Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS03490 and ABIE40_RS03495 are separated by 196 nucleotides ABIE40_RS03495 and ABIE40_RS03500 are separated by 3 nucleotides ABIE40_RS03500 and ABIE40_RS03505 are separated by 203 nucleotides ABIE40_RS03505 and ABIE40_RS03510 are separated by 86 nucleotides
ABIE40_RS03490: ABIE40_RS03490 - dienelactone hydrolase family protein, at 692,978 to 693,853
_RS03490
ABIE40_RS03495: ABIE40_RS03495 - TetR family transcriptional regulator, at 694,050 to 694,679
_RS03495
ABIE40_RS03500: ABIE40_RS03500 - LysR substrate-binding domain-containing protein, at 694,683 to 695,639
_RS03500
ABIE40_RS03505: ABIE40_RS03505 - DUF1127 domain-containing protein, at 695,843 to 696,139
_RS03505
ABIE40_RS03510: ABIE40_RS03510 - DUF937 domain-containing protein, at 696,226 to 697,089
_RS03510
Position (kb)
694
695
696 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 693.689 kb on + strand, within ABIE40_RS03490 at 693.690 kb on - strand, within ABIE40_RS03490 at 693.737 kb on + strand, within ABIE40_RS03490 at 693.737 kb on + strand, within ABIE40_RS03490 at 693.738 kb on - strand, within ABIE40_RS03490 at 693.769 kb on + strand at 693.769 kb on + strand at 693.785 kb on + strand at 693.786 kb on - strand at 693.883 kb on + strand at 693.960 kb on + strand at 694.033 kb on - strand at 694.057 kb on + strand at 694.057 kb on + strand at 694.058 kb on - strand at 694.058 kb on - strand at 694.058 kb on - strand at 694.228 kb on - strand, within ABIE40_RS03495 at 694.228 kb on - strand, within ABIE40_RS03495 at 694.234 kb on - strand, within ABIE40_RS03495 at 694.234 kb on - strand, within ABIE40_RS03495 at 694.374 kb on + strand, within ABIE40_RS03495 at 694.374 kb on + strand, within ABIE40_RS03495 at 694.375 kb on - strand, within ABIE40_RS03495 at 694.377 kb on + strand, within ABIE40_RS03495 at 694.377 kb on + strand, within ABIE40_RS03495 at 694.377 kb on + strand, within ABIE40_RS03495 at 694.377 kb on + strand, within ABIE40_RS03495 at 694.377 kb on + strand, within ABIE40_RS03495 at 694.378 kb on - strand, within ABIE40_RS03495 at 694.378 kb on - strand, within ABIE40_RS03495 at 694.378 kb on - strand, within ABIE40_RS03495 at 694.378 kb on - strand, within ABIE40_RS03495 at 694.378 kb on - strand, within ABIE40_RS03495 at 694.387 kb on - strand, within ABIE40_RS03495 at 694.404 kb on + strand, within ABIE40_RS03495 at 694.404 kb on + strand, within ABIE40_RS03495 at 694.405 kb on - strand, within ABIE40_RS03495 at 694.458 kb on - strand, within ABIE40_RS03495 at 694.461 kb on + strand, within ABIE40_RS03495 at 694.484 kb on + strand, within ABIE40_RS03495 at 694.485 kb on - strand, within ABIE40_RS03495 at 694.551 kb on + strand, within ABIE40_RS03495 at 694.551 kb on + strand, within ABIE40_RS03495 at 694.551 kb on + strand, within ABIE40_RS03495 at 694.551 kb on + strand, within ABIE40_RS03495 at 694.552 kb on - strand, within ABIE40_RS03495 at 694.552 kb on - strand, within ABIE40_RS03495 at 694.552 kb on - strand, within ABIE40_RS03495 at 694.552 kb on - strand, within ABIE40_RS03495 at 694.667 kb on - strand at 694.672 kb on + strand at 694.672 kb on + strand at 694.672 kb on + strand at 694.672 kb on + strand at 694.672 kb on + strand at 694.672 kb on + strand at 694.672 kb on + strand at 694.673 kb on - strand at 694.673 kb on - strand at 694.673 kb on - strand at 694.673 kb on - strand at 694.673 kb on - strand at 694.684 kb on + strand at 694.684 kb on + strand at 