Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS12440 and ABIE40_RS12445 overlap by 4 nucleotides ABIE40_RS12445 and ABIE40_RS12450 are separated by 15 nucleotides ABIE40_RS12450 and ABIE40_RS12455 are separated by 184 nucleotides
ABIE40_RS12440: ABIE40_RS12440 - dTMP kinase, at 2,431,011 to 2,431,628
_RS12440
ABIE40_RS12445: ABIE40_RS12445 - metallophosphoesterase, at 2,431,625 to 2,432,452
_RS12445
ABIE40_RS12450: ABIE40_RS12450 - hypothetical protein, at 2,432,468 to 2,432,986
_RS12450
ABIE40_RS12455: ABIE40_RS12455 - type IV secretory system conjugative DNA transfer family protein, at 2,433,171 to 2,433,830
_RS12455
Position (kb)
2432
2433 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2431.877 kb on - strand, within ABIE40_RS12445 at 2432.187 kb on - strand, within ABIE40_RS12445 at 2432.187 kb on - strand, within ABIE40_RS12445 at 2432.193 kb on - strand, within ABIE40_RS12445 at 2432.235 kb on - strand, within ABIE40_RS12445 at 2432.509 kb on + strand at 2432.619 kb on + strand, within ABIE40_RS12450 at 2432.619 kb on + strand, within ABIE40_RS12450 at 2432.620 kb on - strand, within ABIE40_RS12450 at 2432.638 kb on + strand, within ABIE40_RS12450 at 2432.638 kb on + strand, within ABIE40_RS12450 at 2432.638 kb on + strand, within ABIE40_RS12450 at 2432.638 kb on + strand, within ABIE40_RS12450 at 2432.638 kb on + strand, within ABIE40_RS12450 at 2432.638 kb on + strand, within ABIE40_RS12450 at 2432.638 kb on + strand, within ABIE40_RS12450 at 2432.638 kb on + strand, within ABIE40_RS12450 at 2432.638 kb on + strand, within ABIE40_RS12450 at 2432.657 kb on + strand, within ABIE40_RS12450 at 2432.681 kb on + strand, within ABIE40_RS12450 at 2432.779 kb on + strand, within ABIE40_RS12450 at 2432.779 kb on + strand, within ABIE40_RS12450 at 2432.779 kb on + strand, within ABIE40_RS12450 at 2432.791 kb on + strand, within ABIE40_RS12450 at 2432.888 kb on - strand, within ABIE40_RS12450 at 2432.888 kb on - strand, within ABIE40_RS12450 at 2433.047 kb on + strand at 2433.048 kb on - strand at 2433.060 kb on + strand at 2433.061 kb on - strand at 2433.093 kb on + strand at 2433.094 kb on - strand at 2433.112 kb on + strand at 2433.112 kb on + strand at 2433.112 kb on + strand at 2433.113 kb on - strand at 2433.145 kb on + strand at 2433.169 kb on + strand at 2433.172 kb on + strand at 2433.172 kb on + strand at 2433.172 kb on + strand at 2433.172 kb on + strand at 2433.172 kb on + strand at 2433.172 kb on + strand at 2433.173 kb on - strand at 2433.173 kb on - strand at 2433.173 kb on - strand at 2433.173 kb on - strand at 2433.173 kb on - strand at 2433.297 kb on + strand, within ABIE40_RS12455 at 2433.297 kb on + strand, within ABIE40_RS12455 at 2433.298 kb on - strand, within ABIE40_RS12455 at 2433.298 kb on - strand, within ABIE40_RS12455 at 2433.300 kb on - strand, within ABIE40_RS12455 at 2433.473 kb on + strand, within ABIE40_RS12455 at 2433.473 kb on + strand, within ABIE40_RS12455 at 2433.474 kb on - strand, within ABIE40_RS12455 at 2433.474 kb on - strand, within ABIE40_RS12455 at 2433.514 kb on + strand, within ABIE40_RS12455 at 2433.514 kb on + strand, within ABIE40_RS12455 at 2433.515 kb on - strand, within ABIE40_RS12455 at 2433.515 kb on - strand, within ABIE40_RS12455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 2,431,877 - ABIE40_RS12445 0.30 -1.5 2,432,187 - ABIE40_RS12445 0.68 -0.2 2,432,187 - ABIE40_RS12445 0.68 +0.1 2,432,193 - ABIE40_RS12445 0.69 -0.2 2,432,235 - ABIE40_RS12445 0.74 -0.5 2,432,509 + +1.1 2,432,619 + ABIE40_RS12450 0.29 -0.4 2,432,619 + ABIE40_RS12450 0.29 -1.3 2,432,620 - ABIE40_RS12450 0.29 +0.0 2,432,638 + ABIE40_RS12450 0.33 -0.8 2,432,638 + ABIE40_RS12450 0.33 -1.6 2,432,638 + ABIE40_RS12450 0.33 -0.7 2,432,638 + ABIE40_RS12450 0.33 +0.6 2,432,638 + ABIE40_RS12450 0.33 +1.4 2,432,638 + ABIE40_RS12450 0.33 -1.3 2,432,638 + ABIE40_RS12450 0.33 -1.6 2,432,638 + ABIE40_RS12450 0.33 -1.5 2,432,638 + ABIE40_RS12450 0.33 +0.8 2,432,657 + ABIE40_RS12450 0.36 +0.4 2,432,681 + ABIE40_RS12450 0.41 -2.6 2,432,779 + ABIE40_RS12450 0.60 -1.6 2,432,779 + ABIE40_RS12450 0.60 -2.2 2,432,779 + ABIE40_RS12450 0.60 +0.4 2,432,791 + ABIE40_RS12450 0.62 -1.1 2,432,888 - ABIE40_RS12450 0.81 -0.8 2,432,888 - ABIE40_RS12450 0.81 -0.4 2,433,047 + -2.2 2,433,048 - -0.2 2,433,060 + +1.0 2,433,061 - -0.4 2,433,093 + -2.7 2,433,094 - -1.4 2,433,112 + -0.6 2,433,112 + -1.5 2,433,112 + -1.0 2,433,113 - -0.8 2,433,145 + -1.1 2,433,169 + +0.8 2,433,172 + -0.2 2,433,172 + +0.8 2,433,172 + +1.8 2,433,172 + -2.3 2,433,172 + -0.4 2,433,172 + -1.7 2,433,173 - +1.8 2,433,173 - -1.4 2,433,173 - -1.3 2,433,173 - -0.3 2,433,173 - -2.0 2,433,297 + ABIE40_RS12455 0.19 -2.1 2,433,297 + ABIE40_RS12455 0.19 +1.4 2,433,298 - ABIE40_RS12455 0.19 +1.6 2,433,298 - ABIE40_RS12455 0.19 -0.0 2,433,300 - ABIE40_RS12455 0.20 -0.7 2,433,473 + ABIE40_RS12455 0.46 -1.2 2,433,473 + ABIE40_RS12455 0.46 -0.0 2,433,474 - ABIE40_RS12455 0.46 +0.4 2,433,474 - ABIE40_RS12455 0.46 -0.2 2,433,514 + ABIE40_RS12455 0.52 -0.5 2,433,514 + ABIE40_RS12455 0.52 -2.1 2,433,515 - ABIE40_RS12455 0.52 +0.4 2,433,515 - ABIE40_RS12455 0.52 +1.0
Or see this region's nucleotide sequence