Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS04160 and ABIE40_RS04165 are separated by 5 nucleotides ABIE40_RS04165 and ABIE40_RS04170 are separated by 29 nucleotides ABIE40_RS04170 and ABIE40_RS04175 are separated by 67 nucleotides ABIE40_RS04175 and ABIE40_RS04180 overlap by 78 nucleotides ABIE40_RS04180 and ABIE40_RS04185 are separated by 101 nucleotides ABIE40_RS04185 and ABIE40_RS04190 are separated by 87 nucleotides
ABIE40_RS04160: ABIE40_RS04160 - type II toxin-antitoxin system ParD family antitoxin, at 823,701 to 823,940
_RS04160
ABIE40_RS04165: ABIE40_RS04165 - type II toxin-antitoxin system RelE/ParE family toxin, at 823,946 to 824,248
_RS04165
ABIE40_RS04170: ABIE40_RS04170 - peroxiredoxin, at 824,278 to 824,763
_RS04170
ABIE40_RS04175: ABIE40_RS04175 - protein-disulfide reductase DsbD domain-containing protein, at 824,831 to 825,581
_RS04175
ABIE40_RS04180: ABIE40_RS04180 - hypothetical protein, at 825,504 to 825,716
_RS04180
ABIE40_RS04185: ABIE40_RS04185 - YqgE/AlgH family protein, at 825,818 to 826,423
_RS04185
ABIE40_RS04190: ABIE40_RS04190 - CsbD family protein, at 826,511 to 826,702
_RS04190
Position (kb) 824
825
826 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 823.880 kb on - strand, within ABIE40_RS04160 at 823.895 kb on - strand, within ABIE40_RS04160 at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.938 kb on + strand at 823.939 kb on - strand at 823.939 kb on - strand at 823.939 kb on - strand at 823.939 kb on - strand at 823.939 kb on - strand at 823.939 kb on - strand at 823.939 kb on - strand at 823.939 kb on - strand at 823.943 kb on + strand at 823.943 kb on + strand at 823.944 kb on - strand at 823.962 kb on - strand at 823.986 kb on + strand, within ABIE40_RS04165 at 823.986 kb on + strand, within ABIE40_RS04165 at 823.986 kb on + strand, within ABIE40_RS04165 at 823.986 kb on + strand, within ABIE40_RS04165 at 823.987 kb on - strand, within ABIE40_RS04165 at 823.987 kb on - strand, within ABIE40_RS04165 at 824.003 kb on + strand, within ABIE40_RS04165 at 824.003 kb on + strand, within ABIE40_RS04165 at 824.004 kb on - strand, within ABIE40_RS04165 at 824.043 kb on + strand, within ABIE40_RS04165 at 824.043 kb on + strand, within ABIE40_RS04165 at 824.044 kb on - strand, within ABIE40_RS04165 at 824.068 kb on + strand, within ABIE40_RS04165 at 824.068 kb on + strand, within ABIE40_RS04165 at 824.069 kb on - strand, within ABIE40_RS04165 at 824.069 kb on - strand, within ABIE40_RS04165 at 824.069 kb on - strand, within ABIE40_RS04165 at 824.069 kb on - strand, within ABIE40_RS04165 at 824.069 kb on - strand, within ABIE40_RS04165 at 824.069 kb on - strand, within ABIE40_RS04165 at 824.128 kb on + strand, within ABIE40_RS04165 at 824.128 kb on + strand, within ABIE40_RS04165 at 824.128 kb on + strand, within ABIE40_RS04165 at 824.140 kb on + strand, within ABIE40_RS04165 at 824.141 kb on - strand, within ABIE40_RS04165 at 824.141 kb on - strand, within ABIE40_RS04165 at 824.153 kb on + strand, within ABIE40_RS04165 at 824.153 kb on + strand, within ABIE40_RS04165 at 824.153 kb on + strand, within ABIE40_RS04165 at 824.153 kb on + strand, within