694.684 kb on + strand at 694.735 kb on + strand at 694.736 kb on - strand at 694.736 kb on - strand at 694.808 kb on + strand, within ABIE40_RS03500 at 694.809 kb on - strand, within ABIE40_RS03500 at 694.809 kb on - strand, within ABIE40_RS03500 at 694.809 kb on - strand, within ABIE40_RS03500 at 694.809 kb on - strand, within ABIE40_RS03500 at 694.809 kb on - strand, within ABIE40_RS03500 at 694.919 kb on + strand, within ABIE40_RS03500 at 694.919 kb on + strand, within ABIE40_RS03500 at 695.189 kb on + strand, within ABIE40_RS03500 at 695.190 kb on - strand, within ABIE40_RS03500 at 695.190 kb on - strand, within ABIE40_RS03500 at 695.266 kb on + strand, within ABIE40_RS03500 at 695.266 kb on + strand, within ABIE40_RS03500 at 695.266 kb on + strand, within ABIE40_RS03500 at 695.267 kb on - strand, within ABIE40_RS03500 at 695.267 kb on - strand, within ABIE40_RS03500 at 695.267 kb on - strand, within ABIE40_RS03500 at 695.267 kb on - strand, within ABIE40_RS03500 at 695.267 kb on - strand, within ABIE40_RS03500 at 695.267 kb on - strand, within ABIE40_RS03500 at 695.267 kb on - strand, within ABIE40_RS03500 at 695.320 kb on + strand, within ABIE40_RS03500 at 695.320 kb on + strand, within ABIE40_RS03500 at 695.320 kb on + strand, within ABIE40_RS03500 at 695.320 kb on + strand, within ABIE40_RS03500 at 695.321 kb on - strand, within ABIE40_RS03500 at 695.321 kb on - strand, within ABIE40_RS03500 at 695.321 kb on - strand, within ABIE40_RS03500 at 695.321 kb on - strand, within ABIE40_RS03500 at 695.321 kb on - strand, within ABIE40_RS03500 at 695.332 kb on + strand, within ABIE40_RS03500 at 695.333 kb on - strand, within ABIE40_RS03500 at 695.428 kb on + strand, within ABIE40_RS03500 at 695.428 kb on + strand, within ABIE40_RS03500 at 695.429 kb on - strand, within ABIE40_RS03500 at 695.551 kb on + strand at 695.551 kb on + strand at 695.551 kb on + strand at 695.551 kb on + strand at 695.551 kb on + strand at 695.552 kb on - strand at 695.649 kb on + strand at 695.651 kb on + strand at 695.865 kb on + strand at 695.865 kb on + strand at 695.865 kb on + strand at 695.866 kb on - strand at 695.993 kb on + strand, within ABIE40_RS03505 at 695.993 kb on + strand, within ABIE40_RS03505 at 695.994 kb on - strand, within ABIE40_RS03505 at 696.128 kb on + strand at 696.128 kb on + strand at 696.128 kb on + strand at 696.128 kb on + strand at 696.129 kb on - strand at 696.129 kb on - strand at 696.132 kb on + strand at 696.132 kb on + strand at 696.132 kb on + strand at 696.132 kb on + strand at 696.132 kb on + strand at 696.133 kb on - strand at 696.133 kb on - strand at 696.133 kb on - strand at 696.137 kb on + strand at 696.137 kb on + strand at 696.138 kb on - strand at 696.138 kb on - strand at 696.138 kb on - strand at 696.167 kb on - strand at 696.174 kb on - strand at 696.187 kb on - strand at 696.296 kb on + strand at 696.350 kb on + strand, within ABIE40_RS03510 at 696.350 kb on + strand, within ABIE40_RS03510 at 696.351 kb on - strand, within ABIE40_RS03510 at 696.351 kb on - strand, within ABIE40_RS03510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 693,689 + ABIE40_RS03490 0.81 -1.6 693,690 - ABIE40_RS03490 0.81 +0.3 693,737 + ABIE40_RS03490 0.87 +0.6 693,737 + ABIE40_RS03490 0.87 +0.0 693,738 - ABIE40_RS03490 0.87 +1.1 693,769 + +2.8 693,769 + +0.8 693,785 + +1.8 693,786 - -1.2 693,883 + -0.0 693,960 + +0.4 694,033 - -0.7 694,057 + -2.4 694,057 + -0.1 694,058 - -1.2 694,058 - +0.8 694,058 - -1.7 