Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS06790 and ABIE40_RS06795 are separated by 110 nucleotides ABIE40_RS06795 and ABIE40_RS06800 are separated by 81 nucleotides ABIE40_RS06800 and ABIE40_RS06805 are separated by 67 nucleotides ABIE40_RS06805 and ABIE40_RS06810 are separated by 78 nucleotides ABIE40_RS06810 and ABIE40_RS06815 are separated by 360 nucleotides
ABIE40_RS06790: ABIE40_RS06790 - integration host factor subunit alpha, at 1,330,638 to 1,330,976
_RS06790
ABIE40_RS06795: ABIE40_RS06795 - MerR family transcriptional regulator, at 1,331,087 to 1,331,680
_RS06795
ABIE40_RS06800: ABIE40_RS06800 - FAD-dependent oxidoreductase, at 1,331,762 to 1,333,420
_RS06800
ABIE40_RS06805: ABIE40_RS06805 - DUF1236 domain-containing protein, at 1,333,488 to 1,333,805
_RS06805
ABIE40_RS06810: ABIE40_RS06810 - hypothetical protein, at 1,333,884 to 1,334,051
_RS06810
ABIE40_RS06815: ABIE40_RS06815 - cellulose biosynthesis protein BcsN, at 1,334,412 to 1,335,413
_RS06815
Position (kb)
1331
1332
1333
1334 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1330.999 kb on - strand at 1331.005 kb on + strand at 1331.083 kb on + strand at 1331.273 kb on + strand, within ABIE40_RS06795 at 1331.673 kb on + strand at 1331.674 kb on - strand at 1331.721 kb on + strand at 1331.725 kb on + strand at 1331.725 kb on + strand at 1331.725 kb on + strand at 1331.725 kb on + strand at 1331.726 kb on - strand at 1331.726 kb on - strand at 1331.726 kb on - strand at 1331.735 kb on + strand at 1331.767 kb on + strand at 1331.767 kb on + strand at 1331.768 kb on - strand at 1331.768 kb on - strand at 1331.768 kb on - strand at 1331.768 kb on - strand at 1331.769 kb on + strand at 1331.770 kb on - strand at 1331.770 kb on - strand at 1331.770 kb on - strand at 1331.805 kb on + strand at 1331.805 kb on + strand at 1331.805 kb on + strand at 1331.805 kb on + strand at 1331.805 kb on + strand at 1331.805 kb on + strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1331.806 kb on - strand at 1332.107 kb on + strand, within ABIE40_RS06800 at 1332.107 kb on + strand, within ABIE40_RS06800 at 1332.107 kb on + strand, within ABIE40_RS06800 at 1332.107 kb on + strand, within ABIE40_RS06800 at 1332.107 kb on + strand, within ABIE40_RS06800 at 1332.107 kb on + strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.108 kb on - strand, within ABIE40_RS06800 at 1332.237 kb on - strand, within ABIE40_RS06800 at 1332.322 kb on + strand, within ABIE40_RS06800 at 1332.323 kb on - strand, within ABIE40_RS06800 at 1332.323 kb on - strand, within ABIE40_RS06800 at 1332.323 kb on - strand, within ABIE40_RS06800 at 1332.380 kb on + strand, within ABIE40_RS06800 at 1332.485 kb on + strand, within ABIE40_RS06800 at 1332.485 kb on + strand, within ABIE40_RS06800 at 1332.485 kb on + strand, within ABIE40_RS06800 at 1332.647 kb on + strand, within ABIE40_RS06800 at 1332.647 kb on + strand, within ABIE40_RS06800 at 1332.648 kb on - strand, within ABIE40_RS06800 at 1332.710 kb on + strand, within ABIE40_RS06800 at 1332.710 kb on + strand, within ABIE40_RS06800 at 1332.710 kb on + strand, within ABIE40_RS06800 at 1332.711 kb on - strand, within ABIE40_RS06800 at 1332.738 kb on - strand, within ABIE40_RS06800 at 1332.769 kb on + strand, within ABIE40_RS06800 at 1332.769 kb on + strand, within ABIE40_RS06800 at 1332.769 kb on + strand, within ABIE40_RS06800 at 1332.769 kb on + strand, within ABIE40_RS06800 at 1332.769 kb on + strand, within ABIE40_RS06800 at 1332.770 kb on - strand, within ABIE40_RS06800 at 1332.770 kb on - strand, within ABIE40_RS06800 at 1332.770 kb on - strand, within ABIE40_RS06800 at 1332.794 kb on + strand, within ABIE40_RS06800 at 1332.795 kb on - strand, within ABIE40_RS06800 at 1332.858 kb on + strand, within ABIE40_RS06800 at 1332.859 kb on - strand, within ABIE40_RS06800 at 1332.923 kb on + strand, within ABIE40_RS06800 at 1332.923 kb on + strand, within ABIE40_RS06800 at 1332.935 kb on + strand, within ABIE40_RS06800 at 1332.936 kb on - strand, within ABIE40_RS06800 at 1333.017 kb on + strand, within ABIE40_RS06800 at 1333.018 kb on - strand, within ABIE40_RS06800 at 1333.115 kb on + strand, within ABIE40_RS06800 at 1333.115 kb on + strand, within ABIE40_RS06800 at 1333.116 kb on - strand, within ABIE40_RS06800 at 1333.116 kb on - strand, within ABIE40_RS06800 at 1333.116 kb on - strand, within ABIE40_RS06800 at 1333.116 kb on - strand, within ABIE40_RS06800 at 1333.116 kb on - strand, within ABIE40_RS06800 at 1333.262 kb on + strand at 1333.263 kb on - strand at 1333.263 kb on - strand at 1333.556 kb on + strand, within ABIE40_RS06805 at 1333.557 kb on - strand, within ABIE40_RS06805 at 1333.557 kb on - strand, within ABIE40_RS06805 at 1333.557 kb on - strand, within ABIE40_RS06805 at 1333.557 kb on - strand, within ABIE40_RS06805 at 1333.558 kb on + strand, within ABIE40_RS06805 at 1333.558 kb on + strand, within ABIE40_RS06805 at 1333.558 kb on + strand, within ABIE40_RS06805 at 1333.558 kb on + strand, within ABIE40_RS06805 at 1333.558 kb on + strand, within ABIE40_RS06805 at 1333.558 kb on + strand, within ABIE40_RS06805 at 1333.558 kb on + strand, within ABIE40_RS06805 at 1333.559 kb on - strand, within ABIE40_RS06805 at 1333.559 kb on - strand, within ABIE40_RS06805 at 1333.559 kb on - strand, within ABIE40_RS06805 