Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS00630 and ABIE40_RS00635 are separated by 8 nucleotides ABIE40_RS00635 and ABIE40_RS00640 are separated by 95 nucleotides ABIE40_RS00640 and ABIE40_RS00645 are separated by 157 nucleotides
ABIE40_RS00630: ABIE40_RS00630 - ABC transporter ATP-binding protein, at 130,027 to 131,664
_RS00630
ABIE40_RS00635: ABIE40_RS00635 - glyoxylate/hydroxypyruvate reductase A, at 131,673 to 132,632
_RS00635
ABIE40_RS00640: ABIE40_RS00640 - hypothetical protein, at 132,728 to 133,093
_RS00640
ABIE40_RS00645: ABIE40_RS00645 - nicotinate phosphoribosyltransferase, at 133,251 to 134,555
_RS00645
Position (kb)
131
132
133 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 130.789 kb on + strand, within ABIE40_RS00630 at 130.789 kb on + strand, within ABIE40_RS00630 at 130.789 kb on + strand, within ABIE40_RS00630 at 130.790 kb on - strand, within ABIE40_RS00630 at 130.790 kb on - strand, within ABIE40_RS00630 at 130.790 kb on - strand, within ABIE40_RS00630 at 130.791 kb on + strand, within ABIE40_RS00630 at 130.792 kb on - strand, within ABIE40_RS00630 at 130.931 kb on + strand, within ABIE40_RS00630 at 131.080 kb on - strand, within ABIE40_RS00630 at 131.081 kb on + strand, within ABIE40_RS00630 at 131.081 kb on + strand, within ABIE40_RS00630 at 131.081 kb on + strand, within ABIE40_RS00630 at 131.082 kb on - strand, within ABIE40_RS00630 at 131.082 kb on - strand, within ABIE40_RS00630 at 131.082 kb on - strand, within ABIE40_RS00630 at 131.082 kb on - strand, within ABIE40_RS00630 at 131.082 kb on - strand, within ABIE40_RS00630 at 131.082 kb on - strand, within ABIE40_RS00630 at 131.082 kb on - strand, within ABIE40_RS00630 at 131.149 kb on + strand, within ABIE40_RS00630 at 131.207 kb on + strand, within ABIE40_RS00630 at 131.207 kb on + strand, within ABIE40_RS00630 at 131.471 kb on - strand, within ABIE40_RS00630 at 131.471 kb on - strand, within ABIE40_RS00630 at 131.596 kb on + strand at 131.596 kb on + strand at 131.597 kb on - strand at 131.662 kb on + strand at 131.663 kb on - strand at 131.663 kb on - strand at 131.663 kb on - strand at 131.663 kb on - strand at 131.663 kb on - strand at 131.817 kb on + strand, within ABIE40_RS00635 at 131.903 kb on + strand, within ABIE40_RS00635 at 131.904 kb on - strand, within ABIE40_RS00635 at 132.252 kb on + strand, within ABIE40_RS00635 at 132.300 kb on + strand, within ABIE40_RS00635 at 132.300 kb on + strand, within ABIE40_RS00635 at 132.300 kb on + strand, within ABIE40_RS00635 at 132.300 kb on + strand, within ABIE40_RS00635 at 132.300 kb on + strand, within ABIE40_RS00635 at 132.301 kb on - strand, within ABIE40_RS00635 at 132.301 kb on - strand, within ABIE40_RS00635 at 132.301 kb on - strand, within ABIE40_RS00635 at 132.400 kb on + strand, within ABIE40_RS00635 at 133.005 kb on + strand, within ABIE40_RS00640 at 133.005 kb on + strand, within ABIE40_RS00640 at 133.108 kb on + strand at 133.169 kb on + strand at 133.170 kb on - strand at 133.170 kb on - strand at 133.281 kb on + strand at 133.282 kb on - strand at 133.339 kb on - strand at 133.339 kb on - strand at 133.339 kb on - strand at 