Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS02115 and ABIE40_RS02120 are separated by 34 nucleotides ABIE40_RS02120 and ABIE40_RS02125 are separated by 92 nucleotides ABIE40_RS02125 and ABIE40_RS02130 overlap by 4 nucleotides
ABIE40_RS02115: ABIE40_RS02115 - VOC family protein, at 415,522 to 416,058
_RS02115
ABIE40_RS02120: ABIE40_RS02120 - anthrone oxygenase family protein, at 416,093 to 416,575
_RS02120
ABIE40_RS02125: ABIE40_RS02125 - TetR family transcriptional regulator, at 416,668 to 417,318
_RS02125
ABIE40_RS02130: ABIE40_RS02130 - GGDEF domain-containing protein, at 417,315 to 418,217
_RS02130
Position (kb)
416
417
418 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 415.762 kb on + strand, within ABIE40_RS02115 at 415.762 kb on + strand, within ABIE40_RS02115 at 415.763 kb on - strand, within ABIE40_RS02115 at 415.763 kb on - strand, within ABIE40_RS02115 at 415.825 kb on + strand, within ABIE40_RS02115 at 415.826 kb on - strand, within ABIE40_RS02115 at 415.826 kb on - strand, within ABIE40_RS02115 at 415.826 kb on - strand, within ABIE40_RS02115 at 415.826 kb on - strand, within ABIE40_RS02115 at 415.826 kb on - strand, within ABIE40_RS02115 at 415.826 kb on - strand, within ABIE40_RS02115 at 415.826 kb on - strand, within ABIE40_RS02115 at 415.957 kb on + strand, within ABIE40_RS02115 at 415.963 kb on + strand, within ABIE40_RS02115 at 415.963 kb on + strand, within ABIE40_RS02115 at 415.964 kb on - strand, within ABIE40_RS02115 at 415.964 kb on - strand, within ABIE40_RS02115 at 416.096 kb on + strand at 416.105 kb on - strand at 416.184 kb on + strand, within ABIE40_RS02120 at 416.185 kb on - strand, within ABIE40_RS02120 at 416.290 kb on + strand, within ABIE40_RS02120 at 416.290 kb on + strand, within ABIE40_RS02120 at 416.291 kb on - strand, within ABIE40_RS02120 at 416.291 kb on - strand, within ABIE40_RS02120 at 416.292 kb on + strand, within ABIE40_RS02120 at 416.292 kb on + strand, within ABIE40_RS02120 at 416.292 kb on + strand, within ABIE40_RS02120 at 416.292 kb on + strand, within ABIE40_RS02120 at 416.292 kb on + strand, within ABIE40_RS02120 at 416.293 kb on - strand, within ABIE40_RS02120 at 416.293 kb on - strand, within ABIE40_RS02120 at 416.293 kb on - strand, within ABIE40_RS02120 at 416.293 kb on - strand, within ABIE40_RS02120 at 416.510 kb on + strand, within ABIE40_RS02120 at 416.511 kb on - strand, within ABIE40_RS02120 at 416.730 kb on + strand at 416.731 kb on - strand at 416.869 kb on + strand, within ABIE40_RS02125 at 416.869 kb on + strand, within ABIE40_RS02125 at 416.869 kb on + strand, within ABIE40_RS02125 at 416.870 kb on - strand, within ABIE40_RS02125 at 416.870 kb on - strand, within ABIE40_RS02125 at 416.870 kb on - strand, within ABIE40_RS02125 at 416.870 kb on - strand, within ABIE40_RS02125 at 416.870 kb on - strand, within ABIE40_RS02125 at 416.875 kb on + strand, within ABIE40_RS02125 at 416.967 kb on + strand, within ABIE40_RS02125 at 417.016 kb on + strand, within ABIE40_RS02125 at 417.016 kb on + strand, within ABIE40_RS02125 at 417.016 kb on + strand, within ABIE40_RS02125 at 417.016 kb on + strand, within ABIE40_RS02125 at 417.016 kb on + strand, within ABIE40_RS02125 at 417.016 kb on + strand, within ABIE40_RS02125 at 417.016 kb on + strand, within ABIE40_RS02125 at 417.017 kb on - strand, within ABIE40_RS02125 at 417.017 kb on - strand, within ABIE40_RS02125 at 417.055 kb on - strand, within ABIE40_RS02125 at 417.277 kb on + strand at 417.278 kb on - strand at 417.278 kb on - strand at 417.316 kb on + strand at 417.316 kb on + strand at 417.394 kb on + strand at 417.394 kb on + strand at 417.394 kb on + strand at 417.395 kb on - strand at 417.395 kb on - strand at 417.457 kb on + strand, within ABIE40_RS02130 at 417.619 kb on + strand, within ABIE40_RS02130 at 417.620 