at 1674.802 kb on + strand, within ABIE40_RS08665 at 1674.802 kb on + strand, within ABIE40_RS08665 at 1674.802 kb on + strand, within ABIE40_RS08665 at 1674.802 kb on + strand, within ABIE40_RS08665 at 1674.802 kb on + strand, within ABIE40_RS08665 at 1674.803 kb on - strand, within ABIE40_RS08665 at 1674.803 kb on - strand, within ABIE40_RS08665 at 1674.803 kb on - strand, within ABIE40_RS08665 at 1674.803 kb on - strand, within ABIE40_RS08665 at 1674.806 kb on + strand, within ABIE40_RS08665 at 1674.806 kb on + strand, within ABIE40_RS08665 at 1674.806 kb on + strand, within ABIE40_RS08665 at 1674.807 kb on - strand, within ABIE40_RS08665 at 1674.807 kb on - strand, within ABIE40_RS08665 at 1674.931 kb on - strand, within ABIE40_RS08665 at 1674.933 kb on - strand, within ABIE40_RS08665 at 1674.973 kb on + strand, within ABIE40_RS08665 at 1675.002 kb on + strand, within ABIE40_RS08665 at 1675.002 kb on + strand, within ABIE40_RS08665 at 1675.153 kb on + strand at 1675.153 kb on + strand at 1675.153 kb on + strand at 1675.154 kb on - strand at 1675.154 kb on - strand at 1675.154 kb on - strand at 1675.154 kb on - strand at 1675.155 kb on + strand at 1675.198 kb on + strand at 1675.198 kb on + strand at 1675.198 kb on + strand at 1675.199 kb on - strand at 1675.288 kb on + strand, within ABIE40_RS08670 at 1675.288 kb on + strand, within ABIE40_RS08670 at 1675.359 kb on + strand, within ABIE40_RS08670 at 1675.360 kb on - strand, within ABIE40_RS08670 at 1675.360 kb on - strand, within ABIE40_RS08670 at 1675.360 kb on - strand, within ABIE40_RS08670 at 1675.432 kb on + strand, within ABIE40_RS08670 at 1675.432 kb on + strand, within ABIE40_RS08670 at 1675.433 kb on - strand, within ABIE40_RS08670 at 1675.433 kb on - strand, within ABIE40_RS08670 at 1675.495 kb on + strand, within ABIE40_RS08670 at 1675.495 kb on + strand, within ABIE40_RS08670 at 1675.495 kb on + strand, within ABIE40_RS08670 at 1675.496 kb on - strand, within ABIE40_RS08670 at 1675.496 kb on - strand, within ABIE40_RS08670 at 1675.496 kb on - strand, within ABIE40_RS08670 at 1675.496 kb on - strand, within ABIE40_RS08670 at 1675.496 kb on - strand, within ABIE40_RS08670 at 1675.606 kb on + strand, within ABIE40_RS08670 at 1675.607 kb on - strand, within ABIE40_RS08670 at 1675.607 kb on - strand, within ABIE40_RS08670 at 1675.645 kb on + strand, within ABIE40_RS08670 at 1675.645 kb on + strand, within ABIE40_RS08670 at 1675.645 kb on + strand, within ABIE40_RS08670 at 1675.646 kb on - strand, within ABIE40_RS08670 at 1675.646 kb on - strand, within ABIE40_RS08670 at 1675.717 kb on + strand, within ABIE40_RS08670 at 1675.762 kb on + strand, within ABIE40_RS08670 at 1675.762 kb on + strand, within ABIE40_RS08670 at 1675.762 kb on + strand, within ABIE40_RS08670 at 1675.762 kb on + strand, within ABIE40_RS08670 at 1675.763 kb on - strand, within ABIE40_RS08670 at 1675.763 kb on - strand, within ABIE40_RS08670 at 1675.763 kb on - strand, within ABIE40_RS08670 at 1675.871 kb on + strand, within ABIE40_RS08670 at 1675.871 kb on + strand, within ABIE40_RS08670 at 1675.872 kb on - strand, within ABIE40_RS08670 at 1675.872 kb on - strand, within ABIE40_RS08670 at 1675.998 kb on - strand, within ABIE40_RS08670 at 1676.065 kb on + strand, within ABIE40_RS08670 at 1676.065 kb on + strand, within ABIE40_RS08670 at 1676.066 kb on - strand at 1676.066 kb on - strand at 1676.214 kb on + strand at 1676.214 kb on + strand at 1676.215 kb on - strand at 1676.215 kb on - strand at 1676.215 kb on - strand at 1676.303 kb on - strand, within ABIE40_RS08675 at 1676.345 kb on - strand, within ABIE40_RS08675 at 1676.346 kb on + strand, within ABIE40_RS08675 at 1676.346 kb on + strand, within ABIE40_RS08675 at 1676.346 kb on + strand, within ABIE40_RS08675 at 1676.346 kb on + strand, within ABIE40_RS08675 at 1676.346 kb on + strand, within ABIE40_RS08675 at 1676.364 kb on + strand, within ABIE40_RS08675 at 1676.365 kb on - strand, within ABIE40_RS08675 at 1676.365 kb on - strand, within ABIE40_RS08675 at 1676.377 kb on + strand, within ABIE40_RS08675 at 1676.377 kb on + strand, within ABIE40_RS08675 at 1676.685 kb on + strand, within ABIE40_RS08675 at 1676.685 kb on + strand, within ABIE40_RS08675 at 1676.769 kb on + strand at 1676.770 kb on - strand at 1676.770 kb on - strand at 1676.770 kb on - strand at 1676.830 kb on + strand at 1676.830 kb on + strand at 1676.830 kb on + strand at 1676.830 kb on + strand at 1676.830 kb on + strand at 1676.831 kb on - strand at 1676.831 kb on - strand at 1676.831 kb on - strand at 1676.831 kb on - strand at 1676.831 kb on - strand at 1676.832 kb on + strand at 1676.833 kb on - strand at 1676.908 kb on - strand at 1676.908 kb on - strand at 1677.000 kb on + strand, within ABIE40_RS08680 at 1677.001 kb on - strand, within ABIE40_RS08680 at 1677.036 kb on + strand, within ABIE40_RS08680 at 1677.036 kb on + strand, within ABIE40_RS08680 at 1677.036 kb on + strand, within ABIE40_RS08680 at 1677.037 kb on - strand, within ABIE40_RS08680 at 1677.046 kb on + strand, within ABIE40_RS08680 at 1677.046 kb on + strand, within ABIE40_RS08680 at 1677.046 kb on + strand, within ABIE40_RS08680 at 1677.046 kb on + strand, within ABIE40_RS08680 at 1677.096 kb on + strand, within ABIE40_RS08680 at 1677.096 kb on + strand, within ABIE40_RS08680 at 1677.096 kb on + strand, within ABIE40_RS08680 at 1677.096 kb on + strand, within ABIE40_RS08680 at 1677.097 kb on - strand, within ABIE40_RS08680 at 1677.166 kb on - strand, within ABIE40_RS08680