133.339 kb on - strand at 133.533 kb on + strand, within ABIE40_RS00645 at 133.533 kb on + strand, within ABIE40_RS00645 at 133.533 kb on + strand, within ABIE40_RS00645 at 133.533 kb on + strand, within ABIE40_RS00645 at 133.533 kb on + strand, within ABIE40_RS00645 at 133.534 kb on - strand, within ABIE40_RS00645 at 133.534 kb on - strand, within ABIE40_RS00645 at 133.599 kb on + strand, within ABIE40_RS00645 at 133.626 kb on + strand, within ABIE40_RS00645 at 133.626 kb on + strand, within ABIE40_RS00645 at 133.626 kb on + strand, within ABIE40_RS00645 at 133.626 kb on + strand, within ABIE40_RS00645 at 133.627 kb on - strand, within ABIE40_RS00645 at 133.627 kb on - strand, within ABIE40_RS00645 at 133.627 kb on - strand, within ABIE40_RS00645 at 133.627 kb on - strand, within ABIE40_RS00645
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 130,789 + ABIE40_RS00630 0.47 -1.2 130,789 + ABIE40_RS00630 0.47 +0.1 130,789 + ABIE40_RS00630 0.47 -3.2 130,790 - ABIE40_RS00630 0.47 -0.7 130,790 - ABIE40_RS00630 0.47 -1.7 130,790 - ABIE40_RS00630 0.47 +0.8 130,791 + ABIE40_RS00630 0.47 -0.8 130,792 - ABIE40_RS00630 0.47 +2.0 130,931 + ABIE40_RS00630 0.55 -0.9 131,080 - ABIE40_RS00630 0.64 -1.8 131,081 + ABIE40_RS00630 0.64 -1.8 131,081 + ABIE40_RS00630 0.64 +0.4 131,081 + ABIE40_RS00630 0.64 -2.5 131,082 - ABIE40_RS00630 0.64 -2.0 131,082 - ABIE40_RS00630 0.64 -2.8 131,082 - ABIE40_RS00630 0.64 -1.9 131,082 - ABIE40_RS00630 0.64 -2.2 131,082 - ABIE40_RS00630 0.64 -2.3 131,082 - ABIE40_RS00630 0.64 +2.0 131,082 - ABIE40_RS00630 0.64 +0.4 131,149 + ABIE40_RS00630 0.68 -0.4 131,207 + ABIE40_RS00630 0.72 -0.5 131,207 + ABIE40_RS00630 0.72 +0.4 131,471 - ABIE40_RS00630 0.88 -2.7 131,471 - ABIE40_RS00630 0.88 -3.8 131,596 + -1.5 131,596 + -0.4 131,597 - -0.8 131,662 + +1.0 131,663 - -1.6 131,663 - +0.8 131,663 - -1.4 131,663 - -0.1 131,663 - -0.4 131,817 + ABIE40_RS00635 0.15 -0.0 131,903 + ABIE40_RS00635 0.24 +0.6 131,904 - ABIE40_RS00635 0.24 -1.4 132,252 + ABIE40_RS00635 0.60 +0.0 132,300 + ABIE40_RS00635 0.65 +0.5 132,300 + ABIE40_RS00635 0.65 +1.6 132,300 + ABIE40_RS00635 0.65 +4.3 132,300 + ABIE40_RS00635 0.65 +1.8 132,300 + ABIE40_RS00635 0.65 -0.8 132,301 - ABIE40_RS00635 0.65 +0.4 132,301 - ABIE40_RS00635 0.65 +0.7 132,301 - ABIE40_RS00635 0.65 +0.7 132,400 + ABIE40_RS00635 0.76 -0.3 133,005 + ABIE40_RS00640 0.76 +0.5 133,005 + ABIE40_RS00640 0.76 +0.5 133,108 + +1.5 133,169 + -1.5 133,170 - +0.5 133,170 - -1.2 133,281 + -0.3 133,282 - +0.0 133,339 - +0.5 133,339 - +2.1 133,339 - +0.1 133,339 - +0.3 133,533 + ABIE40_RS00645 0.22 -0.6 133,533 + ABIE40_RS00645 0.22 -0.0 133,533 + ABIE40_RS00645 0.22 +0.5 133,533 + ABIE40_RS00645 0.22 -0.6 133,533 + ABIE40_RS00645 0.22 +0.2 133,534 - ABIE40_RS00645 0.22 +0.5 133,534 - ABIE40_RS00645 0.22 -1.2 133,599 + ABIE40_RS00645 0.27 -1.8 133,626 + ABIE40_RS00645 0.29 -1.6 133,626 + ABIE40_RS00645 0.29 +0.5 133,626 + ABIE40_RS00645 0.29 +0.2 133,626 + ABIE40_RS00645 0.29 -1.0 133,627 - ABIE40_RS00645 0.29 -0.1 133,627 - ABIE40_RS00645 0.29 -0.5 133,627 - ABIE40_RS00645 0.29 -2.0 133,627 - ABIE40_RS00645 0.29 -0.0
Or see this region's nucleotide sequence