kb on - strand, within ABIE40_RS02130 at 417.620 kb on - strand, within ABIE40_RS02130 at 417.620 kb on - strand, within ABIE40_RS02130 at 417.961 kb on + strand, within ABIE40_RS02130 at 417.962 kb on - strand, within ABIE40_RS02130 at 417.962 kb on - strand, within ABIE40_RS02130 at 418.105 kb on + strand, within ABIE40_RS02130 at 418.105 kb on + strand, within ABIE40_RS02130 at 418.105 kb on + strand, within ABIE40_RS02130 at 418.106 kb on - strand, within ABIE40_RS02130 at 418.106 kb on - strand, within ABIE40_RS02130 at 418.130 kb on + strand at 418.149 kb on + strand at 418.149 kb on + strand at 418.149 kb on + strand at 418.149 kb on + strand at 418.149 kb on + strand at 418.149 kb on + strand at 418.150 kb on - strand at 418.150 kb on - strand at 418.283 kb on + strand at 418.284 kb on - strand at 418.284 kb on - strand at 418.284 kb on - strand at 418.284 kb on - strand at 418.295 kb on + strand at 418.296 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 415,762 + ABIE40_RS02115 0.45 +0.4 415,762 + ABIE40_RS02115 0.45 -3.3 415,763 - ABIE40_RS02115 0.45 +0.5 415,763 - ABIE40_RS02115 0.45 -0.9 415,825 + ABIE40_RS02115 0.56 -1.1 415,826 - ABIE40_RS02115 0.57 -0.8 415,826 - ABIE40_RS02115 0.57 -1.6 415,826 - ABIE40_RS02115 0.57 -0.9 415,826 - ABIE40_RS02115 0.57 +0.0 415,826 - ABIE40_RS02115 0.57 -1.5 415,826 - ABIE40_RS02115 0.57 +1.2 415,826 - ABIE40_RS02115 0.57 -1.1 415,957 + ABIE40_RS02115 0.81 +0.2 415,963 + ABIE40_RS02115 0.82 +2.3 415,963 + ABIE40_RS02115 0.82 -3.3 415,964 - ABIE40_RS02115 0.82 -1.1 415,964 - ABIE40_RS02115 0.82 -0.6 416,096 + +0.1 416,105 - +0.5 416,184 + ABIE40_RS02120 0.19 -1.3 416,185 - ABIE40_RS02120 0.19 -0.3 416,290 + ABIE40_RS02120 0.41 +0.5 416,290 + ABIE40_RS02120 0.41 -0.7 416,291 - ABIE40_RS02120 0.41 -2.5 416,291 - ABIE40_RS02120 0.41 -1.5 416,292 + ABIE40_RS02120 0.41 +2.5 416,292 + ABIE40_RS02120 0.41 -0.3 416,292 + ABIE40_RS02120 0.41 -0.4 416,292 + ABIE40_RS02120 0.41 -0.7 416,292 + ABIE40_RS02120 0.41 -0.8 416,293 - ABIE40_RS02120 0.41 +0.3 416,293 - ABIE40_RS02120 0.41 +1.4 416,293 - ABIE40_RS02120 0.41 -0.2 416,293 - ABIE40_RS02120 0.41 +0.1 416,510 + ABIE40_RS02120 0.86 -1.0 416,511 - ABIE40_RS02120 0.87 -0.1 416,730 + -0.3 416,731 - -1.2 416,869 + ABIE40_RS02125 0.31 +0.1 416,869 + ABIE40_RS02125 0.31 -0.9 416,869 + ABIE40_RS02125 0.31 -0.4 416,870 - ABIE40_RS02125 0.31 -1.6 416,870 - ABIE40_RS02125 0.31 -0.1 416,870 - ABIE40_RS02125 0.31 -0.2 416,870 - ABIE40_RS02125 0.31 +1.2 416,870 - ABIE40_RS02125 0.31 -0.9 416,875 + ABIE40_RS02125 0.32 -0.6 416,967 + ABIE40_RS02125 0.46 +0.7 417,016 + ABIE40_RS02125 0.53 +0.9 417,016 + ABIE40_RS02125 0.53 -1.3 417,016 + ABIE40_RS02125 0.53 -0.2 417,016 + ABIE40_RS02125 0.53 -0.2 417,016 + ABIE40_RS02125 0.53 -0.4 417,016 + ABIE40_RS02125 0.53 -1.3 417,016 + ABIE40_RS02125 0.53 -2.6 417,017 - ABIE40_RS02125 0.54 -2.2 417,017 - ABIE40_RS02125 0.54 -1.1 417,055 - ABIE40_RS02125 0.59 -2.3 417,277 + -1.5 417,278 - -1.6 417,278 - -3.8 417,316 + +0.3 417,316 + -2.3 417,394 + +0.4 417,394 + -0.1 417,394 + -0.5 417,395 - -0.6 417,395 - -2.2 417,457 + ABIE40_RS02130 0.16 +0.2 417,619 + ABIE40_RS02130 0.34 -0.2 417,620 - ABIE40_RS02130 0.34 +0.3 417,620 - ABIE40_RS02130 0.34 +0.6 417,620 - ABIE40_RS02130 0.34 +0.5 417,961 + ABIE40_RS02130 0.72 -0.7 417,962 - ABIE40_RS02130 0.72 +1.5 417,962 - ABIE40_RS02130 0.72 +0.1 418,105 + ABIE40_RS02130 0.87 -0.2 418,105 + ABIE40_RS02130 0.87 +0.7 418,105 + ABIE40_RS02130 0.87 -0.3 418,106 - ABIE40_RS02130 0.88 -0.1 418,106 - ABIE40_RS02130 0.88 +0.2 418,130 + -2.2 418,149 + +0.7 418,149 + -3.1 418,149 + +1.7 418,149 + +0.7 418,149 + +0.7 418,149 + -0.6 418,150 - -0.8 418,150 - -2.2 418,283 + +4.7 418,284 - +0.4 418,284 - -0.3 418,284 - -0.0 418,284 - -0.4 418,295 + -2.8 418,296 - +0.1
Or see this region's nucleotide sequence