ABIE40_RS04165 at 824.153 kb on + strand, within ABIE40_RS04165 at 824.153 kb on + strand, within ABIE40_RS04165 at 824.153 kb on + strand, within ABIE40_RS04165 at 824.153 kb on + strand, within ABIE40_RS04165 at 824.153 kb on + strand, within ABIE40_RS04165 at 824.153 kb on + strand, within ABIE40_RS04165 at 824.154 kb on - strand, within ABIE40_RS04165 at 824.154 kb on - strand, within ABIE40_RS04165 at 824.154 kb on - strand, within ABIE40_RS04165 at 824.154 kb on - strand, within ABIE40_RS04165 at 824.154 kb on - strand, within ABIE40_RS04165 at 824.154 kb on - strand, within ABIE40_RS04165 at 824.238 kb on + strand at 824.238 kb on + strand at 824.238 kb on + strand at 824.239 kb on - strand at 824.239 kb on - strand at 824.239 kb on - strand at 824.382 kb on - strand, within ABIE40_RS04170 at 824.595 kb on - strand, within ABIE40_RS04170 at 824.819 kb on + strand at 824.819 kb on + strand at 824.819 kb on + strand at 824.819 kb on + strand at 824.819 kb on + strand at 824.819 kb on + strand at 824.819 kb on + strand at 824.820 kb on - strand at 824.820 kb on - strand at 824.820 kb on - strand at 824.821 kb on + strand at 824.821 kb on + strand at 824.821 kb on + strand at 824.821 kb on + strand at 824.821 kb on + strand at 824.821 kb on + strand at 824.821 kb on + strand at 824.821 kb on + strand at 824.822 kb on - strand at 824.822 kb on - strand at 824.822 kb on - strand at 824.822 kb on - strand at 824.822 kb on - strand at 824.822 kb on - strand at 824.910 kb on + strand, within ABIE40_RS04175 at 824.910 kb on + strand, within ABIE40_RS04175 at 824.911 kb on - strand, within ABIE40_RS04175 at 824.911 kb on - strand, within ABIE40_RS04175 at 824.911 kb on - strand, within ABIE40_RS04175 at 824.911 kb on - strand, within ABIE40_RS04175 at 824.911 kb on - strand, within ABIE40_RS04175 at 824.994 kb on + strand, within ABIE40_RS04175 at 824.994 kb on + strand, within ABIE40_RS04175 at 824.995 kb on - strand, within ABIE40_RS04175 at 824.995 kb on - strand, within ABIE40_RS04175 at 824.995 kb on - strand, within ABIE40_RS04175 at 824.995 kb on - strand, within ABIE40_RS04175 at 824.996 kb on - strand, within ABIE40_RS04175 at 825.314 kb on + strand, within ABIE40_RS04175 at 825.314 kb on + strand, within ABIE40_RS04175 at 825.314 kb on + strand, within ABIE40_RS04175 at 825.314 kb on + strand, within ABIE40_RS04175 at 825.331 kb on - strand, within ABIE40_RS04175 at 825.361 kb on + strand, within ABIE40_RS04175 at 825.361 kb on + strand, within ABIE40_RS04175 at 825.361 kb on + strand, within ABIE40_RS04175 at 825.361 kb on + strand, within ABIE40_RS04175 at 825.361 kb on + strand, within ABIE40_RS04175 at 825.362 kb on - strand, within ABIE40_RS04175 at 825.362 kb on - strand, within ABIE40_RS04175 at 825.436 kb on + strand, within ABIE40_RS04175 at 825.462 kb on + strand, within ABIE40_RS04175 at 825.462 kb on + strand, within ABIE40_RS04175 at 825.462 kb on + strand, within ABIE40_RS04175 at 825.462 kb on + strand, within ABIE40_RS04175 at 825.463 kb on - strand, within ABIE40_RS04175 at 825.463 kb on - strand, within ABIE40_RS04175 at 825.665 kb on + strand, within ABIE40_RS04180 at 825.666 