Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS14885 and ABIE40_RS14890 are separated by 165 nucleotides ABIE40_RS14890 and ABIE40_RS14895 are separated by 234 nucleotides
ABIE40_RS14885: ABIE40_RS14885 - alpha/beta hydrolase fold domain-containing protein, at 2,920,764 to 2,921,576
_RS14885
ABIE40_RS14890: ABIE40_RS14890 - LysR family transcriptional regulator, at 2,921,742 to 2,922,653
_RS14890
ABIE40_RS14895: ABIE40_RS14895 - SDR family oxidoreductase, at 2,922,888 to 2,923,790
_RS14895
Position (kb)
2921
2922
2923 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2920.788 kb on + strand at 2920.789 kb on - strand at 2920.789 kb on - strand at 2921.039 kb on - strand, within ABIE40_RS14885 at 2921.065 kb on + strand, within ABIE40_RS14885 at 2921.065 kb on + strand, within ABIE40_RS14885 at 2921.066 kb on - strand, within ABIE40_RS14885 at 2921.066 kb on - strand, within ABIE40_RS14885 at 2921.066 kb on - strand, within ABIE40_RS14885 at 2921.191 kb on + strand, within ABIE40_RS14885 at 2921.192 kb on - strand, within ABIE40_RS14885 at 2921.192 kb on - strand, within ABIE40_RS14885 at 2921.192 kb on - strand, within ABIE40_RS14885 at 2921.308 kb on + strand, within ABIE40_RS14885 at 2921.308 kb on + strand, within ABIE40_RS14885 at 2921.308 kb on + strand, within ABIE40_RS14885 at 2921.308 kb on - strand, within ABIE40_RS14885 at 2921.309 kb on - strand, within ABIE40_RS14885 at 2921.309 kb on - strand, within ABIE40_RS14885 at 2921.309 kb on - strand, within ABIE40_RS14885 at 2921.309 kb on - strand, within ABIE40_RS14885 at 2921.309 kb on - strand, within ABIE40_RS14885 at 2921.309 kb on - strand, within ABIE40_RS14885 at 2921.371 kb on + strand, within ABIE40_RS14885 at 2921.371 kb on + strand, within ABIE40_RS14885 at 2921.372 kb on - strand, within ABIE40_RS14885 at 2921.372 kb on - strand, within ABIE40_RS14885 at 2921.468 kb on - strand, within ABIE40_RS14885 at 2921.509 kb on + strand at 2921.585 kb on + strand at 2921.585 kb on + strand at 2921.585 kb on + strand at 2921.585 kb on + strand at 2921.585 kb on + strand at 2921.585 kb on + strand at 2921.585 kb on + strand at 2921.586 kb on - strand at 2921.586 kb on - strand at 2921.586 kb on - strand at 2921.586 kb on - strand at 2921.586 kb on - strand at 2921.586 kb on - strand at 2921.586 kb on - strand at 2921.586 kb on - strand at 2921.602 kb on + strand at 2921.603 kb on - strand at 2921.603 kb on - strand at 2921.647 kb on + strand at 2921.670 kb on - strand at 2921.710 kb on - strand at 2921.793 kb on - strand at 2921.798 kb on + strand at 2921.798 kb on + strand at 2921.799 kb on - strand at 2921.828 kb on + strand at 2921.829 kb on - strand at 2921.829 kb on - strand at 2921.829 kb on - strand at 2921.829 kb on - strand at 2921.895 kb on - strand, within ABIE40_RS14890 at 2921.895 kb on - strand, within ABIE40_RS14890 at 2921.934 kb on + strand, within ABIE40_RS14890 at 2921.934 kb on + strand, within ABIE40_RS14890 at 2921.934 kb on + strand, within ABIE40_RS14890 at 2921.935 kb on - strand, within ABIE40_RS14890 at 2921.935 kb on - strand, within