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 1,674,299 + -0.4 1,674,299 + +0.4 1,674,299 + +0.3 1,674,299 + +0.4 1,674,299 + -0.4 1,674,299 + +0.0 1,674,299 + -0.4 1,674,300 - +0.8 1,674,300 - -0.9 1,674,300 - -0.1 1,674,362 + ABIE40_RS08665 0.15 -2.3 1,674,362 + ABIE40_RS08665 0.15 +0.9 1,674,523 - ABIE40_RS08665 0.33 -3.3 1,674,659 + ABIE40_RS08665 0.49 +0.4 1,674,660 - ABIE40_RS08665 0.49 +0.7 1,674,660 - ABIE40_RS08665 0.49 +0.0 1,674,660 - ABIE40_RS08665 0.49 +0.5 1,674,660 - ABIE40_RS08665 0.49 -1.4 1,674,733 - ABIE40_RS08665 0.57 -1.2 1,674,733 - ABIE40_RS08665 0.57 +0.3 1,674,733 - ABIE40_RS08665 0.57 -0.3 1,674,740 + ABIE40_RS08665 0.58 +0.9 1,674,746 + ABIE40_RS08665 0.59 -0.9 1,674,776 + ABIE40_RS08665 0.62 -1.8 1,674,777 - ABIE40_RS08665 0.62 +0.4 1,674,777 - ABIE40_RS08665 0.62 -0.9 1,674,789 + ABIE40_RS08665 0.63 -0.6 1,674,802 + ABIE40_RS08665 0.65 +0.6 1,674,802 + ABIE40_RS08665 0.65 +0.1 1,674,802 + ABIE40_RS08665 0.65 -0.6 1,674,802 + ABIE40_RS08665 0.65 +0.3 1,674,802 + ABIE40_RS08665 0.65 -2.0 1,674,802 + ABIE40_RS08665 0.65 -0.4 1,674,803 - ABIE40_RS08665 0.65 +0.9 1,674,803 - ABIE40_RS08665 0.65 -0.1 1,674,803 - ABIE40_RS08665 0.65 -1.5 1,674,803 - ABIE40_RS08665 0.65 -1.9 1,674,806 + ABIE40_RS08665 0.65 -2.4 1,674,806 + ABIE40_RS08665 0.65 +0.9 1,674,806 + ABIE40_RS08665 0.65 +0.7 1,674,807 - ABIE40_RS08665 0.65 -1.4 1,674,807 - ABIE40_RS08665 0.65 -2.4 1,674,931 - ABIE40_RS08665 0.79 +0.6 1,674,933 - ABIE40_RS08665 0.80 -1.4 1,674,973 + ABIE40_RS08665 0.84 +0.9 1,675,002 + ABIE40_RS08665 0.87 -0.1 1,675,002 + ABIE40_RS08665 0.87 -0.2 1,675,153 + -0.6 1,675,153 + -0.3 1,675,153 + +1.4 1,675,154 - -0.0 1,675,154 - +0.8 1,675,154 - -2.3 1,675,154 - +0.8 1,675,155 + -3.3 1,675,198 + -2.6 1,675,198 + +0.5 1,675,198 + -0.9 1,675,199 - +0.2 1,675,288 + ABIE40_RS08670 0.16 -0.3 1,675,288 + ABIE40_RS08670 0.16 +4.5 1,675,359 + ABIE40_RS08670 0.23 -0.8 1,675,360 - ABIE40_RS08670 0.23 +0.1 1,675,360 - ABIE40_RS08670 0.23 -0.1 1,675,360 - ABIE40_RS08670 0.23 +0.4 1,675,432 + ABIE40_RS08670 0.30 +0.4 1,675,432 + ABIE40_RS08670 0.30 -0.3 1,675,433 - ABIE40_RS08670 0.30 +1.1 1,675,433 - ABIE40_RS08670 0.30 +0.0 1,675,495 + ABIE40_RS08670 0.36 -1.1 1,675,495 + ABIE40_RS08670 0.36 -0.3 1,675,495 + ABIE40_RS08670 0.36 +1.6 1,675,496 - ABIE40_RS08670 0.36 -1.6 1,675,496 - ABIE40_RS08670 0.36 -0.9 1,675,496 - ABIE40_RS08670 0.36 -0.3 1,675,496 - ABIE40_RS08670 0.36 +0.1 1,675,496 - ABIE40_RS08670 0.36 +0.6 1,675,606 + ABIE40_RS08670 0.46 +0.9 1,675,607 - ABIE40_RS08670 0.46 -0.6 1,675,607 - ABIE40_RS08670 0.46 -2.0 1,675,645 + ABIE40_RS08670 0.50 -1.1 1,675,645 + ABIE40_RS08670 0.50 +0.6 1,675,645 + ABIE40_RS08670 0.50 +0.5 1,675,646 - ABIE40_RS08670 0.50 +0.5 1,675,646 - ABIE40_RS08670 0.50 -0.8 1,675,717 + ABIE40_RS08670 0.57 -1.0 1,675,762 + ABIE40_RS08670 0.61 +1.3 1,675,762 + ABIE40_RS08670 0.61 -1.6 1,675,762 + ABIE40_RS08670 0.61 -0.4 1,675,762 + ABIE40_RS08670 0.61 -0.3 1,675,763 - ABIE40_RS08670 0.61 +0.1 1,675,763 - ABIE40_RS08670 0.61 -2.4 1,675,763 - ABIE40_RS08670 0.61 -0.5 1,675,871 + ABIE40_RS08670 0.71 -2.5 1,675,871 + ABIE40_RS08670 0.71 -0.9 1,675,872 - ABIE40_RS08670 0.72 +0.3 1,675,872 - ABIE40_RS08670 0.72 +0.1 1,675,998 - ABIE40_RS08670 0.84 +0.4 1,676,065 + ABIE40_RS08670 0.90 -2.2 1,676,065 + ABIE40_RS08670 0.90 -0.5 1,676,066 - -1.6 1,676,066 - +1.3 1,676,214 + -0.0 1,676,214 + +0.0 1,676,215 - +0.7 1,676,215 - -0.5 1,676,215 - +0.4 1,676,303 - ABIE40_RS08675 0.20 +1.0 1,676,345 - ABIE40_RS08675 0.27 -2.0 1,676,346 + ABIE40_RS08675 0.27 +0.5 1,676,346 + ABIE40_RS08675 0.27 -1.2 1,676,346 + ABIE40_RS08675 0.27 -0.4 1,676,346 + ABIE40_RS08675 0.27 +1.4 1,676,346 + ABIE40_RS08675 0.27 +0.1 1,676,364 + ABIE40_RS08675 0.30 -0.2 1,676,365 - ABIE40_RS08675 0.31 +0.0 1,676,365 - ABIE40_RS08675 0.31 +0.5 1,676,377 + ABIE40_RS08675 0.33 -0.2 1,676,377 + ABIE40_RS08675 0.33 -0.5 1,676,685 + ABIE40_RS08675 0.87 -0.9 1,676,685 + ABIE40_RS08675 0.87 +0.3 1,676,769 + -0.4 1,676,770 - +0.9 1,676,770 - +0.4 1,676,770 - -1.2 1,676,830 + -0.4 1,676,830 + +0.1 1,676,830 + +0.2 1,676,830 + -0.0 1,676,830 + -1.7 1,676,831 - -1.7 1,676,831 - -0.7 1,676,831 - -0.9 1,676,831 - -0.1 1,676,831 - -0.3 1,676,832 + +0.4 1,676,833 - +0.4 1,676,908 - +0.3 1,676,908 - +0.3 1,677,000 + ABIE40_RS08680 0.12 -0.4 1,677,001 - ABIE40_RS08680 0.12 -0.3 1,677,036 + ABIE40_RS08680 0.14 -0.0 1,677,036 + ABIE40_RS08680 0.14 -0.0 1,677,036 + ABIE40_RS08680 0.14 -0.2 1,677,037 - ABIE40_RS08680 0.14 -0.9 1,677,046 + ABIE40_RS08680 0.14 -2.6 1,677,046 + ABIE40_RS08680 0.14 -0.7 1,677,046 + ABIE40_RS08680 0.14 -0.1 1,677,046 + ABIE40_RS08680 0.14 -0.6 1,677,096 + ABIE40_RS08680 0.17 -0.3 1,677,096 + ABIE40_RS08680 0.17 +1.2 1,677,096 + ABIE40_RS08680 0.17 -0.0 1,677,096 + ABIE40_RS08680 0.17 -1.1 1,677,097 - ABIE40_RS08680 0.17 +0.6 1,677,166 - ABIE40_RS08680 0.20 -0.3
Or see this region's nucleotide sequence