at 1333.559 kb on - strand, within ABIE40_RS06805 at 1333.559 kb on - strand, within ABIE40_RS06805 at 1333.559 kb on - strand, within ABIE40_RS06805 at 1333.559 kb on - strand, within ABIE40_RS06805 at 1333.564 kb on + strand, within ABIE40_RS06805 at 1333.564 kb on + strand, within ABIE40_RS06805 at 1333.564 kb on + strand, within ABIE40_RS06805 at 1333.565 kb on - strand, within ABIE40_RS06805 at 1333.565 kb on - strand, within ABIE40_RS06805 at 1333.565 kb on - strand, within ABIE40_RS06805 at 1333.565 kb on - strand, within ABIE40_RS06805 at 1333.565 kb on - strand, within ABIE40_RS06805 at 1333.575 kb on - strand, within ABIE40_RS06805 at 1333.575 kb on - strand, within ABIE40_RS06805 at 1333.586 kb on + strand, within ABIE40_RS06805 at 1333.639 kb on + strand, within ABIE40_RS06805 at 1333.639 kb on + strand, within ABIE40_RS06805 at 1333.639 kb on + strand, within ABIE40_RS06805 at 1333.639 kb on + strand, within ABIE40_RS06805 at 1333.640 kb on - strand, within ABIE40_RS06805 at 1333.640 kb on - strand, within ABIE40_RS06805 at 1333.640 kb on - strand, within ABIE40_RS06805 at 1333.640 kb on - strand, within ABIE40_RS06805 at 1333.644 kb on + strand, within ABIE40_RS06805 at 1333.645 kb on - strand, within ABIE40_RS06805 at 1333.672 kb on + strand, within ABIE40_RS06805 at 1333.672 kb on + strand, within ABIE40_RS06805 at 1333.672 kb on + strand, within ABIE40_RS06805 at 1333.673 kb on - strand, within ABIE40_RS06805 at 1333.673 kb on - strand, within ABIE40_RS06805 at 1333.673 kb on - strand, within ABIE40_RS06805 at 1333.673 kb on - strand, within ABIE40_RS06805 at 1333.673 kb on - strand, within ABIE40_RS06805 at 1333.673 kb on - strand, within ABIE40_RS06805 at 1333.673 kb on - strand, within ABIE40_RS06805 at 1333.673 kb on - strand, within ABIE40_RS06805 at 1333.925 kb on + strand, within ABIE40_RS06810 at 1333.926 kb on - strand, within ABIE40_RS06810 at 1333.926 kb on - strand, within ABIE40_RS06810 at 1333.926 kb on - strand, within ABIE40_RS06810 at 1333.930 kb on + strand, within ABIE40_RS06810 at 1333.930 kb on + strand, within ABIE40_RS06810 at 1333.931 kb on - strand, within ABIE40_RS06810 at 1333.952 kb on - strand, within ABIE40_RS06810 at 1333.952 kb on - strand, within ABIE40_RS06810 at 1334.080 kb on + strand at 1334.133 kb on + strand at 1334.227 kb on + strand at 1334.227 kb on + strand at 1334.288 kb on + strand at 1334.289 kb on - strand at 1334.302 kb on + strand at 1334.302 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 1,330,999 - +0.1 1,331,005 + +0.4 1,331,083 + -1.0 1,331,273 + ABIE40_RS06795 0.31 -0.8 1,331,673 + +0.8 1,331,674 - +1.2 1,331,721 + -0.2 