ABIE40_RS14890 at 2921.938 kb on - strand, within ABIE40_RS14890 at 2922.022 kb on + strand, within ABIE40_RS14890 at 2922.022 kb on + strand, within ABIE40_RS14890 at 2922.023 kb on - strand, within ABIE40_RS14890 at 2922.023 kb on - strand, within ABIE40_RS14890 at 2922.084 kb on + strand, within ABIE40_RS14890 at 2922.084 kb on + strand, within ABIE40_RS14890 at 2922.252 kb on + strand, within ABIE40_RS14890 at 2922.252 kb on + strand, within ABIE40_RS14890 at 2922.252 kb on + strand, within ABIE40_RS14890 at 2922.253 kb on - strand, within ABIE40_RS14890 at 2922.306 kb on + strand, within ABIE40_RS14890 at 2922.306 kb on + strand, within ABIE40_RS14890 at 2922.306 kb on + strand, within ABIE40_RS14890 at 2922.307 kb on - strand, within ABIE40_RS14890 at 2922.307 kb on - strand, within ABIE40_RS14890 at 2922.319 kb on - strand, within ABIE40_RS14890 at 2922.396 kb on + strand, within ABIE40_RS14890 at 2922.396 kb on + strand, within ABIE40_RS14890 at 2922.396 kb on + strand, within ABIE40_RS14890 at 2922.396 kb on + strand, within ABIE40_RS14890 at 2922.396 kb on + strand, within ABIE40_RS14890 at 2922.396 kb on + strand, within ABIE40_RS14890 at 2922.397 kb on - strand, within ABIE40_RS14890 at 2922.397 kb on - strand, within ABIE40_RS14890 at 2922.397 kb on - strand, within ABIE40_RS14890 at 2922.397 kb on - strand, within ABIE40_RS14890 at 2922.397 kb on - strand, within ABIE40_RS14890 at 2922.398 kb on + strand, within ABIE40_RS14890 at 2922.398 kb on + strand, within ABIE40_RS14890 at 2922.398 kb on + strand, within ABIE40_RS14890 at 2922.398 kb on + strand, within ABIE40_RS14890 at 2922.398 kb on + strand, within ABIE40_RS14890 at 2922.398 kb on + strand, within ABIE40_RS14890 at 2922.399 kb on - strand, within ABIE40_RS14890 at 2922.399 kb on - strand, within ABIE40_RS14890 at 2922.399 kb on - strand, within ABIE40_RS14890 at 2922.399 kb on - strand, within ABIE40_RS14890 at 2922.399 kb on - strand, within ABIE40_RS14890 at 2922.399 kb on - strand, within ABIE40_RS14890 at 2922.399 kb on - strand, within ABIE40_RS14890 at 2922.552 kb on + strand, within ABIE40_RS14890 at 2922.552 kb on + strand, within ABIE40_RS14890 at 2922.553 kb on - strand, within ABIE40_RS14890 at 2922.553 kb on - strand, within ABIE40_RS14890 at 2922.553 kb on - strand, within ABIE40_RS14890 at 2922.553 kb on - strand, within ABIE40_RS14890 at 2922.553 kb on - strand, within ABIE40_RS14890 at 2922.716 kb on + strand at 2922.807 kb on - strand at 2922.807 kb on - strand at 2922.810 kb on + strand at 2922.810 kb on + strand at 2922.810 kb on + strand at 2922.810 kb on + strand at 2922.811 kb on - strand at 2922.811 kb on - strand at 2922.811 kb on - strand at 2922.811 kb on - strand at 2922.811 kb on - strand at 2922.811 kb on - strand at 2922.811 kb on - strand at 2922.811 kb on - strand at 2922.811 kb on - strand at 2922.812 kb on + strand at 2922.812 kb on + strand at 2922.812 kb on + strand at 2922.812 kb on + strand at 2922.813 kb on - strand at 2922.813 kb on - strand at 2922.852 kb on - strand at 2922.934 kb on + strand at 2922.934 kb on + strand at 