694,228 - ABIE40_RS03495 0.28 -1.7 694,228 - ABIE40_RS03495 0.28 -2.8 694,234 - ABIE40_RS03495 0.29 -1.0 694,234 - ABIE40_RS03495 0.29 -0.8 694,374 + ABIE40_RS03495 0.51 -1.1 694,374 + ABIE40_RS03495 0.51 -1.8 694,375 - ABIE40_RS03495 0.52 +1.2 694,377 + ABIE40_RS03495 0.52 -1.4 694,377 + ABIE40_RS03495 0.52 -2.2 694,377 + ABIE40_RS03495 0.52 -0.8 694,377 + ABIE40_RS03495 0.52 -0.0 694,377 + ABIE40_RS03495 0.52 -2.6 694,378 - ABIE40_RS03495 0.52 -1.1 694,378 - ABIE40_RS03495 0.52 -2.5 694,378 - ABIE40_RS03495 0.52 +1.2 694,378 - ABIE40_RS03495 0.52 -0.5 694,378 - ABIE40_RS03495 0.52 -0.7 694,387 - ABIE40_RS03495 0.53 -1.5 694,404 + ABIE40_RS03495 0.56 -1.1 694,404 + ABIE40_RS03495 0.56 +0.2 694,405 - ABIE40_RS03495 0.56 -0.5 694,458 - ABIE40_RS03495 0.65 -0.4 694,461 + ABIE40_RS03495 0.65 -1.6 694,484 + ABIE40_RS03495 0.69 -0.3 694,485 - ABIE40_RS03495 0.69 -0.2 694,551 + ABIE40_RS03495 0.80 -2.7 694,551 + ABIE40_RS03495 0.80 +0.2 694,551 + ABIE40_RS03495 0.80 -2.5 694,551 + ABIE40_RS03495 0.80 +0.0 694,552 - ABIE40_RS03495 0.80 -1.0 694,552 - ABIE40_RS03495 0.80 -1.1 694,552 - ABIE40_RS03495 0.80 -0.9 694,552 - ABIE40_RS03495 0.80 -0.8 694,667 - +1.8 694,672 + -0.9 694,672 + +0.5 694,672 + -0.8 694,672 + +0.7 694,672 + +0.2 694,672 + -1.0 694,672 + +0.5 694,673 - +0.0 694,673 - +1.1 694,673 - -1.0 694,673 - -0.8 694,673 - +1.4 694,684 + -0.5 694,684 + -1.7 694,684 + +0.5 694,735 + -1.8 694,736 - +0.6 694,736 - -0.2 694,808 + ABIE40_RS03500 0.13 -1.4 694,809 - ABIE40_RS03500 0.13 -0.6 694,809 - ABIE40_RS03500 0.13 -1.5 694,809 - ABIE40_RS03500 0.13 -0.4 694,809 - ABIE40_RS03500 0.13 +0.3 694,809 - ABIE40_RS03500 0.13 -0.6 694,919 + ABIE40_RS03500 0.25 -2.2 694,919 + ABIE40_RS03500 0.25 -1.1 695,189 + ABIE40_RS03500 0.53 -0.9 695,190 - ABIE40_RS03500 0.53 -0.6 695,190 - ABIE40_RS03500 0.53 -0.7 695,266 + ABIE40_RS03500 0.61 -1.5 695,266 + ABIE40_RS03500 0.61 +1.3 695,266 + ABIE40_RS03500 0.61 -1.5 695,267 - ABIE40_RS03500 0.61 -0.5 695,267 - ABIE40_RS03500 0.61 -0.6 695,267 - ABIE40_RS03500 0.61 -0.9 695,267 - ABIE40_RS03500 0.61 -0.8 695,267 - ABIE40_RS03500 0.61 -0.6 695,267 - ABIE40_RS03500 0.61 -0.7 695,267 - ABIE40_RS03500 0.61 -0.5 695,320 + ABIE40_RS03500 0.67 -0.2 695,320 + ABIE40_RS03500 0.67 -0.0 695,320 + ABIE40_RS03500 0.67 +0.1 695,320 + ABIE40_RS03500 0.67 -0.1 695,321 - ABIE40_RS03500 0.67 -2.1 695,321 - ABIE40_RS03500 0.67 -0.4 695,321 - ABIE40_RS03500 0.67 -1.0 695,321 - ABIE40_RS03500 0.67 -0.4 695,321 - ABIE40_RS03500 0.67 -1.4 695,332 + ABIE40_RS03500 0.68 -1.0 695,333 - ABIE40_RS03500 0.68 -0.5 695,428 + ABIE40_RS03500 0.78 +0.4 695,428 + ABIE40_RS03500 0.78 -0.5 695,429 - ABIE40_RS03500 0.78 -0.7 695,551 + -2.1 695,551 + -1.4 695,551 + -0.1 695,551 + -2.6 695,551 + +1.8 695,552 - -1.0 695,649 + -0.5 695,651 + -1.7 695,865 + -0.6 695,865 + +0.3 695,865 + +0.6 695,866 - -1.5 695,993 + ABIE40_RS03505 0.51 -0.2 695,993 + ABIE40_RS03505 0.51 -0.2 695,994 - ABIE40_RS03505 0.51 -0.9 696,128 + +1.0 696,128 + +0.4 696,128 + -0.3 696,128 + +0.0 696,129 - -0.4 696,129 - -0.1 696,132 + +0.6 696,132 + +0.4 696,132 + +0.2 696,132 + +0.9 696,132 + +0.7 696,133 - -1.8 696,133 - -1.3 696,133 - -0.5 696,137 + +1.1 696,137 + +0.0 696,138 - -2.2 696,138 - +0.5 696,138 - -1.9 696,167 - -1.3 696,174 - -0.4 696,187 - -0.1 696,296 + -0.7 696,350 + ABIE40_RS03510 0.14 -0.2 696,350 + ABIE40_RS03510 0.14 +0.3 696,351 - ABIE40_RS03510 0.14 -1.3 696,351 - ABIE40_RS03510 0.14 -0.5
Or see this region's nucleotide sequence