Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS04595 and ABIE40_RS04600 overlap by 4 nucleotides ABIE40_RS04600 and ABIE40_RS04605 are separated by 244 nucleotides ABIE40_RS04605 and ABIE40_RS04610 are separated by 156 nucleotides ABIE40_RS04610 and ABIE40_RS04615 are separated by 0 nucleotides ABIE40_RS04615 and ABIE40_RS04620 are separated by 98 nucleotides
ABIE40_RS04595: ABIE40_RS04595 - glutathione S-transferase N-terminal domain-containing protein, at 903,607 to 904,227
_RS04595
ABIE40_RS04600: ABIE40_RS04600 - MmcQ/YjbR family DNA-binding protein, at 904,224 to 904,577
_RS04600
ABIE40_RS04605: ABIE40_RS04605 - hypothetical protein, at 904,822 to 905,001
_RS04605
ABIE40_RS04610: ABIE40_RS04610 - LysR substrate-binding domain-containing protein, at 905,158 to 906,054
_RS04610
ABIE40_RS04615: ABIE40_RS04615 - glutathione S-transferase family protein, at 906,055 to 906,714
_RS04615
ABIE40_RS04620: ABIE40_RS04620 - TIGR03808 family TAT-translocated repetitive protein, at 906,813 to 908,138
_RS04620
Position (kb)
905
906
907 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 904.168 kb on + strand at 904.168 kb on + strand at 904.168 kb on + strand at 904.168 kb on + strand at 904.169 kb on - strand at 904.169 kb on - strand at 904.169 kb on - strand at 904.225 kb on + strand at 904.225 kb on + strand at 904.226 kb on - strand at 904.348 kb on + strand, within ABIE40_RS04600 at 904.348 kb on + strand, within ABIE40_RS04600 at 904.348 kb on + strand, within ABIE40_RS04600 at 904.348 kb on + strand, within ABIE40_RS04600 at 904.349 kb on - strand, within ABIE40_RS04600 at 904.349 kb on - strand, within ABIE40_RS04600 at 904.366 kb on + strand, within ABIE40_RS04600 at 904.366 kb on + strand, within ABIE40_RS04600 at 904.366 kb on + strand, within ABIE40_RS04600 at 904.367 kb on - strand, within ABIE40_RS04600 at 904.367 kb on - strand, within ABIE40_RS04600 at 904.367 kb on - strand, within ABIE40_RS04600 at 904.367 kb on - strand, within ABIE40_RS04600 at 904.489 kb on + strand, within ABIE40_RS04600 at 904.633 kb on + strand at 904.633 kb on + strand at 904.633 kb on + strand at 904.634 kb on - strand at 904.634 kb on - strand at 904.634 kb on - strand at 904.662 kb on + strand at 904.662 kb on + strand at 904.662 kb on + strand at 904.781 kb on - strand at 905.004 kb on + strand at 905.005 kb on - strand at 905.005 kb on - strand at 905.005 kb on - strand at 905.062 kb on + strand at 905.062 kb on + strand at 905.062 kb on + strand at 905.062 kb on + strand at 905.063 kb on - strand at 905.063 kb on - strand at 905.063 kb on - strand at 905.063 kb on - strand at 905.084 kb on + strand at 905.085 kb on - strand at 905.085 kb on - strand at 905.085 kb on - strand at 905.146 kb on + strand at 905.157 kb on + strand at 905.158 kb on - strand at 905.256 kb on - strand, within ABIE40_RS04610 at 905.489 kb on - strand, within ABIE40_RS04610 at 905.954 kb on - strand, within ABIE40_RS04610 at 906.136 kb on - strand, within ABIE40_RS04615 at 906.344 kb on + strand, within ABIE40_RS04615 at 906.344 kb on + strand, within ABIE40_RS04615 at 906.344 kb on + strand, within ABIE40_RS04615 at 906.470 kb on + strand, within ABIE40_RS04615 at 906.471 kb on - strand, within ABIE40_RS04615 at 906.471 kb on - strand, within ABIE40_RS04615 at 906.471 kb on - strand, within ABIE40_RS04615 at 906.570 kb on + strand, within ABIE40_RS04615 at 906.570 kb on + strand, within