1,331,725 + +2.7 1,331,725 + -0.7 1,331,725 + -0.8 1,331,725 + -1.5 1,331,726 - -3.4 1,331,726 - +1.2 1,331,726 - +2.2 1,331,735 + +1.2 1,331,767 + -0.1 1,331,767 + +0.5 1,331,768 - -0.4 1,331,768 - -2.3 1,331,768 - -0.6 1,331,768 - +2.6 1,331,769 + +0.0 1,331,770 - -0.2 1,331,770 - +0.4 1,331,770 - -3.2 1,331,805 + +0.9 1,331,805 + +1.0 1,331,805 + +0.6 1,331,805 + +0.4 1,331,805 + -2.6 1,331,805 + -0.5 1,331,806 - -1.9 1,331,806 - -1.1 1,331,806 - +0.9 1,331,806 - +1.9 1,331,806 - +1.4 1,331,806 - +0.1 1,331,806 - -0.6 1,331,806 - -1.5 1,331,806 - -2.8 1,331,806 - +1.7 1,331,806 - +0.5 1,331,806 - -0.6 1,331,806 - +0.2 1,332,107 + ABIE40_RS06800 0.21 +1.8 1,332,107 + ABIE40_RS06800 0.21 +0.3 1,332,107 + ABIE40_RS06800 0.21 -2.8 1,332,107 + ABIE40_RS06800 0.21 +0.7 1,332,107 + ABIE40_RS06800 0.21 -2.7 1,332,107 + ABIE40_RS06800 0.21 -1.8 1,332,108 - ABIE40_RS06800 0.21 +0.5 1,332,108 - ABIE40_RS06800 0.21 -0.1 1,332,108 - ABIE40_RS06800 0.21 -1.7 1,332,108 - ABIE40_RS06800 0.21 -2.3 1,332,108 - ABIE40_RS06800 0.21 -0.8 1,332,108 - ABIE40_RS06800 0.21 +0.6 1,332,108 - ABIE40_RS06800 0.21 +0.3 1,332,108 - ABIE40_RS06800 0.21 -0.2 1,332,108 - ABIE40_RS06800 0.21 -0.7 1,332,108 - ABIE40_RS06800 0.21 +0.4 1,332,108 - ABIE40_RS06800 0.21 +0.6 1,332,108 - ABIE40_RS06800 0.21 -1.7 1,332,237 - ABIE40_RS06800 0.29 +1.4 1,332,322 + ABIE40_RS06800 0.34 +0.3 1,332,323 - ABIE40_RS06800 0.34 +0.5 1,332,323 - ABIE40_RS06800 0.34 -0.0 1,332,323 - ABIE40_RS06800 0.34 +0.5 1,332,380 + ABIE40_RS06800 0.37 +0.9 1,332,485 + ABIE40_RS06800 0.44 +0.5 1,332,485 + ABIE40_RS06800 0.44 -2.2 1,332,485 + ABIE40_RS06800 0.44 +1.0 1,332,647 + ABIE40_RS06800 0.53 -0.4 1,332,647 + ABIE40_RS06800 0.53 +0.3 1,332,648 - ABIE40_RS06800 0.53 +1.4 1,332,710 + ABIE40_RS06800 0.57 -1.2 1,332,710 + ABIE40_RS06800 0.57 +0.2 1,332,710 + ABIE40_RS06800 0.57 -3.1 1,332,711 - ABIE40_RS06800 0.57 -2.8 1,332,738 - ABIE40_RS06800 0.59 -1.3 1,332,769 + ABIE40_RS06800 0.61 +0.7 1,332,769 + ABIE40_RS06800 0.61 +0.8 1,332,769 + ABIE40_RS06800 0.61 -0.1 1,332,769 + ABIE40_RS06800 0.61 -2.9 1,332,769 + ABIE40_RS06800 0.61 -1.0 1,332,770 - ABIE40_RS06800 0.61 +1.4 1,332,770 - ABIE40_RS06800 0.61 +2.4 1,332,770 - ABIE40_RS06800 0.61 -1.9 1,332,794 + ABIE40_RS06800 0.62 -0.6 1,332,795 - ABIE40_RS06800 0.62 +1.0 1,332,858 + ABIE40_RS06800 0.66 +0.7 1,332,859 - ABIE40_RS06800 0.66 +1.6 1,332,923 + ABIE40_RS06800 0.70 +0.6 1,332,923 + ABIE40_RS06800 0.70 +0.2 1,332,935 + ABIE40_RS06800 0.71 +0.4 1,332,936 - ABIE40_RS06800 0.71 -0.4 1,333,017 + ABIE40_RS06800 0.76 +1.1 1,333,018 - ABIE40_RS06800 0.76 -0.4 1,333,115 + ABIE40_RS06800 0.82 +0.2 1,333,115 + ABIE40_RS06800 