Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS29515 and ABIE40_RS29520 are separated by 290 nucleotides ABIE40_RS29520 and ABIE40_RS29525 are separated by 342 nucleotides ABIE40_RS29525 and ABIE40_RS29530 are separated by 787 nucleotides
ABIE40_RS29515: ABIE40_RS29515 - hypothetical protein, at 595,175 to 595,798
_RS29515
ABIE40_RS29520: ABIE40_RS29520 - hypothetical protein, at 596,089 to 596,334
_RS29520
ABIE40_RS29525: ABIE40_RS29525 - DNA ligase D, at 596,677 to 599,340
_RS29525
ABIE40_RS29530: ABIE40_RS29530 - hypothetical protein, at 600,128 to 600,865
_RS29530
Position (kb)
596
597
598
599
600 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 595.706 kb on - strand, within ABIE40_RS29515 at 595.817 kb on + strand at 595.818 kb on - strand at 595.851 kb on - strand at 595.851 kb on - strand at 595.873 kb on + strand at 595.874 kb on - strand at 595.876 kb on + strand at 595.877 kb on - strand at 595.877 kb on - strand at 595.877 kb on - strand at 595.895 kb on + strand at 595.903 kb on + strand at 595.903 kb on + strand at 595.904 kb on - strand at 595.933 kb on + strand at 596.027 kb on + strand at 596.090 kb on + strand at 596.090 kb on + strand at 596.090 kb on + strand at 596.091 kb on - strand at 596.091 kb on - strand at 596.091 kb on - strand at 596.091 kb on - strand at 596.091 kb on - strand at 596.123 kb on + strand, within ABIE40_RS29520 at 596.123 kb on + strand, within ABIE40_RS29520 at 596.123 kb on + strand, within ABIE40_RS29520 at 596.124 kb on - strand, within ABIE40_RS29520 at 596.124 kb on - strand, within ABIE40_RS29520 at 596.134 kb on + strand, within ABIE40_RS29520 at 596.134 kb on + strand, within ABIE40_RS29520 at 596.135 kb on - strand, within ABIE40_RS29520 at 596.135 kb on - strand, within ABIE40_RS29520 at 596.185 kb on + strand, within ABIE40_RS29520 at 596.186 kb on - strand, within ABIE40_RS29520 at 596.215 kb on - strand, within ABIE40_RS29520 at 596.380 kb on + strand at 596.400 kb on - strand at 596.465 kb on - strand at 596.465 kb on - strand at 596.659 kb on + strand at 596.659 kb on + strand at 596.659 kb on + strand at 596.659 kb on + strand at 596.659 kb on + strand at 596.659 kb on + strand at 596.659 kb on + strand at 596.659 kb on + strand at 596.659 kb on + strand at 596.660 kb on - strand at 596.660 kb on - strand at 596.660 kb on - strand at 596.660 kb on - strand at 596.660 kb on - strand at 596.660 kb on - strand at 596.673 kb on + strand at 596.673 kb on + strand at 596.673 kb on + strand at 596.674 kb on - strand at 596.674 kb on - strand at 596.747 kb on + strand at 596.748 kb on - strand at 596.748 kb on - strand at 596.748 kb on - strand at 596.748 kb on - strand at 596.925 kb on - strand at 596.975 kb on + strand, within ABIE40_RS29525 at 596.975 kb on + strand, within ABIE40_RS29525 at 596.975 kb on + strand, within ABIE40_RS29525 at 596.976 kb on - strand, within ABIE40_RS29525 at 597.188 kb on - strand, within ABIE40_RS29525 at 597.188 kb on - strand, within ABIE40_RS29525 at 597.188 kb on - strand, within ABIE40_RS29525 at 597.188 kb on - strand, within ABIE40_RS29525 at 597.188 kb on - strand, within ABIE40_RS29525 at 597.326 kb on + strand, within ABIE40_RS29525 at 597.327 kb on - strand, within ABIE40_RS29525 at 597.457 kb on + strand, within ABIE40_RS29525 at 597.457 kb on + strand, within ABIE40_RS29525 at 597.457 kb on + strand, within ABIE40_RS29525 at 597.457 kb on + strand, within ABIE40_RS29525 at 597.457 kb on + strand, within ABIE40_RS29525 at 597.457 kb on + strand, within ABIE40_RS29525 at 597.457 kb on + strand, within ABIE40_RS29525 at 597.457 kb on + strand, within ABIE40_RS29525 at 597.458 kb on - strand, within ABIE40_RS29525 at 597.458 kb on - strand, within ABIE40_RS29525 at 597.458 kb on - strand, within ABIE40_RS29525 at 597.458 kb on - strand, within ABIE40_RS29525 at 597.459 kb on + strand, within ABIE40_RS29525 at 597.460 kb on - strand, within ABIE40_RS29525 at 597.460 kb on - strand, within ABIE40_RS29525 at 597.460 kb on - strand, within ABIE40_RS29525 at 597.460 kb on - strand, within ABIE40_RS29525 at 597.468 kb on + strand, within ABIE40_RS29525 at 597.491 kb on + strand, within ABIE40_RS29525 at 597.491 kb on + strand, within ABIE40_RS29525 at 597.492 kb on - strand, within ABIE40_RS29525 at 597.492 kb on - strand, within ABIE40_RS29525 at 597.492 kb on - strand, within ABIE40_RS29525 at 597.492 kb on - strand, within ABIE40_RS29525 at 597.492 kb on - strand, within ABIE40_RS29525 at 597.494 kb on + strand, within ABIE40_RS29525 at 597.494 kb on + strand, within ABIE40_RS29525 at 597.495 kb on - strand, within ABIE40_RS29525 at 597.703 kb on - strand, within ABIE40_RS29525 at 597.929 kb on + strand, within ABIE40_RS29525 at 597.929 kb on + strand, within ABIE40_RS29525 at 597.941 kb on + strand, within ABIE40_RS29525 at 597.995 kb on + strand, within ABIE40_RS29525 at 597.995 kb on + strand, within ABIE40_RS29525 at 597.995 kb on + strand, within ABIE40_RS29525 at 597.996 kb on - strand, within ABIE40_RS29525 at 597.996 kb on - strand, within ABIE40_RS29525 at 598.067 kb on + strand, within ABIE40_RS29525 at 598.068 kb on - strand, within ABIE40_RS29525 at 598.283 kb on + strand, within ABIE40_RS29525 at 598.283 kb on + strand, within ABIE40_RS29525 at 598.284 kb on - strand, within ABIE40_RS29525 at 598.425 kb on - strand, within ABIE40_RS29525 at 598.591 kb on + strand, within ABIE40_RS29525 at 598.592 kb on - strand, within ABIE40_RS29525 at 598.638 kb on + strand, within ABIE40_RS29525 at 598.638 kb on + strand, within ABIE40_RS29525 at 598.864 kb on + strand, within ABIE40_RS29525 at 598.865 kb on - strand, within ABIE40_RS29525 at 598.924 kb on - strand, within ABIE40_RS29525 at 599.084 kb on + strand at 599.084 kb on + strand at 599.085 kb on - strand at 599.085 kb on - strand at 599.201 kb on + strand at 599.201 kb on + strand