kb on - strand, within ABIE40_RS04180 at 825.666 kb on - strand, within ABIE40_RS04180 at 825.713 kb on - strand at 825.727 kb on + strand at 825.728 kb on - strand at 825.817 kb on + strand at 825.817 kb on + strand at 825.817 kb on + strand at 825.817 kb on + strand at 825.817 kb on + strand at 825.817 kb on + strand at 825.817 kb on + strand at 825.818 kb on - strand at 825.818 kb on - strand at 825.818 kb on - strand at 825.926 kb on + strand, within ABIE40_RS04185 at 825.926 kb on + strand, within ABIE40_RS04185 at 825.926 kb on + strand, within ABIE40_RS04185 at 825.927 kb on - strand, within ABIE40_RS04185 at 825.928 kb on + strand, within ABIE40_RS04185 at 825.928 kb on + strand, within ABIE40_RS04185 at 825.928 kb on + strand, within ABIE40_RS04185 at 825.928 kb on + strand, within ABIE40_RS04185 at 825.928 kb on + strand, within ABIE40_RS04185 at 825.928 kb on + strand, within ABIE40_RS04185 at 825.928 kb on + strand, within ABIE40_RS04185 at 826.130 kb on + strand, within ABIE40_RS04185 at 826.130 kb on + strand, within ABIE40_RS04185 at 826.165 kb on + strand, within ABIE40_RS04185 at 826.166 kb on - strand, within ABIE40_RS04185 at 826.248 kb on - strand, within ABIE40_RS04185 at 826.355 kb on + strand, within ABIE40_RS04185 at 826.356 kb on - strand, within ABIE40_RS04185 at 826.446 kb on + strand at 826.446 kb on + strand at 826.446 kb on + strand at 826.447 kb on - strand at 826.456 kb on + strand at 826.457 kb on - strand at 826.457 kb on - strand at 826.510 kb on + strand at 826.517 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 823,880 - ABIE40_RS04160 0.75 +0.5 823,895 - ABIE40_RS04160 0.81 -3.6 823,938 + +1.2 823,938 + -0.5 823,938 + +1.7 823,938 + -3.1 823,938 + -0.0 823,938 + +0.0 823,938 + +1.4 823,938 + +0.3 823,938 + +0.3 823,938 + +1.1 823,938 + -0.9 823,938 + -2.6 823,938 + +0.3 823,938 + +2.1 823,938 + -0.0 823,939 - -2.6 823,939 - +0.1 823,939 - -0.9 823,939 - +0.5 823,939 - +0.0 823,939 - +1.2 823,939 - -0.2 823,939 - +1.1 823,943 + -0.4 823,943 + -2.0 823,944 - -3.0 823,962 - -0.5 823,986 + ABIE40_RS04165 0.13 +1.5 823,986 + ABIE40_RS04165 0.13 -0.1 823,986 + ABIE40_RS04165 0.13 +0.2 823,986 + ABIE40_RS04165 0.13 -1.4 823,987 - ABIE40_RS04165 0.14 -0.7 823,987 - ABIE40_RS04165 0.14 -0.9 824,003 + ABIE40_RS04165 0.19 -0.3 824,003 + ABIE40_RS04165 0.19 -0.6 824,004 - ABIE40_RS04165 0.19 +1.3 824,043 + ABIE40_RS04165 0.32 -3.0 824,043 + ABIE40_RS04165 0.32 +0.5 824,044 - ABIE40_RS04165 0.32 +0.1 824,068 + ABIE40_RS04165 0.40 -3.5 824,068 + ABIE40_RS04165 0.40 -0.0 824,069 - ABIE40_RS04165 0.41 -0.3 824,069 - ABIE40_RS04165 0.41 -3.1 824,069 - ABIE40_RS04165 0.41 -0.3 824,069 - ABIE40_RS04165 0.41 -3.1 824,069 - ABIE40_RS04165 0.41 +1.1 824,069 - ABIE40_RS04165 0.41 -0.2 824,128 + ABIE40_RS04165 0.60 -1.9 824,128 + ABIE40_RS04165 0.60 +0.4 824,128 + ABIE40_RS04165 0.60 -2.4 824,140 + ABIE40_RS04165 0.64 +1.5 824,141 - ABIE40_RS04165 0.64 +0.1 824,141 - ABIE40_RS04165 0.64 -1.9 824,153 + ABIE40_RS04165 0.68 +1.2 824,153 + ABIE40_RS04165 0.68 +0.1 824,153 + ABIE40_RS04165 0.68 -2.1 824,153 + ABIE40_RS04165 0.68 -0.9 824,153 + ABIE40_RS04165 0.68 -1.0 824,153 + ABIE40_RS04165 0.68 -0.8 824,153 + ABIE40_RS04165 0.68 +0.5 824,153 + ABIE40_RS04165 0.68 -3.4 824,153 + ABIE40_RS04165 0.68 -0.4 824,153 + ABIE40_RS04165 0.68 +0.9 824,154 - ABIE40_RS04165 0.69 -1.6 824,154 - ABIE40_RS04165 0.69 -1.6 824,154 - ABIE40_RS04165 0.69 +0.5 824,154 - ABIE40_RS04165 0.69 -0.0 824,154 - ABIE40_RS04165 0.69 -1.6 824,154 - ABIE40_RS04165 0.69 -0.3 824,238 + +0.3 824,238 + +0.8 824,238 + +0.5 824,239 - -0.6 824,239 - -0.0 824,239 - +0.4 824,382 - ABIE40_RS04170 0.21 +0.4 824,595 - ABIE40_RS04170 0.65 +0.7 824,819 + -0.5 824,819 + +1.2 824,819 + +0.1 824,819 + -0.5 824,819 + +1.9 824,819 + -3.3 824,819 + -1.7 824,820 - -3.1 824,820 - +0.7 824,820 - +0.7 824,821 + -2.0 824,821 + +2.1 824,821 + -0.7 824,821 + -2.6 824,821 + -0.7 824,821 + +0.8 824,821 + +0.3 824,821 + +1.1 824,822 - -1.3 824,822 - +1.2 824,822 - +0.4 824,822 - -0.1 824,822 - +1.8 824,822 - +0.7 824,910 + ABIE40_RS04175 0.11 +0.4 824,910 + ABIE40_RS04175 0.11 +1.1 824,911 - ABIE40_RS04175 0.11 +0.9 824,911 - ABIE40_RS04175 0.11 +0.0 824,911 - ABIE40_RS04175 0.11 +2.6 824,911 - ABIE40_RS04175 0.11 +1.9 824,911 - ABIE40_RS04175 0.11 -0.9 824,994 + ABIE40_RS04175 0.22 +1.3 824,994 + ABIE40_RS04175 0.22 -1.3 824,995 - ABIE40_RS04175 0.22 +0.5 824,995 - ABIE40_RS04175 0.22 +0.6 824,995 - ABIE40_RS04175 0.22 +2.6 824,995 - ABIE40_RS04175 0.22 +2.0 824,996 - ABIE40_RS04175 0.22 -2.7 825,314 + ABIE40_RS04175 0.64 -0.2 825,314 + ABIE40_RS04175 0.64 -0.3 825,314 + ABIE40_RS04175 0.64 -0.4 825,314 + ABIE40_RS04175 0.64 -0.1 825,331 - ABIE40_RS04175 0.67 -0.4 825,361 + ABIE40_RS04175 0.71 +0.7 825,361 + ABIE40_RS04175 0.71 +1.0 825,361 + ABIE40_RS04175 0.71 +1.0 825,361 + ABIE40_RS04175 0.71 -1.7 825,361 + ABIE40_RS04175 0.71 +1.7 825,362 - ABIE40_RS04175 0.71 -0.3 825,362 - ABIE40_RS04175 0.71 -0.5 825,436 + ABIE40_RS04175 0.81 +2.4 825,462 + ABIE40_RS04175 0.84 +0.6 825,462 + ABIE40_RS04175 0.84 -0.1 825,462 + ABIE40_RS04175 0.84 -1.7 825,462 + ABIE40_RS04175 0.84 +1.6 825,463 - ABIE40_RS04175 0.84 +2.1 825,463 - ABIE40_RS04175 0.84 -0.2 825,665 + ABIE40_RS04180 0.76 -2.6 825,666 - ABIE40_RS04180 0.76 -0.8 825,666 - ABIE40_RS04180 0.76 +0.9 825,713 - +0.2 825,727 + -0.8 825,728 - +3.1 825,817 + -0.6 825,817 + -1.8 825,817 + +1.8 825,817 + -0.7 825,817 + -2.6 825,817 + -1.0 825,817 + +0.3 825,818 - +0.5 825,818 - -0.3 825,818 - -2.4 825,926 + ABIE40_RS04185 0.18 -2.7 825,926 + ABIE40_RS04185 0.18 -1.1 825,926 + ABIE40_RS04185 0.18 +3.5 825,927 - ABIE40_RS04185 0.18 -1.4 825,928 + ABIE40_RS04185 0.18 -2.2 825,928 + ABIE40_RS04185 0.18 -1.8 825,928 + ABIE40_RS04185 0.18 -3.5 825,928 + ABIE40_RS04185 0.18 +0.5 825,928 + ABIE40_RS04185 0.18 -3.6 825,928 + ABIE40_RS04185 0.18 +0.5 825,928 + ABIE40_RS04185 0.18 -2.0 826,130 + ABIE40_RS04185 0.51 -0.8 826,130 + ABIE40_RS04185 0.51 -0.8 826,165 + ABIE40_RS04185 0.57 -0.9 826,166 - ABIE40_RS04185 0.57 -0.3 826,248 - ABIE40_RS04185 0.71 -2.0 826,355 + ABIE40_RS04185 0.89 +1.3 826,356 - ABIE40_RS04185 0.89 -0.3 826,446 + +2.7 826,446 + -1.1 826,446 + -1.5 826,447 - -2.5 826,456 + -0.3 826,457 - +0.5 826,457 - -1.1 826,510 + -0.1 826,517 - -1.9
Or see this region's nucleotide sequence