0.82 +0.7 1,333,116 - ABIE40_RS06800 0.82 +0.7 1,333,116 - ABIE40_RS06800 0.82 -3.5 1,333,116 - ABIE40_RS06800 0.82 -0.7 1,333,116 - ABIE40_RS06800 0.82 -0.8 1,333,116 - ABIE40_RS06800 0.82 -0.1 1,333,262 + +0.2 1,333,263 - +0.2 1,333,263 - +0.9 1,333,556 + ABIE40_RS06805 0.21 -0.9 1,333,557 - ABIE40_RS06805 0.22 -0.5 1,333,557 - ABIE40_RS06805 0.22 +0.5 1,333,557 - ABIE40_RS06805 0.22 -0.9 1,333,557 - ABIE40_RS06805 0.22 +0.7 1,333,558 + ABIE40_RS06805 0.22 -0.3 1,333,558 + ABIE40_RS06805 0.22 -0.5 1,333,558 + ABIE40_RS06805 0.22 -1.3 1,333,558 + ABIE40_RS06805 0.22 -1.5 1,333,558 + ABIE40_RS06805 0.22 +0.8 1,333,558 + ABIE40_RS06805 0.22 -2.5 1,333,558 + ABIE40_RS06805 0.22 +0.8 1,333,559 - ABIE40_RS06805 0.22 +0.7 1,333,559 - ABIE40_RS06805 0.22 -0.9 1,333,559 - ABIE40_RS06805 0.22 +0.6 1,333,559 - ABIE40_RS06805 0.22 +2.0 1,333,559 - ABIE40_RS06805 0.22 +0.8 1,333,559 - ABIE40_RS06805 0.22 +1.3 1,333,559 - ABIE40_RS06805 0.22 +0.6 1,333,564 + ABIE40_RS06805 0.24 +0.7 1,333,564 + ABIE40_RS06805 0.24 -3.3 1,333,564 + ABIE40_RS06805 0.24 -3.4 1,333,565 - ABIE40_RS06805 0.24 +1.0 1,333,565 - ABIE40_RS06805 0.24 +1.4 1,333,565 - ABIE40_RS06805 0.24 +1.1 1,333,565 - ABIE40_RS06805 0.24 +0.4 1,333,565 - ABIE40_RS06805 0.24 +0.3 1,333,575 - ABIE40_RS06805 0.27 +0.2 1,333,575 - ABIE40_RS06805 0.27 +1.2 1,333,586 + ABIE40_RS06805 0.31 +2.3 1,333,639 + ABIE40_RS06805 0.47 +2.8 1,333,639 + ABIE40_RS06805 0.47 +1.3 1,333,639 + ABIE40_RS06805 0.47 -3.3 1,333,639 + ABIE40_RS06805 0.47 +0.8 1,333,640 - ABIE40_RS06805 0.48 +0.2 1,333,640 - ABIE40_RS06805 0.48 +4.0 1,333,640 - ABIE40_RS06805 0.48 -0.5 1,333,640 - ABIE40_RS06805 0.48 -0.6 1,333,644 + ABIE40_RS06805 0.49 -0.1 1,333,645 - ABIE40_RS06805 0.49 +0.8 1,333,672 + ABIE40_RS06805 0.58 -0.6 1,333,672 + ABIE40_RS06805 0.58 +2.6 1,333,672 + ABIE40_RS06805 0.58 -0.3 1,333,673 - ABIE40_RS06805 0.58 -0.9 1,333,673 - ABIE40_RS06805 0.58 +1.3 1,333,673 - ABIE40_RS06805 0.58 -0.6 1,333,673 - ABIE40_RS06805 0.58 -0.4 1,333,673 - ABIE40_RS06805 0.58 +1.3 1,333,673 - ABIE40_RS06805 0.58 +0.4 1,333,673 - ABIE40_RS06805 0.58 +0.4 1,333,673 - ABIE40_RS06805 0.58 -0.6 1,333,925 + ABIE40_RS06810 0.24 -3.4 1,333,926 - ABIE40_RS06810 0.25 +1.6 1,333,926 - ABIE40_RS06810 0.25 -0.3 1,333,926 - ABIE40_RS06810 0.25 +2.3 1,333,930 + ABIE40_RS06810 0.27 -1.1 1,333,930 + ABIE40_RS06810 0.27 +0.0 1,333,931 - ABIE40_RS06810 0.28 -1.5 1,333,952 - ABIE40_RS06810 0.40 -0.4 1,333,952 - ABIE40_RS06810 0.40 +1.5 1,334,080 + +1.8 1,334,133 + -2.2 1,334,227 + +0.5 1,334,227 + +2.4 1,334,288 + +0.0 1,334,289 - +1.8 1,334,302 + +0.3 1,334,302 + -0.5
Or see this region's nucleotide sequence