ABIE40_RS04615 at 906.632 kb on + strand, within ABIE40_RS04615 at 906.632 kb on + strand, within ABIE40_RS04615 at 906.633 kb on - strand, within ABIE40_RS04615 at 906.633 kb on - strand, within ABIE40_RS04615 at 906.633 kb on - strand, within ABIE40_RS04615 at 906.668 kb on + strand at 906.701 kb on + strand at 906.701 kb on + strand at 906.702 kb on - strand at 906.702 kb on - strand at 906.746 kb on + strand at 906.746 kb on + strand at 906.746 kb on + strand at 906.747 kb on - strand at 906.747 kb on - strand at 906.749 kb on - strand at 906.749 kb on - strand at 906.806 kb on + strand at 906.806 kb on + strand at 906.806 kb on + strand at 906.806 kb on + strand at 906.806 kb on + strand at 906.806 kb on + strand at 906.806 kb on + strand at 906.806 kb on + strand at 906.807 kb on - strand at 906.807 kb on - strand at 906.807 kb on - strand at 906.807 kb on - strand at 906.807 kb on - strand at 906.904 kb on + strand at 906.904 kb on + strand at 907.046 kb on - strand, within ABIE40_RS04620 at 907.046 kb on - strand, within ABIE40_RS04620 at 907.046 kb on - strand, within ABIE40_RS04620
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 904,168 + +0.3 904,168 + +0.5 904,168 + -1.4 904,168 + -2.4 904,169 - +0.1 904,169 - +0.2 904,169 - -1.8 904,225 + -0.4 904,225 + -0.3 904,226 - -0.2 904,348 + ABIE40_RS04600 0.35 +0.5 904,348 + ABIE40_RS04600 0.35 -0.5 904,348 + ABIE40_RS04600 0.35 -2.4 904,348 + ABIE40_RS04600 0.35 -1.3 904,349 - ABIE40_RS04600 0.35 -0.0 904,349 - ABIE40_RS04600 0.35 +0.7 904,366 + ABIE40_RS04600 0.40 +1.1 904,366 + ABIE40_RS04600 0.40 -0.7 904,366 + ABIE40_RS04600 0.40 +1.3 904,367 - ABIE40_RS04600 0.40 -0.8 904,367 - ABIE40_RS04600 0.40 +0.6 904,367 - ABIE40_RS04600 0.40 +0.3 904,367 - ABIE40_RS04600 0.40 -0.7 904,489 + ABIE40_RS04600 0.75 -0.1 904,633 + +0.4 904,633 + +0.2 904,633 + +0.3 904,634 - +0.7 904,634 - -2.0 904,634 - -2.9 904,662 + -0.3 904,662 + +1.7 904,662 + -0.1 904,781 - +0.7 905,004 + +0.1 905,005 - +0.4 905,005 - -2.4 905,005 - +1.3 905,062 + -1.5 905,062 + -2.9 905,062 + +0.1 905,062 + +0.6 905,063 - +0.6 905,063 - +1.3 905,063 - +0.4 905,063 - -0.3 905,084 + +1.7 905,085 - -1.5 905,085 - -0.2 905,085 - +0.4 905,146 + +0.8 905,157 + -2.9 905,158 - -0.3 905,256 - ABIE40_RS04610 0.11 +0.5 905,489 - ABIE40_RS04610 0.37 -2.4 905,954 - ABIE40_RS04610 0.89 -0.3 906,136 - ABIE40_RS04615 0.12 +0.7 906,344 + ABIE40_RS04615 0.44 +0.0 906,344 + ABIE40_RS04615 0.44 -0.5 906,344 + ABIE40_RS04615 0.44 -3.1 906,470 + ABIE40_RS04615 0.63 +0.9 906,471 - ABIE40_RS04615 0.63 +1.4 906,471 - ABIE40_RS04615 0.63 +0.3 906,471 - ABIE40_RS04615 0.63 +0.3 906,570 + ABIE40_RS04615 0.78 -0.2 906,570 + ABIE40_RS04615 0.78 +1.5 906,632 + ABIE40_RS04615 0.87 -0.3 906,632 + ABIE40_RS04615 0.87 -2.9 906,633 - ABIE40_RS04615 0.88 +0.1 906,633 - ABIE40_RS04615 0.88 -0.6 906,633 - ABIE40_RS04615 0.88 +0.6 906,668 + +1.5 906,701 + -0.3 906,701 + -0.9 906,702 - -1.0 906,702 - +2.1 906,746 + -0.2 906,746 + +1.7 906,746 + -0.5 906,747 - +0.5 906,747 - +0.4 906,749 - -1.5 906,749 - +0.3 906,806 + +1.4 906,806 + +1.0 906,806 + -1.4 906,806 + -1.9 906,806 + -2.4 906,806 + -3.2 906,806 + +1.1 906,806 + -0.2 906,807 - +0.4 906,807 - +1.4 906,807 - +2.0 906,807 - -1.6 906,807 - +0.1 906,904 + -2.0 906,904 + -3.9 907,046 - ABIE40_RS04620 0.18 -0.3 907,046 - ABIE40_RS04620 0.18 -0.3 907,046 - ABIE40_RS04620 0.18 -1.5
Or see this region's nucleotide sequence