at 599.201 kb on + strand at 599.202 kb on - strand at 599.202 kb on - strand at 599.202 kb on - strand at 599.202 kb on - strand at 599.202 kb on - strand at 599.202 kb on - strand at 599.522 kb on + strand at 599.523 kb on - strand at 599.523 kb on - strand at 599.523 kb on - strand at 599.523 kb on - strand at 599.653 kb on + strand at 599.673 kb on + strand at 599.673 kb on + strand at 599.673 kb on + strand at 599.673 kb on + strand at 599.674 kb on - strand at 599.733 kb on + strand at 599.733 kb on + strand at 599.733 kb on + strand at 599.734 kb on - strand at 599.841 kb on + strand at 599.887 kb on - strand at 599.887 kb on - strand at 599.887 kb on - strand at 599.946 kb on + strand at 599.947 kb on - strand at 599.947 kb on - strand at 599.947 kb on - strand at 599.947 kb on - strand at 600.011 kb on + strand at 600.011 kb on + strand at 600.011 kb on + strand at 600.011 kb on + strand at 600.011 kb on + strand at 600.012 kb on - strand at 600.012 kb on - strand at 600.047 kb on + strand at 600.059 kb on - strand at 600.084 kb on + strand at 600.084 kb on + strand at 600.099 kb on + strand at 600.099 kb on + strand at 600.099 kb on + strand at 600.244 kb on + strand, within ABIE40_RS29530 at 600.245 kb on - strand, within ABIE40_RS29530 at 600.321 kb on + strand, within ABIE40_RS29530 at 600.321 kb on + strand, within ABIE40_RS29530 at 600.322 kb on - strand, within ABIE40_RS29530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 595,706 - ABIE40_RS29515 0.85 +1.3 595,817 + -3.5 595,818 - +1.6 595,851 - -3.3 595,851 - -2.5 595,873 + -3.6 595,874 - -1.3 595,876 + +2.3 595,877 - +0.3 595,877 - -0.9 595,877 - +0.6 595,895 + +1.7 595,903 + -1.4 595,903 + +1.4 595,904 - -0.7 595,933 + -0.6 596,027 + +0.1 596,090 + +0.7 596,090 + +1.0 596,090 + +0.6 596,091 - +0.6 596,091 - -0.7 596,091 - -0.1 596,091 - +1.1 596,091 - -0.9 596,123 + ABIE40_RS29520 0.14 -1.9 596,123 + ABIE40_RS29520 0.14 +0.7 596,123 + ABIE40_RS29520 0.14 +2.3 596,124 - ABIE40_RS29520 0.14 -0.1 596,124 - ABIE40_RS29520 0.14 +1.7 596,134 + ABIE40_RS29520 0.18 -0.1 596,134 + ABIE40_RS29520 0.18 -1.6 596,135 - ABIE40_RS29520 0.19 -0.6 596,135 - ABIE40_RS29520 0.19 -0.7 596,185 + ABIE40_RS29520 0.39 +0.7 596,186 - ABIE40_RS29520 0.39 +1.5 596,215 - ABIE40_RS29520 0.51 +0.3 596,380 + +1.1 596,400 - +1.2 596,465 - +1.4 596,465 - +0.3 596,659 + -0.6 596,659 + -0.1 596,659 + +0.3 596,659 + -2.5 596,659 + -1.8 596,659 + -2.0 596,659 + -2.3 596,659 + +1.1 596,659 + -2.7 596,660 - +0.9 596,660 - -1.5 596,660 - -0.1 596,660 - +0.3 596,660 - +0.5 596,660 - -2.2 596,673 + +1.3 596,673 + -1.8 596,673 + -2.6 596,674 - +0.5 596,674 - +2.3 596,747 + -1.7 596,748 - -1.0 596,748 - +1.1 596,748 - -2.1 596,748 - -0.3 596,925 - -1.8 596,975 + ABIE40_RS29525 0.11 -2.9 596,975 + ABIE40_RS29525 0.11 -1.7 596,975 + ABIE40_RS29525 0.11 +1.7 596,976 - ABIE40_RS29525 0.11 +2.3 597,188 - ABIE40_RS29525 0.19 +1.5 597,188 - ABIE40_RS29525 0.19 -2.0 597,188 - ABIE40_RS29525 0.19 +0.7 597,188 - ABIE40_RS29525 0.19 +4.1 597,188 - ABIE40_RS29525 0.19 -0.1 597,326 + ABIE40_RS29525 0.24 +0.2 597,327 - ABIE40_RS29525 0.24 +2.9 597,457 + ABIE40_RS29525 0.29 +0.3 597,457 + ABIE40_RS29525 0.29 -0.1 597,457 + ABIE40_RS29525 0.29 -1.9 597,457 + ABIE40_RS29525 0.29 +1.7 597,457 + ABIE40_RS29525 0.29 -0.1 597,457 + ABIE40_RS29525 0.29 +3.0 597,457 + ABIE40_RS29525 0.29 +3.6 597,457 + ABIE40_RS29525 0.29 -0.7 597,458 - ABIE40_RS29525 0.29 +1.4 597,458 - ABIE40_RS29525 0.29 -0.3 597,458 - ABIE40_RS29525 0.29 -0.5 597,458 - ABIE40_RS29525 0.29 -0.8 597,459 + ABIE40_RS29525 0.29 +0.7 597,460 - ABIE40_RS29525 0.29 -0.1 597,460 - ABIE40_RS29525 0.29 -0.3 597,460 - ABIE40_RS29525 0.29 +0.5 597,460 - ABIE40_RS29525 0.29 -1.8 597,468 + ABIE40_RS29525 0.30 -0.0 597,491 + ABIE40_RS29525 0.31 +0.4 597,491 + ABIE40_RS29525 0.31 +2.3 597,492 - ABIE40_RS29525 0.31 +1.4 597,492 - ABIE40_RS29525 0.31 -0.2 597,492 - ABIE40_RS29525 0.31 +0.3 597,492 - ABIE40_RS29525 0.31 +0.4 597,492 - ABIE40_RS29525 0.31 -0.5 597,494 + ABIE40_RS29525 0.31 -2.9 597,494 + ABIE40_RS29525 0.31 +0.8 597,495 - ABIE40_RS29525 0.31 +2.3 597,703 - ABIE40_RS29525 0.39 +1.0 597,929 + ABIE40_RS29525 0.47 -1.7 597,929 + ABIE40_RS29525 0.47 -0.7 597,941 + ABIE40_RS29525 0.47 +1.0 597,995 + ABIE40_RS29525 0.49 -2.4 597,995 + ABIE40_RS29525 0.49 -0.7 597,995 + ABIE40_RS29525 0.49 +0.7 597,996 - ABIE40_RS29525 0.50 -0.9 597,996 - ABIE40_RS29525 0.50 -1.3 598,067 + ABIE40_RS29525 0.52 -1.2 598,068 - ABIE40_RS29525 0.52 +1.3 598,283 + ABIE40_RS29525 0.60 +1.1 598,283 + ABIE40_RS29525 0.60 -3.1 598,284 - ABIE40_RS29525 0.60 +0.3 598,425 - ABIE40_RS29525 0.66 +0.9 598,591 + ABIE40_RS29525 0.72 +0.7 598,592 - ABIE40_RS29525 0.72 +0.6 598,638 + ABIE40_RS29525 0.74 -0.1 598,638 + ABIE40_RS29525 0.74 -1.7 598,864 + ABIE40_RS29525 0.82 -0.3 598,865 - ABIE40_RS29525 0.82 -1.2 598,924 - ABIE40_RS29525 0.84 -2.3 599,084 + -1.2 599,084 + -0.3 599,085 - +1.3 599,085 - -0.5 599,201 + +1.3 599,201 + -1.4 599,201 + -2.5 599,202 - +1.3 599,202 - +2.6 599,202 - -1.4 599,202 - +1.1 599,202 - -1.2 599,202 - -2.6 599,522 + -2.5 599,523 - +4.1 599,523 - -3.2 599,523 - -0.6 599,523 - +2.8 599,653 + -0.2 599,673 + -1.1 599,673 + +0.3 599,673 + -0.6 599,673 + +0.7 599,674 - -0.0 599,733 + +0.4 599,733 + -1.7 599,733 + +0.4 599,734 - -1.1 599,841 + -2.7 599,887 - -0.9 599,887 - -2.6 599,887 - +0.3 599,946 + +0.3 599,947 - -1.7 599,947 - -0.7 599,947 - -1.1 599,947 - -2.5 600,011 + -1.0 600,011 + -0.5 600,011 + -1.1 600,011 + -1.5 600,011 + +1.6 600,012 - +1.3 600,012 - -1.7 600,047 + +0.5 600,059 - -1.0 600,084 + -0.6 600,084 + -0.0 600,099 + +2.2 600,099 + -0.3 600,099 + +0.3 600,244 + ABIE40_RS29530 0.16 -4.5 600,245 - ABIE40_RS29530 0.16 +1.0 600,321 + ABIE40_RS29530 0.26 +1.4 600,321 + ABIE40_RS29530 0.26 -2.9 600,322 - ABIE40_RS29530 0.26 -1.2
Or see this region's nucleotide sequence