2922.935 kb on - strand at 2923.128 kb on + strand, within ABIE40_RS14895 at 2923.138 kb on + strand, within ABIE40_RS14895 at 2923.138 kb on + strand, within ABIE40_RS14895 at 2923.138 kb on + strand, within ABIE40_RS14895 at 2923.139 kb on - strand, within ABIE40_RS14895 at 2923.153 kb on + strand, within ABIE40_RS14895 at 2923.154 kb on - strand, within ABIE40_RS14895 at 2923.276 kb on + strand, within ABIE40_RS14895 at 2923.277 kb on - strand, within ABIE40_RS14895 at 2923.318 kb on + strand, within ABIE40_RS14895 at 2923.318 kb on + strand, within ABIE40_RS14895 at 2923.318 kb on + strand, within ABIE40_RS14895 at 2923.318 kb on + strand, within ABIE40_RS14895 at 2923.318 kb on + strand, within ABIE40_RS14895 at 2923.319 kb on - strand, within ABIE40_RS14895 at 2923.319 kb on - strand, within ABIE40_RS14895 at 2923.465 kb on + strand, within ABIE40_RS14895 at 2923.465 kb on + strand, within ABIE40_RS14895 at 2923.466 kb on - strand, within ABIE40_RS14895 at 2923.466 kb on - strand, within ABIE40_RS14895 at 2923.466 kb on - strand, within ABIE40_RS14895 at 2923.466 kb on - strand, within ABIE40_RS14895 at 2923.466 kb on - strand, within ABIE40_RS14895 at 2923.630 kb on + strand, within ABIE40_RS14895
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 2,920,788 + -0.5 2,920,789 - +0.1 2,920,789 - +0.6 2,921,039 - ABIE40_RS14885 0.34 -1.2 2,921,065 + ABIE40_RS14885 0.37 -0.4 2,921,065 + ABIE40_RS14885 0.37 -0.4 2,921,066 - ABIE40_RS14885 0.37 -0.2 2,921,066 - ABIE40_RS14885 0.37 -0.4 2,921,066 - ABIE40_RS14885 0.37 -0.4 2,921,191 + ABIE40_RS14885 0.53 +1.0 2,921,192 - ABIE40_RS14885 0.53 -0.7 2,921,192 - ABIE40_RS14885 0.53 -0.6 2,921,192 - ABIE40_RS14885 0.53 +0.2 2,921,308 + ABIE40_RS14885 0.67 -0.1 2,921,308 + ABIE40_RS14885 0.67 +1.0 2,921,308 + ABIE40_RS14885 0.67 -1.4 2,921,308 - ABIE40_RS14885 0.67 +0.6 2,921,309 - ABIE40_RS14885 0.67 +0.9 2,921,309 - ABIE40_RS14885 0.67 +1.5 2,921,309 - ABIE40_RS14885 0.67 -0.0 2,921,309 - ABIE40_RS14885 0.67 +0.4 2,921,309 - ABIE40_RS14885 0.67 +0.5 2,921,309 - ABIE40_RS14885 0.67 +0.6 2,921,371 + ABIE40_RS14885 0.75 +0.4 2,921,371 + ABIE40_RS14885 0.75 +3.5 2,921,372 - ABIE40_RS14885 0.75 +0.5 2,921,372 - ABIE40_RS14885 0.75 +2.5 2,921,468 - ABIE40_RS14885 0.87 +0.4 2,921,509 + -2.2 2,921,585 + -1.6 2,921,585 + -0.9 2,921,585 + -0.5 2,921,585 + -2.1 2,921,585 + -0.6 2,921,585 + -0.4 2,921,585 + -1.0 2,921,586 - +0.5 2,921,586 - -0.5 2,921,586 - -0.2 2,921,586 - +0.3 2,921,586 - -0.0 2,921,586 - +0.3 2,921,586 - -0.4 2,921,586 - -0.8 2,921,602 + -1.5 2,921,603 - -0.9 2,921,603 - -0.4 2,921,647 + +0.5 2,921,670 - -1.6 2,921,710 - +0.6 2,921,793 - -0.1 2,921,798 + -0.5 2,921,798 + +0.5 2,921,799 - -1.4 2,921,828 + +0.1 2,921,829 - +1.0 2,921,829 - -2.3 2,921,829 - -0.9 2,921,829 - -1.7 2,921,895 - ABIE40_RS14890 0.17 +0.8 2,921,895 - ABIE40_RS14890 0.17 -0.8 2,921,934 + ABIE40_RS14890 0.21 -0.3 2,921,934 + ABIE40_RS14890 0.21 -0.0 2,921,934 + ABIE40_RS14890 0.21 -0.8 2,921,935 - ABIE40_RS14890 0.21 +0.7 2,921,935 - ABIE40_RS14890 0.21 -0.7 2,921,938 - ABIE40_RS14890 0.21 -0.6 2,922,022 + ABIE40_RS14890 0.31 -1.0 2,922,022 + ABIE40_RS14890 0.31 -0.3 2,922,023 - ABIE40_RS14890 0.31 -1.0 2,922,023 - ABIE40_RS14890 0.31 +1.0 2,922,084 + ABIE40_RS14890 0.38 -0.6 2,922,084 + ABIE40_RS14890 0.38 +1.0 2,922,252 + ABIE40_RS14890 0.56 -0.2 2,922,252 + ABIE40_RS14890 0.56 +1.5 2,922,252 + ABIE40_RS14890 0.56 -0.9 2,922,253 - ABIE40_RS14890 0.56 -0.7 2,922,306 + ABIE40_RS14890 0.62 -0.1 2,922,306 + ABIE40_RS14890 0.62 +0.1 2,922,306 + ABIE40_RS14890 0.62 -1.5 2,922,307 - ABIE40_RS14890 0.62 -1.0 2,922,307 - ABIE40_RS14890 0.62 -1.1 2,922,319 - ABIE40_RS14890 0.63 -1.3 2,922,396 + ABIE40_RS14890 0.72 +0.7 2,922,396 + ABIE40_RS14890 0.72 -0.2 2,922,396 + ABIE40_RS14890 0.72 +0.7 2,922,396 + ABIE40_RS14890 0.72 +0.8 2,922,396 + ABIE40_RS14890 0.72 +0.2 2,922,396 + ABIE40_RS14890 0.72 -3.0 2,922,397 - ABIE40_RS14890 0.72 -1.3 2,922,397 - ABIE40_RS14890 0.72 -0.6 2,922,397 - ABIE40_RS14890 0.72 -2.0 2,922,397 - ABIE40_RS14890 0.72 -2.1 2,922,397 - ABIE40_RS14890 0.72 -0.9 2,922,398 + ABIE40_RS14890 0.72 -0.5 2,922,398 + ABIE40_RS14890 0.72 -1.2 2,922,398 + ABIE40_RS14890 0.72 -1.0 2,922,398 + ABIE40_RS14890 0.72 +0.1 2,922,398 + ABIE40_RS14890 0.72 -0.0 2,922,398 + ABIE40_RS14890 0.72 -0.5 2,922,399 - ABIE40_RS14890 0.72 -1.5 2,922,399 - ABIE40_RS14890 0.72 -0.6 2,922,399 - ABIE40_RS14890 0.72 -0.9 2,922,399 - ABIE40_RS14890 0.72 -0.2 2,922,399 - ABIE40_RS14890 0.72 -0.1 2,922,399 - ABIE40_RS14890 0.72 -1.5 2,922,399 - ABIE40_RS14890 0.72 -1.2 2,922,552 + ABIE40_RS14890 0.89 -1.0 2,922,552 + ABIE40_RS14890 0.89 +0.0 2,922,553 - ABIE40_RS14890 0.89 -0.9 2,922,553 - ABIE40_RS14890 0.89 -0.4 2,922,553 - ABIE40_RS14890 0.89 -2.0 2,922,553 - ABIE40_RS14890 0.89 -0.9 2,922,553 - ABIE40_RS14890 0.89 -2.3 2,922,716 + +0.4 2,922,807 - +0.2 2,922,807 - -0.8 2,922,810 + -0.4 2,922,810 + -1.0 2,922,810 + -1.2 2,922,810 + +0.2 2,922,811 - +0.2 2,922,811 - -0.9 2,922,811 - -1.6 2,922,811 - +0.3 2,922,811 - +0.6 2,922,811 - -1.0 2,922,811 - +0.3 2,922,811 - +0.2 2,922,811 - +0.5 2,922,812 + +0.3 2,922,812 + -1.7 2,922,812 + -2.3 2,922,812 + -0.9 2,922,813 - +0.1 2,922,813 - +0.0 2,922,852 - +0.1 2,922,934 + +1.4 2,922,934 + +1.2 2,922,935 - -0.0 2,923,128 + ABIE40_RS14895 0.27 +0.4 2,923,138 + ABIE40_RS14895 0.28 -0.0 2,923,138 + ABIE40_RS14895 0.28 -1.8 2,923,138 + ABIE40_RS14895 0.28 +0.4 2,923,139 - ABIE40_RS14895 0.28 -1.4 2,923,153 + ABIE40_RS14895 0.29 +1.0 2,923,154 - ABIE40_RS14895 0.29 +0.4 2,923,276 + ABIE40_RS14895 0.43 +1.0 2,923,277 - ABIE40_RS14895 0.43 -0.1 2,923,318 + ABIE40_RS14895 0.48 +0.5 2,923,318 + ABIE40_RS14895 0.48 +0.1 2,923,318 + ABIE40_RS14895 0.48 +0.5 2,923,318 + ABIE40_RS14895 0.48 -0.1 2,923,318 + ABIE40_RS14895 0.48 +0.0 2,923,319 - ABIE40_RS14895 0.48 -1.1 2,923,319 - ABIE40_RS14895 0.48 -1.4 2,923,465 + ABIE40_RS14895 0.64 -0.2 2,923,465 + ABIE40_RS14895 0.64 -0.4 2,923,466 - ABIE40_RS14895 0.64 -1.0 2,923,466 - ABIE40_RS14895 0.64 +0.4 2,923,466 - ABIE40_RS14895 0.64 -0.1 2,923,466 - ABIE40_RS14895 0.64 -1.0 2,923,466 - ABIE40_RS14895 0.64 -0.4 2,923,630 + ABIE40_RS14895 0.82 +0.5
Or see this region's nucleotide sequence